• Animal Systematics, Evolution and Diversity
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• 제35권4호
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• pp.182-185
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• 2019

In this study, cytochrome c oxidase subunit I(COI) sequences of 17 individuals from six Korean diogenid species(i.e., 2 Areopaguristes japonicus, 4 A. nigroapiculus, 3 Paguristes digitalis, 4 P. ortmanni, 3 Diogenes edwardsii, and 1 Ciliopagurus kempfi) were determined and analyzed. The DNA barcoding results of this study were consistent with the morphological identification of these six species. Interspecific variations of COI sequences within six Korean diogenid species exceeded the minimum interspecific variation of diogenid hermit crabs in previous studies. Little intraspecific variation exists except for P. digitalis. This study should facilitate further molecular taxonomy of East Asian diogenids.

• Animal Systematics, Evolution and Diversity
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• 제35권3호
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• pp.156-159
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• 2019

The phyllodicd polychaete species, Nereiphylla hera Kato and Mawatari, 1999 is reported from the intertidal habitats of the eastern coast of South Korea. We determined the DNA barcoding region of the mitochondrial cytochrome c oxidase subunit I (COI) of N. hera and compared nucleotide variation with its congeners. The intra-specific genetic distance between the three COI sequences of N. hera was ranged from 0 to 0.4%. The inter-specific distances between N. hera and other Nereiphylla species ranged from 18.8 to 22.3%. In this study, we reported the first COI barcodes of N. hera with the morphologcial diagnosis and the photographs. These results would be helpful to understand taxonomy of Nereiphylla.

• Animal Systematics, Evolution and Diversity
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• 제38권4호
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• pp.149-156
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• 2022

The magelonid polychaete Magelona parochilis Zhou and Mortimer, 2013 is newly reported from South Korea. The Korean specimens correspond well to the original description of M. parochilis in having prostomium without horn, mucronate chaetae on chaetiger 9, superior dorsal lobes on chaetigers 1-8, ventral neuropodial lobes on chaetiger 9, smooth edged thoracic notopodial lamellae, and unidirectional tridentate hooded hooks on abdominal chaetigers. Partial sequences of cytochrome c oxidase I (COI) and 16S ribosomal DNA (16S rDNA) of the species were determined from Korean specimens. The detailed description and illustrations are provided with partial sequences of COI and 16S rDNA as molecular markers for species identification.

• Animal Systematics, Evolution and Diversity
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• 제39권3호
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• pp.118-122
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• 2023

The genus Hydroporus Clairville is the most diverse group belonging to the subfamily Hydroporinae in the family Dytiscidae. However, members of this group have not been recorded in the Korean peninsula. The genus Hydroporus Clairville is characterized by the following features: body glabrous to distinctly setose; elytra and ventral surface mainly piceous to black; epipleuron lacking a carina on humeral angle; metacoxal process conjointly with hind margin straight or slightly angulate medially. We collected the high mountain wetland and identified the species of Hydroporus uenoi Nakane based on male genitalia for the first time. A habitus photo, a redescription, mitochondrial DNA sequence, illustrations of the aedeagus of species, and habitat information are provided.

• 식물분류학회지
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• 제34권1호
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• pp.63-74
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• 2004

나문재속은 염습지에서 가장 흔히 자라는 식물인데, 한국산 나문재속은 형태적 변이가 심하여 종 식별이 어렵다. 본 연구는 RAPD분석을 통해 한국산 나문재속의 종간 분류학적 한계를 명확히 하고, 서, 남해안에서 칠면초 집단간의 유전적 변이를 알아보고자 수행되었다. 실험에 사용된 6개의 primer로부터 65개의 유용한 band를 얻었는데, 그 중 61개가 다형성이었다. RAPD 분석결과 Schanginia절에 속하는 나문재는 Heterosperma절에 속하는 나머지 종들과 조사된 모든 primer에서 뚜렷한 차이를 보였다. 또한 외부형태적으로 구별이 어려운 칠변초, 해홍나물, 기수초에서 종간에 차이가 있는 DNA band가 발견되었다. 칠면초 집단에 대한 조사에서 지역 집단간에 차이를 보이는 RAPD marker가 나타났으나, 지역 내 생육지에 따른 변이는 없었다.

• 식물분류학회지
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• 제48권4호
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• pp.289-300
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• 2018

Coreanomecon is a monotypic and endemic genus in Korea, distributed mainly in the southern regions. Coreanomecon is morphologically similar to Hylomecon by producing red latex, easily distinguished from Chelidonium, which produces yellow latex. Coreanomecon were merged into Hylomecon or Chelidonium depending on the authors. To understand the phylogenetic relationship of Coreanomecon, DNA sequences of chloroplast rbcL and matK and nuclear Internal Transcribed Spacer (ITS) regions were determined from the species of Papaveroideae (Papaveraceae) in Korea and analyzed with the Maximum Parsimony and Bayesian methods. Phylogenetic analyses of Papaveroideae suggest that Coreanomecon is sister to the clade of Chelidonium and Stylophorum in the ITS data and that it is sister to Hylomecon in the chloroplast (cpDNA) data. A constraining analysis using the Shimodaira-Hasegawa test (S-H test) suggested that the ITS data do not reject the sister relationship of Coreanomecon and Hylomecon. The S-H test also suggested that the cpDNA data is compatible with the placement of Coreanomecon as a sister to the clade of Chelidonium and Stylophorum. Although the conflicting phylogenetic results may stem from insufficient phylogenetic signals, they may also be associated with hybridization between Hylomecon and an ancestor of Stylophorum and Chelidonium. The results of this study suggest that Coreanomecon is a distinct lineage as an endemic genus, supporting the morphological data.

• 한국자원식물학회지
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• 제19권6호
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• pp.716-720
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• 2006

The genetic diversity among the genus Viola was evaluated using the random amplified polymorphic DNA (RAPD) method. A total of 142 distinct amplification fragments by 18 random primers were scored to perform the cluster analysis with UPGMA. Viola species from the subsection Patellares were clustered into group I to IV. The groups from I to IV were consistent with its morphological taxonomy, series Pinnatae, Chinensis, Variegatae, and Patellares in the subsection Patellares, respectively. Even though V. albida and V. albida var. takahasii were classified in Chinensis, they were assigned into group I. The cluster analysis separated other subsections from Patellares in the section Nomimium. Interestingly, V. verecunda and V. grypoceras in subsections Biobatae and Trigonocarpae, respectively, were clustered into group C with a high similarity coefficient. Therefore, RAPD analysis can be used for providing an alternative classification system to identify genotypes and morphological characters of Viola species.

• Animal Systematics, Evolution and Diversity
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• 제37권3호
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• pp.219-224
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• 2021

A hesionid species, Leocratides kimuraorum Jimi, Tanaka and Kajihara, 2017 is newly reported from the sublittoral zones (100 m depth) of the Korean coasts. This species is characterized by lateral antennae as long as the palps, peristomial membrane without papillose, peristomial dorsolateral tubercles with two round marginal lobes, and pharyngeal with terminal papillae. The intra-specific genetic distance among the cytochrome c oxidase subunit I(COI) sequences of L. kimuraorum specimens from Japan (type locality) and Korea (this study) was in the range of 0.002-0.005. The inter-specific genetic distance between L. kimuraorum and other hesionid species were 0.166-0.307. The present study is the first record of Leocratides species in Korean fauna. This paper also provides a morphological description and photographs of L. kimuraorum, with partial sequences of the mitochondrial COI based on Korean specimens.

• 식물분류학회지
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• 제46권3호
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• pp.273-282
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• 2016

The establishment of a DNA barcode database at the regional scale and assessments of the utility of DNA barcodes are crucial for conservation biology and for the sustainable utilization of biological resources. Schisandraceae is a small family consisting of ca. 45 species. It contains many economically important species, such as Schisandra chinensis, which is widely used as a source in tonic beverages and in oriental medicine. In Korea, three species, S. chinensis, S. repanda, and Kadsura japonica, are distributed. We evaluated the level of variation of the DNA sequences of rbcL, matK, and the ITS regions from 13 accessions representing the distributional range of the three species. The three DNA barcode regions were easily amplified and sequenced. The minimum values of the interspecific genetic distances among S. chinensis, S. repanda, and K. japonica either separately or in combination are 4- to 23-fold higher than the maximum value of the intraspecific distance, showing that there is a clear DNA barcoding gap in the regions for Korean Schisandraceae. Phylogenetic analyses of the three DNA barcode regions, separately and simultaneously, indicate that all of the DNA barcode regions are useful for identifying a species of Schisandraceae in Korea. The distinctiveness of the three species of Schisandraceae was also supported at the species level when Chinese and Japanese populations were added. The results of this study indicate that three concatenated regions constitute the best option for DNA barcoding in Schisandraceae in Korea.

• 식물분류학회지
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• 제45권2호
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• pp.158-168
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• 2015

한국산 물통이(Pilea)속 식물의 분자계통학적 연구를 통해서 총 1속 5분류군으로 정리하였다. 물통이속은 모두 1년생 초본으로, 그늘지고 습기가 있는 지역에서 서식하며, 여름에 꽃이 피고, 가을에 열매를 맺는다. nrDNA의 ITS regions과 cpDNA의 psbA-trnH regions의 DNA 염기서열의 분석 결과, 산물통이, 물통이는 분계조를 각각 형성하였다. 하지만 제주 산방산의 제주큰물통이는 내륙지역의 지리산에서 자생하는 제주큰물통이와 같은 분계조를 형성하지 못하고 큰물통이, 모시물통이와 섞여 분계조를 형성하였다. ITS1, 4 regions에서만 DNA 염기서열이 분석된 지리산의 제주큰물통이 역시 완전히 다른 분계조를 형성하였다. 단순히 지리적인 차이로 인해 형성되었다고 보기에 무리가 있을 것으로 생각되어지며, 후에 좀 더 많은 연구가 이루어져야 할 것으로 생각되어진다.