• Title/Summary/Keyword: DNA microarray analysis

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Isolation and Characterization of Parvalbumin Beta Gene from Channel Catfish (Ictalurus punctatus)

  • Kim, Soon-Hag
    • Journal of Aquaculture
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    • v.16 no.2
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    • pp.124-127
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    • 2003

  • Our previous studies of both microarray analysis in channel catfish muscle gene expression of 2 different ages and channel catfish muscle expressed sequence tag profiles demonstrated parvalbumin beta is one of the highly expressed muscle transcriptome. We have cloned and sequenced complementary DNA encoding the channel catfish parvalbumin which encode 109 amino acids. The deduced amino acid sequences of the catfish parvalbumin are highly conserved with those cloned from other teleosts. The availability of the catfish parvalbumin provides the opportunity of studying fish epitopes.

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Expression of osteopontin and this role in hepatic stellate cell motility and wound healing migration

  • Lee, Sung-Hee;zhe, Zhao-Yu;Park, Eun-jeon;Min, Song-Hye;Hwan, Sohn-Dong
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.142.2-142.2
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    • 2003

  • The activation of the hepatic stellate cell (HSC) is a key step in liver fibrogenesis. We investigated the changes of global gene expression during activation in hepatic stellate cells using a rat cDNA microarray with 5, 000 sequence-verified clones. We identified osteopontin (OPN), a secreted matrix protein, as one of the upregulated factors. Northern analysis showed OPN mRNA was increasingly expressed during progressive activation of cultured rat HSCs and in models of experimental liver fibrosis. (omitted)

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