• Title/Summary/Keyword: DO (Dissolved Oxygen)

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Application of Scale-Up Criterion of Constant Oxygen Mass Transfer Coefficient ($k_La$) for Production of Itaconic Acid in a 50 L Pilot-Scale Fermentor by Fungal Cells of Aspergillus terreus

  • Shin, Woo-Shik;Lee, Dohoon;Kim, Sangyong;Jeong, Yong-Seob;Chun, Gie-Taek
    • Journal of Microbiology and Biotechnology
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    • v.23 no.10
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    • pp.1445-1453
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    • 2013
  • The scale-up criterion of constant oxygen mass transfer coefficient ($k_La$) was applied for the production of itaconic acid (IA) in a 50 L pilot-scale fermentor by the fungal cells of Aspergillus terreus. Various operating conditions were examined to collect as many $k_La$ data as possible by adjusting the stirring speed and aeration rate in both 5 L and 50 L fermentor systems. In the fermentations performed with the 5 L fermentor, the highest IA production was obtained under the operating conditions of 200 rpm and 1.5 vvm. Accordingly, we intended to find out parallel agitation and aeration rates in the 50 L fermentor system, under which the $k_La$ value measured was almost identical to that ($0.02sec^{-1}$) of the 5 L system. The conditions of 180 rpm and 0.5 vvm in the 50 L system turned out to be optimal for providing almost the same volumetric amount of dissolved oxygen (DO) into the fermentor, without causing shear damage to the producing cells due to excessive agitation. Practically identical fermentation physiologies were observed in both fermentations performed under those respective operating conditions, as demonstrated by nearly the same values of volumetric ($Q_p$) and specific ($q_p$) IA production rates, IA production yield ($Y_{p/s}$), and specific growth rate (${\mu}$). Specifically, the negligible difference of the specific growth rate (${\mu}$) between the two cultures (i.e., $0.029h^{-1}$ vs. $0.031h^{-1}$) was notable, considering the fact that ${\mu}$ normally has a significant influence on $q_p$ in the biosynthesis of secondary metabolites such as itaconic acid.

Nutrient dynamics study of overlying water affected by peroxide-treated sediment

  • Haque, Niamul;Kwon, Sung-Hyun
    • Journal of Ecology and Environment
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    • v.41 no.9
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    • pp.235-245
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    • 2017
  • Background: Loading of excess nutrient via bioremediation of polluted sediment to overlying water could trigger anoxia and eutrophication in coastal area. The aim of this research was to understand the changes of overlying water features such as dissolved oxygen (DO); pH; oxidation reduction potential (ORP); $chlorophyll-{\alpha}$ ($Chl-{\alpha}$); and nitrogen nutrients ammonia ($N-NH_4{^+}$), nitrate ($N-NO_3{^-}$), and nitrite ($N-NO_2^-$) when the sediment was not treated (control) and treated by calcium peroxide for 5 weeks. Methods: The water samples were analyzed for measuring physical and chemical properties along with the sediment analyzed by polymerase chain reaction (PCR) including denaturing gradient gel electrophoresis (DGGE) for identifying the phylogenetic affiliation of microbial communities. Results: Results showed that due to the addition of calcium peroxide in sediment, the overlying water exposed the rise of dissolve oxygen, pH, and ORP than control. Among the nitrogen nutrients, ammonia inhibition was higher in calcium peroxide treatment than control but in case of nitrate inhibition, it was reversed than control. $Chlorophyll-{\alpha}$ was declined in treatment column water by 30% where it was 20% in control column water. Actibacter and Salegentibacter group were detectable in the calcium-peroxide-treated sediment; in contrary, no detectable community ware found in control sediment. Both phylogenetic groups are closely related to marine microflora. Conclusions: This study emphasizes the importance of calcium peroxide as an oxygen release material. Interaction with peroxide proved to be enhancing the formation of microbial community that are beneficial for biodegradation and spontaneity of nutrient attenuation into overlying water.

The Characteristics os Water Quality of Tap water and Far-infrared rays mineral water (수돗물과 원적외선 기능수의 수질 특성의 비교)

  • 백우현
    • Journal of Environmental Science International
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    • v.9 no.5
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    • pp.423-428
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    • 2000
  • The characteristics of Far-infrared rays mineral water(FIR water) have been compared to the tap water by means of relationship between FIR water and Nuclear Magnetic Resonance spectroscopy(NMR), FIR water and thermography FIR water and velocity of blood FIR-water and pH, FIR water and dissolved oxygen(DO), FIR water and Oxidation-Reduction Potential(ORP) using the development FIR water purification of grand prix system. From the experimental result are quite satisfactory when compared with the tap water. Also the FIR water were evaluated to see if those are tasty and healthy using the Hashimoto's Mineral Balance Index. As a result FIR-water was found as tasty and healthy.

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Development of Automatic Nutrient-Solution Controller Using Single-chip Microcomputer (원칩 마이크로 컴퓨터를 이용한 양액 자동 조제 장치의 개발)

  • 오길근;류관희;홍순호;김효중
    • Journal of Biosystems Engineering
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    • v.20 no.4
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    • pp.383-389
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    • 1995
  • This study was conducted to develop an automatic nutrient control system for trickle application of nutrient solution. Temperature, electric conductivity(EC). pH and dissolved oxygen(DO) were selected as control variables. A controller using single-chip microcomputer was constructed. An automatic control system for nutrient solution and a controller using single-chip microcomputer with control algorithm were developed. The control system was tested, and could control temperature, EC and pH within the error ranges of $pm 0.2^{circ} pm 0.2mS/cm, pm 0.1pH$, respectively.

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Factors Influencing Nitrite Build-up Nitrification of High Strength Ammonia Wastewater (고농도 암모니아성 폐수의 질산화과정에서 아질산염 축적의 영향인자)

  • 한동준;강성환;임재명
    • Journal of environmental and Sanitary engineering
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    • v.13 no.2
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    • pp.128-138
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    • 1998
  • This study was focused to find how each factors effect on the biological nitrification in wastewater treatment under high ammonia nitrogen concentration. Batch reactors in aerobic conditions were used to test the treatment efficiency of mixed liquor, nightsoil and piggery wastewater. The results are summeried as follows; Initial ammonia nitrogen concentration and pH were the direct influencing factors of nitrite build-up. More than 250 mg NH$_{4}$$^{+}$ - N/L in initial concentration built up nitrite and then the inhibition rate to Nitrobacter was above 70 percentage. And maximum nitritation rate was showed at pH 8.3 and nitrification could be completely achieved by pH control. Temperature and dissolved oxygen were the indirect influencing factors of nitrite build-up. These were a great effect on the activity of nitrifying microbes and ammonia nitrogen removal. Maximum nitritation rate was showed at 30 $\circ $C. The effect of DO concentration was negligible at more than 3 mg/L.

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A Study on the Variation of Nutrients and Water Quality in Suk Chon Lake (石村湖의 營養鹽類 및 수질의 動態에 관한 조사연구)

  • Choi, Han-Young;Kim, Tai-Jeon;Park, Sung-Bae
    • Journal of Environmental Health Sciences
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    • v.14 no.1
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    • pp.47-53
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    • 1988
  • This study was performed to investigate a seasonal and vertical variation of the nutrients, water temperature, pH, DO, BOD, COD in Sukchon lake from May 1985 to Jan. 1986. The results were as follows. 1. Vertical descent of water temperature was the remarkable situation on Jul. and difference between surface layer and bottom layer was 3.5$\circ$C. 2. The value of pH was average 8.0 in both lake. The value of pH in the winter was average 7.6, and Summer was average 8.3. 3. The contents of dissolved oxygen was the highest value (13.0mg/l in surface layer on Jan. and the lowest value (8.1mg/l in bottom layer on Ul. 4. The contents of PO$_4$-P, NH$_3$-N, NO$_3$-N and T-N were 0.03~O.17mg/l 0.12~0.34mg/l 0.36~0.91mg/l and 0.76~1.37mg/l respectively.

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A New Species of the Genus Cavernocypris Hartmann, 1964 (Crustacea, Ostracoda) from Gosu cave in Korea

  • Kim, Byung-Woo;Kwon, Jung-Kyun;Lee, Won-Cheol
    • 한국전자현미경학회:학술대회논문집
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    • 2004.11a
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    • pp.105-110
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    • 2004
  • Cavernocypris gosuensis n. sp. (Cyprididiae, Cypridopsinae), a new subterranean ostracod species is described with figures of female valves, mouthparts, appendeges and SEM photos from Gosu cave, Danyang-Gun, Chungcheongbuk-Do in Korea. The new stygobiont speices is sixth of the genus Caveroncypris Hartmann, 1964 that characterized by both valeves with a median concave part in ventral view and two groove lines in dorsal view, the reduction of the furca, which is flagellum like in female and the left valve ventrally overlaps the right valve when the carapace is closed and can be distinguished from its congers by left valve with 6 muscle scars in innerpart, mandible palp with one claw and 3 setae on the terminal segment and without seta on the exterodistal border of segment III. The animals were collected within pH 7.31-8.74, temperature ($^{\circ}C$) 13.0-14.6 and dissolved oxygen (mg/l 6.75-14.84 from Aug.2003 to Sep.2004.

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해수-석탄회 상호작용에 의한 원소 용출특성: 실험연구

  • 박성민;김강주;장수범;황갑수
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2003.04a
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    • pp.455-458
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    • 2003
  • We investigated the leaching of elements from anthracite ashes by interactions with seawater. The results show that consumption rates of dissolved oxygen are 7.5 times greater in the seawater system than in the fresh water system and indicate that the differences in DO consumption rate may plat a role in regulating the element leaching from the coal ashes. It is revealed that seawater's pH buffering capacity is the most important factor that makes the leaching of elements and their chemical behaviors in the seawater system different from those in the fresh water environments. In overall, element leaching from the weathered ash is smaller than that from the fresh ash. However, the leaching of Si, Fe, Al, Mn, phosphate, and some other elements were independent of weathering. They were dependant only on the pH of the solutions.

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Runoff characteristics of organic matters and nutrients from greenhouse site in paddy field area (논 지역 시설재배지에서의 유기물 및 영양염류 유출 특성)

  • Lee, Keun-Hoo;Ok, Jeong-Hoon;Ryu, Si-Chang;Yu, Chan
    • Proceedings of the Korean Society of Agricultural Engineers Conference
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    • 2005.10a
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    • pp.604-609
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    • 2005
  • Runoff characteristics of organic matters and nutrients from greenhouse site in paddy field area were investigated during the irrigation period in 2004. The greenhouse selected which situated near the Nam river, Jinju, Korea used the ground water as the irrigation purpose. And, the rotating system of paddy field to upland was adapted as a cropping system which is a typical practice in this area. Various items such as total phosphorus(T-P), total nitrogen(T-N), dissolved oxygen(DO), BOD, etc. were observed to figure out the seasonal and spatial variation in the study sites. it was found that the risk of water pollution due to the contamination of nutrients in canals in green house sites, and their neighbouring small sized streams are much higher than those in ordinary paddy field areas. Further surveys and investigations are required to find out the counter measures to reduce water pollution occurred in greenhouse sites.

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The Treatment of Acid Mine Drainage - The removal of Iron(Fe) component- (자력에 의한 산성 광산 배수의 처리 - 철(Fe) 성분의 제거-)

  • Song, Kun-Ho;Lee, Kwang-Rae
    • Journal of Industrial Technology
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    • v.32 no.A
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    • pp.21-27
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    • 2012
  • The characteristics of floc formation of the iron(Fe) ions contained in the acid mine drainage was studied for developing the process treating the acid mine drainage. The iron(Fe) ions were formed into flocs by the acid-base reaction with the added $Ca(OH)_2$. The molal ratio of iron(Fe) vs $Ca(OH)_2$ was one of major control variables in treatment; pH change, iron(Fe) ions concentration in treated drainage, DO (dissolved oxygen content). In addition, the air gave much effect on the color of the $iron(Fe)-Ca(OH)_2$ flocs and the attachment to magnet. The attaching to the magnet of the flocs formed in the air was much less than the case without air.

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