• Journal of the Korean Society of Food Science and Nutrition
• /
• 제43권2호
• /
• pp.318-322
• /
• 2014

5-Alpha reductase II, which converts testosterone (T) to dihydrotestosterone (DHT), is a crucial enzyme II in benign prostatic hyperplasia. Inhibitory effects of Curcuma longa L. (CL) extracts on 5-alpha reductase II activity were investigated in rat prostate tissue homogenates as well as LNCaP cells expressing human 5-alpha reductase II. Hot water extract (CL-HW) of Curcuma longa L. significantly inhibited 5-alpha reductase activity by over 80% at a concentration of $100{\mu}g/mL$, whereas 20% ethanol extracts (CL-E20) of Curcuma longa L. exhibited significant inhibitory activity from $50{\mu}g/mL$. These results indicated that Curcuma longa L is a potent 5-alpha reductase II inhibitor for benign prostatic hyperplasia treatment.

• Biomolecules & Therapeutics
• /
• 제28권4호
• /
• pp.354-360
• /
• 2020

The hair cycle (anagen, catagen, and telogen) is regulated by the interaction between mesenchymal cells and epithelial cells in the hair follicles. The proliferation of dermal papilla cells (DPCs), mesenchymal-derived fibroblasts, has emerged as a target for the regulation of the hair cycle. Here, we show that vanillic acid, a phenolic acid from wheat bran, promotes the proliferation of DPCs via a PI3K/Akt/Wnt/β-catenin dependent mechanism. Vanillic acid promoted the proliferation of DPCs, accompanied by increased levels of cell-cycle proteins cyclin D1, CDK6, and Cdc2 p34. Vanillic acid also increased the levels of phospho(ser473)-Akt, phospho(ser780)-pRB, and phospho(thr37/46)-4EBP1 in a time-dependent manner. Wortmannin, an inhibitor of the PI3K/Akt pathway, attenuated the vanillic acid-mediated proliferation of DPCs. Vanillic acid-induced progression of the cell-cycle was also suppressed by wortmannin. Moreover, vanillic acid increased the levels of Wnt/β-catenin proteins, such as phospho(ser9)-glycogen synthase kinase-3β, phospho(ser552)-β-catenin, and phospho(ser675)-β-catenin. We found that vanillic acid increased the levels of cyclin D1 and Cox-2, which are target genes of β-catenin, and these changes were inhibited by wortmannin. To investigate whether vanillic acid affects the downregulation of β-catenin by dihydrotestosterone (DHT), implicated in the development of androgenetic alopecia, DPCs were stimulated with DHT in the presence and absence of vanillic acid for 24 h. Western blotting and confocal microscopy analyses showed that the decreased level of β-catenin after the incubation with DHT was reversed by vanillic acid. These results suggest that vanillic acid could stimulate anagen and alleviate hair loss by activating the PI3K/Akt and Wnt/β-catenin pathways in DPCs.

• Proceedings of the Korean Information Science Society Conference
• /
• 한국정보과학회 2004년도 가을 학술발표논문집 Vol.31 No.2 (3)
• /
• pp.574-576
• /
• 2004

현재 Peer-to-Peer (P2P) 파일 공유 시스템은 특정 데이터 항목을 저장한 노드의 위치를 어떻게 효율적으로 찾을 것인가에 대해 연구되고 있다. DHT 기반 구조는 확장 가능하며 정확한 매치(exact-match) 질의가 가능하지만, 정확한 매치가 아닌 질의에 대해서는 효율적이지 못하다. 본 논문은 DT 기반의 P2P 파일 공유 시스템에서 확장 가능한 키워드 기반 파일 검색을 제공하기 위한 메커니즘을 제안한다. 우리의 제안된 구조는 높은 능력을 가진 ' 슈퍼피어 ' 를 둠으로써 많은 파일을 가진 공통 키워드로 인친 발생하는 과도한 저장 공간 소비와 네트워크 트래픽을 감소시킨다.래픽을 감소시킨다.

• 한국전자현미경학회:학술대회논문집
• /
• 한국현미경학회 1998년도 제29차 춘계학술대회
• /
• pp.25-25
• /
• 1998

• Proceedings of the Korean Information Science Society Conference
• /
• 한국정보과학회 2004년도 가을 학술발표논문집 Vol.31 No.2 (1)
• /
• pp.46-48
• /
• 2004

Distributed Hash Table(DHT)를 기반으로 하는 structured P2P에서 복잡 질의를 통친 원하는 자료를 찾는 것은 쉽지 않다. 이와 같은 것들 중 하나가 범위 값을 이용하여 원하는 값을 찾는 range query이다. 본 논문에서는 full binary tree의 특징을 이용하여 structured P2P에서 range query를 수행할 수 있는 mapping 함수와, load balance를 지원하기 위한 방법을 제안한다 또한 실험을 통해서 이 방법들이 어느 정도의 성능을 보여주는 지도 살펴본다.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• 제35권5B호
• /
• pp.816-823
• /
• 2010

Recently research efforts for ubiquitous technology that includes RFID(Radio Frequency Deification and sensor networks are conducted very actively The architectural framework of the USN sensor network discovery service. The survey of the USN technology is conducted on four technological visions that contain USN system technology USN networking technology and USN middleware along with the service platform, With respect to each technological division domestic and worldwide leading research projects are primarily explored with their technical features and research projects are primarily explored with their technical features and research outputs. Boasted on the result of the survey we establish a USN software model that includes data sensing, sensor data storage sensor data storage sensor data naming and sensor feed name service. This main objective of this model is to provide a reference model for the facilitation of USN application developments.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권3호
• /
• pp.1033-1036
• /
• 2015

Background: Testosterone, a primary androgen in males, is converted into its most active form, dihydrotestosterone (DHT), by $5{\alpha}$-reductase type 2 (encoded by the SRD5A2 gene) in the prostate. DHT is necessary for prostatic growth and has five times higher binding affinity than testosterone for androgen receptors. We hypothesized that polymorphic variations in the SRD5A2 gene may affect the risk of benign prostatic hyperplasia and prostate cancer. Materials and Methods: We analyzed SRD5A2 gene polymorphisms in 217 BPH patients, 192 PCa cases, and 171 controls. Genotyping was undertaken using direct DNA sequencing. Genotype data were compared between cases and controls using a Chi square statistical tool. Results: We found that the A49T locus was monomorphic with 'AA' genotype in all subjects. At V89L locus, the presence of 'VV' showed a marginally significant correlation with increased BPH risk (p=0.047). At the $(TA)_n$ locus, longer TA repeats were found to be protective against BPH (p=0.003). However, neither of these polymoprhisms correlated with the risk of PCa. Conclusions: We conclude that A49T is monomorphic in the study population, VV marginally correlates with BPH risk, and longer $(TA)_n$ repeats are protective against BPH. None of these polymorphisms affect the risk of PCa.

• Korean Journal of Pharmacognosy
• /
• 제43권4호
• /
• pp.323-327
• /
• 2012

Acne, also known as Acne vulgaris, is a common disorder of human skin involving the sebaceous gland and Propionibacterium acnes (P. acnes). The purpose of this study was to demonstrate whether anti-acne herbal complex (AAHC), a functional extract from herbal complex can be used for acne treatment as a natural product. We first demonstrated anti-acne activity of AAHC in mouse acne model. Acne was induced by injecting P. acnes on the backside $2{\times}10^7$ CFUs in ICR mice and then the mice were treated with AAHC by dermal application once daily. ACFREE$^{(R)}$ (clindamicin phosphate) was used as a positive control. Treatment with AAHC decreased the P. acnes-induced skin swelling and inflammation. AAHC treatment significantly decreased serum DHT concentration in acne-induced mice. Especially, treatment of 20% AACH in mice was more effected than 40%. We next evaluated the antimicrobial property of AAHC against P. acnes, Staphylcococcus aureus (S.aureus), and Escherichia coli (E. coli). Incubation of P. acnes, S. aureus, and E. coli with AAHC showed minimal inhibitory concentration (MIC) values against the bacterial growth lower. Alamar blue method was also carried for the antibacterial activity. It was effectively MIC level at 6.25% of P. acnes. AAHC effectively inhibited the growth of S. aureus and E. coli at 0.097% on MIC level, respectively. Our results showed the potential of using AAHC as an alternative treatment for antibiotic therapy of acne and the application of AAHC as a herbal medicine for acne treatment.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• 제9권3호
• /
• pp.165-171
• /
• 2009

With rapid growth of Mobile Ad-Hoc network(MANET) and P2P service technologies, many attempts for integration of MANET and P2P service and development of such applications are actively introduced recently. The implementation of stable P2P service, however, is very difficult challenge because of the high mobility of mobile users in MANET. In this paper, we propose an efficient mobile P2P service, which shares and manages multimedia data files efficiently in mobile environment, uses distributed caches to store files considering their popularities in order to achieve high performance. The performance of proposed P2P service is evaluated by an analytic model and compared with those of existing DHT based P2P service in peer-to-peer network.

• Journal of Acupuncture Research
• /
• 제31권1호
• /
• pp.95-103
• /
• 2014

Objectives : The aim of this study is to evaluate the effect of Paljeong-san pharmac-opuncture(PJS) on the rat model of benign prostatic hyperplasia(BPH). Methods : Rats were divided into 5 groups, with 6 rats in each group. The 5 groups included sham-operated group(sham group), BPH model group(BPH group), finasteride-treated group (fina group), PJS-treated groups(PJS 10 and PJS 100 group). Testosterone was injected subcutaneously to the castrated rats except sham group for BPH model. During 4-week experimental period, finasteride(5 mg/kg) was administrated orally once daily in fina group, PJS in PJS 10(10 mg/kg) and PJS 100(100 mg/kg) group and normal saline in sham and BPH group were injected subcutaneously once daily at Jungwan($CV_{12}$). We checked prostate weights, serum concentration of dihydrotestosterone(DHT), morphologic changes of the prostate, and the amount of expression of the proliferating cell nuclear antigen(PCNA) and $5{\alpha}$-reductase gene to evaluate the effect of PJS after 4-week experimental period. Results : 1. PJS and finasteride treatment reduced prostate weights comparing with BPH group, but PJS-treated groups showed no significant changes, unlikely fina group. 2. PJS-treated groups showed significant degreases in concentration of DHT. 3. PJS-treated groups showed significant degreases concentration-dependently in the amount of expression of the PCNA and $5{\alpha}$-reductase gene. 4. PJS treatment showed shrinking of thickness in the prostatic epithelial tissue. Conclusions : PJS has the effects that improve the symptoms of BPH through inhibiting proliferation of the prostatic tissues.