• Title/Summary/Keyword: DOT-3

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Diagnostic value of a dot immunobinding assay for human pulmonary hydatidosis

  • OLUT Ali I.;ERGUVEN Sibel;EMRI Salih;OZUNLU Haluk;AKAY Hadi
    • Parasites, Hosts and Diseases
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    • v.43 no.1 s.133
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    • pp.15-18
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    • 2005
  • The diagnosis of human hydatidosis is primarily made using radiological and serological methods. Radiological methods are generally of low specificity and serological methods lack sensitivity, especially for pulmonary disease. In this study the capabilities of a new rapid test, the hydatid antigen dot immunobinding assay (HA-DIA), which was developed for the diagnosis of pulmonary hydatidosis, were studied and compared with another immunodiagnostic method, indirect hemagglutination (IHA). The study subjects included 18 patients, 9 women, 9 men; range 7 to 63 years; mean 30 years, with surgically proven pulmonary hydatidosis, a control group comprised of 14 patients; viral respiratory infections (1), cirrhosis (2), connective tissue disease (2), taeniasis (3), and 6 healthy donors. We found that the HA-DIA test had a sensitivity of $67\%$ and specificity of $100\%$, and that the IHA test had a sensitivity of $50\%$ and specificity of $100\%$. We conclude that HA-DIA is a simple, rapid, low cost assay that does not require instrumentation and has a higher sensitivity than IHA for the diagnosis of pulmonary hydatidosis.

Fabrication of micro injection mold with modified LIGA micro-lens pattern and its application to LCD-BLU

  • Kim, Jong-Sun;Ko, Young-Bae;Hwang, Chul-Jin;Kim, Jong-Deok;Yoon, Kyung-Hwan
    • Korea-Australia Rheology Journal
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    • v.19 no.3
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    • pp.165-169
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    • 2007
  • The light guide plate (LGP) of LCD-BLU (Liquid Crystal Display-Back Light Unit) is usually manufactured by forming numerous dots by etching process. However, the surface of those etched dots of LGP is very rough due to the characteristics of etching process, so that its light loss is relatively high due to the dispersion of light. Accordingly, there is a limit in raising the luminance of LCD-BLU. In order to overcome the limit of current etched-dot patterned LGP, micro-lens pattern was tested to investigate the possibility of replacing etched pattern in the present study. The micro-lens pattern fabricated by the modified LiGA with thermal reflow process was applied to the optical design of LGP. The attention was paid to the effects of different optical pattern type (i.e. etched dot, micro-lens). Finally, the micro-lens patterned LGP showed better optical qualities than the one made by the etched-dot patterned LGP in luminance.

Ferroelectric ultra high-density data storage based on scanning nonlinear dielectric microscopy

  • Cho, Ya-Suo;Odagawa, Nozomi;Tanaka, Kenkou;Hiranaga, Yoshiomi
    • Transactions of the Society of Information Storage Systems
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    • v.3 no.2
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    • pp.94-112
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    • 2007
  • Nano-sized inverted domain dots in ferroelectric materials have potential application in ultrahigh-density rewritable data storage systems. Herein, a data storage system is presented based on scanning non-linear dielectric microscopy and a thin film of ferroelectric single-crystal lithium tantalite. Through domain engineering, we succeeded to form an smallest artificial nano-domain single dot of 5.1 nm in diameter and artificial nano-domain dot-array with a memory density of 10.1 Tbit/$inch^2$ and a bit spacing of 8.0 nm, representing the highest memory density for rewritable data storage reported to date. Sub-nanosecond (500psec) domain switching speed also has been achieved. Next, long term retention characteristic of data with inverted domain dots is investigated by conducting heat treatment test. Obtained life time of inverted dot with the radius of 50nm was 16.9 years at $80^{\circ}C$. Finally, actual information storage with low bit error and high memory density was performed. A bit error ratio of less than $1\times10^{-4}$ was achieved at an areal density of 258 Gbit/inch2. Moreover, actual information storage is demonstrated at a density of 1 Tbit/$inch^2$.

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A highly sensitive molecular diagnosis method for detecting Toxoplasma gondii tachyzoite: a PCR/dot blot hybridization

  • Hong, Sun-Hwa;Lee, Yun-Seong;Kim, Young-Ho;Kim, Ok-Jin
    • Korean Journal of Veterinary Service
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    • v.37 no.1
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    • pp.29-33
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    • 2014
  • This study aimed at finding a fast, sensitive, and efficient protocol for molecular identification of intracellular protozoa Toxoplasma (T.) gondii. For molecular detection of T. gondii, we developed a polymerase chain reaction coupled with dot blot hybridization assay (PCR/DBH). For DBH analysis, the amplified DNA of T. gondii tachyzoite was labeled by incorporation of digoxigenin. The DBH assay alone was capable of detecting down to $1{\times}10^4$ pg of T. gondii genomic DNA. The PCR alone was capable of detecting down to $1{\times}10^3$ pg of T. gondii genomic DNA, whereas the PCR/DBH assay was capable of detecting down to $1{\times}10^2$ pg of T. gondii genomic DNA, indicating that sensitivity of the PCR/DBH method was approximately 10 to 100 times higher than PCR or DBH alone. Our PCR/DBH assay will be useful for confirming the presence of T. gondii on the samples and differentiating T. gondii infection from other intracellular protozoa infections.

CdSe Quantum Dots Sensitized TiO2 Electrodes for Photovoltaic Cells

  • Yum, Jun-Ho;Choi, Sang-Hyun;Kim, Seok-Soon;Kim, Dong-Yu;Sung, Yung-Eun
    • Journal of the Korean Electrochemical Society
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    • v.10 no.4
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    • pp.257-261
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    • 2007
  • The electronic properties of quantum dots can be tuned by changing the size of particles without any change in their chemical composition. CdSe quantum dots, the sizes of which were controlled by changing the concentrations of Cd and Se precursors, were adsorbed on $TiO_2$ photoelectrodes and used as sensitizers for photovoltaic cells. For applications of CdSe quantum dot as sensitizers, $CdSe/TiO_2$ films on conducting glass were employed in a sandwich-type cell that incorporated a platinum-coated conductive glass and an electrolyte consisting of an $I^-/I_3^-$ redox. The fill factor (FF) and efficiency for energy conversion ($\c{c}$) of the photovoltaic cell was 62 % and 0.32 %, respectively.

Selective Growth of Freestanding Carbon Nanotubes Using Plasma-Enhanced Chemical Vapor Deposition (플라즈마 기상 화학 증착법을 이용한 탄소나노튜브의 선택적 수직성장 기술)

  • Bang, Yun-Young;Chang, Won-Seok
    • Journal of the Korean Society for Precision Engineering
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    • v.24 no.6
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    • pp.113-120
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    • 2007
  • Chemical vapor deposition (CVD) is one of the various synthesis methods that have been employed for carbon nanotube (CNT) growth. In particular, Ren et al reported that large areas of vertically aligned multi-wall carbon nanotubes could be grown using a direct current (dc) PECVD system. The synthesis of CNT requires a metal catalyst layer, etchant gas, and a carbon source. In this work, the substrates consists of Si wafers with Ni-deposited film. Ammonia $NH_3$) and acetylene ($C_2H_2$) were used as the etchant gases and carbon source, respectively. Pretreated conditions had an influence on vertical growth and density of CNTs. And patterned growth of CNTs could be achieved by lithographical defining the Ni catalyst prior to growth. The length of single CNT was increased as niclel dot size increased, but the growth rate was reduced when nickel dot size was more than 200 nm due to the synthesis of several CNTs on single Ni dot. The morphology of the carbon nanotubes by TEM showed that vertical CNTs were multi-wall and tip-type growth mode structure in which a Ni cap was at the end of the CNT.

Design and Implementation of Smart Pen based User Interface System for U-learning (U-Learning 을 위한 스마트펜 인터페이스 시스템 디자인 및 개발)

  • Shim, Jae-Youen;Kim, Seong-Whan
    • Proceedings of the Korea Information Processing Society Conference
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    • 2010.11a
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    • pp.1388-1391
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    • 2010
  • In this paper, we present a design and implementation of U-learning system using pen based augmented reality approach. Student has been given a smart pen and a smart study book, which is similar to the printed material already serviced. However, we print the study book using CMY inks, and embed perceptually invisible dot patterns using K ink. Smart pen includes (1) IR LED for illumination, IR pass filter for extracting the dot patterns, and (3) camera for image captures. From the image sequences, we perform topology analysis which determines the topological distance between dot pixels, and perform error correction decoding using four position symbols and five CRC symbols. When a student touches a smart study books with our smart pen, we show him/her multimedia (visual/audio) information which is exactly related with the selected region. Our scheme can embed 16 bit information, which is more than 200% larger than previous scheme, which supports 7 bits or 8 bits information.

Detection of antibodies and antigens of Aujeszky's disease virus using dot-immunoassay (Dot-immunoassay를 이용한 Aujeszky's disease virus항원 및 항체 검출)

  • Jun, Moo-hyung;Cho, Young-sung;Chang, Kyung-soo
    • Korean Journal of Veterinary Research
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    • v.34 no.3
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    • pp.529-536
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    • 1994
  • To establish more specific and simple diagnostic methods for detection of the antibodies and antigens of Aujeszky's disease virus(ADV), we designed indirect dot-immunoassay(IDI) and double sandwich dotimmunoassay(DSDI) using the solid phases of nitrocellolose paper and polystyrene plate. The diagnostic efficacy of these methods was investigated. As the sensitivity of IDI was tested by various virus concentration, the specimens with the virus titer above $10^{4.0}TCID_{50}/0.2ml$ showed positive reaction, but that below $10^{1.0}TCID_{50}/ml$ revealed negative. Tonsil emulsion at the virus titer of $10^{4.5}TCID_{50}/0.2ml$ showed the highest sensitivity as diluted by 1/100. In detection of ADV antigens from the various tissues of the rats and pigs infected with ADV, IDI using monoclonal antibody showed the higher specificity as compared with IDI using polyclonal antibody and virus isolation method. The efficacy of the DSDI for detection of ADV antibody was compared with other tests. The sensitivity of DSDI was higher than virus neutralization(VN) and agar gel immunodiffusion test(AGID). Meanwhile, specificity of DSDI was lower than AGID, but similar to IDEA. In comparison with VN test, DSDI showed 96.9% agreement to VN test that is the highest of three tests. In general, application of polyclonal antibody in both tests caused the higher sensitivity but the lower specificty.

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Effects of Physical Training on Defence Mechanism of Aging and Memory Impairment of Senescence-accelerated SAMP8 (운동이 SAMP8 마우스의 노화와 기억장애에 미치는 영향)

  • Ku, Woo-Young;Lee, Jong-Soo;Kwak, Yi-Sub
    • IMMUNE NETWORK
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    • v.5 no.4
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    • pp.252-257
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    • 2005
  • Background: This study was designed to investigate the effect of exercise training on defense mechanism of chronic degenerative disease, aging, and memory impairments of senescence-accelerated mouse (SAM)P8 under the hypothesis that "Senile dementia may be prevented by regular exercises". Methods: To evaluate the effects of exercise training on the defense mechanism of aging and memory impairment, SAMP8 were divided into two groups, the control group and exercise training groups. the exercise training group were performed with low $(\dot{V}O_2max\;25{\sim}33%)$, middle ($\dot{V}O_2max$ 50%) and high $(\dot{V}O_2max\;66{\sim}75%)$ intensity exercise. All SAMP8 mice were fed experimental diet ad libitum until 4, 8 months, and dead period. Results: Median lifespan in middle exercise group resulted in a significantly increased (23.5% and 18.7%, respectively), whereas these lifespan in high exercise group resulted in an unexpectedly decreased (13.5% and 12.1%, respectively) compared with control group. Body fat levels in 4 and 8 months of age were significantly decreased 43% to 51% in middle exercise group, whereas were remarkably deceased to 57% in high exercise group compared with control group. It is believed that extended median and maximum lifespan may be effected by calory restriction through the exercise training. Acetylcholine (ACh) levels were significantly increased 6.7% and 8.5% in middle and high exercise groups, and also choline acetyltransfease (ChAT) activities were significantly increased 10.3% and 11.9% in middle and high exercise groups. Conclusion: These results suggest that proper and regular exercises such as middle group ($\dot{V}O_2max$ 50%) may play an effective role in attenuating an oxygen radicals and may play an important role in improving a learning and memory impairments of senile dementia.

Error-Diffusion Technique using Variable Principle Distances for LCD Monitor (액정디스플레이를 위한 가변 주거리 기반의 오차 확산 기법)

  • Yoon, Jo-Seph;Park, Gyeong-Mi;Kim, Young-Bong
    • Journal of Korea Multimedia Society
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    • v.12 no.3
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    • pp.362-371
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    • 2009
  • The key technology for developing high quality LCDs is about manufacturing BLUs with homogeneous dot distributions. Commonly, homogeneous dot distributions are obtained by the halftoning methods which convert a gray-scale image to a binary image. Among many halftoning algorithms, the error-diffusion technique based on the principle distance is known to show homogeneous dot distributions. However, this technique has a drawback; the extent of the principle distance at each pixel with respect to those of the neighboring pixels can be too small or big creating a gap or overlap. In this paper, we propose a new error diffusion algorithm based on the variable principle distance which improves the existing error diffusion technique based on the principle distance. The variable principle distance at a given pixel is calculated with gray-scale values of the pixel and its neighbors and thus the principle distance value is variable depending on the direction from that pixel. This variable principle distance technique helps BLUs obtain homogeneous dot distributions.

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