• Title/Summary/Keyword: DIN

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Genes involved in leaf senescence and regulation of their expression

  • Watanabe, Akira;Fujiki, Yuki;Yoshikawa, Yoko;Biswall, Basanti;Ito, Masaki
    • Proceedings of the Botanical Society of Korea Conference
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    • 1999.07a
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    • pp.63-67
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    • 1999
  • We have isolated more than a dozen cDNA clones corresponding to genes that were expressed in Arabidopsis leaves when they were kept in the dark. The nucleotide sequence analysis showed that some of the clones encoded proteins with significant homology to $\beta$-glucosidase (din2), branched-chain $\alpha$-keto acid dehydrogenase subunit E1$\beta$(din3), and another subunit E2 (din4), yeast RAD23 (din5), asparagine synthetase (din6), pre-mRNA splicing factor SRp35 (din7), phosphomannose isomerase (din9), seed imbibition protein (din10), and 2-oxoacid-dependent oxidase (din11). Accumulation of transcripts from din3,4,6 and 10 occurred rapidly after the plants were transferred to darkness. Transcripts from din2,9, and 11 could be detected only after 24 h of dark treatment. Inhibition of photo-synthesis by DCMU strongly induced the accumulation of transcripts from those genes, and application of sucrose to detached leaves suppressed the accumulation both in the dark and by DCMU. These observations indicate that expression of the genes is caused by sugar starvation resulted from the cessation of photosynthesis. We further showed that din2-encoded protein also accumulated in senescing leaves. Given these results, possible roles of din genes in leaves in the dark and senescing leaves are discussed.

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Distribution of Nitrogen Components in Seawater Overlying the Gomso Tidal Flat (곰소만 조간대 해수 내 질소 성분의 시공간적인 분포)

  • 양재삼;김기현;김영태
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.8 no.3
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    • pp.251-261
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    • 2003
  • As a part of an on-going project investigating flux of materials in Gomso Tidal Flat, we have monitored temporal and spatial distribution of nitrogen components(TN, PON, DON, DIN) and have sought the relationships with the freshwater input(tidal range, salinity), the biological activities(chlorophyll-${\alpha}$, TP, DIP, silicate) and the resuspended bottom sediment in seawater(SPM) from 1999 to 2000. TN in seawater was 39.05 $\mu\textrm{m}$ol 1$\^$-1/ (31.03∼42.93 $\mu\textrm{m}$ol 1$\^$-1/) without any statistical difference(p<0.05) between the studied periods. Organic nitrogen (DON and PON) occupied 75%, 95%, 73%, and 75% in April, August, September and November, respectively. DON and PON have been found within the narrow concentration ranges of 11.30∼16.38 $\mu\textrm{m}$ol 1$\^$-1/ and 13.16∼20.04 $\mu\textrm{m}$ol 1$\^$-1/ in spite of severe environmental differences through the studied periods. Dissolved fractions of nitrogen(DON and DIN) occupied 53∼65% of TN. Only DIN varied with an evident temporal variability: low concentrations(1.325∼1.616 $\mu\textrm{m}$ol 1$\^$-1/) in August and high enrichment(8.377∼14.65 $\mu\textrm{m}$ol 1$\^$-1/) in September. High consumption rate of DIN by phytoplankton and a long-lasted drought probably induced such low concentration of DIN in August. Eventually heavy precipitation probably introduced plenty of new nitrogen sources into Gomso Bay in September. The portion of PON, DON and DIN in the total nitrogen was 40%, 38% and 22%, respectively. Their contents were in the order of DON>PON>DIN for the year round except PON>DON>DIN only in September. The highest DON portion in August probably due to the active microbial decomposition of organic material in summer. Only in April, some evident negative correlations have been found between chlorophyll-${\alpha}$ and DIN mostly nitrate(-0.64, p<0.01), phosphate(-0.46, p<0.01) and silicate(-0.55, p<0.01). The Si(OH)$_4$/DIN/DIP ratios in the water column suggests the limitation of DIN for the growth of phytoplankton during the dry summer in Gomso Bay, which was the case of August in this work. Even with some difference between the studied periods, the primary factors on the distribution of nitrogen components in seawater overlying the Gomso Tidal Flat have been the tidal range and the freshwater input, but the additional variations were due to the biological activities.

Application of 2-pass DInSAR to Improve DEM Precision (DEM 정밀도 향상을 위한 2-pass DInSAR 방법의 적용)

  • 윤근원;김상완;민경덕;원중선
    • Korean Journal of Remote Sensing
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    • v.17 no.3
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    • pp.231-242
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    • 2001
  • In 2-pass differential SAR interferometry(DInSAR), the topographic phase signature can be removed by using a digital elevation model(DEM) to isolate the contribution of deformation from interferometric phase. This method has an advantage of no unwrapping process, but applicability is limited by precision of the DEM used. The residual phase in 2-pass differential interferogram accounts for error of DEM used in the processing provided that no actual deformation exits. The objective of this paper is a preliminary study to improve DEM precision using low precision DEM and 2-pass DInSAR technique, and we applied the 2-pass DInSAR technique to Asan area. ERS-1/2 tandem complex images and DTED level 0 DEM were used for DInSAR, and the precision of resulting DEM was estimated by a 1:25,000 digital map. The input DEM can be improved by simply adding the DInSAR output to the original low precision DEM. The absolute altitude error of the improved DEM is 9.7m, which is about the half to that of the original DTED level 0 data. And absolute altitude error of the improved DEM is better than that from InSAR technique, 15.8m. This approach has an advantage over the InSAR technique in efficiently reducing layover effects over steep slope region. This study demonstrates that 2-pass DInSAR can also be used to improve DEM precision.

Dominance and Survival Strategy of Toxic Dinoflagellate Alexandrium tamarense and Alexandium catenella Under Dissolved Inorganic Nitrogen-limited Conditions (용존태 무기질소 제한 하에서 유독와편모조류 Alexandrium tamarense와 Alexandium catenella의 생존과 우점화 전략)

  • Kwon, Hyeong Kyu;Park, Ji A;Yang, Han-Soeb;Oh, Seok Jin
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.16 no.1
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    • pp.25-35
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    • 2013
  • Dominance and survival strategy of toxic dinoflagellate Alexandrium tamarense and A. catenella under the dissolved inorganic nitrogen (DIN) limited conditions were examined in the laboratory and field observations. In Masan Bay, DIN was limiting factor for growth of phytoplankton during spring to early summer when Alexandrium spp. have been observed. They have a disadvantageous position compared with diatoms because Ks of nitrate calculated from growth kinetics experiment of A. tamarense and A. catenella was higher than diatoms. However, A. tamarense and A. catenella were able to grow using dissolved organic nitrogen (DON) compounds such as urea and amino acids as well as DIN. Therefore, DON utilization of A. tamarense, A. catenella might contribute to not only their population growth but also dominance and interspecific competition in the DIN-limited conditions in Masan Bay.

Ground Subsidence Estimation in a Coastal Reclaimed Land Using JERS-1 L-band SAR Interferometry (JERS-1 L-band SAR Interferometry 를 이용한 연안매립지 지반침하 관측)

  • 김상완;이창욱;원중선
    • Economic and Environmental Geology
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    • v.35 no.5
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    • pp.465-478
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    • 2002
  • We measured subsidences occurred in a coastal reclaimed land, Noksan industrial complex, from May 2, 1996 to February 21, 1998, using 5 interferograms of JERS-1 L-band SAR. SAR with a spatial resolution of about 16 m can detect the two-dimensional distribution of subsidence that is difficult to be estimated from in situ measurements. Accuracy of the subsidences estimated by 2-pass DInSAR was evaluated using the measurements of Magnetic Probe Extensometer (accuracy of :${\pm}$1 mm) installed at 42 stations. DInSAR measurements were well correlated with the field measurements showing an average correlation coefficient of 0.77. The correlation coefficient was further improved to be 0.87 (with RMSE of 1.44 cm) when only highly coherenced (>0.5) pixels were used. The slope of regression line was 1.04, very close to the unit value. In short, DInSAR measurements have a good linear relation with field measurements so that we can effectively detect a subsidence in the coastal reclaimed area especially using pixels of high coherence (>0.5). The maximum accumulated subsidence was about 60 cm in the study area, while the subsidence in the northern and south western areas were less than 20 cm. The resuts show that DInSAR is extremely useful for geotechnical applications as well as observation of natural deformation.

Evaluation of the ETRmax in Microalgae Using the PHYTO-PAM Fluorometer (광합성 측정기를 이용한 미세조류의 광합성 효율 측정)

  • Cho, Eun-Seob;Lee, Pil-Yong;Oh, Hyun-Ju;Choi, Yoon-Seok;Choi, Yang-Ho;Lee, Sam-Geun
    • Journal of Environmental Science International
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    • v.15 no.8
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    • pp.727-735
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    • 2006
  • In this study, the PHYTO-PAM-fluorometric method was used to evaluate the ETR$_{max}$ in terms of sensitivity to DIN/DIP against 14 microalgae: Prorocentrum micans, Heterocapsa triquetra, Gymnodinium impudicum, Cymnodinium catenatum, Amphidinium caterae, Chlorella vulgaris, Chroococcus minutus, Microcystis aeruginosa, Chlorella ellipsoidea, Nannochloris oculata, Oocystis lacustris, Chroomonas salina, Gloeocystis gigas, and Prymnessium parvum. We found that P. micans, H. triquetra, and A. caterae exposed to the maximum level of DIN/DIP were significantly smaller in the ETR$_{max}$ than that of the minimum and moderate mixture. Unlikely the ETR$_{max}$, the initial slope alpha was not significantly different at the level of 60 DIN/DIP. In G. catenatum, the moderate levels of 15 and 20 in DIN/DIP were found to be significantly different from the ETR$_{max}$ at Chl-Ch4. Gymnodinium impudicum had a higher value than that of the ETR$_{max}$ than that of dinoflagellates used in this study, ranging from 306.1 (Ch4, DIN/DIP: 10) to 520.1 (Ch4, DIN/DIP: 30). The ETR$_{max}$ value obtained from other microalgae was similar to C. impudicum at any of the ratios of DIN/DIP and channels. Consequently, the influence of offshore water current assures us of the suppression of photosynthesis and electron transport rate in dinoflagellates. Gymnodinium impudicum has not been researched in the area of red tides in Korea, but it will be enough to creat the massive algal blooms in the future because of higher potential photochemical availability.

First Record of an Ectoparasitic Dinoflagellate, Oodinium inlandicum (Dinophyta) Infecting a Chaetognath, Sagitta crassa from the Korean Coasts

  • Horiguchi, Takeo;Harada, Ai;Ohtsuka, Susumu;Soh, Ho-Young;Yoon, Yang-Ho
    • ALGAE
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    • v.19 no.3
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    • pp.201-205
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    • 2004
  • An ectoparasitic din flagellate infesting plank tonic chaetognath, Sagitta crassa Tokioka was found, for the first time, from Korean coasts. In order to identify the species, we investigated detailed morphology of the din flagellate using Nomarski interference optics as well as epifluorescent microscopes. The parasitic din flagellate consists of an oval to rod-shaped cell with a peduncle, by which the organism attaches to the host. The cell is covered with polygonal thecal plates. The nucleus displays two different shapes according to cell cycle stages: in young trophont the nucleus is elongated and shows typical din flagellate nucleus (dinokaryon), while in matured trophont, the nucleus is dome-shaped and non-dinokaryotic. The peduncle is variable in length and is ornamented with the longitudinal striations. All these characteristics point to identity that the ectoparasitic din flagellate infecting Sagitta crassa in Korean coasts is Oodinium inlandicum Horiguchi et Ohtsuka, originally described from the Seto Inland Sea of Japan. Relationship between prevalence and host sizes differed from those in Japan.

Land Surface Soil Moisture Effect on DInSAR

  • Lee C.W.;Kim S.W.;Won J.S.
    • Proceedings of the KSRS Conference
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    • 2004.10a
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    • pp.174-177
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    • 2004
  • Differential interferometric phases from JERS-1 L­band data sets show spatial variation of path-length ranging from a few mm to several cm. The variation may be caused by changes in soil moisture contents, i.e. variation of penetration depth and the swelling of soils. Although the amount of total effect caused by soil moisture is not measurable, it is clear that the soil moisture according to precipitation is another factor to be considered in DInSAR analysis. We also discuss DInSAR characteristics in a rice paddy according to irrigation conditions, and discrimination of hydrological features such as stream channels and watershed boundaries by applying DInSAR technique.

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Feasibility Studies of DInSAR in the Northeastern Kyungsang Basin, Korea

  • Lee, C.W.;Kim, S.W.;Jung, H.C.;Won, J.S.
    • Proceedings of the KSRS Conference
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    • 2003.11a
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    • pp.1070-1072
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    • 2003
  • This study focuses on examing the feasibility of differential SAR interferometry (DInSAR) in the Northeastern Kyungsang Basin, Korea. Major faults in the Kyungsang Basin such as Yangsan fault, Dongrae fault, and Ulsan fault had developed during Cretaceous, and the activeness of these faults is still controversial in Korean geology community. We attempt to measure displacements in the study area by applying DInSAR techniques to JERS-1 SAR data sets. Some surface displacements are recognized by DInSAR method at Young-il Bay in which the POSCO Company locates, although the displacements may not be directly associated with geologic structures. We also discuss atmospheric effects for the techniques used.

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The Importance of Nitrogen Release and Denitrification in Sediment to the Nitrogen Budget in Hiroshima Bay

  • KIM Do-Hee;MATSUDA Osamu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.29 no.6
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    • pp.779-786
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    • 1996
  • The main purpose of this study was to estimate the role of dissolved inorganic nitrogen (DIN) released from sediment and denitrification process in sediment on the nitrogen budget of Hiroshima Bay by means of collecting data on distributions and budgets of nitrogen and phosphorus in the bay, DIN fluxes across sediment-water interface and denitrification rates in the sediments of the same area. The TN : TP and DIN:DIP atomic ratios of the discharged freshwater were about 26 and 21, respectively. The standing stocks in the seawater of the TN : TP atomic ratio varied from 8 to 14 with an annual mean value of 11, while the DIN : DIP atomic ratio varied from 10 to 15 with an annual mean value of 12 in the bay. The residence time of nitrogen and phosphorus were estimated to be about 109 days and 200 days in the bay, respectively. The proportion of DIN released from sediment and denitrification rate to the loading of total nitrogen into Hiroshima Bay were $45\%\;(37\~82\%)\;and\;13\%(0.0\~37\%)$, respectively, and the amount of nitrogen through denitrification process was 6.5 times larger than the outflow of nitrogen from the bay. The results show that DIN released from sediment and denitrification process in sediment play important roles on the nitrogen budget in Hiroshima Bay.

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