• 한국잠사곤충학회지
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• 제51권1호
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• pp.63-67
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• 2013

8개 연구기관의 지역적응 시험을 통해 황보잠은 기존 보급 누에품종인 금황잠보다 화용비율이 높은 강건성 품종으로 대조 품종대비 5령 3일 유충 무게가 3%정도 무겁고, 전견중이 무거워 수량성이 높은 다수성 양친한성황견 품종으로 육성하게 되었다. 이 품종의 일본종계 원종 잠317는 1994년에 Y1, 8013의 교잡으로 2011년에 육성기호 Y9411으로 명명하였다. 암수 모두 무늬가 있는 형잠 품종으로 고치모양은 땅콩형이며 암수고치색깔이 다른 한성황견 품종이다. 중국종계 잠318은 1994년에 Y1, 9134 의 교잡으로 2004년에 육성기호 Y9416으로 명명하였다. 암수 모두 무늬가 없는 희잠 품종으로 고치모양은 타원형이며 암수고치색깔이 다른 한성황견 품종이다. 황보잠은 춘잠기에 적합한 품종으로 사육성적에서 화용 비율이 93.2%, 1만두 수견량이 21.3 kg, 견층중이 54.8 cg으로 대조 품종 금황잠보다 우수하였으며, 또한 실켜기 성적에서 해서율, 생사량이 80%, 4.65kg로 대조 78%, 4.05 kg보다 우수하였다. DNJ(1-Deoxynojirimycin)함량은 황보잠이 대조 금황잠보다 낮았고, 누에 동충하초 생산성에서 감염율은 대조보다 3,9%로 높았고 생체중은 개당 1.32 g으로 대조 1.19 g 보다 무거웠다.

• 한국잠사곤충학회지
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• 제48권2호
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• pp.37-40
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• 2006

다수성 품종으로 육성된 대풍잠은 새로운 봄누에 품종으로서 육성경위와 주요특성을 요약하면 다음과 같다. 1. 일본종계 원종 잠151은 도입 육성한 무늬 품종이며, 중국종계 원종 잠152는 교잡 육성한 강건다수성 한성무늬 누에품종이다. 2. 2005년 춘잠기 지역적응시험 결과는 대풍잠이 다수견, 다사량의 특성을 지닌 금옥 잠에 비해 전견중이 무거워 고치수량이 6% 증수되었다. 3. DNJ 함량은 대조 금옥잠 4.26 mg과 같은 수준인 4.22 mg으로 함량이 우수한 편이었고, 동충하초 생산력 검정에서 동충하초 생체중이 1개당 1.61 g으로 대조 1.41 g 보다 14% 무거워 다수성 특성을 나타냈다. 4. 조사성적에서는, 견사장은 1,536 m로 대조대비 지수로 8%가 떨어지나, 섬도가 굵고 견사량이 많아 1만두 생사량이 5.24 kg/만두로 대조 대비 지수로 4% 높아 다사량의 특성을 나타내었다. 5. 잠종생산능력에 있어서는 일본종계 원종 잠151의 나방당 산란수는 564개로 대조와 비슷하였다. 중국종계 원종 잠152의 산란수는 588개로 일반장려품종의 원종에 비해 산란성이 우수하였다.

• 동아시아식생활학회지
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• 제23권6호
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• pp.768-777
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• 2013

Physicochemical characteristics and analysis of functional constituents of mulberry (Morus alba L.) fruit juices were investigated according to four different mulberry cultivars, including iksuppong, daeseongppong, cheongilppong and kwasangppong. Among the four mulberry cultivars examined, the small-sized cheongilppong had higher soluble solid content and lower titratable acidity, whereas the big-sized daeseongppong had higher titratable acidity and lower soluble solid content than other mulberry cultivars. Cheongilppong had higher contents of fructose and glucose, while daeseongppong had higher contents of citric and malic acids than the other mulberry cultivars. Kwasangppong had higher contents of two anthocyanins, cyanidin 3-glucoside and cyanidin 3-rutinoside, while cheongilppong had lower contents of two anthocyanins than the other mulberry cultivars. Daeseongppong had higher contents of resveratrols, flavonoids and moracin, whereas cheongilppong had higher levels of protocatechuic, chlorogenic and caffeic acids than the other mulberry fruits. Of the four mulberry cultivars, daeseongppong and cheongilppong had higher contents of GABA and DNJ, respectively, than the other mulberry cultivars. These results provide useful information to food technologists for the development and standardization of high quality mulberry juices as well as their processed foods.

• International Journal of Industrial Entomology and Biomaterials
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• 제21권2호
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• pp.163-167
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• 2010

[ $\alpha$ ]Glucosidase (EC 3.2.1.20) is a glycosidase that hydrolyzes disaccharides, oligosaccharides, and polysaccharides resulting in the release of α-D-glucose. In this study, $\alpha$-glucosidase activity in the hemolymph and midgut of the mulberry silkworm Bombyx mori and Japanese oak silkmoth Antheraea yamamai was measured using maltose, sucrose, trehalose, and p-nitrophenyl $\alpha$-D-glucopyranoside as substrates. In general, hemolymph $\alpha$-glucosidase activity was higher in B. mori than in A. yamamai. In contrast, midgut $\alpha$-glucosidase activity was higher in A. yamamai than in B. mori for all of the substrates tested. $\alpha$-Glucosidase activity in the midgut of both B. mori and A. yamamai showed similar responses to changes in pH and temperature for all of the substrates tested. Native (7.5%) PAGE of hemolymph and midgut proteins from B. mori and A. yamamai followed by staining with 4-methylumbelliferyl $\alpha$-D-glucoside (MUG) indicated that the $\alpha$-glucosidases of these related lepidopterans are functionally similar but structurally different. In comparison to $\alpha$-glucosidase activity from A. yamamai, $\alpha$-glucosidase activity from B. mori was generally less sensitive to the $\alpha$-glucosidase inhibitors, 1-deoxynojirimycin (DNJ), acarbose, and voglibose when the activity was determined using maltose, sucrose, and trehalose.

• 한국약용작물학회지
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• 제20권6호
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• pp.472-478
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• 2012

This study was carried out to investigate solid-state fermentation method using cellulolytic lactic acid bacteria Lactobacillus plantarum TO-2100 in order to increase piperidine alkaloid contents in mulberry leaves. Piperidine alkaloid, one type of which include 1-deoxynojirimycin (1-DNJ), is known to inhibit ${\alpha}$-glycosidase activities. Using this strain, the optimal solid-state fermentation conditions on mulberry leaves powder were found as the following: initial moisture content, temperature and relative humidity were 20%, $30{\sim}35^{\circ}C$ and 60 ~ 70%, respectively, and the fermentation time was 72 hrs. The piperidine alkaloid contents in the fermented mulberry leaves were 2.86% on dry powder, which is 7-fold increase from that of non-fermented mulberry leaves. The 1-deoxynojirimycin contents after applying preparative thin layer chromatography were 2.02% on dry powder, which is 8 times higher than that of non-fermented mulberry leaves. ${\alpha}$-Glycosidase activities was inhibited by 65.7 ~ 84.7% with 3 ~ 5% treatments of hot-water extracts of the fermented mulberry leaves, compared to 16.2 ~ 40.2% with 3 ~ 5% treatments of hot-water extracts of non-fermented mulberry leaves. Therefore, the results suggest that solid-state fermentation method does indeed increase of piperidine alkaloid contents on mulberry leaves.

• International Journal of Industrial Entomology and Biomaterials
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• 제48권3호
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• pp.156-162
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• 2024

This study aimed to evaluate and compare the inhibitory effects of extracts from Bombyx batryticatus (BBE) and Bombyx mori (BME) on α-glucosidase, DPP-4, and LDL oxidation activities, focusing on their potential applications in managing postprandial hyperglycemia and metabolic syndrome. The results demonstrated that both BBE and BME effectively inhibited α-glucosidase and LDL oxidation, with BBE exhibiting higher inhibitory activity than BME. HPLC analysis identified linolenic acid, linoleic acid, linolenic acid ethyl ester, pheophorbide a, and pyropheophorbide a as key compounds contributing to these effects. Notably, the identified unsaturated fatty acids and pheophorbides showed strong α-glucosidase inhibitory activity, surpassing that of acarbose, a standard diabetic drug. These results suggest that, in addition to the previously reported 1-DNJ and fibroin proteins, unsaturated fatty acids and chlorophyll-derived pheophorbides may play significant roles in glycemic control. Compounds, particularly those from BBE, present promising opportunities for the development of natural therapeutic agents for diabetes management. The study concludes that BBE and BME have strong potential as functional ingredients in future diabetes treatment strategies, possibly offering enhanced efficacy over conventional inhibitors.

• International Journal of Industrial Entomology and Biomaterials
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• 제40권1호
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• pp.22-27
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• 2020

Silkworm powder was prepared from 3^rd day of 5^th instar silkworm through various drying technology including freeze drying, hot wind drying, infrared drying, and microwave drying. The shape of silkworm dried was different with the drying methods. Freeze drying and microwave drying silkworm looked its original form, but hot wind drying and infrared drying silkworm looked shriveled and crumpled. The color of silkworm powder freeze-dried changed from yellowish green to hazel with lowering freezing temperature. Heavy metals including Pb, Cd, As, and Hg were lower the food criteria. The results of 1-deoxynojirimycin analysis was shown that BaekOkJam and GoldenSilk silkworm powder was satisfied the criteria of functional food, but YeonNokJam silkworm powder was lower than the criteria. The amino acid composition of silkworm was similar regardless of the frozen temperature in freeze drying process.

• Nutritional Sciences
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• 제6권4호
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• pp.216-222
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• 2003

This study investigated the effects of feeding mulberry leaf extracts on lipid composition in rats fed high cholesterol diets. An initial 30-person sensory evaluation of preparations containing various concentrations of mulberry leaf extract showed that a preparation containing 9% mulberry leaf extracts was the most highly preferred. In addition, subsidiary materials of pine needle extracts and mugwort extracts were added to weaken the unpleasant smell of mulberry leaf extract A preparation containing 9% mulberry leaf extract with 3% mugwort extract and 7% pine needle extract was given highest preference scores by the 30-person panel. When comparing the functional ingredients contents of the various preparations of mulberry leaf extracts, such as GABA, DNJ and flavonoids, no significant differences were found as a result of adding subsidiary materials (pine needle and mugwort extracts). Sprague-Dawley male rats weighing l00$\pm$10g were randomly assigned to one normal diet group, and to four high cholesterol diet groups containing 1% cholesterol, to elucidate the functionality of the mulberry leaf extract The four high cholesterol diet groups were classified into: a mulberry leaf extract diet group free of subsidiary materials (EB group); a mulberry extract diet group with pine needle extracts (EP group); a mulberry leaf extract diet group with mugwort extracts (EM group); and a control group (HC group). The mulberry leaf extracts were provided as drinking water; the diet and water were fed ad libitum. Hepatic cholesterol and triglyceride levels were higher, by 279% to 475%, in the high cholesterol groups compared to the normal diet groups, but were significantly lower in the three groups supplied with mulberry leaf extracts, compared with the high cholesterol control. There were no changes in functionality of the mulberry leaf extract preparations due to the addition of subsidiary materials. In conclusion, preparations of mulberry leaf extracts were shown to improve lipid metabolism in rats fed a high cholesterol diet, by reducing hepatic and plasma triglyceride and cholesterol levels. Also human palatability of the mulberry leaf preparation was improved by adding subsidiary materials such as pine needle and mugwort extracts.

• 한국초지조사료학회지
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• 제29권3호
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• pp.263-268
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• 2009

한우 고급육 생산을 위한 사양관리에 있어 기능성 사료의 활용 가능성을 규명하기 위해 뽕나무 사일리지의 기능성 성분 및 항산화 활성효능을 분석하였다. 뽕나무 사일리지의 일반 성분을 분석한 결과, 건물함량 $28.41{\pm}3.12%$, 조단백질 함량 $12.43{\pm}0.28%$, 조지방 함량 $2.47{\pm}0.18%$, 조섬유 함량 $20.29{\pm}0.75%$ 및 조회분 함량 $6.98{\pm}0.12%$였다. 뽕나무 사일리지에 함유된 활성성분의 분석 결과, 대표적 활성성분으로 혈당강하성분인 1-deoxynojirimycin(1-DNJ)의 함량이 0.568mg/g으로 검량되었으며 혈압강하성분인 GABA($\gamma$-Aminobutyric acid)의 함량은 5,936.22 pmol의 농도가 검출되었다. Flavonoid는 본 연구에서 검출이 되지 않았으며 총 phenol이 21.69${\mu}g/mg$ 정도 함유된 것으로 분석되었다. 뽕나무 사일리지의 DPPH radical 소거활성은 뽕나무 사일리지 추출물의 농도가 높아질수록 비례해서 활성효과가 크게 나타나고 있었으며 0.25mg/ml의 농도에서 50% 이상의 활성효과가 인정되었고, Hydroxyl radical 소거 활성 역시 0.5-2.0mg/ml로 농도가 높아질수록 활성 효과가 커지는 것을 알 수 있었으며 2.0mg/ml의 농도에서는 50% 이상의 활성효과를 나타냈다. Alkyl radical 소거 활성은 보다 낮은 농도에서 높은 활성효과를 나타내었는데 0.125mg/ml의 농도에서 50% 이상의 활성효과를 나타내고 있어 이 역시 높은 항산화 효과가 있음을 알 수 있었다. 이상의 결과를 통해 뽕나무 사일리지의 기능성 가축사료화의 의미를 충분히 가질 수 있을 것으로 평가된다.

• 한국식품영양과학회지
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• 제33권10호
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• pp.1611-1617
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• 2004

본 연구에서 는 뽕잎과 누에가루의 함량 비를 달리 하여 제조한 환의 혈당강하 효과를 in vitro및 동물실험을 통해 검정 하고자 하였다. 실험 동물은 체중 100 g내외 의 Sprague-Dawley종 수컷 흰쥐를 이용하였으며, 제조환은 식이 내에 0.4%(4 ghg)씩 공급하였다. 실험군은 제조환의 종류에 따라 제조환을 공급하지 않은 당뇨 대조군(DM group), 뽕잎 100%로 제조한 군(M group), 뽕잎에 누에가루를 각각 25%(25SM group), 50%(50SM group), 75%(75SM group) 및 100%(100S group)씩 혼합하여 제조한 군으로 나누었다. 식이와 식수는 자유섭식시켰으며,3주 간 사육한 후 STZ로 당뇨를 유발시켰으며, 9일 만에 희생시켰다 제조된 혼합 환의 DNJ의 함량은 누에가루의 혼합 비율이 높을수록 증가되었다. GABA및 rutin의 함량은 뽕잎의 혼합 비율이 증가함에 따라 함유량이 증가되었다. in uitro에서 소장 점막의 $\alpha$-glucosidase의 활성 저해 효과도 뽕잎 에 누에가루를 50% 이상 혼합시켰을 때 혼합하지 않은 환과 2SSM 환보다 유의적으로 증가되었다. 혈당강하 효과는 DM군과 M군에 비해 누에가루를 혼합한 군에서 더 높았으며 특히 50%, 75% 및 100% 혼합한 군들에서는 유의적 인 혈당강하 효과를 나타내었다. 소장 점막 maltase 활성은 proximal 부분에서 DM군에 비하여 제조환 공급군 모두가 유의적으로 감소되었으며, 제조환 공급군 간에는 유의적인 차이는 없었다. Middle부분에서는 실험군 간에 유의적인 차이가 없었다. Distal 부분에서는 DM군에 비하여 제조환 공급군에서 감소되었으며, 특히 50SM군,75SM군 및 100S군에서 현저하게 감소하였다. Sucrase와 lactase의 경우에는 prorimal부분에서 DM군에 비하여 제조환 공급군에서 유의적으로 감소되었으며, middle 및 distal부분에서는 실험군 간에 유의적인 차이가 없었다. 결론적으로 뽕잎과 누에가루를 혼합하여 제조한 환은 in vitro실험 에서 $\alpha$-glucosidase 활성 저해효과를 증가시켰으며, STZ 유발 당뇨 쥐의 소장 proximal 부분의 이 당류 분해 효소 활성을 억제함으로써 급속한 혈당상승을 억제하는 효과가 있었다. 이러한 효과는 뽕잎과 누에가루를 50 : 50의 동량으로 혼합시켰을 때 가장 좋은 것으로 관찰되었다.