• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.3
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• pp.205-216
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• 2010

Few spring sowing have been conducted on winter cereal crops for whole-crop silage use. Experiments were conducted during 2007 and 2008 at the Chungnam Agricultural Research & Extension Services. The objectives of this study were compared the spring sowing with the optimum season's sowing on growth, yield and feed quality in five winter cereal crops. The treatments consisted of 5 winter cereal crops, Youngyang (Barley, Spring habit I), Keumkang (Wheat, Spring habit II), Gogu(Rye, Spring habit estimated III), Shinyoung (Triticale, Spring habit estimated III), Samhan(Oat, Spring habit estimated II), and 3 planting dates, 18 October (optimum season's sowing), 23 February and 10 March in spring. Heading days as affected by spring sowing compared to optimum season sowing were delayed by 16~20 days in barley, wheat, rye and triticale, and 9 days in oat. The clipping dates at the optimal harvesting stage of each crop for round-baled silage in spring sowing was 8 June (yellow ripe stage) in barley, 25 May (10 days after heading) in rye, and 17 June in wheat (yellow ripe stage), triticale (milky stage) and oat (milky stage). The accumulative temperature from emergence to heading was significantly decreased as affected by spring sowing compared to optimum season's sowing, but that of sowing to emergence and that of heading to maturing was similar. The rate of spikes per tillering surveyed at each clipping date was 62.0-73.1 percent in barley, wheat, triticale and oat, and 56.0 percent in rye compared to that of optimum season sowing. The dry matter yield in spring sowing compared to 18 October was obtained about 71.7 percent in barley, 60.6 percent in wheat, 46.2 percent in rye, 70.2 percent in triticale and 110.9 percent in oat. It were increased in acid detergent fiber (ADF), neutral detergent fiber (NDF) and crude protein content, but decreased in digestible dry matter content(DDM) and relative feed value (RFV). The yield of DDM by spring sowing was decreased in barley, wheat, rye and triticale, but increased in oat. The yield of dry matter and DDM were higher in oat and triticale than that of barley, wheat and oat. So, regardless to clipping dates and cropping system, the appropriated crop for spring sowing was oat, and subsequently triticale and barley. It was not adopted for spring sowing in rye because of low rate of no. of spikes per tillers and yield. It was necessary eliminated winter growing nature by earlier sowing at the late of February after overwinter.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.3
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• pp.203-214
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• 2008

The overwintering crops, barley, wheat, oat and triticale etc, have been received in korea as high-quality roughage for round-baled silage making as livestock feed. Studies were carried out to evaluated the effects of seed blending on growth, yield and feed value between barley and wheat, triticale and oat, respectively. The results are summarized as follows : The barley was declined in the growth and spike's size as affected by sown as mixed seeding with triticale, but not effected with oat. The total fresh yield were increased by grown as mixed seeding with wheat, triticale and oat, but the dry matter yield were not significantly increased because of the low percentage of dry matter and the decrease of barley's growth. The neutral detergent fiber (NDF) and crude protein content were increased by seed blending, but acid detergent fiber (ADF) and digestible dry matter (DDM) content were decreased. The feed value was improved by seed blending of barley and wheat, but not significant at seed blending of triticale and oat. Because of the flourishing tillers, difference of heading date and abundant leaves of oat, the optimum crop for mixed seeding with barley for increment of forage productivity was oat in middle area of korea.

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.4
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• pp.311-316
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• 1998

This study was conducted to evaluate the dry matter yield and quality of mixture types with Festulolium braunii (Festuca pratensis Huds. ${\times}$ Lolium multiflorum Lam.) as a forage sources. The experiment was arranged in a randomized block design with three mixture types: (1) Festulolium braunii (seeding rate, FB 50%)+orchardgrass (OG 50%), (2) Festulolium braunii (FB 80%)+red clover(RC 20%), and (3) Festulolium braunii (FB 20%)+orchardgrass (OG 50%)+tall fescue(TF 20%)+white clover (WC 10%) and four replications. This study carried out from Sep. 1995 to May 1998 at Chungnam University. Throughout this experiment, the dry matter yields and forage quality were observed. The DM yield of FB+RC mixtures was higher than that of other mixture types, but there were no significant difference among mixture types. The FB+RC mixtures was higher than the other mixture types in the CP content(P<0.05). However, the NDF and ADF content of FB+RC mixtures were significantly lower than those of other mixture types(P<0.05). The CP and DDM yields of FB+RC mixtures were higher than those of other mixture types(P<0.05). In addition, the FB percentage of total herbage was ranged from 51% to 58% in all mixture types at the last cutting time in 1997. Based on the results mentioned above, it appears that FB has enough of possibility in mixture with other grasses, especially good mixed with RC.

• Journal of The Korean Society of Grassland and Forage Science
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• v.24 no.2
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• pp.137-144
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• 2004

The objective of this experiment was to suggest the optimum N fertilization level for turf type mixtures {Kentucky bluegrass(Newport) 40% + tall fescue(Rebell Jr.) 20%. + perennial ryegrass(Palmer II) 10% + redtop(Barricuda) 10% + red fescue(Salem) 10% + creeping bentgrass(Crenshaw) 10%}. The field trials were conducted from 1998 to 2000 at Chungnam National University in order to evaluate the dry matter yield and quality on the N levels(100, 150 and 200kg/ha). With increasing the N level, the average DM yields over 2-years of turf type mixtures significantly increased. The average total DM yields were obtained 6,551, 8,182 and 9,501kg/ha at N 100, 150 and 200kg/ha level(p<0.05), respectively. CP content and DMD were increased, whereas fibrous constituents was decreased(p<0.05), but CPDM and DDM yields were significantly increased with increasing the N levels over 2-year(p<0.05). Based on the results, considering the total DM yield and the quality of herbages which were incidentally produced from turf type mixtures, it can be suggested that the suitable N level was 200kg/ha. But increased N fertilizer usage for general purpose use and expenses on turf type mixtures should be considered in advance.

• Journal of Veterinary Clinics
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• v.22 no.4
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• pp.377-381
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• 2005

We tried to extract bone morphogenetic protein (BMP) from the freeze-dried bovine cortical bone (FBCB) for bone graft, which were defatted with chloroform-methanol for 20 days, freeze-dried at $-80^{\circ}C$ for 7 days and sterilized by ethylene oxide gas. Two kg of FBCB were pulverized in a wheel mill to $0.5-2.0mm^3$ cubic in size. The bone particles were demineralized in 0.6N HCI for 10 days at chloroform-methanol$4^{\circ}C$ and defatted with chloroform-methanol for 6 hours at room temperature, which was going to be defatting and demineralized cortical bone (DDM). For extracting BMP, DDM was agitated continuously through 72 hours with magnetic stirrer at $4^{\circ}C$ into 12 times of volume of 6 M guanidine hydrochloride (Gdn-HCl) solution containing proteinase inhibitors to protect BMP such as 2mM N-ethylaleimide, 1mM iodoacetic acid, 1mM phenylmethylsulfonyl fluoride and a sterilizer, 1mM sodium azide. The extraction procedure was repeated for three times. All extracted solution was centrifuged at 10,000 rpm for 30 min and then, the supernatant was dialyzed with 12 times of volume of deionized water at $4^{\circ}C$ for 24-72 hours, which cut off below 6,000-8,000 molecular weight. The dialyzed specimen contained crude-BMP was centrifuged, freeze-dried, and weighted. Through these processing, we could obtained $84.9\%$ as FBCB, $17.8\%$ as DDM and $0.71\%$ as crude-BMP from the wet cortical bone without cancellous bone, marrow and muscles. The crude-BMP were obtained $68.3\%$ from the first extraction, $29.6\%$ from secondary and $2.1\%$ from tertiary, respectively. It was suggested that high yield of crude-BMP migth be explained by three-time repetition of the extraction processing for crude-BMP with Gdn-Hcl sol.

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.2
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• pp.151-156
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• 1998

This study was conducted to evaluate the forage yield and quality of Fesrulolium bmunii(Festuca prafensis Huds. x Lolium multiflomm Lam.) as a forage sources. The experiment was arranged in a randomized block design with four replications from Sep. 1995 to May 1998 at Chungnam University. Treatments consisted of two forage species, Festulolium bmunii(Pau1ita) and Dactylis glomerc;d4Potomac). The dry matter yields, nutritive value, N and energy balance were observed. The yields of DM, CPDM and DDM per area(ha) were higher for Potomac (P< 0.01) than for Paulita. The content of CP showed no difference between Potomac and Paulita, but NDF, ADF and lignin contents of Paulita were absolutly lower than those of Potomac (P<0.05). The retained N and energy were also high for Paulita compare to for Potomac. It could be suggested that Paulita has higher potential for improvement of the forage quality than Potomac and it have a potential to provide as a forage sources.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.2
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• pp.101-108
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• 2007

The object of this experiment was to investigate the effect of mixture which consist of different seeding rates and species on dry matter yield and quality in Daejon area. The field trials were conducted from 2003 to 2005 at Chungnam National University in order to evaluate the dry matter yield and quality of different mixture. The experimental design includes three mixture types: Conventional mixtures, CM{orchardgrass(Potomac) 50% + tall fescue (Fawn)20% + Kentucky bluegrass(Kenblue) 20% + white clover(Regal) 10%}, red clover + mixtures, RM{orchardgrass(Potomac) 40% + tall fescue(Fawn) 20% + Kentucky bluegrass(Kenblue) 10% + red clover(Kenland) 30%) and Turf type grass + mixtures, TM {orchardgrass (Potomac) 50% + turf type grass (tall fescue, Millennium 20% + Kentucky bluegrass, Midnight 10% + perennial ryegrass, Palmer III 10%) + white clover(Regal) 10%}. The average DM yield for 2 years of red clover+mixture(11,656kg/ha) was higher than those of the other mixtures(p<0.05). The content of crude protein and dry manu digestibility were higher in red clover+mixture than in other mixtures(p<0.05). but, the content of fibrous compounds like as NDF, ADF, cellulose and lignin were lower in red clover+mixture than in other mixtures(p<0.05). The crude protein dry matter(CPDM) yield was higher in red clover + mixture(2,832kg/ha) than in conventional mixture(2,372kg/ha) and turf type + mixture(2,266kg/ha)(p<0.05). The digestible dry matter (DDM) yield was higher in red clover + mixture(8,881 kg/ha) than in conventional mixture(8,255kg/ha) and turf type + mixture(7,314kg/ha)(p<0.05). In botanical composition at last cutting time in 2005, conventional mixture were maintained orchardgrass 45%, tall fescue 22%, Kentucky bluegrass 5% and white clover 24%. Red clover + mixture were maintained orchardgrass 40%, tall fescue 22%, Kentucky bluegrass 4% and red clover 31%. turf type + mixture were orchardgrass 37%, tall fescue 23%, Kentucky bluegrass 6%, perennial ryegrass 8% and white clover 24%, respectively. As summary, DM yields and quality of mixture species and seeding rates were observed significant difference. The results of this experiment indicated that red clover + mixture was more effective in enhancing the DM yield and forage quality in Daejon area.

• Korean Journal of Plant Resources
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• v.27 no.5
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• pp.556-566
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• 2014

In this study, we collected 29 Kenaf (Hibiscus cannabinus L.) genetic resources from 10 countries to confirm the possibility of the introduction into Korea. The Kenaf (Hibiscus cannabinus L.) has the broad industrial use like feed and the 5 times more strong $CO_2$ absorption capacity compared to general plant. So recently it is considered as a good industrial source. We analyzed ecotype, growth and development, yield and feed value of collected resources. They were divided into 3 groups depending on flowering time, early maturing type blooming in early July, mid-late maturing type blooming from end of August to early September and late maturing type blooming in end of October. Early maturing type could get fruitful seed but dropped yield (average 7,895 kg/10a) and late maturing type could get high yield (average 12,572 kg/10a) but deletion. The other side mid-late maturing type could get yield over a certain level (average 11,207 kg/10a) and fruitful seed was ensured. The yields analyzed by resources indicated that K12 (Hongma 300) collected in China, K25 from India, K22 from Russia and K21 (Khonkaen 60) variety from China were more than 13,500 kg per 10a. Feed value of early maturing type had inclined to more better than other types, but like dry digestible matter (DDM) and dry matter intake (DMI) of some late maturing types like K12, K21, K22 which had a high yielding ability were more better than that of early maturing types'. So relative feed value (RFV) which considered comprehensive item for feed value were indicated K21 was 102.5, K12 was 116.2 and K22 was 120.2. 'Jangdae' the variety developed in Korea which could complement of other types' had a good quality for yield 10,975 kg/10a and 12.5% crude protein content and 101.9 of the relative feed value. So we need to be taken power to collection of mid-late maturing types resources.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.4
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• pp.317-324
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• 1999

This study was conducted to evaluate the dry matter yield and quality of mixture swards associated with Festulolium braunii(Festuca pratensis Huds. ${\times}$ Lolium multiflorum Lam.). The experiment was arranged in a randomized block design with four mixture types: A(control); orchardgrass(seeding rate 60%) + tall fescue 20% + Kentucky bluegrass 10% + white clover 10%, B; orchardgrass 60% + tall fescue 20% + Festulolium braunii 10% + white clover 10%, C; orchardgrass 60% + Festulolium braunii 20% + Kentucky bluegrass 10% + white clover 10%, and D; orchardgrass 30% + Festulolium braunii 30% + red clover 40%. This study was carried out from Sept. 1996 to Dec. 1998 at Chungnam National University. The DM yield of D mixture type was higher than that of other mixture types(P<0.05). Compared with A(control) mixture type, the DM yield of the B mixture type which substitutes Kentucky bluegrass with Festulolium braunii was higher, but the C mixture type which substitutes tall fescue with Festulolium braunii was lower than that of A(control) mixture type. The content of CP, NDF, ADF, hemi-cellulose and cellulose among mixture types shows no significant differdence. Howerever, the DMD was not significantly different among mixture types in 1997, but the DMD of C and D mixture type were significantly higher than those of other mixture types in 1998(P<0.05). On the otherhand, the yield of CP and DDM in D mixture types was higher than those of the other mixture types(P<0.05). In botanical composition, the Festulolium braunii percentage of B, C and D mixture type with mixed Festulolium braunii wasd highly maintained, especially D mixture type was ranging from 40% to 60% in experimental periods. Following the above result, It is the most favorable mixture method for Festulolium braunii to make use of top grass like orchardgrass + red clover. It is also recommened to substitute bottom grass like Kentucky bluegrass with Festulolium braunii rather than with tall fescue in A(control) mixture type.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.4
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• pp.207-214
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• 2006

The object of this experiment was to suggest the optimum utilization upon rye and hairy vetch crops in Daejeon area. The field trials were conducted from 2002 to 2004 at Chungnam National University in order to evaluate the dry matter yield and quality of rye, hairy vetch, and rye+hairy vetch mixtures. The experiment was designed with split plots (main plot : seeding rate as rye mono culture, hairy vetch monoculture, rye 80%+hairy vetch 20%, rye 60%+hairy vetch 40%, and sub plot : 4 cutting stages as boot, heading, anthesis, milk). The average DM yield for 2 years had a difference on seeding rate and harvesting stages. The DM yield of rye monoculture was higher than those of the other seeding rates. There were significant increases in advanced stages in all treatments. In chemical composition, they also had a difference on seeding rates and growth stages. Hairy vetch monoculture was higher in crude protein (CP) and dry matter digestibility (DMD), but lower in fibrous compound than those of the other seeding rates (p<0.01). The crude protein dry matter (CPDM) and digestible dry matter yield was higher in rye 80%+hairy vetch 20% mixture when it was harvested at anthesis to milk stages. The results of this experiment indicated that when it was harvested at anthesis to the milk stages, rye 80%+hairy vetch 20% mixture was more effective in enhancing the DM yield and forage quality in Daejeon area.