• Journal of Microbiology
• /
• 제34권2호
• /
• pp.206-213
• /
• 1996

NADH-quinone reductase was purified 22-fold from the cytosolic fraction of Streptomyces sp. Imsnu-1 to apparent hemogenity, with an overall yield of 9%, by the purification procedure consisting of ammonium, sulfate precipitation and DEAE Sephacryl S-200 and DEAE 5 PW chromatographies. Thes molecular mass of the enzyme determined by gel filtration chromatography was found to be 110 kDa. SDS-PAGE revealed that the enzyme consists of two sugunits with a molecular mass of 54 kDa. The enzyme contained 1 mol of FMN per subunit as a cofactor. The $A_{272}$ A$_{457}$ ratio was 6.14 and the molar extinction coefficients were calculated to be 20, 800 and 25, 400M$^{-1}$ $cm^{-1}$ / AT 349 AND 457 nm, respectively. The N-terminal sequence of the enzyme contained the highly conserved fingerprint of ADP-binding domain. The enzyme used NADH as an electron donor and various quinones as electron acceptors. Cytochrome c was practically inactive. Air-stable flavin semiquinone was produced by the addition of NADH to the enzyme. Also, naphthosemiquinone was detected in the reaction mixture containing the enzyme.

• 한국환경농학회지
• /
• 제22권1호
• /
• pp.16-25
• /
• 2003

농촌지역의 도시화와 산업화에 따라 농촌 수질환경의 오염이 심화되고 있다. 경북 안동의 중산간지대에 위치한 만운저수지는 유역면적 $23.8\;km^2$, 수표면적 $0.4\;km^2$, 저수량 $2.0{\times}10^6\;m^3$의 부영양화 된 중규모 농업용저수지로서 1999년 수질환경특성조사를 실시하여 오염된 유사 농업용 저수지의 수질특성이해에 중요한 자료로 활용하고자 하였다. 만운저수지의 수질에 영향을 미치는 유역면적값(DA)/호수면적값(SA)의 비가 56.1로 국내 다른 농업용 저수지보다 높게 나타나 유역의 오염물질 부하가 높은 편이며, 평균 수심과 관련된 유효저수량(ST, $m^3$)/수표면적(SA, $m^2$)비는 4.79로서 안동호, 팔당호보다 낮고 삽교담수호 등과 비슷한 값을 보여주어 부영양화의 잠재성이 높은 것으로 나타났다. 만운저수지의 수질조사결과 COD는 11.1 mg/L, 총질소 1.426 mg/L로 환경기준 중 생활환경 IV등급인 농업용수 수질기준을 초과하였으며, 총인은 0.093 mg/L로 IV등급의 수질을 나타내고 있었다. 유입하천은 총질소($1.426{\sim}3.809\;mg/L$)가 비교적 높게 나타나고 있어 총질소에 기준한 수질개선 방안이 요구되었다. 식물성플랑크톤 조사결과 Oscillatoria와 근연종인 Lyngbya가 우점하였으며, 동물성플랑크톤은 대부분의 지역에서 적조현상을 일으키는 와편모충인 Gymnodinium spp., Peridinium spp., 암색편모충인 Cryptomonas spp.이 우점하였다. 부영양화도지수(TSI)를 이용한 1999년도의 시기별 영양상태 변화는 9월에 과부영양상태를 보였으며, 연도별 $TSI_M$지수는 '96년, '97년에 부영양, '98년의 과영양으로 영양상태가 증가하였으나, 강우가 다소 많았던 '99년에는 강우의 영향으로 다소 개선되어 나타났다. 결론적으로, 저수지의 물리구조적 유역특성 및 수리학적 특성상 만운저수지는 부영양화에 취약한 특성을 가지고 있으며, 강우량에 영향을 받고는 있으나 전반적으로 부영양화도가 증가하는 추세를 보인다. 따라서, 이와 같은 추세변화를 정확히 인식하고, 그에 적절한 대책을 마련하기 위해 농업용수 수질측정망 운영이 상당히 중요하게 인식되어지며, 수질환경 특성을 감안한 합리적 운영이 이루어져야 할 것으로 사료된다.

• Plant Breeding and Biotechnology
• /
• 제7권3호
• /
• pp.186-199
• /
• 2019

We selected 68 Chinese maize inbred lines to understand the genetic diversity, population structure, and marker-trait associations for eight agronomic traits and 50 simple sequence repeats (SSRs) markers. In this study, effective traits, such as days of anthesis (DA), days of silking (DS), ear height (EH), plant to ear height ratio (ER), plant height (PH), and leaf width (LW) were divided into PC1 and PC2 by PCA analysis for maize inbred lines. Genetic diversity analysis revealed a total of 506 alleles at 50 SSR loci. The mean number of alleles per locus was 10.12. The averages of genetic diversity (GD) and polymorphic information content (PIC) values were 0.771 and 0.743, respectively. Based on a membership probability threshold of 0.80, the population structure revealed that the total inbred lines were divided into three major groups with one admixed group. A marker-trait association using Q + K MLM showed that nine SSR markers (bnlg1017, umc2041, umc2400, bnlg105, umc1229, umc1250, umc1066, umc2092, and umc1426) were related with seven agronomic traits. Among these SSR markers, eight SSR markers were associated with only one agronomic trait (DA, DS, ER, LL, LW, PH, and ST), whereas one SSR marker (umc1229) was associated with two agronomic traits (DA and ST). These results will help in optimizing the choice of inbred lines for cross combinations, as well as in selecting markers for further maize breeding programs.

• 보존과학연구
• /
• 통권20호
• /
• pp.121-137
• /
• 1999

Among pigment used at work of Dan-Chung, Emerald Green is specific illuminating fluorescent light of green. It is very difficult to change other organic or inorganic pigment. All of the internal high class pigment has rare light. But Emerald Green is superior to fresh color and stability out of industrial chemical products. It forms over 50% of quantity and importance of a pattern painting. Emerald Green prohibited to produce because of its toxicpollutants, so required to changing pigment development. It is characterized to excellent color, convenient work, economical, against-sunlight, against-air pollutant and durability. The result of a test is follows; 1. We are investigated into producing internal natural Emerald Green, import external pigment and industrial synthesis method etc. but unable to buy because of its toxic pollutant. 2. We are made six samples by yellowish and green is hpigment mixing. We tested on against sunlight and air pollutant. The best mixing ratio is follows. Titanium Dioxide R760 : 18g- Chalk, White Wash : 10g- Permanent Yellow : 7g- Cyanine Green : 8g- Chrome Yellow : 3g- Resin(Vehicle) : Acryl Emulsion(Styrene + 2-Ethyl HexylAcrylate + Methyl Meth Acrylate) 8%

• 한국환경보건학회지
• /
• 제49권6호
• /
• pp.324-333
• /
• 2023

Background: Human exposure to phthalates in indoor environments occurs via dermal absorption, oral ingestion of indoor dust, and inhalation of indoor air. However, systematic studies to investigate the exposure rate to phthalates among the three exposure routes in indoor environments are currently limited. Objectives: A theoretical exposure ratio between inhalation and oral exposure was calculated based on the total amount of di(2-ethyl-hexyl) phthalate (DEHP) emitted into indoor air and deposited into floor dust in a test house. Methods: Flooring and wallpaper containing DEHP were installed in a test house and the concentration of DEHP in both indoor air and floor dust were monitored for five months. Based on the measured DEHP concentrations, the theoretical total amount ratio of DEHP that could be exposed through inhalation and oral ingestion was calculated. Results: Considering the period of operation in the test house, the theoretical total amount of DEHP through inhalation and oral ingestion exposures in the entire test house space was calculated to be 0.014 mg and 5.5 mg, respectively. The exposure ratio of the two routes between inhalation and oral exposure corresponding to the total DEHP amount in flooring and wallpaper was 6.0×10^-7% and 2.3×10^-4%, indicating that theoretical oral exposure to DEHP is approximately 380 times higher than inhalation. Conclusions: Monitoring results from a test house has shown that oral exposure is the main exposure route for DEHP in indoor environments. The experimental design employed in this study and theoretical exposure ratio obtained can be applied to investigate actual exposure to DEHP and to determine the exposure characteristics of various types of semi-volatile organic compounds.

• 지역사회간호학회지
• /
• 제30권3호
• /
• pp.295-306
• /
• 2019

Purpose: There has been a growing recognition that person-centered care enhances the quality of life of nursing home residents with dementia. This study was conducted to develop a person-centered dementia care online education program for direct care staff in long-term care facilities. Methods: Delphi method with expert group was used to validate contents. We developed 61 draft items based on literature review. Twenty experts participated in consecutive three round surveys including 5-point Likert scale questions and open-ended questions. Based on experts' opinions, the content validity ratio for content validity and the coefficient of variation for stability were calculated. Results: Three-round Delphi surveys and additional feedback from the expert panel established a consensus of core contents: 1) dementia (7 categories), 2) person-centered care (6 categories), 3) communication (8 categories), and 4) behavioral and psychological symptoms of dementia (6 categories). Specific sub-categories in each category were differentiated according to the job qualifications (65 sub-categories for registered nurses, 64 sub-categories for nursing aids, and 41 sub-categories for personal care workers). Conclusion: This delphi study identified person-centered dementia education curricula, in which the person-centered approach should be a key policy priority in Korean long-term care system. Now it is urgently needed to develop education programs utilizing online platforms that enable efficient and continuous learning for long-term care staff, which can contribute to behavior changes in the person-centered dementia care approach and improvement of care quality in long-term care facilities.