• Microbiology and Biotechnology Letters
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• v.25 no.5
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• pp.483-489
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• 1997

Sulforhodamine B (SRB) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, RNA dot blot and Northern hybridization analysis were performed to screen microorganisms for the production of anticancer agent. Among microorganisms tested, strain YP-1 was selected for its cytotoxicity and ability to reduce the level of c-myc RNA. Strain YP-1 was identified as Streptomyces endus. The anticancer material produced by Streptomyces endus YP-1 was sequentially purified by solvent extraction, silica gel column chromatograpby, preparative TLC and preparative HPLC. The cancer material identified as azalomycin B by the instrumental analyses such as $^{1}$H-NMR, $^{13}$C-NMR, Mass, IR and UV absorption. It was colorless amorphous powder and its molecular weight was 1025.278. Azalomycin B, produced by Streptomyces endus YP-1, showed anticancer activity against several human cancer cell lines and reduction of c-Myc protein level in Colo320 DM cells which was determined by Western blot analysis.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2006.05a
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• pp.95-98
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• 2006

LCD-BLU (Back Light Unit) is one of kernel parts of LCD unit. The fabrication method of a 3-D micro mold patterned with micro-lenses for the LGP (Light Guiding Plate), one of the most important parts of LCD-BLU, was presented. Instead of dot pattern made by etching, 3-D optical pattern design with $50{\mu}m$ micro-lens was applied in the present study. The micro-lens pattern fabricated by modified LiGA with thermal reflow process was applied to the optical design of LGP. The positive micro-lens patterned injection mold with different aspect ratios (i.e. 0.3 and 0.4) was fabricated with modified LiGA with thermal reflow process. The brightness of LCD-BLU increased as aspect ratio of micro-lens increased.

• Journal of The Korean Astronomical Society
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• v.46 no.6
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• pp.235-242
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• 2013

We compare mass-loss rates of OH/IR stars obtained from radio observations with those derived from the dust radiative transfer models and IR observations. We collect radio observational data of OH maser and CO line emission sources for a sample of 1533 OH/IR stars listed in Suh & Kwon (2011). For 1259 OH maser, 76 CO(J=1-0), and 55 CO(J=2-1) emission sources, we compile data of the expansion velocity and mass-loss rate. We use a dust radiative transfer model for the dust shell to calculate the mass-loss rate as well as the IR color indices. The observed mass-loss rates are in the range predicted by the theoretical dust shell models corresponding to $\dot{M}=10^{-8}M_{\odot}/yr-10^{-4}M_{\odot}/yr$. We find that the dust model using a simple mixture of amorphous silicate and amorphous $Al_2O_3$ (20% by mass) grains can explain the observations fairly well. The results indicate that the dust radiative transfer models for IR observations generally agree with the radio observations. For high mass-loss rate OH/IR stars, the mass-loss rates obtained from radio observations are underestimated compared to the mass-loss rates derived from the dust shell models. This could be because photon momentum transfer to the gas shell is not possible for the physical condition of high mass-loss rates. Alternative explanations could be the effects of different dust-to-gas ratios and/or a superwind.

• Fashion & Textile Research Journal
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• v.8 no.5
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• pp.585-590
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• 2006

In the variation of physical properties of adhesive fabrics, the objective of this study is to analyze fitness of fusible interlining to four sorts of fabrics for men's suit with various structural parameters. Four fusible interlinings are used for adhering to fabrics for men's suit. Mechanical properties of these 16 adhesive fabrics fused with these interlinings are measured by KES-FB system for analysing appearance and wearing properties of garments and for examining the fitness of fusible interlining to the fabrics for men's suit. The variation of bending property for adhesive fabrics are found to be higher in the order of interlining 1(Cotton30/Rayon70, plain), interlining 2(Polyester30/Rayon70, warp knit), interlining 4(Polyester, shingosen fabrics with drawing textured yarn) and interlining 3(Polyester, double dot coating interlining). The variation of adhesive effect shows the same results with bending property and shows negative relation with extensibility in weft direction per extensibility in warp direction(EM2/EM1). The variation of peel strength of adhesive fabrics, woolen fabrics in the case of face fabrics and interlining 3 in the case of interlining shows the largest value respectively.

• Journal of the Korean Magnetics Society
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• v.6 no.4
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• pp.199-203
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• 1996

To study the magnetization reversal behavior of Co/Pd multilayers, we first demagnetized the samples by the field-demagnetized method and then measured initial curves and minor loops. The coercivity and the perpendicular magnetic anisotropy were obtained from the perpendicular and parallel magnetization curves measured at different temperatures. We interpret our experimental results by applying several qualitative and semiquantative approaches. From these study, we found that the magnetization reversal behavior is dominated by the domain wall pinning for all samples and the coercivity incremental tendency can be explained by Kronmuller's formula $H_c(T)\;{\propto}\;r_0.K_u$.

• Structural Engineering and Mechanics
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• v.66 no.2
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• pp.185-193
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• 2018

In this study, low- and high-frequency structure behaviors were identified and a systematic analysis procedure was proposed using noisy GPS data from a 165-m-high tower in ${\dot{I}}stanbul$, Turkey. The raw GPS data contained long- and short-periodic position changes and noisy signals at different frequencies. To extract the significant results from this complex dataset, the general structure and components of the GPS signal were modeled and analyzed in the time and frequency domains. Uncontrolled jumps and deviations involving the signal in the time domain were pre-filtered. Then, the signal was converted to the frequency domain after applying low- and high-pass filters, and the frequency and periodic component values were calculated. The spectrum of the tower motion obtained from the filtered GPS data had dominant peaks at a low frequency of $1.15572{\times}10-4Hz$ and a high frequency of 0.16624 Hz, consistent with two equivalent GPS datasets. Then, the signal was reconstructed using inverse Fourier transform with the dominant low frequency values to obtain filtered and interpretable clean signals. With the proposed sequence, processing of noisy data collected from the GPS receivers mounted very close to the structure is effective in revealing the basic behaviors and features of buildings.

• Journal of Korea Game Society
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• v.6 no.4
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• pp.79-87
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• 2006

This paper proposes the design and implementation of engine to control characters by using machine teaming techniques. Because the proposed engine uses the context data in the rum time as the knowledge data, there is a merit which the player can not easily recognize the behavior pattern of the intelligent character. To do this, the paper proposes to develop the module which gathers and trains the context data and the module which tests to decide the optimal context control for the given context data. The developed engine is ported to FEAR and run with Quake2 and experimented far the correctness of the development and its efficiency. The experiments show that the developed engine is operated well and efficiently within the limited time.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.21 no.1
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• pp.1-6
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• 2012

This paper presents a stress safety of a composite pressure cylinder for a hydrogen gas vehicle. The composite pressure cylinder in which is composed of an aluminum liner and carbon fiber wound layers contains 104 liter hydrogen gas, and is compressed by a filling pressure of 70 MPa. The FEM computed results are analyzed based on the US DOT-CFFC basic requirement for a hydrogen gas cylinder and KS B ISO specification. The FEM results indicate that the stress, 255.2 MPa of an aluminum liner is sufficiently low compared with that of 272 MPa, which is 95% level of a yield stress for aluminum. Also, the composite layers in which are wound on the surface of an aluminum cylinder are safe because the stress ratios from 3.46 to 3.57 in hoop and helical directions are above 2.4 for a minimum safety level. The proposed composite pressure cylinder wound by carbon fibers is useful for 70 MPa hydrogen gas vehicles.

• Journal of Sensor Science and Technology
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• v.31 no.2
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• pp.125-130
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• 2022

We describe the importance of zinc oxide nanoparticle (ZnO NP) concentration in the enhancement of electrical properties in a lead sulfide quantum dot (PbS QD)-based shortwave infrared (SWIR) photodetector. ZnO NPs were synthesized using the sol-gel method. The concentration of the ZnO NPs was controlled as 20, 30 and 40 mg/mL in this study. Note that the ZnO NPs layer is commonly used as an electron transport layer in PbS QDs SWIR photodetectors. The photo-to-dark ratio, which is an important parameter of a photodetector, was intensively examined to evaluate the electrical performance. The 20 mg/mL condition of ZnO NPs exhibited the highest photo-to-dark ratio value of 5 at -1 V, compared with 1.8 and 0.4 for 30 mg/mL and 40 mg/mL, respectively. This resulted because the electron mobility decreased when the concentration of ZnO NPs was higher than the optimized value. Based on our results, the concentration of ZnO NPs was observed to play an important role in the electrical performance of the PbS QDs SWIR photodetector.

• Journal of Plant Biotechnology
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• v.35 no.1
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• pp.87-94
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• 2008

Porcine epidemic diarrhea virus (PEDV) is an infectious and highly contagious virus of swine. In order to develop the transgenic tobacco culture cells producing PEDV antigen protein, four vectors expressing PEDV spike protein (SP) gene under the control of a CaMV 35S promoter were constructed. Four fragments of the SP region of PEDV, SP1 (444 bp, 1487-1930 bp), SP2 (1.7 kb, 2300-3987 bp), SP3 (1.4 kb, 1559-2950 bp), and SP4 (2.6 kb, 9-2643 bp) were amplified by PCR and then C-MYC tag was fused to the end of each SP gene, respectively. These cassettes are inserted into the pCAMBIA2300 (named as 35S::SP1-M, 35S::SP2-M 35S::SP3-M, and 35S::SP4-M, respectively). Tobacco (cv. BY-2) cultured cells were transformed by co-cultivation with Agrobacterium tumefaciens harboring expression vector. We selected kanamycin-resistant calli and checked for the presence of the introduced SP gene using PCR, resulting 70% of them showed the foreign gene. We selected the lines with high-level expression of PEDV antigen protein based on dot blot analysis. Southern blot analysis confirmed that the PEDV SP gene was integrated into the genome of the tobacco cultured cells. Northern blot analysis showed that the introduced gene was highly expressed in transgenic cultured cells. Transgenic tobacco cultured cells-derived antigen induced immunogenicity in mice as determined by a plaque reduction neutralization assay. These results suggest that the vectors expressing PEDV spike protein gene in this study will be useful for the development of transgenic plants and cultured cells producing PEDV antigene protein.