DOI QR코드

DOI QR Code

Evaluation of the ETRmax in Microalgae Using the PHYTO-PAM Fluorometer

광합성 측정기를 이용한 미세조류의 광합성 효율 측정

  • Published : 2006.08.01

Abstract

In this study, the PHYTO-PAM-fluorometric method was used to evaluate the ETR$_{max}$ in terms of sensitivity to DIN/DIP against 14 microalgae: Prorocentrum micans, Heterocapsa triquetra, Gymnodinium impudicum, Cymnodinium catenatum, Amphidinium caterae, Chlorella vulgaris, Chroococcus minutus, Microcystis aeruginosa, Chlorella ellipsoidea, Nannochloris oculata, Oocystis lacustris, Chroomonas salina, Gloeocystis gigas, and Prymnessium parvum. We found that P. micans, H. triquetra, and A. caterae exposed to the maximum level of DIN/DIP were significantly smaller in the ETR$_{max}$ than that of the minimum and moderate mixture. Unlikely the ETR$_{max}$, the initial slope alpha was not significantly different at the level of 60 DIN/DIP. In G. catenatum, the moderate levels of 15 and 20 in DIN/DIP were found to be significantly different from the ETR$_{max}$ at Chl-Ch4. Gymnodinium impudicum had a higher value than that of the ETR$_{max}$ than that of dinoflagellates used in this study, ranging from 306.1 (Ch4, DIN/DIP: 10) to 520.1 (Ch4, DIN/DIP: 30). The ETR$_{max}$ value obtained from other microalgae was similar to C. impudicum at any of the ratios of DIN/DIP and channels. Consequently, the influence of offshore water current assures us of the suppression of photosynthesis and electron transport rate in dinoflagellates. Gymnodinium impudicum has not been researched in the area of red tides in Korea, but it will be enough to creat the massive algal blooms in the future because of higher potential photochemical availability.

Keywords

References

  1. Clayton, R. K., 1980, Photosynthesis: Physical mechanim and chemical patterns, Cambridge University Press, Cambridge, England, 215pp
  2. Douglas, S. E., 1998, Plastid evolution: origins, diversity, trends. Curr, Opin. Genet. Dev., 8, 655-661 https://doi.org/10.1016/S0959-437X(98)80033-6
  3. Lichenthaler, H. H., 1988, Application of chlorophyll fluorescence, Dordrecht, Kluwer Acad. Pub., 384pp
  4. Krause, G. and E. Weis, 1991, Chlorophyll fluorescence and photosynthesis: The basics, Annu. Rev. Plant Phys., 42, 313-349 https://doi.org/10.1146/annurev.pp.42.060191.001525
  5. Schreiber, U., 1986, Detection of rapid induction kinetics with a new type of high frequency modulated chlorophyll flurometer, Photosynth. Res., 9, 261-272 https://doi.org/10.1007/BF00029749
  6. Schreiber, U., W. Bilger and C. Neubauer, 1994, Chlorophyll fluorescence a non-intrusive indicator for rapid assessment of in vivo photosynthesis, Ecol. Stud., 100, 47-70
  7. Schreiber, U. and W. Biler, 1993, Progress in chlorophyll fluorescence research: major developments during the past years in retrospect, Progr. Botany, 54, 1510-173
  8. Gross, E. M., H. Meyer and G. Schilling, 1996, Release and ecological impact of hydrolysable polyphenols in Myriophyllum spicatum, Phytochemistry, 41, 133-138 https://doi.org/10.1016/0031-9422(95)00598-6
  9. Schreiber, D., R. Gademann, P. Bird, P. J. Ralph, A. W. D. Larkum and M. Kuhl, 2002, Apparent light requirement for activation of photosynthesis upon rehydration of desiccated beachrock microbial mats, J. Phycol., 38, 125-134 https://doi.org/10.1046/j.1529-8817.2002.01103.x
  10. Michael, R., L. Mckay, G. S. Bullerjahn, D. Porta, E. T. Brown, R. M. Sherrell, T. M. Smukta, R. W. Sterner, M. R. Twiss and S. W. Wilhelm, 2004, Consideration of the bioavailability of iron in the North Americal Great Lakes: Development of novel approaches toward understanding iron biogeochemistry, Aqua. Eco. Heal. Man., 7, 475-490 https://doi.org/10.1080/14634980490513364
  11. Hakanson, L., J. M. Malmaeus, D. Bodemer and V. Gerhardt, 2003, Coefficients of variation for chlorophyll, green algae, diatoms, crytophytes and blue-grees in rivers as a basis for predictive modelling and aquatic management, Ecol. Model., 169, 179-196 https://doi.org/10.1016/S0304-3800(03)00269-2
  12. Deterrnann, S., J. M. Lobbes, R. Reuter and J. Rullkotter, 1998, Ultraviolet fluorescence excitation and emission spectroscopy of marine algae and bacteria, Mar. Chemi., 62, 137-156 https://doi.org/10.1016/S0304-4203(98)00026-7
  13. Lee, S. W., 1996, An outline of oecanophysics, Jipmundang Press, Seoul, 310pp
  14. Goldman, J. C. and P. M. Gilibert, 1983, Kinetics of inorganic nitrogen uptake by phytoplankton, In: Nitrogen in the marine environment, Academic Press, New York, London, Paris, pp.233-274
  15. Woitke, P., C. D. Martin, S. Nicklisch and J. G. Kohl, 1994, HPLC determination of lipophilic photosynthetic pigments in algal cultures and lake water samples using a non-endcapped C 18-RP column, Fres. J. Anal. Chem., 348, 762-768 https://doi.org/10.1007/BF00323701
  16. Lee, C. K., H. C. Kim, S. G. Lee, C. S. Jung, H. G. Kim and W. A. Lim, 2001, Abundance of harmful algae, Cochlodinium polykrikoides, Gyrodinium impudicum, Gymnodinium catenatum in the coastal area of South Sea of Korea and their effects of temperature, salinity, irradiance and nutrient on the growth in culture, J. Kor. Fish. Soc., 34, 536-544
  17. Kim, H. G., S. G. Lee and K. H. An, eds., 1997, Recent red tides in Korean coastal waters, Kudeok Publishing, Busan, 280pp