• Journal of Acupuncture Research
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• v.29 no.2
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• pp.43-57
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• 2012

Objectives : The purpose of this study is to observe the effects of $Sa-Am$ lung sedating acupuncture (LS) on wrist pulse changes in healthy participants. Method : Forty healthy subjects participated in this study, and were divided into an acupuncture group and controlled group. Using a three-dimensional pulse imaging system (DMP-3000), wrist pulse was measured before, immediately after, 30 minutes after and 60 minutes after acupuncture in the acupuncture group, with the rest in controlled group. Sixteen parameters between the acupuncture group and the controlled group were analyzed at Cun, Guan and Chi in each time. Result : After LS acupuncture, wrist pulse sixteen parameters were changed significantly according to the time at each measuring region. 1. Heart rate significantly decreased in immediately after, 30 minutes after and 60 minutes after, Pulse period significantly increased in 30 minutes after and 60 minutes after. 2. T4 didn't significantly changed, T-T4 significantly increased in immediately after, 30 minutes after and 60 minutes after. T4/T, T4/(T-T4), T1/T, T5/T significantly decreased in immediately after, 30 minutes after and 60 minutes after. (T-T4)/T significantly decreased in immediately after, 30 minutes after and 60 minutes after. T5 significantly increased in 30 minutes after and 60 minutes after. 3. Modulus of elasticity significantly decreased in left Cun 60 minutes after, significantly increased in left Chi 30 minutes after. 4. Variance of Amplitude significantly increased in right Guan 60 minutes after. 5. Area of pulse significantly increased in left Cun 60 minutes after, left Chi 30 minutes after and right Cun 60 minutes after. Systolic pulse area significantly decreased left Chi 30 minutes after, right Cun immediately after, 30 minutes after and 60 minutes after, right Guan in immediately after. 6. Energy/min significantly decreased in left Chi 60 minutes after and right Cun immediately after. EIx significantly decreased in right Cun immediately after. 7. In both sides Cun, Guan, Chi wrist pulse, a lot of significant changes in right Cun and left Chi appeared, and then followed by the left Cun, right Guan. Conclusion : This study analyzed that the correlation between LS acupuncture and radial pulse(cun, guan, chi) is considered to be meaningful, hereafter clinical studies on this are needed.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.12
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• pp.8455-8461
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• 2015

In this paper, we propose new search algorithms using SVD(Singular Value Decomposition) for fast search of Raman spectrum. In the proposed algorithms, small number of the eigen vectors obtained by SVD are chosen in accordance with their respective significance to achieve computation reduction. By introducing pilot test, we exclude large number of data from search and then, we apply partial distance search(PDS) for further computation reduction. We prepared 14,032 kinds of chemical Raman spectrum as the library for comparisons. Experiments were carried out with 7 methods, that is Full Search, PDS, 1DMPS modified MPS for applying to 1-dimensional space data with PDS(1DMPS+PDS), 1DMPS with PDS by using descending sorted variance of data(1DMPS Sort with Variance+PDS), 250-dimensional components of the SVD with PDS(250SVD+PDS) and proposed algorithms, PSP and PSSP. For exact comparison of computations, we compared the number of multiplications and additions required for each method. According to the experiments, PSSP algorithm shows 64.8% computation reduction when compared with 250SVD+PDS while PSP shows 157% computation reduction.

• Journal of the korean academy of Pediatric Dentistry
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• v.42 no.4
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• pp.312-318
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• 2015

The aim of this study was to investigate the possibility of the supernumerary teeth for the stem cell source in dentistry. The Real Time Quantitative Reverse Transcription Polymerase Chain Reaction (Real Time qRT-PCR) method was used to evaluate the differentiation toward the odontoblast of the supernumerary dental pulp stem cells (sDPSCs). Supernumerary dental pulp stem cells were obtained from 3 children (2 males and 1 female, age 7 to 9) diagnosed that the eruption of permanent teeth was disturbed by supernumerary teeth. The common genes for odontoblasts are alkaline phosphatase (ALP), osteocalcin (OC), osteonectin (ON), dentin matrix acidic phosphoprotein 1 (DMP-1), dentin sialophosphoprotein (DSPP). The sDPSCs were treated for 0 days, 8 days and 14 days with additives and then Real Time qRT-PCR was performed in intervals of 0 days, 8 days and 14 days. The alizarin-red solution staining was performed to visualize the stained color for the degree of calcification at 7 days, 14 days, 21 days and 28 days after treating additives to the sDPSCs. From the result of the Real Time qRT-PCR, the manifestation exhibit maximum value at 8 days after additive treatment and shifted to a decrease trend at 14 days. Alizarin-red solution staining exhibit light results at 7 days after staining and generalized dark result at 14 days. Consequently, in studies with sDPSCs, appropriate treatment time of additives for Real Time qRT-PCR is 8 days. Also, a suitable period of Alizarin-red solution staining is 14 days.

• Korean Journal of Environmental Agriculture
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• v.19 no.3
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• pp.228-233
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• 2000

This study was carried out to estimate the possibility on the removal of phenols and anilines, which were contained in pulp or dye waste water, and the reusability of plant materials, shepherd's purse and turnip. Most of phenols catalyzed with shepherd's purse were removed more than 90% in the presence of $H_2O_2$, and the removal was ranged from 53.1% for 2,6-DMP to more than 99% for 2,4,6-TCP when turnip was used as catalysts. The removal of anilines catalyzed with shepherd's purse was ranged from 42.2% for 2-CA to 78.7% for 3,4-DCA in the presence of $H_2O_2$, and in case of turnip, from 31.5% for 2-CA to 90.0 % for 2,4-DCA. The reuse of plant materials was proved to be possible for not only the batch method but also the continuous method. No decreasing removal was observed during 30 cycles in waster water contaminated with 100ppm of 2,4-DCP. However, it was observed that the removal was decreased with increasing the number of cycles in higher concentration of 2,4-DCP(800ppm). Therefore, it could be suggested that the number of reusable cycles depends on the initial concentration of substrates.

• Journal of Food Hygiene and Safety
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• v.16 no.2
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• pp.125-132
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• 2001

The present study was conducted to investigate biological degradability of phosphamidon and profenfos. In the biodegradation test of two pesticides by the modified river die-away method from May 20 to July 29, 1999, the biodegradation rate was determined in Nakdong (A) and Kumho(B) River. The residual percentages of phosphamidon were 74.9%, 68.8% and 62.7% in control, A and B samples 7 days after applicaton, respectively. Biodegradation constants and half-lives of phosphamidon were 25.1%, 21.9% and 11.9% in control, A and B samples 7 days after application. Biodegradation constants and half-lives of profenofos were 0.0005 and 58.4 days in A, 0.0013 and 21.6 days in B, respectively. The biodegradation rates of phosphamidon and profenofos were higher in the Kumho River (B) than in the Nackdong River(A). The strains of microorganisms for the degradation of phosphamidon and profenofos were identified as Klebsiella pneumoniae, Aeromonas hydrophila and Acinetobacter calcoaceticus, all Gram-negative bacteria. In order to identify biodegradate products, the extracts of cultivates were analyzed by GC/MS. The mass spectra of biodegradate roducts of phosphamidon were at m/z 153 and 149, those of the profenofos were at m/z 208 and 240, respectively. It was suggested that the biodegradate metabolites of phosphamidon were O, O-dimethyl phosphate(DMP) and N, N-diethylchloroacetamide, those of profenofos were 4-bromo-2-chlorophenol and O-ethyl-S-propyl phosphate.

• Journal of Food Hygiene and Safety
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• v.14 no.4
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• pp.339-345
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• 1999

The present study was performed to investigate photodegradation rate constants and degradation products of dichlorvos and methidathion by the USEPA method. The two pesticides were very stable in sunlight for 16 days from September 2 to 18, 1998 and humic acid had no sensitizing effect on the photolysis of each pesticide in sunlight. The photolysis rate was fast-est for methidathion, followed by dichlorvos in the presence of UV irradiation. Photodegradation rate constant and half-life of dichlorvos were 0.0208 and 33.3 min, respectively. Photodegradation rate constant and half-life of methidathion were 0.6789 and 1.0min, respectively. The two pesticides were degraded completely in the presence of UV irradiation and UV irradiation with TiO$_2$in about 3 hours. Therefore, it is suggested that UV treatment will be effective for the degradation of pesticides in the process of drinking water purification. In case of dichlorvos and methidathion, UV irradiation with TiO$_2$was more effective for degradation than W irradiation. In order to identify photolysis products, the extracts of degradation products were analyzed by GC/ MS. The mass spectrum of photolysis products of dichlorvos was at m/z 153, those of the photolysis of methidathion were at m/z 198 and 214, respectively. Photolysis products of dichlorvos was Ο, Ο-dimethyl phosphate(DMP), those of methidathion were Ο, Ο-dimethyl phosphorothioate(DMTP) and Ο, Ο-dimethyl phosphorodithioate (DMDTP).

• The Journal of Korean Academy of Prosthodontics
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• v.58 no.1
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• pp.58-66
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• 2020

Implant-Assisted Removable Partial Dentures (IARPDs) treatment is being performed in a fully edentulous patient using implant surveyed prosthesis as an abutment. Implant-supported prosthesis as an abutment of IARPDs is classified into screw-retained and cement-retained type according to the retention type, and each has advantages and disadvantages. The EZ crown system (Samwon DMP, Yangsan, Korea) has a cylinder combined with abutment, and the nickel-titanium spring in this cylinder provides a constant force on the zirconia ball to obtain retention in EZ crown system. In this patient, the natural abutment teeth of the mandibular overdenture was hopeless. We planned implant assisted removable partial denture using anterior implant surveyed prosthesis considering functional and esthetical rehabilitation, cost and patient's needs. When fabricating IARPDs using implant as abutment, we could compensate for the shortcomings of existing implant-supported prosthesis retention type and made the design of removable partial denture easy due to using EZ crown system.

• Analytical Science and Technology
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• v.25 no.5
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• pp.273-283
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• 2012

Phthalate plasticizers are regulated by RoHS, REACH and CPSC as hazardous substances. Responding to these international environmental restrictions, we developed ABS certified reference material (CRM) for determination of phthalate plasticizers such as DMP, DEP, DBP, BBP, DEHP and DnOP. The candidate material has been made with ABS resin widely used in electric and electronic products and 6 kinds of phthalate plasticizers. The making of the material involved a series of processes like extruding, cooling, pelletizing, and drying using twin screw extruder. Then it has been certified according to ISO Guide 35. Using isotope dilution-gas chromatography/mass spectrometry (ID-GC/MS), homogeneity, short-term stability, and long-term stability were evaluated. The certified values were determined by using primary reference material (PRM) of KRISS for traceability. From now on, we will provide ABS CRM to national and international companies and research institutes after certification as certified reference material and registering on COMAR (code of reference material).

• Journal of Life Science
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• v.24 no.1
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• pp.54-60
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• 2014

Aromatic hydrocarbons are toxic environmental pollutants that are detrimental to the ecosystem and human health. Among them, chlorotoluene and nitrotoluene are toxic to hydrobios and irritate the skin, eyes, and respiratory organs of humans. We herein report the development of recombinant microbial biosensors for cheap and rapid monitoring of chlorotoluene and nitrotoluene compounds. Plasmids were constructed by inserting the xylR regulatory gene for BTEX (benzene, toluene, ethylbenzene, and xylene) degradation into upstream of Po' (the DmpR activator promoter Po with the deletion of its own upstream activating sequences) or Pu (the cognate promoter of XylR)::lacZ (the ${\beta}$-galactosidase gene) and transformed into Escherichia coli $DH5{\alpha}$. In the presence of inducers, the biosensor cells immobilized in agarose developed a red color in 1-2 h due to the hydrolysis of chlorophenol red ${\beta}$-D-galactopyranoside (CPRG), a substrate of ${\beta}$-galactosidase that was expressed by the inducers. Among BTEX, high responses were specifically observed with o-, m-, p-chlorotoluene ($0.1{\mu}M-100 mM$) and o-, m-, p-nitrotoluene (0.1 mM-100 mM). Po' demonstrated higher responses than those with Pu. The biosensors immobilized in agarose showed good stability after 21 days' storage at $4^{\circ}C$, and responses in untreated wastewater spiked with chlorotoluene and nitrotoluene, suggesting they can be used to detect compounds in wastewater.

• Journal of dental hygiene science
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• v.13 no.3
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• pp.296-303
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• 2013

Lysyl oxidase (LOX), extracellular matrix enzyme, is catalyzing lysine-derived crosslinks in collagen and elastin. Recently, several LOX-like proteins (LOXL, LOXL2, LOXL3 and LOXL4) have been identified in human but their specific functions are still largely unknown. The purpose of this study was to evaluate the function of the LOX family genes during odontoblastic differentiation of human dental pulp (HDP) cells induced with odontogenic supplement (OS). The messenger RNA (mRNA) expression of LOX family genes and differentiation markers was assessed by reverse transcriptase polymerase chain reaction analysis (RT-PCR). The formation of mineralization nodules was evaluated by alrizarin red S staining. Amine oxidase activity of HDP cells was measured by peroxidase-coupled fluormetric assay. The expressions of differentiation markers, such as alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN), dentin matrix protein1 (DMP1), dentin sialophosphoprotein (DSPP) in HDP cells were increased after treatment with OS media. The LOX and LOXL mRNA expression were gradually increased in OS media, whereas LOX enzyme activities were markedly detected on day 7. The mRNA expression and LOX enzyme activity of collagen type I was very similar to the pattern of LOX gene. In this study, the expression of LOX and its isoforms, and activity of LOX were highly regulated during odontoblastic differentiation. Thus, these results suggest that LOX plays a key role in odontoblastic differentiation of HDP cells.