• Explosives and Blasting
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• v.32 no.2
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• pp.25-30
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• 2014

Although the construction noise and vibration are transient and intermittent, their impact on the surrounding environment is huge. Since the construction equipment noise and vibration is usually transmitted because of the long distance, the sound insulation and the proper design of anti-vibration measures are very difficult. The regulation requires that the noise and vibration caused by the construction equipments should be measured within 30m from the source, whereas the blasting noise and vibration should be measured at least 60m and 160m away from the source, respectively. Instead of the 2D modelling mainly conducted so far, the 3D analysis of noise and vibration with the consideration of the height and size of the building, mountains and hills in the vicinity of the source is necessary.

• Journal of Advanced Navigation Technology
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• v.14 no.2
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• pp.170-175
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• 2010

This article proposed polarization selective antenna for UHF RFID system. The proposed antenna is consist of microstrip patch antenna with dual feeding and two SPDT switches and a SP4T switch and 3dB hybrid coupler. Through control of voltage of switches, the proposed reader antenna can select horizontally linear polarization, vertically linear polarization, left-hand circular polarization (LHCP) and right hand circular polarization (RHCP). The proposed reader antenna satisfied 2:1 VSWR at 902MHz ~ 928MHz. and 3dB under AR(axial ratio). And it can select appropriative polarization with user environment and tag polarization. So it minimize PLF and increased reading distance.

• Journal of Korean Physical Therapy Science
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• v.11 no.1
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• pp.20-27
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• 2004

It is widely accepted that smooth muscle contraction is triggered by intracellular $Ca^{2+}$ ($[Ca^{2+}]_i$) released from intracellular $Ca^{2+}$ stores such as sarcoplasmic reticulum (SR) and from the extracellular space, The increased $[Ca^{2+}]_i$ can phosphorylate the 20-kDa myosin light chain ($MLC_{20}$) by activating MLC kinase (MLCK), and this initiates smooth muscle contraction. In addition to the $[Ca^{2+}]_i$-MLCK-tension pathway, a number of intracellular signal molecules, including mitogen-activated protein kinase (MAPK), protein kinase C (PKC), phosphatidylinositol 3-kinase (PI3K), and Rho-associated coiled coil-forming protein kinase (ROCK), play important roles in the regulation of smooth muscle contraction. However, the mechanisms regulating contraction of caldesmon (CaD), actin-binding protein, are not entirely elucidated in the presence of $Ca^{2+}$. It is known that CaD tightly interacts with actin and inhibits actomyosin ATPase activity. Therefore, the purpose of the present study was to investigate the roles of $Ca^{2+}$-dependent CaD in smooth muscle contraction. Endothelin-1 (ET-1), G-protein coupled receptor agonist and vasoconstrictor, increased both vascular smooth contraction and phosphorylation of CaD in the presence of $Ca^{2+}$. These results suggest that ET-1 induces contraction and phosphorylation of CaD in rat aortic smooth muscle, which may he mediated by the increase of $[Ca^{2+}]_i$.

• BMB Reports
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• v.47 no.2
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• pp.115-120
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• 2014

In this study, we show that Mycobacterium avium subsp. paratuberculosis MAP1305 induces the maturation of bone marrow-derived dendritic cells (BMDCs), a representative antigen presenting cell (APC). MAP1305 protein induces DC maturation and the production of pro-inflammatory cytokines (Interleukin (IL)-6), tumor necrosis factor (TNF)-${\alpha}$, and IL-$1{\beta}$) through Toll like receptor-4 (TLR-4) signaling by directly binding with TLR4. MAP1305 activates the phosphorylation of MAPKs, such as ERK, p38MAPK, and JNK, which is essential for DC maturation. Furthermore, MAP1305-treated DCs transform naive T cells to polarized $CD4^+$ and $CD8^+$ T cells, thus indicating a key role for this protein in the Th1 polarization of the resulting immune response. Taken together, M. avium subsp. paratuberculosis MAP1305 is important for the regulation of innate immune response through DC-mediated proliferation of $CD4^+$ and $CD8^+$ T cells.

• Journal of Korean Society for Atmospheric Environment
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• v.16 no.5
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• pp.529-537
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• 2000

Several Pt-based oxidation catalysts with different loading were prepared with various metal precursor solutions and characterized with H$_2$ chemisorption and TEM for Pt particle size. V was added to Pt-based catalyst for inhibiting SO$_2$oxidation reaction, as result, Pt-V/Ti-Si catalyst prepared by ERMS(Free Reduced Metal in Solution) method showed high enough activity and better inhibition on SO$_2$oxidation than Pt only catalyst. Optimum Pt particle size for diesel oxidation reaction turned out to be the size of around 20 nm. A prototype catalyst was prepared for light=duty diesel passenger car, and teated for the emission reduction performance with Korean regulation test mode(CVS-75 mode) on chassis dynamometer. The catalyst shows the performance reduction of 75~94% for CO, 53~67% for HC and 10~31% for PM. In the case of heavy-duty diesel catalyst, the domestic formal regulation teat mode D-13 was adopted for both Na engine and Turbo engine. The conversions of CO and THC are high enough(86% and 41%) while the reductions of NOx and PM are relatively low(3~11%).

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.6
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• pp.788-795
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• 2015

Two dimensional-fluorescence difference gel electrophoresis (2D DIGE) is an emerging technique for comparative proteomics, which improves the reproducibility and reliability of differential protein expression analysis between samples. The purpose of this study was to investigate bovine pregnancy-specific proteins in the proteome between bovine pregnant and non-pregnant serum using DIGE technique. Serums of 2 pregnant Holstein dairy cattle at day 21 after artificial insemination and those of 2 non-pregnant were used in this study. The pre-electrophoretic labeling of pregnant and non-pregnant serum proteins were mixed with Cy3 and Cy5 fluorescent dyes, respectively, and an internal standard was labeled with Cy2. Labeled proteins with Cy2, Cy3, and Cy5 were separated together in a single gel, and then were detected by fluorescence image analyzer. The 2D DIGE method using fluorescence CyDye DIGE flour had higher sensitivity than conventional 2D gel electrophoresis, and showed reproducible results. Approximately 1,500 protein spots were detected by 2D DIGE. Several proteins showed a more than 1.5-fold up and down regulation between non-pregnant and pregnant serum proteins. The differentially expressed proteins were identified by MALDI-TOF mass spectrometer. A total 16 protein spots were detected to regulate differentially in the pregnant serum, among which 7 spots were up-regulated proteins such as conglutinin precursor, modified bovine fibrinogen and IgG1, and 6 spots were down-regulated proteins such as hemoglobin, complement component 3, bovine fibrinogen and IgG2a three spots were not identified. The identified proteins demonstrate that early pregnant bovine serum may have several pregnancy-specific proteins, and these could be a valuable information for the development of pregnancy-diagnostic markers in early pregnancy bovine serum.

• Special Issue of the Society of Naval Architects of Korea
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• 2017.10a
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• pp.19-27
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• 2017

In spite of adoption of Intact Stability Code Part A as mandatory, the accidents have been occurred frequently caused by dynamic stability. Therefore International Maritime Organization (IMO) is revising the Intact Stability Code to prevent the cause of accidents. The result of 4th meeting of Sub-committee on Ship Design and Construction (SDC), five mode of the cause was defined and establishing each draft regulations. We consider physical background, mathematical description, draft regulations for five failure mode and sample calculations for ships built by STX Offshore & Shipbuilding.

• Journal of Industrial Technology
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• v.26 no.A
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• pp.3-15
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• 2006

To determine the optimum ventilation systems during long tunnel excavation, the velocity vector profile and the contaminant's distribution at working place are studied using 2-D, 3-D numerical analysis. The main results can be summarized as follow; In case of long tunnels, blower-exhaust-mixture types which enable to use soft blast ducts is most appropriate in terms of ventilation and economical efficiency. Of the same ventilation types, ventilation efficiency has a difference according to blast ducts and the distance between fan and working place. The 3-D numerical result shows that arranging blower and exhaust ducts in the right and left corners of the tunnel respectively is effective to discharge contaminant. The result of the real measurement shows that CO concentration can be reduced to below 50 ppm, which is regulation value, as 16-minutes fan operation goes on.

• Transactions of the Korean Society of Automotive Engineers
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• v.9 no.2
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• pp.99-106
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• 2001

NHTSA has been conducting biomechanical studies to reduce inujuries sustained sustained during automotive collision. Furthermore, NHTSA added the regulation to the FMVSS 201, limiting the equivalent HIC(Head Injury Criterion) value under 1000. In the presont work, a methodology was developed for the optimum design of the A-pillar trim with rib-structures. The design variables for the rib-strucrures were the transverse spacing, the longitudinal spacing, and the thickness. The required sets of the design varibles were decided based on the design of experiments. The head impact simulations were carried out using the LS-DYNA3D, and the HIC(d) values were computed using the resulrs of the head impact simulation. The objective function was constructed using the response surface methed (RSM). When the obtained optimum values were not inside the region of interest, the design proceduers were repeated by changing the region of interest. Finally, an A-pillar trim with rib-structures, which resulred in HIC(d) value under 850 for 15 mph head-trim impact, was developed.

• Development and Reproduction
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• v.15 no.1
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• pp.25-30
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• 2011

Embryonic stem (ES) cells can self-renew maintaining the undifferentiated state. Self renewal requires many factors such as Oct4, Sox2, FoxD3, and Nanog. NF-${\kappa}B$ is a transcription factor involved in many biological activities. Expression and activity of NF-${\kappa}B$ increase upon differentiation of ES cells. Reportedly, Nanog protein directly binds to NF-${\kappa}B$ protein and inhibits its activity in ES cells. Here, we found a potential binding site of NF-${\kappa}B$ in the human Nanog promoter and postulated that NF-${\kappa}B$ protein may regulate expression of the Nanog gene. We used human embryonic carcinoma (EC) cells as a model system of ES cells and confirmed decrease of Nanog and increase of NF-${\kappa}B$ upon differentiation induced by retinoic acid. Although deletion analysis on the DNA fragment including NF-${\kappa}B$ binding site suggested involvement of NF-${\kappa}B$ in the negative regulation of the promoter, site-directed mutation of NF-${\kappa}B$ binding site had no effect on the Nanog promoter activity. Furthermore, no direct association of NF-${\kappa}B$ with the Nanog promoter was detected during differentiation. Therefore, we conclude that NF-${\kappa}B$ protein may not be involved in transcriptional regulation of Nanog gene expression in EC cells and possibly in ES cells.