• Biomolecules & Therapeutics
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• v.7 no.2
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• pp.185-190
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• 1999

The current study was performed to determine the acute oral toxicity of P55A, a crude extract of 1 : 1 mixture of Phellodendron amurense cortex and Aralia elata cortex, in SD rats and beagle dogs. 5 rats of each sex were treated with a single dose of P55A orally at doses of 0 and 5,000 mg/kg respectively. Also 2 dogs of each sex were treated with a single dose of P55A orally at doses of 0 and 2,000 mgAg, respectively. After the treatment, clinical signs, and body weight change were observed for 14 days. All rats survived during the study and did not show any clinical sign. Body weight gain showed no significant difference between the control and treated rats. Grossly, no lesion was observed in the rats. All dogs survived during the study. In clinical signs, dark stool was observed in the 2,000 mg/kg treated dogs at day 1 after administration. The animals recovered from general signs at day 2 after administration. Body weight gain showed no significant difference between the control and treated dogs. Grossly, no lesion was observed in the dogs. It is suggested that the LD$_{50}$ of P55A by oral administration was estimated to be over 5,000 mg/kg in both sexes of rats and 2,000 mg/kg in both sexes of beagle dogs.s.

• Journal of Veterinary Clinics
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• v.15 no.2
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• pp.331-338
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• 1998

This study evaluated the effect of acupuncture in dogs with experimentally induced spleens pancreas and urinary bladder disease. For the spleen studys sixteen dogs were divided into four groups(each of 4 in dogs): laserfonctnreg electroacupunctnre, acupuncture and control group. Spleen disease was brought by experimentally induced spleen traumata and then treatment was done once daisy for 4 days. The acupoints used were Pi-shu and Ge-shu. The effect of acupuncture was evaluated by clinical grmptomi hematological findings and blood chemical values. Acupuncture and Iaserpunctore group revealed rotatively fast recovery compared with other sloops. For the pancreas study, sixteen dogs were divided into four groups(each of 4 in dogs): laserpuncture, electroacupuncture, acupuncture and control group. Pancreas disease was brought by experimentally induced pancreas trauma, and then treatment was done once daily for 4 days The acupoints used were Wei-shu and Pi-shu. The effect of acupuncture was evaluate6 by clinical symptom, hematological findings and blood chemical values. Laserpuncture revealed relatively frost recovery and acupuncture group revealed secondly fast recovery after treatment. For the urinary bladder study, sixteen dogs were divided into four groups(each of 4 in dogs): laserpuncture, electroacupuncture,acupuncture and control group. Spleen disease was brought by experimentally induced urinary stoner and then treatment was done once daily for 4 days. The acupuncture used were Pang Guang-shu and Shen-shu. The effect of acupuncture was evaluated by clinical symptom, hematological findings, and blood chemical values. Laserpuncture and acupuncoure group revealed relatively fast recovery compared with other groups.

• Korean Journal of Veterinary Service
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• v.39 no.2
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• pp.69-74
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• 2016

Jeju Dogs have high academic value due to their unique characteristics and conditions, but they have received little attention in the research field. This paper attempts to secure fundamental data to clarify the general characteristics of Jeju Dogs. For this purpose, an inspection of the basic body shape was performed to establish the shape and breed standards. Jeju Dogs were also exposed to 12 behavior tests to check their distinct character categories and an objective ethogram was built on each of the character categories to conduct a behavior analysis. The body shape of 34 clinically healthy Jeju Dogs aged 18 months or older was inspected using ten divided parts. For the behavior analysis, 10 Jeju Dogs were examined. A variety of behavioral variables were recorded based on 12 behavior tests that were categorized into four characters: sociality, aggressiveness, anxiety and submissiveness. The results of the body shape inspection indicated that most of the male dogs' parts had larger measured values than the female dogs' parts. The behavior analysis results revealed that the Jeju Dog displays excellent qualities such as submissiveness, reliability and fidelity and, hence, it is believed that this breed of dog is very appropriate for most duties and has the potential to be a household dog or working dog.

• Journal of Veterinary Clinics
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• v.26 no.6
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• pp.632-636
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• 2009

Three dogs presented to the Veterinary Medical Teaching Hospital of the University of Konkuk and Hangang Animal Hospital with a history of perineal swelling. No tenesmus, stranguria, or any clinical signs other than the swelling was observed by the owners in three dogs. On physical examination, the swelling was observed unilaterally in two dogs and bilaterally in a dog. Digital palpation to the swelling confirmed reducible perineal herniation in two dogs and irreducible perineal herniation in a dog. Plain radiographs revealed that no pelvic or abdominal contents other than the fat were displaced into subcutaneous perineal region in three dogs. Internal obturator transposition herniorrhaphy was performed for correction of perineal herniation in three dogs. Contralateral herniation involving fat was noted after surgery in a dog. The follow-up information was based on physical examination by veterinarians or telephone interview with owners. The owners reported that there was no evidence of complications related to surgery such as sciatic nerve injury, rectal prolapse, wound dehiscence, or perineal hernia recurrence in all dogs.

• Journal of Veterinary Clinics
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• v.18 no.3
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• pp.243-248
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• 2001

Radiographic findings of degenerative joint disease (DJD) in the coxofemoral joints were studied in 108 dogs referred to Veterinary Medical Teaching Hospital, College of Veterinary Medicine, Seoul National University. Radiographic findings were identified with a extended ventrodorsal projection. Clinical signs such as pain in the affected joint, exercise intolerance, lameness, crepitation on flexion and extension of the joint and decreased range of motion, were shown in 40% and radiographic changes of bilateral coxofemoral joints were identified in 69% of a group of dogs. There are no sex predilections. Thirty-seven dogs were diagnosed as canine hip dysplasia (CHD; 10 cases), avascular necrosis of the femoral head (Legg-Calves-Perthes disease [LCPD]; 12 cases), fracture of the acetabulum (1 case), luxation(10 cases) and subluxation (15 cases), however, luxation and subluxation were identified in 11 dogs affected with CHD and LCPD. Causes of DJD in others (71case) were not identified. Radio-graphic findings identified included osteophyte formation at the attachment of the joint capsule (70%), sclerosis of the subchondral bone of the cranial acetabular edge (47%), remodeling of the femoral head (34%), thickening of the femoral neck(31%), perichondral osteophyte formation (31%), joint laxity (30%) and so on. All of the dogs diagnosed as CHD were large breed such as Retriever, Germain Shepherd, and Weimaraner and their age was under 1 year. This condition occurred bilaterally in 70% of affected animals. Dogs diagnosed as LCPD were young (under 1 year), small breed dogs. The incidence of LCPD is greater in Yorkshire Terrier, Miniature Pinscher and Poodle. This condition occurred unilaterally in 67% of affected animals. Clinical signs of dogs with unknown cause were shown in 15% and this condition occurred bilaterally in 75% of affected animals. Radiographic findings varied with patient's age and breed, however, the most common finding was osteophyte formation at the attachment of the joint capsule.

• Journal of Veterinary Clinics
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• v.24 no.2
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• pp.160-163
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• 2007

Patients often present with spontaneous bleeding, or a bleeding disorder may be discovered when an otherwise healthy dog develops marked bleeding during or after surgery. In this study, we were aimed to elucidate whether the cesarean section in dogs has influence on the coagulation profiles. And we gained the normal data on a panel of screening laboratory tests which allow accurate characterization of a hemostatic defects in dogs. Of the 20 healthy adult dogs, buccal mucosa bleeding time (BMBT) was $83.0{\pm}10.5$ seconds, platelet count was $24.0{\pm}3.5{\times}10^4/{\mu}l$, activated partial thromboplastin time (APTT) was $8.8{\pm}2.0$ seconds, the concentration of fibrinogen was $288.5{\pm}77.9mg/dl$, and the concentration of fibrin degradation products (D-dimer) was <250.0 ng/ml. Coagulation profiles before and after cesarean section of 13 cesarean sectioned dogs were in the normal range and there were no statistical differences in coagulation profiles between normal dogs and cesarean sectioned dogs (p>0.05). The results suggested that labor and cesarean section in healthy dogs did not alter coagulation profiles.

• Journal of Veterinary Clinics
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• v.34 no.1
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• pp.27-33
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• 2017

Although HDACIs affect ubiquitously expressed histone deacetylase and increase cellular senescence, there has been little study on the effect of age on treatment with HDACIs. Accordingly, the purpose of this study was to compare cellular senescence status and vorinostat-induced senescence in fibroblasts derived from aged dogs compared to young dogs. Skin tissues were taken from young (1-year-old) and aged (7-year-old) male dogs, and fibroblasts were cultured without (control) or with 10 uM of vorinostat for 24 hr. Beta-galactosidase activity was assessed, and real-time polymerase chain reaction and western blotting were performed to analyze the expression levels of transcripts and proteins related to cellular senescence. Beta-galactosidase activity was higher in aged dogs compared to young dogs in the control group, and was increased by vorinostat treatment. Expression of p21, p53 and p16 transcripts was higher in the aged than in the young group, and all transcripts were affected by vorinostat in both young and aged groups. Western blot results showed lower H3K9 acetylation in the aged dogs compared to the young dogs, and the acetylation was increased by vorinostat treatment in both groups. However, there was no significant difference between the transcript or protein alterations induced by vorinostat.

• Korean Journal of Veterinary Research
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• v.60 no.1
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• pp.9-14
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• 2020

This study describes pathological, immunohistochemical, and bacteriological findings in adult dogs and fetuses naturally infected with Brucella (B.) canis. A total of 42 dogs including 40 dogs and 2 aborted fetuses were examined. The most common gross lesions in infected dogs were swelling of lymph nodes and spleen. The testes showed marked swelling with multifocal to diffuse reddish discoloration. The most significant histopathological lesions were observed in the placenta. Placental trophoblasts were markedly hypertrophied due to the accumulation of intra-cellular gram-negative bacteria. Lymphocytic inflammation of varying severity was observed in the reproductive organs such as male testis, epididymis, and prostate gland and female uterus. Strong immunolabelling was observed in the cytoplasm of most trophoblasts in the placental tissues using immunohistochemistry. However, immunohistochemical staining did not demonstrate any organisms in other organs of dogs and fetuses. B. canis isolates were most frequently obtained from the whole blood (82.5%) and superficial inguinal lymph node (77.5%) in both sexes. In addition, the isolation rate was higher in male genital organs than in those of females. Hence, management of male dogs is most important because infected dogs can play a role as carriers.

• Journal of Veterinary Science
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• v.22 no.1
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• pp.2.1-2.10
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• 2021

Background: Diseases related to cerebrospinal fluid flow, such as hydrocephalus, syringomyelia, and Chiari malformation, are often found in small dogs. Although studies in human medicine have revealed a correlation with cerebrospinal fluid flow in these diseases by magnetic resonance imaging, there is little information and no standard data for normal dogs. Objectives: The purpose of this study was to obtain cerebrospinal fluid flow velocity data from the cerebral aqueduct and subarachnoid space at the foramen magnum in healthy beagle dogs. Methods: Six healthy beagle dogs were used in this experimental study. The dogs underwent phase-contrast and time-spatial labeling inversion pulse magnetic resonance imaging. Flow rate variations in the cerebrospinal fluid were observed using sagittal time-spatial labeling inversion pulse images. The pattern and velocity of cerebrospinal fluid flow were assessed using phase-contrast magnetic resonance imaging within the subarachnoid space at the foramen magnum level and the cerebral aqueduct. Results: In the ventral aspect of the subarachnoid space and cerebral aqueduct, the cerebrospinal fluid was characterized by a bidirectional flow throughout the cardiac cycle. The mean ± SD peak velocities through the ventral and dorsal aspects of the subarachnoid space and the cerebral aqueduct were 1.39 ± 0.13, 0.32 ± 0.12, and 0.76 ± 0.43 cm/s, respectively. Conclusions: Noninvasive visualization of cerebrospinal fluid flow movement with magnetic resonance imaging was feasible, and a reference dataset of cerebrospinal fluid flow peak velocities was obtained through the cervical subarachnoid space and cerebral aqueduct in healthy dogs.

• Parasites, Hosts and Diseases
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• v.57 no.2
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• pp.197-200
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• 2019

Cryptosporidium is a common intestinal protozoan that can lead to diarrhea in humans and dogs. The predominant species of infection are C. hominis and C. parvum in humans, and C. canis in dogs. However, C. canis can infect immunocompromised humans. Considering the close contact with humans, dogs have the potential to be reservoirs for human cryptosporidiosis. Breeding kennels are the major supply source of puppies for pet shops. The present study is to determine the molecular prevalence and characteristics of Cryptosporidium spp. found in breeding kennel dogs. A total of 314 fecal samples were collected from young and adult dogs kept in 5 breeding kennels. A polymerase chain reaction targeting the small subunit rRNA gene was employed for the detection of Cryptosporidium spp. To determine the species, the DNA sequences were compared to GenBank data. Overall, 21.0% of the fecal samples were positive for Cryptosporidium spp. infection. Cryptosporidium spp. was detected in all 5 facilities. A sequencing analysis demonstrated that all isolates shared 99-100% similarity with C. canis. The results suggest that Cryptosporidium spp. infection is present at a high-level in breeding kennel dogs. However, because dominant species in this survey was C. canis, the importance of breeding kennel dogs as reservoirs for Cryptosporidium spp. transmission to humans is likely to be low in Japan.