• The Journal of Korean Medicine
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• v.32 no.4
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• pp.139-148
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• 2011

Objective: The purpose of this study was to investigate the effect of food intake on the Fourier components of radial pulse wave. Methods: Thirty-one healthy male subjects participated in this study. Radial pulse was measured using 3 dimensional pulse imaging system (DMP-3000) before, right after, 40 minutes after, 80 minutes after and 120 minutes after food intake. Fourier transform was performed and the frequency and amplitude of Fourier components were analyzed. Results: 1. The frequency and the amplitude of Fourier components of radial pulse wave increased significantly after food intake. 2. The frequency of Fourier components increased right after food intake and then gradually decreased as time passed, however the amplitude of Fourier components increased and maintained certain levels and patterns throughout the experimental period of 120 minutes. 3. The change ratios of the frequency and the amplitude of Fourier components after food intake varied with the pulse measuring locations. Conclusions: Food intake exerts an influence on radial pulse wave, resulting in increase of frequency and amplitude of Fourier components. The change ratios of the frequency and the amplitude of Fourier components after food intake varied with the pulse measuring locations.

• The Journal of the Society of Korean Medicine Diagnostics
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• v.16 no.3
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• pp.11-22
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• 2012

Objectives: The aim of this study was to elucidate the relationship between pulse types measured by pulse analyzer and several factors including smoking, drinking alcohol, exercises etc. Methods: The subjects were 71 persons who were tested by oriental health examination. A self-reported questionnaire was used to gather age, smoking, drinking alcohol, exercise state and gender. The pulse types were measured by DMP 1000. The index of stress and fatigue were measured by SA 3000. We analyzed the frequency of pulse types according to gender, ages, smoking, drinking alcohol etc by chi-square test with SPSS ver. 19.0. Results: In the main pulse types, there were significant differences according to age(p<0.05) and marginal differences according to drinking alcohol(p<0.1). In the secondary pulse types, there were marginal differences according to exercise, ages. But there was no significant differences according to gender, smoking, body mass index (BMI), accelerated photoplethysmography (APG) feature, stress and fatigue. Conclusions: This study suggests that the pulse types are affected by drinking alcohol, ages, exercise.

• Korean Journal of Acupuncture
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• v.33 no.3
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• pp.121-130
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• 2016

Objectives : Recently, people who have normal brachial blood pressure(BP) are being threatened by high-risk disease such as stroke. The aim of this study is to suggest that new method to assess systemic circulation. It can be performed by analyzing optimal blood pulse wave on 3 sites belonging to subjects that have normal BP. Methods : We respectively extracted main peaks(h1) of optimal blood pulse wave on left/right temporal artery(LR1=h1), radial artery(LR2=h1) and dorsalis pedis artery(LR3=h1). We obtained h1 from 30 subjects who are discreetly chosen and have normal BP. Main peak(h1) can be extracted by using 3D pulse imaging analyser(DMP-1000+, DAEYOMEDI Co., Korea) that has 5-level pressure method. We analyzed the ratio of [LR1/LR2] and [LR3/LR2]. Results : In the case of male group, the results are [LR1/LR2=0.7100.177] and [LR3/LR2=0.9290.317]. In the case of female group, the results are [LR1/LR2=0.6680.121] and [LR3/LR2=0.7050.195]. Especially, it is statistically verified that the result of ratio [LR3/LR2] is much higher in male group than in female group(p<0.05). Conclusions : We suggested the standard ratio of [LR1/LR2] and [LR3/LR2] for normal subjects, respectively. It can be adopted as a new method to evaluate the systemic circulation.

• International Journal of Stem Cells
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• v.15 no.4
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• pp.384-394
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• 2022

Background and Objectives: Dental pulp stem cells (DPSCs) play an important role in the repair of tooth injuries. Electrogenic sodium bicarbonate cotransporter 1 (NBCe1) is a Na⁺-coupled HCO₃^- transporter encoded by the solute carrier 4A4 (SLC4A4) gene and plays a crucial role in maintaining the pH of DPSCs. Our previous research confirmed that NBCe1 is highly expressed in odontoblasts during the development of the tooth germ. Therefore, in this study, we aimed to investigate the effect of NBCe1 on odontogenic differentiation of DPSCs and further clarify the underlying mechanisms. Methods and Results: DPSCs were isolated and identified, and the selective NBCe1 inhibitor S0859 was used to treat DPSCs. We used a cell counting Kit-8 assay to detect cell proliferative ability, and intracellular pH was assessed using confocal microscopy. Odontogenic differentiation of DPSCs was analyzed using real-time PCR and Alizarin Red S staining, and the NF-κB pathway was assessed using western blotting. Our results indicated that 10 µM S0859 was the optimal concentration for DPSC induction. Intracellular pH was decreased upon treatment with S0859. The mRNA expressions of DSPP, DMP1, RUNX2, OCN, and OPN were upregulated in the NBCe1 inhibited group compared to the controls. Moreover, NBCe1 inhibition significantly activated the NF-κB pathway, and a NF-κB inhibitor reduced the effect of NBCe1 on DPSC differentiation. Conclusions: NBCe1 inhibition significantly promotes odontogenic differentiation of DPSCs, and this process may be regulated by activating the NF-κB signaling pathway.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.6-6
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• 2001

Oocyte freezing has become a prevalent source for related reproductive technologies. This study was carried out to evaluate viability of post-thawed bovine oocyte injected DTT-treated sperm following by two different activation stimuli (Group 1, 5 M ionomycin, 5 min ＋ CR1aa, 3 h . 1.9 mM dimetylaminopurine (DMP), 3 h; group 2, ionomycin ＋ 10 $\mu\textrm{g}$/$m\ell$ cycloheximide(CHX), 5h). The techniques of ultra-rapid freezing used in this study were essentially similar to those of described by Vajta et al (Theriogenology 1999; 52:939-948), Denuded oocytes at 22 h of culture were exposed to cryoprotectant (3.2 M Ethylene glycol, 2.36 M DMSO, 0.6 M sucrose), and followed by freezing in electron microscopic grid. After thawing the oocytes were transferred back into the drop of maturation medium and cultured for additional 2 h before being subjected to ICSI. All eggs were then cultured in CRlaa medium, and transferred into M199+10% FCS on day 4. The culture was maintained until day 9. In Experiment 1, frozen-ICSI eggs were compared on development into blastocyst to those of unfrozen and IVF control. Those eggs were activated with the method of group 2. A higher proportion of unfrozen-ICSI and IVF eggs developed into cleavage and blastocysts than of frozen-ICSI eggs (65% and 13%; 71% and 23% vs. 39% and 8%; P<0.05). In Experiment 2, development and ploidy of embryos made from group 1 were compared to those from group 2. Between groups there did not differ on the rates of development, however, chromosomal abnormality in group 1 was significantly higher than in group 2 (49% vs. 30%; P<0.05). The present result suggests that frozen bovine oocytes can be used for ICSI.

• Journal of Acupuncture Research
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• v.32 no.2
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• pp.105-121
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• 2015

Objectives : The purpose of this study is to observe the effects of Sa-am acupuncture with tonifications of kidney and urinary bladder on radial pulse in healthy subjects. Methods : Sixty healthy subjects participated in this study, and were divided into a kidney tonifying acupuncture group(KI group), urinary bladder tonifying acupuncture group(BL group) and control group. Radial pulse was measured by 3 dimensional pulse imaging system(DMP-3000) before, immediately after, 30 minutes after, and 60 minutes after acupuncture at Cun, Guan, and Chi in each time. Results : 1. BL group exhibited significantly reduced T1 / T, T4 / T, T4 / (T-T4), T5 / T and increased(T-T4) / T compared to KI group. 2. BL group showed increase of radial augmentation index / heart rate(RAI / HR) in left Cun, H5, Ad / Ap in left Chi, H1, As / Ap in right Chi compared to KI group. BL group showed decrease of As / Ap, Aw / Ap in left Chi, H1, pulse depth, Ad / Ap in right Chi compared to KI group. 3. KI group showed increase of Aw / Ap, H1, H2, H4, pulse area in right Guan, pulse depth in right Chi compared to BL group. KI group showed decrease of RAI in left Cun, applied pressure, pulse depth in left Guan, Aw / Ap in right Chi compared to BL group. Conclusions : The effects of Sa-am acupuncture with tonifications of kidney and urinary bladder in healthy humans have been observed on various parameters. The parameters analysed in this study can be used to differentiate the effects of Sa-am acupuncture with tonifications of kidney and urinary bladder on radial pulse.

• Korean Journal of Acupuncture
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• v.28 no.3
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• pp.25-32
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• 2011

Objectives : The purpose of this study is to find parameters to compare the effects of Sa-Am acupuncture with stomach-tonification (ST) and Sa-Am acupuncture with spleen-tonification (SP) on radial pulse. Methods : Forty healthy subjects participated in this study. Radial pulse was measured using 3 dimensional pulse imaging system (DMP-3000) before, right after, 30 minutes after, and 60 minutes after acupuncture. Results : 1. The parameters analysed in this study were changed in the same direction with different degrees by ST and SP. 2. In males, SP manipulation showed significantly increased pulse period and (T-T4)/T, and significantly decreased T1/T, T4/T, T4/(T-T4), and F1~F3 frequencies compared to ST. 3. In females, ST manipulation showed significantly increased (T-T4)/T, and significantly decreased F1~F3 frequencies compared to SP. Conclusions : The parameters analysed in this study may be used to differentiate the effects between ST and SP on radial pulse. These parameters are more strongly affected by SP than ST in males whereas more strongly affected by ST than SP in females.

• Journal of Acupuncture Research
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• v.30 no.4
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• pp.1-14
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• 2013

Objectives : The purpose of this study is to investigate the effects of Sa-Am spleen-tonifying acupuncture on radial pulse in healthy human subjects. Methods : Forty healthy human subjects participated in this study, divided into acupuncture group and control group. Radial pulse was measured by 3 dimensional pulse imaging system(DMP-3000) before, right after, 30 minutes after and 60 minutes after acupuncture in the acupuncture group. The subjects in the control group didn't received acupuncture but took a rest and then the radial pulse was measured at the same time points as the acupuncture group. The parameters were analyzed by gender, measuring location, and measuring time point. However the time related parameters exceptionally were analyzed without distinction of measuring location. Results : 1. T, variance of period, T2/T, T4/T, (T-T4)/T, T4/(T-T4), and W significantly changed after acupuncture. 2. Pressure, amplitude of H1, amplitude of H2, Amplitude of H4 and pulse energy significantly changed after acupuncture. 3. Pulse area, systolic pulse area and diastolic pulse area significantly changed after acupuncture. 4. Elasticity, AIx and AIx/HR significantly changed after acupuncture. Conclusions : The effect of Sa-Am spleen-tonifying acupuncture in healthy human may be observed on time, amplitude, pulse area and augmentation index. Further studies on the effects of Sa-Am acupuncture using radial pulse are needed.

• Korean Journal of Acupuncture
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• v.27 no.1
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• pp.1-12
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• 2010

Objectives: The purpose of this study is to gain the objective indicators for the classification of hypertension by oriental medical pulse diagnosis, through finding out the parameters which can distinguish the pulse of hypertensive patient from that of normal subject, and characterizes the wiry pulse of hypertension. Methods: 30-59 yr, male, 946 healthy volunteers and 35 hypertensive patients were enrolled in this study. All the hypertensive patients were taking medicine to control the blood pressure and the blood pressure of the hypertension group was not statistically different from that of the normal healthy group. Data were acquired using 3 dimensional pulse imaging analyser(DMP-3000, DAEYOMEDI, KOREA) and analysed according to the age bands and the applied pressure levels. Results: 1. RAI/t, w/t and t2/t decreased with the levels of applied pressure and increased with age in the normal healthy group, not in the hypertension group. 2. RAI/t, w/t, t2/t were significantly higher in the hypertension group than the normal healthy group in the 30-age band, and elasticity coefficient was higher in the hypertension group than the normal healthy group in the 40-age band. 3. Researches on the patients without hypotensive agents are needed to figure out whether these parameters are the components of hypertensive wiry pulse. Conclusions: Analysing the radial pulse at 5 applied pressure levels using 3 dimensional pulse imaging analyser may be useful to differentiate the pulses of the hypertensive patients from those of the normal subjects, and characterize the hypertension.

• Journal of Korean Medicine for Obesity Research
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• v.23 no.1
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• pp.28-41
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• 2023

Objectives: This study was aimed to evaluate the hemodynamic feasibility using pulse parameters as a way to establish safe dose guidelines for herbal prescription containing Ephedra herb (Ephedra intermedia Schrenk & C.A.Mey) on weight loss, and to provide a foundation for developing evidence-based guidelines for clinical use. Methods: Forty-two volunteers were recruited to participate in a study examining the changes in pulse wave characteristics following the ingestion of Gambi-hwan, a herbal prescription containing ephedra, over a period of 4 weeks, and pulse wave measurements were taken before and after the administration. Pulse wave parameters were measured in this study using a 3-dimensional radial pulse tonometry device (DMP-Lifeplus). In addition, questionnaire, blood pressure, temperature, and body composition were also measured as secondary measures. Results: Fifteen minutes after administration of Gambi-hwan, the non-adverse event group (non-AE) exhibited a statistically significant increase in several power and pressure-related parameters, including h1, h5, systolic area, pulse area, and pulse width, while the AE group showed a trend of decreasing stroke volume and increasing Radial Augmentation Index (RAI), w, and w/t. After 4 weeks of administration, both groups exhibited significant changes in pulse rate, w/t, RAI, t3/t, stroke volume (SV), and stroke volume Index (SVI). Notably, there are significant differences between AE group and non-AE group in w/t, SV, and SVI. Conclusions: These findings suggest that pulse parameters may be a useful way to establish safe dosing guidelines for weight loss herbal prescription containing ephedra. Further research is needed to confirm these results and to develop evidence-based guidelines for clinical use.