• Title/Summary/Keyword: DMP

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Lactoferrin Constitutively Enhances Differentiation of Osteoblastic MC3T3-E1 Cells in Vitro

  • Yang, Hee-Young;Lee, Ha-Mi;Park, Byung-Ju;Lee, Tae-Hoon
    • International Journal of Oral Biology
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    • v.39 no.3
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    • pp.145-151
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    • 2014
  • During bone remodeling, there is requirement of differentiation of osteoblastic cells. Previously, we identified proteins differentially expressed in soft tissue during bone healing. Of these proteins, we focused the effect of LTF on differentiation of osteoblast. In order to analyze the osteogenic ability of LTF, we treated conditioned media collected from human LTF-stably transfected HEK293T cells into osteoblastic MC3T3-E1. The results showed that the activity and expression of alkaline phosphatase were increased in MC3T3-E1 cells treated with conditioned media containing LTF in dose- and time-dependent manner. At the same time, we observed the significant increase of the expression of osteoblastic genes, such as ALP, BSP, COL1A1, and OCN, and along with matrix mineralization genes, such as DMP1 and DMP2, in LTF conditioned media-treated groups. Moreover, the result of treating recombinant human LTF directly into osteoblastic MC3T3-E1 showed the same pattern of treating conditioned media containing LTF. Our study demonstrated that LTF constitutively enhances osteoblastic differentiation via induction of osteoblastic genes and activation of matrix mineralization in MC3T3-E1 cells.

Biological Monitoring of the Exposure Level of Organophosphorus and Pyrethroid Pesticides in Floriculture Workers and Florists (화훼작업 종사자의 유기인계 및 피레스로이드 살충제 노출에 대한 생물학적 모니터링)

  • Song, Jae Seok;Kwon, Ki Doo;Choi, Hong Soon;Yu, Ho Young
    • The Korean Journal of Pesticide Science
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    • v.18 no.1
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    • pp.41-47
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    • 2014
  • This study was performed to evaluate the exposure level of organophosphorus and pyrethroid pesticide to floriculture workers and florists. The urinary dialkylphosphates, metabolites of organophosphorus insecticides, including dimethylphosphate (DMP), diethylphosphate (DEP), dimethylthiophosphate (DMTP), diethylthiophosphate(DETP) and pyrethroids of metabolites, cis/trans DCCA, DBCA, and 3-PBA were analysed to evaluate the exposure of organophosphorus and pyrethroid pesticide to floriculture workers and florists. The concentration of DMP is highest in floriculture workers. but the concentration of DETP is highest in retail florist. The concentration of 3-PBA is highest in floriculture workers. The amount of organophosphorus and pyrethroid pesticide expusure is highest in flower workers, wholesale florist and retail florists are followed. The management for reducing pesticide exposure to floriculture workers and wholesale florist is required.

Evaluation of Exposure to Organophosphorus Pesticides According to Application Type and the Protective Equipment among Farmers in South Korea (일부 농업인에서의 농약살포방식 및 보호구 착용에 따른 유기인계 농약노출평가)

  • Lee, Jeeyoung;Roh, Sangchul
    • The Korean Journal of Pesticide Science
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    • v.20 no.2
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    • pp.172-180
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    • 2016
  • This study was conducted to evaluate the relations between the exposure level of organophosphates (OPs) pesticide and application factors among rural farmers. The urinary dialkylphosphates, metabolites of organophosphorus pesticides, including DMP, DEP, DMTP and DETP were analyzed by GC/MSD and GC/MS/MS. The DMP and DMTP were detected more in the use of a speed sprayer without cap than with a capped one. Also, the less farmers wore the personal protective equipment (PPE), the more these were detected. The amount of organophosphorus exposure was the highest in the use of a power sprayer. However, it was low when a farmer applied pesticides with a speed sprayer with cap and wore more PPE. In this study, the detection rate was analyzed by chi-square test, the exposure level of OPs was analyzed by a generalized linear model.

Short Aramid Fiber Reinforced Polyethylene and Epoxy Composity- I. Studies on the Curing Acceleration of Epoxy/Amine System by Differential Scanning Calorimeter (Aramid단섬유보강 고기능폴리에틸렌 및 에폭시 복합재료 제조- I.DSC에 의한 에폭시/아민계의 경화 촉진 연구)

  • Ha, Chan Sik;Lim, Seung Ha;Kim, Byung Kyu;Chin, Young Jo;Cho, Won Jei
    • Applied Chemistry for Engineering
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    • v.3 no.1
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    • pp.111-118
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    • 1992
  • The curing acceleration of epoxy/amine system was investigated by using differential scanning calorimeter(DSC), The epoxy, diglycidylether of bisphenol A (DGEBA) was cured with methylene dianiline (MDA) with or without accelerators. Two kinds of accelerators were tested for the study ; tris(dimethylaminomethyl)phenol (DMP - 30) and 3 - (3,4 - dichlorophenyl)-1, 1-dimethylurea (DIURON). Heats of reaction and glass transition temperatures ($T_g$) of the cured epoxy system were analyzed by DSC along with the estimation of activation energy by the dynamic DSC studies. It was found that DMP - 30 is more effective accelerator than DIURON which showed faster curing and lower activation energy.

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The Effect of Over-expression and Inactivation of Nuclear Factor I-C on the Dentin Matrix Gene Expression of MDPC-23 Odontoblasts (Nuclear Factor I-C 과발현과 발현억제가 MDPC-23 상아모세포주의 상아질 기질유전자 발현에 미치는 영향)

  • Bae, Hyun-Sook;Cho, Young-Sik
    • Journal of dental hygiene science
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    • v.9 no.4
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    • pp.427-433
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    • 2009
  • Nuclear factor I-C (NFI-C) null mice demonstrated aberrant odontoblast differentiation and abnormal dentin formation. In order to elucidate the mechanisms responsible for these changes, we evaluated the expression of dentin matrix gene after over-expression and inactivation of NFI-C in MDPC-23 cells by reverse transcription-polymerase chain reaction (RT-PCR) analysis. Collagen type I (Col I), osteocalcin (OC), and dentin sialophosphoprotein (DSPP) expression was decreased after inactivation of NFI-C. However, bone sialoprotein (BSP) expression was dramatically increased after inactivation of NFI-C. ALP and DMP4 expression was not changed after inactivation of NFI-C. The expression of alkaline phoshatase (ALP) and dentin matrix protein 4 (DMP4) was increased after over-expression of NFI-C, while Col I, OC, DSPP, and BSP expression was decreased. These findings suggest that odontoblasts after loss of NFI-C lost the phenotype of odontoblasts and acquired those of osteoblasts.

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Preparation and Characteristics of Poly(phenylene ether)s in Various Reaction Conditions (다양한 반응조건에 따른 폴리페닐렌에테르의 중합 특성)

  • Park, Jong-Hyun;Kim, Nam-Cheol;Kim, Yong-Tae;Nam, Sung-Woo;Kim, Young-Jun;Kim, Ji-Heung
    • Polymer(Korea)
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    • v.35 no.3
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    • pp.244-248
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    • 2011
  • Poly(2,6-dimethyl-1,4-phenylene ether) (PPE) was synthesized by oxidative polymerization using various Cu(I)-amine catalyst system. The effects of catalyst/monomer ratio, different amine ligand, and the content of 2,4,6-trimethylphenol (TMP) additive on the polymer yield and molecular weight were investigated by using gel permeation chromatography. The catalytic activity of various Cu-amine systems on the 2,S-dimethylphenol (DMP) polymerization was monitored and compared each other through oxygen-uptake experiment. In addition, the effect of catalyst removal using aqueous EDTA on the thermal stability of the prepared polymer was elucidated by thermogravimetric analysis.

Modification of Asphalt by in-situ Polymerization (내부중합에 의한 아스팔트바인더 개질 연구)

  • Lee, Sang-Yum;Mun, Sung-Ho;Jin, Jung-Hoon;Hong, Young-Keun
    • Elastomers and Composites
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    • v.46 no.3
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    • pp.257-261
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    • 2011
  • We introduce a novel method that could modify neat asphalt. A polymer-forming monomer, dimethylphenol( DMP) was added to the neat asphalt and polymerization was occurred autonomously, without adding any external catalyst for the polymerization, only with oxygen molecules in the air. The polymer produced in the asphalt was polyphenyleneoxide( PPO) and it enhanced the mechanical properties of the asphalt. Compared with the neat asphalt, the tenacity and toughness of the DMP-modified asphalt were two and half times and three times high, respectively.

Research on the Trend of Digital Contents Protection Techniques (디지털 컨텐츠의 보호기술에 관한 기술동향 분석)

  • Park, Jin;Na, Cheol-Hun;Jung, Hoe-Kyung
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • v.9 no.1
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    • pp.1094-1097
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    • 2005
  • It is issued that protecting and managing a copyright are very important to a digitization of multimedia contents. We analyze the trend of a multimedia service, IPMP(Intellectual Property Management and Protection), DMP(Digital Media Project), ENTHRONE(End-to-end Qos through Integrated Management of Content, Networks and Terminals), and DRM(Digital Rights Management). All of these techniques are for developing solution about protection and management of the copyright and media service. These have been at the head of the growth of the whole media service, but cause problems such as a illegal copy and distribution. To solve these problems, researchers are performing many projects.

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The Functional Requirements of Core Elements for Research Data Management and Service (연구 데이터 관리 및 서비스를 위한 핵심요소의 기능적 요건)

  • Kim, Juseop;Kim, Suntae;Choi, Sangki
    • Journal of the Korean Society for Library and Information Science
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    • v.53 no.3
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    • pp.317-344
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    • 2019
  • Increasing the value of data, paradigm shifts in research methods, and specific manifestations of open science indicate that research is no longer text-centric, but data-driven. In this study, we analyzed the services for DCC, ICPSR, ANDS and DataONE to derive key elements and functional requirements for research data management and services that are still insufficient in domestic research. Key factors derived include DMP writing support, data description, data storage, data sharing and access, data citations, and data management training. In addition, by presenting functional requirements to the derived key elements, this study can be applied to construct and operate RDM service in the future.

Analysis of Physicochemical and Sensory Properties of Dolsan Leaf Mustard Pickles (저장기간 동안 돌산 갓 피클의 이화학적 및 관능적 특성 분석)

  • Son, Hae-Reon;Oh, Sun-Kyung;Bae, Sang-Ok;Choi, Myeong-Rak
    • Journal of Life Science
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    • v.25 no.8
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    • pp.936-941
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    • 2015
  • The principal objective of this fundamental research was to analyze the physicochemical changes and sensory properties of Dolsan leaf mustard pickles (DMP) for the development of low-salt pickles in order to contribute to the diversification of food. The DMPs were stored at −2℃ for 90 days, with salinity maintained at 1.63% to 1.85%. The pH was decreased during storage, and the acidity was gradually increased for up to 60 days of storage, after which it was increased rapidly to 1.10. The L-values (lightness) from the stems were decreased during storage. The a-values (greenness) were increased, while the b-values (yellowness) were decreased. It was shown that the DMP had a progressive browning phenomenon and the number of lactic acid bacteria gradually decreased. The sensory evaluation showed that crunchy textures were decreased and touch textures were increased at 30 days. Therefore, Dolsan leaf mustard is suitable as a material for low-salt pickles during storage for 90 days. The results of the analysis of the physicochemical and sensory properties indicate that the optimal storage for DMP was maintained for up to 30 days.