• Title/Summary/Keyword: DIN

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Molecular Characterization of Various Trichomonad Species Isolated from Humans and Related Mammals in Indonesia

  • Kamaruddin, Mudyawati;Tokoro, Masaharu;Rahman, Md. Moshiur;Arayama, Shunsuke;Hidayati, Anggi P.N.;Syafruddin, Din;Asih, Puji B.S.;Yoshikawa, Hisao;Kawahara, Ei
    • Parasites, Hosts and Diseases
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    • v.52 no.5
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    • pp.471-478
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    • 2014
  • Trichomonad species inhabit a variety of vertebrate hosts; however, their potential zoonotic transmission has not been clearly addressed, especially with regard to human infection. Twenty-one strains of trichomonads isolated from humans (5 isolates), pigs (6 isolates), rodents (6 isolates), a water buffalo (1 isolate), a cow (1 isolate), a goat (1 isolate), and a dog (1 isolate) were collected in Indonesia and molecularly characterized. The DNA sequences of the partial 18S small subunit ribosomal RNA (rRNA) gene or 5.8S rRNA gene locus with its flanking regions (internal transcribed spacer region, ITS1 and ITS2) were identified in various trichomonads; Simplicimonas sp., Hexamastix mitis, and Hypotrichomonas sp. from rodents, and Tetratrichomonas sp. and Trichomonas sp. from pigs. All of these species were not detected in humans, whereas Pentatrichomonas hominis was identified in humans, pigs, the dog, the water buffalo, the cow, and the goat. Even when using the high-resolution gene locus of the ITS regions, all P. hominis strains were genetically identical; thus zoonotic transmission between humans and these closely related mammals may be occurring in the area investigated. The detection of Simplicimonas sp. in rodents (Rattus exulans) and P. hominis in water buffalo in this study revealed newly recognized host adaptations and suggested the existence of remaining unrevealed ranges of hosts in the trichomonad species.

Studies on the Mechanical Activities of Rabbit Myometrium V. Effects of Acetylcholine, Oxytocin and Prostagla, din F2α on Cyclic Nucleotide Levels of Rabbit Whole Uterus (가토 척출 자궁근의 운동성에 관한 연구 V. Acetylcholine, PGF2α 및 Oxytocin의 자궁 수축기전에 관한 연구)

  • Lee, Chang-Eop;Kwun, Jong-Kuk;Lee, Joong-Sup;Yang, Il-Suk;Lee, Mun-Han
    • Korean Journal of Veterinary Research
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    • v.22 no.1
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    • pp.1-8
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    • 1982
  • The effect of acetylcholine, oxytocin and prostaglandin $F_{2{\alpha}}$ ($PGF_{2{\alpha}}$) on cyclic nucleotide levels in estrogen-primed rabbit whole uterus were studied in the presence and absence of 1-methyl-3-isobutyl xanthine (MIX), a phosphodiestrase inhibitor, and indomethacin, a prostagandin inhibitor. In the absence of MIX, acetylcholine increased guanosine 3', 5'-cyclic monophosphate (cGMP), but had no effect on adenosine 3', 5'-cyclic monophosphate (cAMP) levels. In contrast, oxytocin had no influence on cGMP, but decreased cAMP levels. $PGF_{2{\alpha}}$ increased cGMP and decreased cAMP levels. MIX increased both cAMP and cGMP levels. Oxytocin and $PGF_{2{\alpha}}$ further increased cGMP levels, indicating activation of guanylate cyclase activity. The ratio of cAMP/cGMP was decreased by uterine stinulants both in presence and absence of MIX. Indomethacin elevated cAMP and cGMP revels. The effects of uterine stimulants in the presence of indomethacin on cyclic nucleotide levels were varied from tissue to tisse. In general, oxytocin decreased cGMP and $PGF_{2{\alpha}}$ increased cAMP/cGMP levels, but the effects were statisically nonsignicficant. The cAMP/cGMP ratio was increased by uterine stimulant in the presence of indomethacin. In conclusion, uterine stimulants eased cAMP/cGMP ratio which indicates that the uterine stimulants have opposing effects on adenylate cyclase and guanylate cyclase activities. The endometrium plays a role in the regulation of cyclic nucleotide levels and uterine contraction by means of PG synthesis. Indomethacin has an unknown activities besides both of PG synthetase and phosphodiesterase inhibitions.

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Near IR Luminescence Properties of Er-doped Sol-Gel Films (Er이 도핑된 졸-겔 코팅막의 발광특성)

  • Lim, Mi-Ae;Seok, Sang-Il;Kim, Ju-Hyeun;Ahn, Bok-Yeop;Kwon, Jeong-Oh
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2003.11a
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    • pp.136-136
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    • 2003
  • In fiber optic networks, system size and cost can be significantly reduced by development of optical components through planar optical waveguides. One important step to realize the compact optical devices is to develop planar optical amplifier to compensate the losses in splitter or other components. Planar amplifier provides optical gain in devices less than tens of centimeters long, as opposed to fiber amplifiers with lengths of typically tens of meters. To achieve the same amount of gain between the planar and fiber optical amplifier, much higher Er doping levels responsible for the gain than in the fiber amplifier are required due to the reduced path length. These doping must be done without the loss of homogeniety to minimize Er ion-ion interactions which reduce gain by co-operative upconversion. Sol-gel process has become a feasible method to allow the incorporation of Er ion concentrations higher than conventional glass melting methods. In this work, Er-doped $SiO_2$-A1$_2$ $O_3$ films were prepared by two different method via sol -Eel process. Tetraethylorthosilicate(TEOS)/aluminum secondary butoxide [Al (OC$_4$ $H_{9}$)$_3$], methacryloxypropylcnethoxysaane(MPTS)/aluminum secondary butofde [Al(OC$_4$ $H_{9}$)$_3$] systems were used as starting materials for hosting Er ions. Er-doped $SiO_2$-A1$_2$ $O_3$ films obtahed after heat-treating, coatings on Si substrate were characterized by X-ray din action, FT-IR, and N-IR fluorescence spectroscopy. The luminescence properties for two different processing procedure will be compared and discussed from peak intensity and life time.

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The Characteristics of the Output Voltage Ferroelectrics for High Voltages Pulse Generators (고전압 펄스 발생기를 위한 강유전체의 전압 출력 특성)

  • Jang, Dong-Gwan;Choi, Sun-Ho;Hwang, Sunl-Mook;Huh, Chang-Su
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.62 no.10
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    • pp.1408-1412
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    • 2013
  • High power pulse generating technology is to accumulate the energy for relatively long and then to create a strong force by emitting the energy very fast. High power pulse generating technology has recently been using in various fields like environments, industry, research, military and so on. Numerous studies about high power pulse generators have already been performed and commercialized in various conditions. However, in aspect of their size and weight, it is hard to carry the generators which currently have been developed. For these reasons, din nations like America or Russia, the researches have been performed for Ferroelectric Generators(FEG), which have relatively simple structure and are economical. To realize the ferroelectric generator, in this study, we selected the PZTs which have different physical properties respectively, and then shocked them using explosives. The PZT samples with volumes of $0.31{\sim}0.94cm^3$ were depolarized by shocked and produced the waveform that have peak voltages of 4.28 ~ 15kV. The lowest relative permittivity sample generated much higher peak voltage. And sudden voltage drops which seem to be caused by dielectric breakdown were observed in some experiments using low young's modulus samples. Also, increase in thickness led to increase in peak voltage, but the ratio of the voltage rise did not reach the ration of the thickness increase.

Study on Food Intake and Food Consumption Pattern of Adults as a Part of Total Diet Study (총식이조사를 위한 식품의 소비형태 및 식품섭취량 조사 연구)

  • 계승희
    • Journal of Nutrition and Health
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    • v.30 no.1
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    • pp.61-74
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    • 1997
  • A dietary intake survey in 2 major city area was conducted as a part of the study which determined the level of exposure of people to harmful substances in foodstuff. A total of 800 randomly selected men and women aged 20-49 yr and living in the cities of Seoul and Taegu were invited to participate in the survey. All subjects were interviewed by trained dietitians to answer for demorgraphic characteristics and dietary intake for 1 day by 24hr recall method, and asked to record what day ate for following 2 more days by themselves. Results were analyzed and presented for the amount and frequency of food intake by area, sex body weight. Food consumed most by adults resifing in these 2 city area was rice and followed by kimchi in the amount of 225.7g/persn/day and 85.0g/person/day, respectively. In the list of foods consumed most frequently, items used for condiments were included in addition to rice and kimchi. And the total daily intake of food per person was summed up to be 1, 534g for men and 1, 304g for women. Considerable part of this sexual difference in total intake was originated from the difference in beverage consumption including alcoholic beverages and the higher intake of certain foodstuff due to higher body weight of men. Intake data were presented din terms of per capita and per kg body weight, and compared among adults in different categories of body weight, also. The data gathered here, food intake by individuals, reflect our own dietary culture in terms of food assortment and relative contribution of each food item to total food intake include the consumption of "extreme" eaters might be found useful also especially for the sake of food safety such as risk assessment using ADI and PTWI for contaminants in the food supply.od supply.

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A Study on Improvement of the Standards for the Manoeuvring space at Doors (출입문 측면 활동공간 설치기준 개선에 관한 연구)

  • Shin, Dong-Hong;Park, Kwang-Jae
    • Journal of The Korea Institute of Healthcare Architecture
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    • v.25 no.4
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    • pp.71-79
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    • 2019
  • Purpose: The manoeuvring space at doors must be secured for users of walking aids, wheelchair users, stroller users, luggage carriers, etc., so clear criteria must be established. The korean standards for the manoeuvring space at doors need to be improved to present clear criteria depending on the type of entrance and the direction of opening and closing. Method: Korean standard, International Standard(ISO 21542, 2011), German Standard(DIN 18040-1, 2010), Austrian Standard(OENORM B 1600, 2017) and Swiss Standard(Norm SIA 500, 2009) were investigated and analyzed. A comprehensive improvement of the criteria for the manoeuvring space at doors is proposed. Results: The manoeuvring space at doors, which is essential for the disabled to use the door, shall be installed with a comprehensive consideration of the width of doors, the Circulation space, the direction of access, and the method of opening and closing of the door. Korean standards, such as the width of doors, the Circulation space, and the manoeuvring space at doors, are different from international and international standards, which require improvement of standards. The criteria for the manoeuvring space at doors shall be clearly improved so as not to be misinterpreted during the installation of doors and the BF-certification. Implication: The criteria for the installation of doors shall be presented as a comprehensive alternative through continued study of details. In addition, improvements in standards that reflect changes in international standards should be made.

Environmental Studies in the Lower Part of the Han River -VII. Long Term Variations and Prospect of the Phytoplankton Community- (한강하류의 환경학적 연구 -VII. 식물플랑크톤군집의 장기간 변화와 전망-)

  • Lee, Jin-Hwan;Jung, Seung-Won
    • ALGAE
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    • v.19 no.4
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    • pp.321-327
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    • 2004
  • The literature review on the dynamics of the phytoplankton communities in terms of species composition, standing crops, abundant species and dominant species in the lower part of the Han River from 1940s to 2000s was conducted for the prospective prediction of their succession patterns. Total of 326 taxa were identified and they belonged to 47 blue-green algae, 139 green algae, 12 euglenoids, 126 diatoms, 6 din flagellates and 2 silicoflagellates. Composition of phytoplankton communities were 83.6% diatoms, 10.5% blue-green algae and 5.3% green algae in the middle of 1960s, whereas those were 43.2% diatoms, 40.7% green algae and 13.6% blue-green algae in the 1990s. Before 1990s, Synedra ulna, Melosira varians, Cymbella tumida, Synedra acus, Cymbella ventricosa, Navicula cryptocephala, Nitzschia palea, Aulacoseira granulata, Gomphonema parvulum and Cymbella affinis were most frequent, while those after 1990 were Asterionella formosa, Asterionella gracillima, Aulacoseira granulate, Aulacoseira granulata var. angustissima, Chlorella vulgare, Fragilaria crotonensis and Synedra ulna. Phytoplankton blooms were frequent from winter to the late spring and rare in summer due to heavy rain and discharge. Seasonal variations of the dominant species were fairly obvious; Asterionella gracillima and Aulacoseira granulata in spring, Aulacoseira granulate and Aulacoseira granulate var. angustissima in summer and autumn, Asterionella gracillima and Stephan discus hantzschii in winter. Recently blue-green algae, Microcystis, Aphanocapsa, Dactylococcopsis have been more abundant than those of the previous reports. Based on the current situations, Stephan discus hantzschii f. tennis, Asterionella gracillima, Aulacoseira granulate and blue-green algae will be more abundant and blooms of those species will be more frequent.

New Haplotypes of the ATP Synthase Subunit 6 Gene of Mitochondrial DNA are Associated with Acute Lymphoblastic Leukemia in Saudi Arabia

  • Yacoub, Haitham Ahmed;Mahmoud, Wael Mahmoud;El-Baz, Hatim Alaa-Eldeen El-Din;Eid, Ola Mohamed;El-Fayoumi, Refaat Ibrahim;Mahmoud, Maged Mostafa;Harakeh, Steve;Abuzinadah, Osama H.A.
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.23
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    • pp.10433-10438
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    • 2015
  • Background: Acute lymphoblastic leukemia (ALL) is the most common cancer diagnosed in children and represents approximately 25% of cancer diagnoses among those younger than 15 years of age. Aim and Objectives: This study investigated substitutions in the ATP synthase subunit 6 gene of mitochondrial DNA (mtDNA) as a potential diagnostic biomarker for early detection and diagnosis of acute lymphoblastic leukemia. Based on mtDNA from 23 subjects diagnosed with acute lymphoblastic leukemia, approximately 465 bp of the ATP synthase subunit 6 gene were amplified and sequenced. Results: The sequencing revealed thirty-one mutations at 14 locations in ATP synthase subunit 6 of mtDNA in the ALL subjects. All were identified as single nucleotide polymorphisms (SNPs) with a homoplasmic pattern. The mutations were distributed between males and females. Novel haplotypes were identified in this investigation: haplotype (G) was recorded in 34% in diagnosed subjects; the second haplotype was (C) with frequency of 13% in ALL subjects. Neither of these were observed in control samples. Conclusions: These haplotypes were identified for the first time in acute lymphoblastic leukemia patients. Five mutations able to change amino acid synthesis for the ATP synthase subunit 6 were associated with acute lymphoblastic leukemia. This investigation could be used to provide an overview of incidence frequency of acute lyphoblastic leukemia (ALL) in Saudi patients based on molecular events.

The Distribution of Nitrogen and the Decomposition Rate of Organic Nitrogen in the Youngsan River and the Sumjin River, Korea (영산강과 섬진강 수계의 질소 분포와 유기질소 분해속도)

  • Kim, Jihye;Kim, Bomchul;Shin, Myoungsun;Kim, Jaiku;Jung, Sungmin;Lee, Yunkyoung;Park, Juhyun
    • Journal of Korean Society on Water Environment
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    • v.25 no.1
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    • pp.142-150
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    • 2009
  • The distribution of organic nitrogen and its decomposition rate were studied in the Youngsan River and the Sumjin River system in Korea. Samples were conducted seasonally in June, August, December of 2006, and February of 2007. Collected samples were incubated for 20 days in a dark chamber ($20{\pm}1^{\circ}C$) and analyzed the changes of nitrogen form (particulate organic nitrogen, dissolved organic nitrogen, ammonia, nitrite, and nitrate). The mean total nitrogen (TN) concentration in the Youngsan River and the Sumjin River were $2.62mgN{\cdot}L^{-1}$ and $1.53mgN{\cdot}L^{-1}$, respectively. TN comprised of 65% DIN and 35% ON. The decomposition coefficients of organic nitrogen were also determined by two different fitting models. The decomposition rates of nitrogen species (TON, LPON, LDON, ${NH_4}^+$ and ${NO_2}^-$) ranged from 0.024 to $1.043day^{-1}$ in the Youngsan River and 0.008 to $0.693day^{-1}$ in the Sumjin River, respectively. The result of this study can give a guide to the selection of parameters in the calibration processes of water quality models.

Finite Element Analyses on Ultimate Compressive Strength of Longitudinally Stiffened Polygonal Sections (종방향 보강재로 보강된 다각형 단면 기둥구조의 극한강도 해석)

  • Choi, Byung Ho;Park, Seong Mi;Hwang, Min Oh
    • 한국방재학회:학술대회논문집
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    • 2011.02a
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    • pp.40-40
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    • 2011
  • 현재 일반적으로 활용되고 있는 원통형 쉘구조로 이루어진 타워구조의 대형화가 추진되면서 제작, 운반 편의성, 단면효율성, 경제성 제고를 위해 다각형단면 기둥구조물의 활용이 대두되고 있다. 하지만 다각형 단면 기둥구조의 극한강도에 대한 자료가 충분치 않고 관련 기준이나 지침이 명확히 제시되고 있지 않은 실정이다. 본 연구에서는 원통형 쉘구조물을 다각형구조물로 대체하여 제작될 경우 축방향 압축에 대한 내하력 향상 효과를 수치해석적으로 검토해 보고자 한다. 해석모델은 지름 2m, 두께 20mm인 원형강관 프로토타입 풍력타워 구조를 참고로 하여 이에 내접하도록 결정한 6~12각형 단면 형상으로써 높이 10,000mm인 3차원 기둥모델을 구현하였고 유한요소프로그램인 ABAQUS를 이용하여 해석하였다. 각 subpanel의 중앙에 종방향 보강재를 설치하였을 때 국부좌굴에 대한 내하력 변화를 비교하기 위해 종방향보강재로 보강한 모델을 구성하여 비교 해석을 수행하였다. 종방향 보강재의 제원은 미국 SSRC 제안식을 기준으로 삼았다. 탄성좌굴해석을 통해 탄성좌굴모드 형상을, 비선형비탄성해석을 통해 최종파괴모드 및 극한강도를 얻었다. 보강 전 후의 탄성좌굴 해석 결과로부터 최소모드의 고유치 값을 비교하였다. 각 subpanel 단면 중심부에 한 개의 보강재를 설치한 경우 탄성좌굴강도가 4배 가량 증가하였다. 이로부터, 보강재(n=1) 설치에 따라 유효 폭두께비가 1/2로 감소하는 효과를 확인 할 수 있다. 비선형해석결과로부터 subpanel의 단면중심에 보강재를 설치한 경우 보강재가 위치한 곳에 고정점이 형성되어 이를 중심으로 국부 좌굴모드에 변화가 생기는 것이 확인되었다. 이러한 변화는 다각형 단면 기둥구조의 내하력 성능, 즉 국부좌굴강도에 영향을 준다. 충분한 강성을 갖는 종방향 보강재가 설치된 경우, 극한상태에서도 유효폭두께비가 줄어드는 것과 같은 강도 향상 효과를 확인할 수 있다. 이러한 사실은 각 해석결과 극한강도를 DIN code, Migita와 Fukumoto의 제안식, SSRC 설계제안식 등과의 비교를 통해 확인할 수 있었다.

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