• 대한한의학회지
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• 제32권5호
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• pp.41-49
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• 2011

Objectives: To optimize the preservation method of herbal decoction, we investigated the content of principle components of Pyungwi-san, liquiritin, glycyrrhizin, and hesperidin according to preservation temperature and period. We also investigated the changing patterns of pH and microbial population in Pyungwi-san decoction as a model case. Methods: With samples preserved at different temperatures, the content of liquiritin, glycyrrhizin, and hesperidin was determined using HPLC and microbial population was determined as viable counting method up to 8 times every month. Identification of isolated bacteria was performed by 16S rDNA analysis. Results: The content of liquiritin and glycyrrhizin did not change according to the preservation temperature and period, but that of hesperidin was severely decreased at room temperature. The isolate from the decoction was identified as Bacillus licheniformis by 16S rDNA sequence analysis. Microbial population appeared after 3 months' preservation and reached maximum value at 4 months; at all tested temperatures, the pH showed the lowest value (4.4-4.5) simultaneously. Conclusion: From the results, it seems to be that the microbial growth affects the pH of preserved decoction but not the change of liquiritin and glycyrrhizin content.

• 생명과학회지
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• 제8권1호
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• pp.32-39
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• 1998

진딧물이 하나의 ribosomal RNA 유전자(rDNA)단위는 총 길이가 13,061bp이며 총 G/C비율은 59%이다. 그것을 구성하고 있는 각 영역의 길이와 G/C비율은 다음과 같다. 5’ETS는 G/C비율이 69%이고 843bp이다 . 18S rRNA 는 2,469bp이며 G/C비율은 59%이다. ITS I길이는 229bp이며 70%의 G/C비율이다. 5.8S rRNA는 160bp이며 63%의 G/C비율이다. ITS II는 325bp이며 70%dml G/C비율이다. 28S rRNA는 4, 147bp이고 60%의 G/C비율이다. IGS는 4,888bp로 55%의 G/C비율이다.

• Journal of Ginseng Research
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• 제17권1호
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• pp.39-44
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• 1993

In Korean ginseng, Panax ginseng C.A Meyer, it was difficult to isolate chloroplast DNA with classical methods, because of the high polysaccharide content of ginseng chloroplast The simple and efficient method of chloroplast DNA isolation from ginseng leaves has been developed by motificalion of recently advanced methods. Also, it can be successfully applied to ctDNA isolation of Chinese cabbage, radish, petunia tobacco as well as ginseng. Isolated chloroplast DNA from ginseng was digested with various restriction endonucleases. It was estimated that the molecular weight of Korean ginseng chloroplast DNA was about 142 kb. There was no difference in restriction endonuclease digestion patterns between two variants of Korean ginseng, which are Jakyung-Jong (violet-stem variant) and Hwang- sook-Jong (yellow-berry variant).

• Preventive Nutrition and Food Science
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• 제17권2호
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• pp.116-121
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• 2012

Water extract from Pinus densiflora (WPD) was investigated for its antioxidant activity and its ability to provide protection from DNA damage. A series of antioxidant assays, including a 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging assay, a reducing power assay, a metal-chelating assay, a superoxide radical scavenging assay, and a nitrite scavenging ability, as well as a DNA damage protection assay were performed. Total phenolic content was found to be 211.32 mg Tan/g WPD. The extract scavenged 50% DPPH free radical at a concentration of 21.35 ${\mu}g/mL$. At that same concentration, the reducing power ability of WPD was higher than that of ${\alpha}$-tocopherol. The extract chelated 68.9% ferrous ion at the concentration of 4 mg/mL. WPD showed better nitrite scavenging effect at the lower pH. Meanwhile, WPD exhibited a strong capability for DNA damage protection at 1 mg/mL concentration. Taken together, these data suggest water extract from Pinus densiflora could be used as a suitable natural antioxidant.

• 한국약용작물학회지
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• 제24권6호
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• pp.464-470
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• 2016

Background: Valeriana fauriei (Valerianaceae) has been used to as a traditional medicine to treat a variety of symptoms, including headache, insomnia, hypertension, and menstrual irregularity. However, the present study investigates the species' antioxidant activity and its inhibition of oxidative DNA damage, which have yet to be studied. Methods and Results: The antioxidant activity was assessed using radical scavenging assays with 1,1-diphenyl-2-picryl hydrazyl (DPPH) and, 2, 2'-azino-bis (3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt (ABTS) and a reducing power assay. The total phenol content was also analyzed, and phenolic compounds were detected using HPLC/UV, whereas the inhibitory effect of Valeriana fauriei on oxidative DNA damage was measured using ${\phi}-174$ RF I plasmid DNA cleavage assay. The DPPH and ABTS radical scavenging activity were $75.17{\pm}3.55%$ and $95.83{\pm}0.63%$, repectively, and the reducing power was $93.14{\pm}1.74$ at $200{\mu}g/m{\ell}$. The total phenol content was $10.24{\pm}0.04mg/g$, whereas chlorogenic acid, catechin, caffeic acid and epicatechin were identified using HPLC/UV, and the ${\phi}-174$ RF I plasmid DNA cleavage assay indicated that V. fauriei provided protection against oxidative damage. Conclusions: The results of the present study suggest that V. fauriei has powerful antioxidant activity that can provide protective effects against the oxidative DNA damage caused by free radicals. The species, therefore, provides a valuable resource for the development of natural pharmaceutical to treat aging, cancer, and degenerative diseases.

• Journal of Photoscience
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• 제9권2호
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• pp.150-153
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• 2002

Solar UV light is a well-known carcinogen. UVA radiation is probably carcinogenic to humans. In addition, recent investigations point to the importance of UVA irradiation in the photoaging. We investigated the mechanism of sequence- specific DNA damage using $\^$32/P-Iabeled DNA fragments in relation to carcinogenesis and aging. Furthermore, we investigated whether UVA accelerates the telomere shortening in human WI-38 fibroblasts. The exposure of double- stranded DNA fragments to 365 nm light in the presence of endogenous sensitizers produced sequence-specific cleavage at the 5' site of 5'-GG-3' and 5'-GGG-3' sequences. In addition, HPLC analysis revealed that sensitizers plus 365 nm light increased the 8-oxodG content of double-stranded DNA. We discuss the mechanisms of guanine-specific DNA damagecaused by excited photosensitizers in relation to carcinogenesis and aging.

• Bulletin of the Korean Chemical Society
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• 제29권6호
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• pp.1233-1242
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• 2008

The hydration effect on the intrinsic magnetism of natural salmon double-strand DNA was explored using electron magnetic resonance (EMR) spectroscopy and superconducting quantum interference device (SQUID) magnetic measurements. We learned from this study that the magnetic properties of DNA are roughly classified into two distinct groups depending on their water content: One group is of higher water content in the range of 2.6-24 water molecules per nucleotide (wpn), where all the EMR parameters and SQUID susceptibilities are dominated by spin species experiencing quasi one-dimensional diffusive motion and are independent of the water content. The other group is of lower water content in the range of 1.4-0.5 wpn. In this group, the magnetic properties are most probably dominated by cyclotron motion of spin species along the helical π -way, which is possible when the momentum scattering time (${\tau}_k$) is long enough not only to satisfy the cyclotron resonance condition (${\omega}_c{\tau}_k$ > 1) but also to induce a constructive interference between the neighboring double helices. The same effect is reflected in the S-shaped magnetization-magnetic field strength (M-H) curves superimposed with the linear background obtained by SQUID measurements, which leads to larger susceptibilities at 1000 G when compared with the values at 10,000 G. In particular, we propose that the spin-orbital coupling and Faraday's mutual inductive effect can be utilized to interpret the dimensional crossover of spin motions from quasi 1D in the hydrate state to 3D in the dry state of dsDNA.

• 한국응용곤충학회지
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• 제10권2호
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• pp.103-107
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• 1971

구기자응애 (Eriophyes kuko Kishida)의 침입 기생에 의하여 구기자나무(Lycium chinense Mill)의 잎에 형성되는 혹(Mite gall)의 성장에 따르는 표피세포와 핵의 크기를 측정하는 동시에 핵산(DNA, RNA)의 함량(함량으로 표시되느 농도분포)을 현미분광측정기(Microspectrophotometer)로 측정한 그 결과를 요약하면 다음과 같다. 1. 혹이 성장함에 따라 표피세포와 핵이 커지며 건전한 것보다 크게 된다. 2. DNA의 함량(함량으로 표시되는 농도분포)은 혹이 형성되는 초기에는 별로 변동이 없는 것 같으나 성장한 혹에 있어서는 건전한 것보다 오히려 증가하는 경향이 보였다. 3. RNA의 함량(함량으로 표시되는 농도분포)은 혹이 형성되는 초기부터 중기까지는 일시 적으로 증가하는 것 같으나 혹이 성장하게되면 건전한 것보다 오히려 감소되는 경향이 보였다.

• 한국산림과학회지
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• 제102권2호
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• pp.198-203
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• 2013

바이오매스 생산과 분자육종 연구를 위한 소재로 육성된 3배체 현사시나무(Populus alba ${\times}$ P. glandulosa $F_1$)의 핵 DNA 함량과 조직학적 특성을 조사하였다. 유세포 분석 결과 3계통의 3배체 현사시나무(Line-1, Line-17, Line-18)의 핵 DNA 함량이 2배체 현사시나무에 비하여 1.6배 이상 큰 것으로 나타났다. 조직학적 분석 결과 3배체 현사시나무는 계통별로 조금 다른 차이를 나타내었지만 'ine-18'은 줄기 단면적이 2배체 현사시나무에 비해 1.6배 이상 넓고, 수와 피층/사부의 면적이 2.0배와 1.6배 이상 각각 증가한 것으로 나타났다. 또한 'ine-18'은 잎의 공변세포의 길이와 면적이 2배체에 비하여 1.2배 이상 증가한 것으로 나타났다. 본 연구결과는 3배체 포플러의 계통 육성을 통한 바이오매스 생산 및 산업적 이용 가능성을 탐색하기 위한 기초자료로 이용될 것으로 기대된다.

• Asian-Australasian Journal of Animal Sciences
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• 제1권3호
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• pp.139-142
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• 1988

Effects of anthranilic acid on normal mammary gland growth were examined in SHN/Mei female virgin mice. Anthranilic acid was given to the experimental groups as drinking water at the concentrations of 0.01, 0.02 or 0.04% for 21 days beginning 2-3 months of age. The control group received tap water only. RNA content and RNA/DNA ratio in mammary glands were significantly higher in mice given 0.04% anthranilic acid than in the control, while not mammary DNA content. The results indicate that chronic ingestion of anthranilic acid can induce an enhancement of proliferation and differentiation of mammary cells.