• 대한화학회지
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• 제33권2호
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• pp.192-202
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• 1989

네자리 Schiff base cobalt(II) 착물로서 Co(SED) 및 Co(ο-BSDT)들을 합성하여 이들 착물들의 DMSO와 Pyridine 용액에서 산소를 가하여 산소첨가 생성착물로서 $[Co(o-BSDT)(DMSO)]_2O_2,\;[Co(SED)(Py)]_2O_2$ 및 $[Co(o-BSDT)(Py)]_2O_2$들을 합성하고 이들의 원소분석과 cobalt 정량, IR Spectra, TGA 및 자화율을 측정하여 DMSO 용매에서는 산소 : cobalt(II) 착물의 결합비가 1 : 2이고 pyridine 용매에서의 첫단계는 1 : 1이지만 저온과 오랜 반응시간에는 1 : 2로 주어진다. 또한 네자리 Schiff base cobalt(II)는 DMSO 및 pyridine과 산소가 6배위 결합으로 주어짐을 알았으며 Co(SED) 및 Co(ο-BSDT) 착물들의 0.1M TEAP-DMSO와 0.1M TEAP-pyridine 용액에서 순환전압-전류법에 의한 산화-환원과정은 산소가 결합되지 않은 착물에서 Co(II)/Co(III)와 Co(II)/Co(I) 과정은 가역적 또는 준가역적으로 일어나지만 산소첨가 생성착물은 비가역적으로 일어나고 산소결합의 환원전위는 $E_{pc}=-0.85{\sim}-1.19V$에서 일어나고 이에 couple인 산화전위는 $E_{pa}=-0.74{\sim}-0.89V$에서 준가역적으로 일어남을 알았다.

• Clinical and Experimental Reproductive Medicine
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• 제31권1호
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• pp.59-65
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• 2004

Objectives: The aim of this study was to assess toxicities of cryoprotectants. Methods: Toxicities of two cryoprotectants, dimethyl sulfoxide (DMSO) and 1, 2-propanediol (PROH), were investigated using a murine embryo model. Female F-1 mice were stimulated with gonadotropin, induced ovulation with hCG and mated. Two cell embryos were collected and cultured after exposure to either DMSO or PROH. Embryo development was evaluated up to the blastocyst stage. Blastocysts were stained with bis-benzimide to evaluate the cell count and with terminal deoxynucleotidyl transferase mediated dUTP nick labeling (TUNEL) to assess apoptosis. Results: The total cell count of blastocysts that were treated with DMSO at the 2-cell stage was significantly lower than that were treated with PROH ($75.9{\pm}27.0$) or the control ($99.0{\pm}18.3$) (p<0.001). On comparison of two cryoprotectant treated groups, the DMSO treated group showed a decreased cell count compared with the PROH treated group (p<0.05). Both DMSO ($14.2{\pm}1.5$) and PROH ($11.2{\pm}1.4$) treated groups showed higher apoptosis rates of cells in the blastocyst compared with the control ($6.2{\pm}0.9$, p<0.0001). In addition, the DMSO treated group showed more apoptotic cells than the PROH treated group (p<0.001). Conclusions: The potential toxicity of cryoprotectants was uncovered by prolonged exposure of murine embryos to either DMSO or PROH at room temperature. When comparing two cryoprotective agents, PROH appeared to be less toxic than DMSO at least in a murine embryo model.

• 한국발생생물학회지:발생과생식
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• 제16권2호
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• pp.77-85
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• 2012

담수에서 사육한 감성돔 Acanthopagrus schlegeli(담수감성돔) 냉동/해동 정자의 생존율이 가장 높았던 결빙억제제(cryprotective agent, CPA)는 dimethyl sulfoxide(DMSO)였으며, 다음으로 glycerol, ethylene glycol(EG) 그리고 methanol 순이었다. 해동 후 정자운동성은 glycerol, DMSO, EG, methanol 순으로 높았으며, 이상의 결과들은 해수에서 사육한 감성돔(해수감성돔)에서도 나타났다. 담수 및 해수감성돔 정자의 냉동보존시 적정 CPA는 DMSO와 glycerol이었으며, 생존율과 정자운동성이 가장 높았던 적정 농도는 모두 10%였다. 특히 10% DMSO로 냉동보존한 담수 및 해수감성돔 해동정자의 생존율과 정자운동성은 10% glycerol로 냉동보존한 해동정자보다 높게 나타났다. 냉동보존기간에 따른 담수 및 해수감성돔 해동정자의 생존율과 정자운동성을 측정한 결과, 대부분 보존기간이 길어질수록 감소하였으며, CPA의 농도가 높을수록 생존율과 정자운동성은 급격히 감소하였다. 그러나 5% DMSO로 냉동보존한 해동정자의 생존율 및 정자운동성은 차이는 보이지 않았다.

• 한국식품과학회지
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• 제50권1호
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• pp.1-7
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• 2018

본 연구에서는 식품성분이나 천연물의 세포활성 측정에 널리 사용되는 MTT 분석 상의 최종산물인 MTT 포마잔 색소에 대한 여러 용매 상에서의 안정성과 다양한 빛과 ZnPP가 미치는 영향을 조사하였다. MTT 포마잔의 발색도는 0.01 N 이상의 NaOH 농도에서 가장 높게 나타났으며, 증류수, DMSO, 에탄올, 메탄올 등의 용매에 비해 2배 이상의 발색강도를 보였다. 증류수나 0.01 N 이하의 NaOH 농도에서 MTT 포마잔은 불안정하여 빛의 조사와 관계없이 신속하게 분해되었으며 0.1 N NaOH, DMSO에서는 안정하였다. DMSO나 0.1 N NaOH 상에서 MTT 포마잔은 형광등과 UVA (365 nm)의 조사에 의해 2시간 내에 발색도의 현저한 변화가 관찰되지 않았으나, UVB (254 nm)에 노출되었을 경우 용매와 관계없이 신속하게 탈색되었다. MTT 포마잔은 에탄올, 메탄올, DMSO와 NaOH 용매상에서 ZnPP에 의해 시간과 농도에 의존적으로 분해되었으며, 0.1 N NaOH 상에서 가장 민감하고 신속한 탈색 양상을 나타내었다. DMSO와 0.1 N NaOH 상에서 MTT 포마잔은 $0.1{\mu}M$의 ZnPP에 의해서도 유의적인 탈색현상을 나타내었다. 하지만 ZnPP에 의한 MTT 포마잔의 탈색현상은 암소에서는 일어나지 않았으며, 빛과 관계없이 명소와 암소에서 모두 수용성 XTT 포마잔에는 미미한 효과를 나타내었다.

• 한국자원식물학회지
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• 제25권6호
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• pp.693-699
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• 2012

This study was conducted to investigate the effects of Woohwangcheungsimweun (ox bezoar), deer antlers, and wild ginseng on induction of cardiomyocyte differentiation using the established mouse embryonic stem (ES) cells. The expression of atrial natriuretic peptide (ANP) was highest in Woohwangcheungsimweun treatment group. The expression of rabbit anti-GATA-4(GATA-4) and troponin (TnI) were highest in wild ginseng and Woohwangcheungsimweun treatment groups, respectively. Fluorescence activated cell sorting (FACS) analysis showed that the expression of ANP was highest in Dimethyl sulfoxide(DMSO) and Woohwangcheungsimweun treatment groups. The expression of GATA-4 was relatively high in wild ginseng treatment group. The expression of TnI was highest in Woohwangcheungsimweun treatment group. In the gene expression analysis, DMSO greatly inhibited GATA-4 expression to 25% of control. Woohwangcheungsimweun treatment caused to increase cTnI and cardiac ANP expression significantly. Wild ginseng extract upregulated GATA-4 gene expression. In conclusion, DMSO widely used as cardiomyocyte differentiation inducer did not show significant effects on the expression of ANP, GATA-4 and TnI in this study. Woohwangcheungsimweun showed upregulation of ANP and TnI expression. Wild ginseng extract showed greater effects than DMSO on GATA-4 expression. These results might suggest that the combination of Woohwangcheungsimweun and wild ginseng extract treatment can be expected to increase expressions of all three genes.

• Bulletin of the Korean Chemical Society
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• 제26권12호
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• pp.2001-2006
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• 2005

Glycoproteins and glycolipids play key roles in intracellular reactions between cells and their environments at the membrane surface. For better understanding of the nature of these events, it is necessary to know threedimensional structures of those carbohydrates, involved in them. Since carbohydrates contain many hydroxyl groups which can serve both as hydrogen bond donors and acceptors, hydrogen bond is an important factor stabilizing the structure of carbohydrate. DMSO is an aprotic solvent frequently used for the study of carbohydrates because it gives detailed insight into the intramolecular hydrogen bond network. In this study, conformational properties and the hydrogen bonds in $\beta$-lactose in DMSO are investigated by NMR spectroscopy and molecular dynamics simulations. NOEs, temperature coefficients, deuterium isotope effect, and molecular dynamics simulations proved that there is a strong intramolecular hydrogen bond between O3 and HO2' in $\beta$-lactose and also OH3 in $\beta$-lactose may form an intermolecular hydrogen bond with DMSO.

• 한국가축번식학회지
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• 제14권1호
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• pp.9-16
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• 1990

This study was carried out in order to investigate effects of cryoprotectant concentration and equilibration time on survival of ultrarapidly frozen 2-cell mouse embryos. Mouse 2-cell embryos, following dehydration by exposure to DMSO and sucrose, were directly immersed into liquid nitrogen and thawed in 37$^{\circ}C$ water. Viability was defined by development rate to the blastocyst stage after in vitro culture for 72 hours. The results are summarized as follows ; 1. When 0.25M of sucrose was added into the freezing medium at various concentrations of DMSO and dilution medium, higher development rate of embryo was obtained in 3.0M DMSO concentrations (82.6%). However, when sucrose concentraitons of 0.25 and 0.5 M were added to the freezing medium with 3.0 M DMSO and dilution medium, development rate of embryos were 81.7% and 24.1%, respectively. 2. In the equilibration time at room temperature, higher development rate was attained after short period of time (2.5min) in 3.0 M DMSO＋0.25 M sucrose (85.9%). 3. The development rate of embryos at in vitro 2-cell, in vitro 2-cell, solution control and untreated control was 84.6%, 90.9%, 89.9%,, and 89.7%, respectively.

• Archives of Plastic Surgery
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• 제33권1호
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• pp.31-38
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• 2006

The effect of melatonin on morphological changes after ischemia-reperfusion injury was investigated in rat skeletal muscle. Dimethyl-sulfoxide(DMSO) was also tested for comparison. Muscle injury was evaluated in 4 groups as a single laparotomy group(control), ischemia-reperfusion group, DMSO group, melatonin group. Left hind limb ischemia was induced for 4 hours by vascular clamping of the common femoral artery and followed by 24 hours of reperfusion. The midportion of gastrocnemius muscle was taken for histological evaluation. In light microscopic study, ischemia-reperfusion group showed severe neutrophil infiltration, interstitial edema, and partial loss or degeneration of muscle fibers. The muscle tissue of melatonin group showed relatively normal architecture with mild inflammatory cell infiltration. In electron microscopic study, dilated cisternae of sarcoplasmic reticulum, dilated mitochondria with electron loose matrix and dilated cristae, disordered or loss of myofilament, indistinct A-band and I-band, intracytoplasmic vacuoles, and markedly decreased glycogen granules were observed in ischemia-reperfusion group. But relatively well maintained A-band, I-band, Z-line, M-line, and mildly dilated mitochondria with well preserved cristae were observed in melatonin group. The DMSO group showed intermediately attenuated ultrastructural changes. The results show that melatonin improves morphologically ischemia-reperfusion injury more effectively than DMSO. In conclusion, melatonin seems to be a promising agent that can salvage the skeletal muscle from severe ischemia-reperfusion injury.

• 펄프종이기술
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• 제45권2호
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• pp.21-26
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• 2013

Conversion of fructose to 5-hydroxymethylfurfural (5-HMF) was investigated in dimethylsulfoxide (DMSO) solvent with increasing reaction temperatures and impact of residual water from dehydration reaction byproduct. To convert fructose to 5-HMF, increasing reaction temperature led more conversion to 5-HMF than lower temperature at the range of $120-150^{\circ}C$ in DMSO solvent. DMSO engaged in the acid-catalyzed dehydration and rearrangement reaction as acid and solvent. Increasing temperature led to more furanose structure than pyranose at the range of $30-80^{\circ}C$. Formed 5-HMF could be degraded to levulinic and formic acid at the presence of acid and water. Removal of water in reaction medium could prevent 5-HMF degradation.

• Archives of Plastic Surgery
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• 제32권6호
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• pp.683-688
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• 2005

The ischemia-reperfusion injury of the skeletal muscles is caused by generation of reactive oxygen during ischemia and reperfusion. Melatonin or N-Acetyl-5-methoxy- tryptamine is suggested to have antioxidant effects in several tissues. In present study, we examined the protective effect of melatonin in a rat hind limb ischemia-reperfusion injury. Dimethyl-sulfoxide(DMSO) was also tested for comparison. Ischemia was induced for 4 hours by vascular clamping and followed by 1 hour or 24 hours of reperfusion. Muscle injury was evaluated in 4 groups such as single laparotomy group(control), ischemia-reperfusion group, DMSO group, melatonin group. Eedema ratio and malondialdehyde(MDA) of muscle tissue and serum level of creatine kinase(CK), were measeured at the end of reperfusion. DMSO and melatonin group showed significant amelioration of edema and serum CK compared with ischemia-reperfusion group. The decreasing effect was more prominent in melatonin group. The muscle tissue MDA concentration is significantly lower in melatonin group than in ischemia-reperfusion group. The results show that melatonin prevents and improves ischemia-reperfusion injury more effectively in a rat hind limb than DMSO dose. Thus, clinically the melatonin may be used for a beneficial treatment of such injuries