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The Growth and Physiological Responses of Cacalia firma Seedlings by Shading Conditions in Forest Farming (임간재배 시 병풍쌈 유묘의 차광처리별 생장 및 생리 반응)

  • Yoon, Jun Hyuck;Jeon, Kwon Seok;Song, Ki Seon;Park, Yong Bae;Moon, Yong Sun;Lee, Do Hyung
    • Journal of Korean Society of Forest Science
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    • v.103 no.1
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    • pp.65-71
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    • 2014
  • Cacalia firma is a perennial plant in Asteraceae, Parasenecio that distributed in Korea, China, and Japan. As dietary style changes for well-being life, consumer's demand of functional food and organic vegetables is getting increased. This study was conducted to investigate the optimum light conditions of P. firmus in forest farming. One year old seedlings were grown under four different light conditions 10%, 20%, 30%, and 50% of sunlight by shading (equals 50%, 30%, 20%, and 10% relative brightness respectively) and non-treated control under full sunlight. They were analyzed for early growth and physiological response. Seedlings grown under 75% shading showed similar height, root growth, and leaf water content to control. However, their leaf length, width, and total leaf area were increased, which caused increased leaf dry weight and total dry weight. Especially, seedlings under 95% shading showed 40% increase in height and more leaf growth and leaf water content, although they had shorter main root length and root collar diameter than control. In addition specific leaf area (SLA) and leaf area ratio (LAR) were higher than control and indicated that they were statistically significant difference from control. Higher SLA refers thinner leaf thickness, higher LAR means larger leaf area. The results indicate seedlings under 95% shading have higher water content, thinner leaf, and wider lightinterception areas. It is plausible that P. firmus is active in chlorophyll activities and carbon dioxide assimilation at even lower light conditions. These results suggest that the optimum light level of P. firmus for artificial cultivation in forest farming ranges from 75~95% shading (20%-10% of relative brightness). When salability as 'sanchae' (wild edible greens) is considered, P. firmus could be cultivated under 75% shading in forest farming and expected to have better taste and higher yield. We suggest these results as basic data of P. firmus for possible forest farming.

Application of Reused Powdered Waste Containing Aluminum Oxide on the Treatment of Cr(VI) (6가 크롬 처리를 위한 알루미늄 산화물을 함유한 재생 분말 폐기물의 적용)

  • Lim, Jae-Woo;Kim, Tae-Hwan;Kang, Hyung-Sik;Kim, Do-Son;Kim, Han-Seon;Cho, Seok-Hee;Yang, Jae-Kyu;Chang, Yoon-Young
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.3
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    • pp.179-185
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    • 2009
  • In this research, the removal capacity of Cr(VI) by the reused powdered wastes (RPW) containing aluminium oxides was studied. As a pre-treatment process for the preparation of calcined wastes, calcination was conducted at $550^{\circ}C$ to remove organic fraction in the raw wastes. In order to study the adsorption trend of Cr(VI) ions from aqueous solutions, the pH-edge adsorption, adsorption kinetic and adsorption isotherm were investigated using a batch reactor in the presence of four different background electrolytes($NO_3\;^-,\;CO_3\;^{2-},\;SO_4\;^{2-},\;PO_4\;^{3-}$). Cr(VI) adsorption was greatly reduced in the presence of $SO_4\;^{2-}$ and $PO_4\;^{3-}$ over the entire pH range. Meanwhile the inhibition effect by $NO_3\;^-$ and $CO_3\;^{2-}$ was relatively lower than that by $SO_4\;^{2-}$ and $PO_4\;^{3-}$. Cr(VI) adsorption was maximum around pH 4.5 in the presence of $NO_3\;^-$ and $CO_3\;^{2-}$. As the concentration of background electrolytes increased, Cr(VI) adsorption decreased. This result mightly suggests that adsorption between the surface of RPW and Cr(VI) occurs through outer-sphere complex. Cr(VI) adsorption onto the RPW was well described by second-order kinetics. From the Langmuir isotherm at initial pH 3, the maximum adsorbed amount of Cr(VI) onto the RPW was 11.1, 10, 3.3, 5 mg/g in the presence of $NO_3\;^-,\;CO_3\;^{2-},\;SO_4\;^{2-}$, and $PO_4\;^{3-}$, respectively.

Characteristic Assessment of Heavy Metals in Dusts Collected by the Air Filtration System at Subway Stations in Daegu, Korea (대구지역 지하철역사 공기여과필터 포집먼지에 함유된 중금속성분의 특성평가)

  • Do, Hwa-Seok;Song, Hee-Bong;Shin, Dong-Chan;Kwak, Jin-Hee;Lee, Myoung-Sook;Yoon, Ho-Suk;Kang, Hye-Jung;Phee, Young-Gyu
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.1
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    • pp.42-50
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    • 2009
  • Samples of subway dust were collected by the air filter system of 30 subway stations on Daegu subway line 1 in January 2008. Samples were sieved below 100 ${\mu}m$, and 14 elements were analyzed using ICP after acid extraction. Results obtained from the source assessment of trace elements using enrichment factor showed that Ca, Fe, K, Mg, Mn, Na, V were influenced by natural sources such as weathered rock and resuspended soil, while Cd, Cr, Cu, Ni, Pb and Zn were influenced by anthropogenic sources such as fuel combustion and waste incineration. Concentrations were remarkably higher in components from natural sources than in components from anthropogenic sources. Anthropogenic sources were significantly affected by indoor dusts than outdoor dusts. Results of pollution indices of heavy metals indicated that indoor dusts were more contaminated with heavy metal ions than outdoor dusts. The correlation analysis among trace elements indicated that components were much correlated in the order of natural sources-anthropogenic sources, anthropogenic sources-anthropogenic sources, natural sources-natural sources in both indoor and outdoor dusts. Trace element components of outdoor dusts were largely correlated than those of indoor dusts. In addition, indoor dusts were significantly affected by outdoor dusts rather than depth from the surface or the average daily number of subway passengers.

Optimum Double-Row Spacing in the Autumn Cultivation of Radish (Raphanus sativus L.) (가을 무(Raphanus sativus L.)두 줄 재배를 위한 적정 재식거리)

  • Kang, Eun Seon;Ha, Sun Mi;Cheong, Seoung Ryong;Seo, Myeong Whoon;Park, Su hyoung;Kwack, Yong-Bum;Choi, Keun Jin;Chae, Won Byoung
    • Korean Journal of Environmental Agriculture
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    • v.34 no.3
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    • pp.204-209
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    • 2015
  • BACKGROUND: Radish (Raphanus sativus L.) is cultivated worldwide and one of important vegetables in Korea where year-round production of radish is possible. Most of radishes in autumn are cultivated with double-row spacing except for Gangwon-do where sing-row spacing is predominant. However, no research has been conducted on double-row spacing in radish cultivation so far. This study was conducted to reveal the optimum double-row spacing in autumn cultivation of radish. METHODS AND RESULTS: Using top two popular autumn radish cultivars 'S' and 'C', seeds were sown in spacing between rows of $55{\times}25$, $45{\times}25$, $35{\times}25$ and $25{\times}25cm$, and that within rows of $35{\times}28$, $35{\times}25$, $35{\times}22$ and $35{\times}19cm$. Plants were harvested 58 days after sowing and leaf weight, length and number, and root weight, length sugar content and pithiness were investigated. In the spacing between rows, no significant difference was observed in leaf weight, length and number in both cultivars; however, 25 cm of spacing between rows significantly reduced the root length and weight in 'S' and 'C' cultivars, respectively. In spacing within rows, 28, 25 and 22 cm did not affect fresh root weight in both cultivars, producing appropriate radish roots of 1,500g on average. However, 19 cm of spacing within rows did not reduced fresh root weight in 'S' cultivar but did significantly in 'C' cultivar (1148.3 g). Sugar contents and pithiness of roots were also affected by spacing but its effect was very small and different between cultivars. CONCLUSION: It is suggested that optimum double-row spacing in autumn radish cultivation is 35 cm and 22 cm of spacing between and within rows, respectively, the spacing that did not reduce the yield and quality of radish roots in two popular autumn radish cultivars.

Behavior and Decomposition Velocity of Pollutants on Various Forms from Domestic Sewage in Small-scale Sewage Treatment Plant by Natural Purification Method (자연정화공법에 의한 농촌 전원독립가구 하수처리장에서 하수 중 오염물질의 존재형태별 거동과 분해속도)

  • Seo, Dong-Cheol;Kim, Hyung-Jun;Park, Woo-Young;Lim, Jong-Sir;Hwang, Seung-Ha;Park, Chan-Hoon;Choi, Jeong-Hwan;Lee, Hong-Jae;Lee, Do-Jin;Cho, Ju-Sik;Heo, Jong-Soo
    • Korean Journal of Environmental Agriculture
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    • v.27 no.1
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    • pp.18-26
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    • 2008
  • Behavior and decomposition velocity of pollutants on various forms from domestic sewage in sewage treatment plant were investigated in order to obtain the basic data for improving the removal efficiency of pollutants and to reduce the area in constructed wetland by natural purification method. The removal amounts of BODs and CODs in aerobic bed were significantly higher than those of the other beds. In aerobic bed, the removal amounts of IBOD and ICOD were more than those for SBOD and SCOD, respectively, whereas the removal amounts of BODs and CODs in anoxic and anaerobic beds were little different. The removal amounts of SSs in aerobic bed were also higher than those for the other beds, and the removal amounts of VSS in all beds were more than those for FSS. The removal amounts of DTN and DTP in all beds were more than those for STN and STP, respectively. In addition, the decomposition velocities of TBOD, TCOD and TSS in aerobic bed were 30.79, 17.15 and 29.96 $day^{-1}$. Moreover, the decomposition velocities of BODs, CODs and SSs in aerobic bed were very rapid than those in the other beds. On the other hand, the decomposition velocities of BODs, CODs and SSs in anoxic and anaerobic beds were a little different regardless of the forms of pollutant. The decomposition velocities constants of T-N in aerobic, anoxic and anaerobic beds were 4.78, 0.12 and 0.10 $day^{-1}$, respectively. Moreover, the decomposition velocities constants of T-P in aerobic, anoxic and anaerobic beds were 13.09, 0.12 and 0.13 $day^{-1}$ respectively. The decomposition velocity of T-Ns and T-Ps in aerobic bed were slightly rapid than those in the other beds, whereas the decomposition velocities of T-Ns and T-Ps in anoxic and anaerobic beds were slightly different regardless of the forms of pollutant.

A Study on the Improvement of Treatment Efficiency for Nitrogen and Phosphorus by Improved Sewage Treatment Process in Constructed Wetland by Natural Purification Method (자연정화공법에 의한 인공습지 하수처리장에서 하수처리 공정개선에 따른 질소 및 인의 처리효율 향상 방안)

  • Seo, Dong-Cheol;Park, Woo-Young;Lim, Jong-Sir;Park, Chan-Hoon;Lee, Hong-Jae;Kim, Hong-Chul;Lee, Sang-Won;Lee, Do-Jin;Cho, Ju-Sik;Heo, Jong-Soo
    • Korean Journal of Environmental Agriculture
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    • v.27 no.1
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    • pp.27-34
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    • 2008
  • To effectively treat the domestic sewage that was produced on a small-scale in farming and fishing village in order to encourage an ecologically friendly environment, a small-scale sewage treatment apparatus using natural purification methods that consisted of an aerobic and an anaerobic plots were constructed. The efficiency of sewage treatment according to the sewage loading was investigated to obtain the optimum sewage loading in small-scale sewage treatment apparatus. Removal rate of pollutants according to the sewage loading were in the order of $150\;Lm^{-2}day^{-1}{\fallingdotseq}300\;Lm^{-2}day^{-1}>600\;Lm^{-2}day^{-1}$. Therefore, the optimum sewage loading was 300 L m-2 day-1. Under the optimum sewage loading, removal rate of BOD, $COD_{Mn}$, turbidity, T-N and T-P were 99, 94, 99, 49 and 89%, respectively. However, to satisfy the water quality standard in effluent in small-sclae sewage treatment apparatus for domestic sewage treatment, the low removal efficiency of T-N and T-P must be improved. So to improve the removal rate of T-N and T-P, the efficiency of sewage treatment according to the improved sewage treatment process such as, re-treatment at aerobic plot, anaerobic condition of aerobic plot, changing the filter media sizes and the depths in anaerobic plot, and also addition of oyster shells to filter media at anaerobic plot were investigated. In case of 150 cm depth in anaerobic plot with filter medium A (effectivity particle size 1.50 mm) and addition of oyster shells to filter media at anaerobic plot, removal rate of T-N and T-P in both plots were increased by 10 and 3%, and 14 and 7% in comparison with 100 cm depth in anaerobic plot with filter medium B(effectivity particle size 0.95 mm), respectively. The optimum improved sewage treatment process in small-scale sewage treatment apparatus were 150 cm depth in anaerobic plot with filter medium A and addition of oyster shells to filter media at anaerobic plot.

A Study on the Development of a Microbial Insecticide -(With special emphasis on formulation)- (미생물(微生物) 살충제(殺蟲劑)의 개발(開發)에 관(關)한 연구(硏究) -(제제화(製劑化)를 중심(中心)으로)-)

  • Lee, Jae-Koo;Kim, Ki-Cheol;Kim, Do-Young
    • Applied Biological Chemistry
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    • v.22 no.2
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    • pp.123-134
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    • 1979
  • For the purpose of developing a microbial insecticide utilizing Bacillus thuringiensis Berliner, research was done and the following results were obtained. 1) As the freeze-dried matter of the cocoon-cooked water discarded from the filature contains much crude protein(51.825%) and a lot of inorganic salts, it can make a good nutrition source for the culture cf B. thuringiensis Berliner. 2) Based on the suspensibility, formula F-5 turned out to be the most suitable for insecticidal use. Its composition includes 0.2 g of the cell-spore-crystal mixture, 25 g of 200-mesh kaolin, 2.5 g of New Kalgen-NX-150, and 2.5 g of glycerine admixed with 8 ml of distilled water and granulated in 80-mesh size. 3) All the components of F-5, F-6 and F-7 are identical except that the amounts of cell-spore-crystal mixture of F-5, F-6, and F-7 are 0.2 g, 0.4 g, and 0.6 g, respectively. Accordingly, their physical properties are almost all the same. 4) Formulas F-5, F-6, and F-7 exhibited an excellent toxicity to Anomis mesogona Walker, Dendrolimus spectabilis Butler, and Margaronia perspectalis Walker at the concentration of 5%. 5) Formulas F-8 and F-9 which contain $NaHCO_3$ as one of their components showed a remarkably reduced toxicity to Anomis mesogona Walker and Dendrolimus spectabilis Butler than F-6 which does not contain $NaHCO_3$. 6) A maximum of $2.97{\times}10^9$ spores per ml was obtained by incubating B. thuringiensis in M-3 which has a pH of 7.05 and comprises 0.2% of ammonium sulphate and 0.8% of glucose dissolved in the cocoon-cooked water, with aeration for 96 hours. 7) Formula F-6 exhibited a somewhat reduced toxicity to Anomis mesogona Walker and Dendrolimus spectabilis Butler, when stored at room temperature for 70 days after formulation and it is desirable to keep it in a dark and cold place. 8) In held applications, formula F-6 showed a good activity in controlling Monema flavescens Walker. Margaronia perspectalis Walker, and Macrosiphum ibarae Matsumura.

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Effects of Oyster-Shell Meal on Improving Spring Chinese Cabbage Productivity and Soil Properties (굴 패화석 비료가 배추 수량 및 토양 특성에 미치는 효과)

  • Lee, Ju-Young;Lee, Chang-Hoon;Yoon, Young-Sang;Ha, Byung-Hyun;Jang, Byoung-Choon;Lee, Ki-Sang;Lee, Do-Kyoung;Kim, Pil-Joo
    • Korean Journal of Soil Science and Fertilizer
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    • v.38 no.5
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    • pp.274-280
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    • 2005
  • Enormous amount of oyster-shell waste has been illegally disposed at oyster farm sites along the southern coast of Korea and then made a serious problem in environmental side. To increase the consumption of oyster shell meal as a soil amendment, the effects of oyster shell meal on soil properties and spring Chinese cabbage productivity were evaluated in silt loam soil to which 0, 4, 8, 12 and $16Mg\;ha^{-1}$ of oyster-shell meal fertilizer were added. Hydrated lime treatment ($2Mg\;ha^{-1}$) was selected as a control. Oyster-shell meal fertilizer, which made by a simple crushing and has high alkalinity as a calcium materials, had significant effects on neutralizing acid soil and on supplying calcium element. Spring Chinese cabbage yields increased with shell meal application. Plant uptakes of macro-nutrients such as P and C, and micro-nutrient such as B were significantly increased by oyster-shell meal application and then contributed to promote Chinese cabbage growth. The highest yield was achieved following the addition of $8Mg\;ha^{-1}$ shell meal application, and the same yield with that in lime treatment was at the $4Mg\;ha^{-1}$. Oyster-shell meal had more substantial effect on suppling calcium and on improving soil pH than that of lime. In conclusion, oyster shell meal fertilizer could be a good supplement to other inorganic soil amendments to improve nutrient balances in upland soils.

Effects of Liquid Pig Manure Application on Rice Growth and Environment of Paddy Soil (돈분뇨 액비 시용이 벼의 생육 및 논 토양 환경에 미치는 영향)

  • Jeon, Weon-Tai;Park, Hyang-Mi;Park, Chang-Yeong;Park, Ki-Do;Cho, Young-Son;Yun, Eul-Soo;Kang, Ui-Gum
    • Korean Journal of Soil Science and Fertilizer
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    • v.36 no.5
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    • pp.333-343
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    • 2003
  • This experiment was carried out to improve the utilization of liquid pig manure (LPM) for rice at the two textures of valley soil in 2000 and 2001. The soil textures were coarse loamy and fine loamy in Sachon and Jisan series, respectively. Treatments consisted of no fertilized plot, chemical fertilized plot, LPM 150%, LPM 100%, LPM 100%+NK (top dressing) 30%, LPM 70%+NK 30%, LPM 50%+NK 50% plot. LPM was applied as basal fertilizer compare to nitrogen of chemical fertilized plot. Total N contents in the LPM were 6.0 and $4.5g\;kg^{-1}$ in 2000 and 2001, respectively. After the experiment, P and K contents of soils were not difference between chemical and LPM application plots. But heavy metal contents in soils were slightly higher in LPM application plots than in chemical fertilized plot. Immediately after LPM application, ammonia gas content was $18mg\;kg^{-1}$ in LPM 150% plot, but it was $3mg\;kg^{-1}$ in LPM 50% plot. Two days after LPM application, ammonia gas content was 3 times higher in coarse loamy than in fine loamy soil. After rotary tillage, ammonia gas was not detected at all LPM treatments. This result suggests that rotary tillage can reduce the nasty smell of LPM quickly. Inorganic nitrogen, $NO_3$ and $NH_4$, contents in water of paddy was higher at coarse loamy soil from rice transplanting to tillering stage. After that season, inorganic nitrogen contents of water were not different according to soil texture and treatments. Content of $NH_4-N$ in soil solution was higher at LPM plots than chemical fertilizer plot. Total nitrogen contents in rice plant after harvesting were higher at chemical fertilization plot than LPM application plot, but K contents showed an opposite tendency. Rice yield was decreased only in LPM plots at two soil textures. But yield was not significantly difference between chemical fertilizer and LPM+top dressing plots at coarse loamy soil and increased 5% at LPM 50%+NK 50% plot at fine loamy soil in 2001.

Smad6 Gene and Suppression of Radiation-Induced Apoptosis by Genistein in K562 Cells (K562 세포주에서 Genistein에 의해 억제되는 Radiation-induced Apoptosis의 조절 유전자)

  • Jeong, Soo-Jin;Jin, Young-Hee;Yoo, Yeo-Jin;Do, Chang-Ho;Jeong, Min-Ho;Huh, Gi-Yeong;Bae, Hye-Ran;Yang, Kwang-Mo;Moon, Chang-Woo;Oh, Sin-Geun;Hur, Won-Joo;Lee, Hyung-Sik
    • Radiation Oncology Journal
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    • v.19 no.3
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    • pp.245-251
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    • 2001
  • Prupose : The genes involved on the suppression or radiation-induced apoptosis by genistein in K562 leukemia cell line was investigated. Materials and methods : K562 cells in exponential growth phase were irradiated with a linear accelerator at room temperature. For X-ray irradiation and drug treatment, cultures were prepared at $2\times10^5\;cells/mL$. The cells were irradiated with 10 Gy (Clinac 1800C, Varian, USA), Stock solutions of herbimycin A (HMA, Calbiochem, UK) and genistein (Calbiochem, UK) were prepared in dimethylsulfoxide (DMSO, Sigma, UK). After incubation at $37^{\circ}C$ for 24 h, PCR-select cDNA subtractive hybridization, dot hybridization, DNA sequencing and Northern hybridization were examined. Results : Smad6 gene was identified from the differentially expressed genes in K562 cells incubated with genistein which had been selected by PCR-select cDNA subtractive hybridization. The mRNA expression of Smad6 in K562 cells incubated with genistein was also higher than control group by Northern hybridization analysis. Conclusion : We have shown that Smad6 involved on the suppression of radiation-induced apoptosis by genistein in K562 leukemia cell line. It is plausible that the relationship between Smad6 and the suppression of radiation-induced apoptosis is essential for treatment development based on molecular targeting designed to modify radiation-induced apoptosis.

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