• Title/Summary/Keyword: D10

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Electrostatic Immobilization of D-Xylose Isomerase to a Cation Exchanger for the Conversion of D-Xylose to D-Xylulose (D-xylose에서 D-xylulose로의 전환을 위한 D-xylose Isomerase의 정전기적 고정화)

  • Hang, Nguyen Thi;Kim, Sung-Gun;Kweon, Dae-Hyuk
    • Microbiology and Biotechnology Letters
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    • v.40 no.2
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    • pp.163-167
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    • 2012
  • Since D-xylose is not fermentable in Saccharomyces cerevisiae, its conversion to D-xylulose is required for its application in biotechnological industries using S. cerevisiae. In order to convert D-xylose to D-xylulose by way of an enzyme immobilized system, D-xylose isomerase (XI) of Escherichia coli was fused with 10-arginine tag (R10) at its C-terminus for the simple purification and immobilization process using a cation exchanger. The fusion protein XIR10 was overexpressed in recombinant E. coli and purified to a high purity by a single step of cation exchange chromatography. The purified XIR10 was immobilized to a cation exchanger via the electrostatic interaction with the C-terminal 10-arginine tag. Both the free and immobilized XIR10 exhibited similar XI activities at various pH values and temperatures, indicating that the immobilization to the cation exchanger has a small effect on the enzymatic function of XIR10. Under optimized conditions for the immobilized XIR10, D-xylose was isomerized to D-xylulose with a conversion yield of 25%. Therefore, the results of this study clearly demonstrate that the electrostatic immobilization of XIR10 via the interaction between the 10-arginine tag and a cation exchanger is an applicable form of the conversion of D-xylose to D-xylulose.

Effects of Stocking Density on Growth and Survival of Sea Cucumber, Apostichopus japonicus in the Indoor Multiple Tank (육상 다단식 사육시스템을 이용한 어린 돌기해삼(Apostichopus japonicus)의 수용밀도에 따른 성장과 생존)

  • KIM, Tae-Ik;SON, Maeng-Hyun;CHO, Jae-Kwon;GO, Gyeong-Dong;JIN, Young-Guk
    • Journal of Fisheries and Marine Sciences Education
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    • v.28 no.5
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    • pp.1407-1416
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    • 2016
  • This study was conducted to investigated the effects of stocking density on growth and survival of sea cucumber, Apostichopus japonicus in the indoor multiple tank for 12 weeks. There were six treatments(stocking densities) in this experiment, i.e. 1, 2, 3, 4, 5 and 10 individuals per 10 L plastic aquarium($W41{\times}H24{\times}D11cm$, represented as D1, D2, D3, D4, D5 and D10, respectively). Each treatment had ten replicates. The results showed growth variation of sea cucumber, Apostichopus japonicus decreased with the increase of stocking densities. After the end of the experiment, no significant differences(P>0.05) between D1~D5 treatment group whereas significant differences(P<0.05) between D1 and D10 treatment group. Survival rate showed D1~D10 were 90%, 90%, 100%, 95%, 94% and 72%, respectively. Especially, significant differences(P>0.05) between D1~D5 and D10 treatment group. The results indicated optimum of stocking density for sea cucumber, Apostichopus japonicus within five individuals per 10 L plastic aquarium($0.05individuals/m^2$).

Analysis of Jamming Interference Characteristics in Nonlinear DRT Satellite Transponder System (비선형 DRT 위성 중계시스템의 재밍 간섭 특성 분석)

  • 이동형;유흥균;김기근;최영균
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.25 no.8B
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    • pp.1341-1347
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    • 2000
  • For the DRT satellite transponder system, BER and total SNR to be required in the earth terminal are evaluated under the condition of HPA nonlinearity in the FBJ(full-band jamming) or PBJ(partial-band jamming) of uplink and downlink. In case that the satellite Inter bandwidth( Ws) is same to the earth terminal bandwidth($W_r$),in conditions of uplink JSR 10[dB], downlink JSR 10[dB] and processing gain 30[dB], linear transponder system shows that uplink SNR needs to be 14.2[dB] to achieve the total SNR 10[dB] requirement in downlink SNR 14[dB]. However, Nonlinear transponder system with OBO(output backoff) 2[dB] requires 20.1 [dB] uplink SNR. From the above results, the nonlinearity of HPA in the satellite transponder causes the degradation of BER performance so that it is of interest to consider the power increase.

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Phylogeny of Subgenus Drosophila (Drosophilidae: Drosophila) in Korea by Allozyme and Soluble Protein Analysis (동위효소와 수용성단백질 분석에 의한 한국 초파리아속 10종의 계통)

  • Eun Young Joo;Nam Woo Kim
    • Animal Systematics, Evolution and Diversity
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    • v.19 no.2
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    • pp.217-225
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    • 2003
  • This study was conducted to ascertain interspecific relationships by analyzing allozyme and soluble proteins of ten species in the Drosophila (Drosophila) to form a part of systematic studies of Korean drosophilids. The results of allozyme and TDE analysis showed that D. (D.) curvispina and D (D.) tsigana had the furthest genetic distance. On the other hand, the genetic distance between D (D.) angularis and D (D.) brachynephros was extremely close. And, ten species of the subgenus Drosophila can be divided into the first group of D. (D.) virilis, D. (D.) tsigana and D. (D.) lacertosa , and the second group consisted of four subgroups; the first subgroup clustered D. (D.) angularis and D (D.) brachynephros, the second subgroup clustered D. (D.) unispina and D. (D.) curvispina, the third subgroup of D (D.) takadai and D. (D.) kuntzei and the fourth subgroup of D. (D.) nigromaculata alone.

Analysis of Vertex Refractive Power Accuracy of Soft Contact Lens with Holder Type (홀더종류에 따른 소프트 콘택트렌즈의 정점굴절력 측정의 정확도 분석)

  • Lee, Min-Jae;Sung, A-Young
    • Journal of Korean Ophthalmic Optics Society
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    • v.20 no.2
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    • pp.105-115
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    • 2015
  • Purpose: The accuracy for measuring the refractive power of hydrogel contact lenses by spectacle lens holder and contact lens holder was evaluated. The accuracy for each sample was also analyzed with water content and diopter categories. Methods: The hydrogel contact lenses used for measurement were classified into three categories in water content (38%, 43%, 58%). Also, three diopter categories of refractive power were used such as -3.000 D, -7.000 D, -10.000 D. And also, the reliability of measurement results were evaluated by measuring refractive power with spectacle lens holder and contact lens holder using an Manual lensmeter. Results: In case of spectacle lens holder method, the average value of refractive power was -3.3273D for -3.0000 D, -7.1306 D for -7.0000 D and -10.2944 D for -10.0000 D, respectively. In case of contact lens holder method, the average value of refractive power was -3.1060 D for -3.0000 D, -7.0028 D for -7.0000 D and -10.2611 D for -10.0000 D, respectively. In measurement of all diopters, the accuracy of contact lens holder method was better than spectacle lens holder method. Conclusions: From these results, it is judged that the refractive power of soft contact lens by manual lensmeter with contact lens holder have a higher accuracy than spectacle lens holder.

Wide Bandwidth Circularly Polarized Aperture Coupled Microstrip Antenna using Cross-slot (십자 슬롯을 이용한 광대역 원형편파 적층 개구결합 마이크로스트립 안테나)

  • 양태식;이범선
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.11 no.5
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    • pp.748-754
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    • 2000
  • A novel single feed wide band CP stacked microstrip antenna using crossed slots has been designed, fabricated and measured. For the single rediating element the designed 10dB return loss bandwidth is 34.5%99.45~13.54 GHz), 3dB axial ratio bandwidth is 18.7%(11.17~13.39GHz), and 6 dB gain bandwidth is 29%(10.21~13.64GHz). For the 2$\times$2 array designed using a sequential rotation method, the 10dB return loss bandwidth is 35.9%(9.69~13.94GHz), 3dB axial ratio bandwidth is 34.6GHz (9.93~14.03GHz), and 6dB gain bandwidth is 27.4%(10.35~13.6GHz). For the fabricated 8$\times$8 array antenna, the 10dB return loss bandwidth is 27.3%(10.17~13.41GHz), 3dB axial ratio bandwidth is 27.9GHz(10.1~13.4GHz), and the radiation pattern is good agreement with theory. This antenna can be used for broadband applications for communications or broadcasting in Ku band.

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Effect of Water Management after Fertilizer Application on Fate and Efficiency of Applied Nitrogen (시식 후 물관리 방법이 실소의 동태 및 이용효율에 미치는 영향)

  • 이변우;명을재;최관호
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.2
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    • pp.157-166
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    • 1995
  • The fate and use efficiency of applied nitrogen were evaluated in a pot experiment with different fertilizers and water management practices during 30days after fertilizer application. N-P-K compound fertilizers, 13-10-1l(F-l) for upland Crop use and 15-10-10(F-3) for rice Crop use, and mixed fertilizer, 21-17-17(F-2) for basal dressing in rice were used. Fertilizers corresponding to 1.8g N were mixed thoroughly with the whole volume of sandy loam soil in a pot. The pots were flooded upto 3cm above soil surface for O(0dF), 10(10dF), 20(20dF), and 30(30dF) days after fertilizer application and all the treatments were flooded continuously from 30 days after fertilizer application. During the flooding period water percolation rate was adjusted to 2.5mm/day. Rice seedlings were transplanted 40 days after fertilizer application. The pH of infiltrated water increased with increasing duration of flooding. The pH of F-2 was higher than those of F-1 and F-3 between which there were no differences. The applied nitrogen remained 23% in F-1, 29% in F-2, and 29.1 % in F-3, and 45.0% in 0dF, 26.6% in 10dF, 24.8% in 20dF, and 20.3% in 30dF as inorganic nitrogen at 63 days after fertilizer application. Nitrogen losses by leaching amounted to 51.3%, 32.1% and 48.1% of applied nitrogen in F-1, F-2 and F-3, respectively. Nitrogen leaching losses increased with increasing duration of flood- ing, amounting to 25.7%, 29.8%, 32.7%, and 35.8% in 0dF, 10dF, 20dF and 30dF, respectively. Gaseous loss of applied nitrogen was greatest in F-2, followed by F-1 and F-3. Total loss of nitrogen due to gaseous volatilization and leaching was greatest in F -1, followed by F -2 and F-3, and were greater in the treatments with longer flooding after fertilizer application. Nitrogen recovery by rice shoot until 72 days after transplanting were 23.2%, 24.7% and 27.4% of applied nitrogen in F-1, F-2 and F-3, respectively and 34.1%, 25.5%, 21.1%, and 21.2% in 0dF, 10dF, 20dF and 30dF, respectively.

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On the Phase Variation and Implementation of If Module for WLL CDMA System (WLL용 CDMA 시스템 IF 모듈의 구현 및 위상 특성)

  • 강병권;김선형
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.4 no.1
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    • pp.219-226
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    • 2000
  • In this paper, we design and implement a IF(intermediate frequency) module for WLL(wireless local loop) CDMA(code division multiple access) basestation. The implemented IF transceiver is consists of transmitter, receiver and local oscillator. The considered signal bandwidth is 10 MHz and the local carrier frequency is 40 MHz. As test results, the If transmitter output power is -5dBm $\pm3dB$when the baseband input is -10dBm $\pm3dB$, and the IF receiver output power is -10dBm $\pm3dB$when the IF input is -5dBm $\pm3dB$. Also the AGC(automatic gain control) circuit has dynamic range of 9 dB from -7dBm to +2dBm with output power 2dBm. And the group delay characteristic is analyzed by comparing the phase delay from 1 MHz to 5 MHz and the phase distortion is very low. We can conclude that this IF system can be applied to high speed data rate communication system.

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Systematic Relationships Among Ten Species of Subgenus Drosophila (Diptera: Drosophilidae) in Korea (한국 초파리아속(쌍시목: 초파리과) 10종에 대한 계통적 유연관계)

  • Nam Woo Kim;Eun Young Joo
    • Animal Systematics, Evolution and Diversity
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    • v.19 no.2
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    • pp.167-176
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    • 2003
  • This study sought to observe systematic relationships through taximetrical analyses by morphological characters among the 10 species, on three species in the virilis section (D. virilis, D. tsigana and D. lacertosa) and seven species in the quinaria section (D. angularis, D. brachvnephros, D. curvispina, D. kuntzei, D. nigromaculata, D. takadai and D. unispina) of the subgenus Drosophila. In the cluster and the cladistic analysis among the members of subgenus Drosophila, 10 species was divided into the 1st group of D. virilis, D. tsigana, and D. lacertosa and the 2nd group of D. angularis, D. brachynephros, D. curvispina, D. kuntzei, D. nigromaculata, D. takadai, and D. unispina. In cluster analysis, the 2nd group had three sister groups; one sister group that clustered D. angularis and D. brachynephros then D. unispina was clustered to them, another sister group clustered D, curvispina and D. takadai then D. kuntzei was clustered to them and the other sister group of D. nigromaculata. In the 10 species, D. virilis and D. lacertosa were the first to be divided and then D. tsigana. Although 1st group which D. virilis was belonged can be determined as more primitive than the 2nd group, it seemed that this group was not the direct ancestor of the 2nd group, rather there should be another ancestor. Among the quinaria species group, D. nigromaculata was the first to be divided and D. kuntzei was the most recent species to be divided.

Resource Allocation Scheme for D2D Communications in Multi-Cell Environments (다중 셀 환경에서 단말 간 직접 통신을 위한 자원 할당 방식)

  • Oh, Sung-Min;Lee, Changhee;Yun, Miyoung;Shin, Jaesheung;Park, Ae-Soon
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.39A no.10
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    • pp.601-609
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    • 2014
  • This paper proposes a resource allocation scheme suitable for D2D communications in multi-cell environment. In order to solve the inter-cell interference, the proposed scheme allocates the pre-assigned resource group and shares the information with neighbor cells. This paper also proposes a power control scheme for D2D communication to enhance the cell throughput. By the simulation results, the average SINR of the cellular uplink and D2D communication link are mostly higher than 10 dB when the proposed scheme is applied. On the other hand, with the inter-cell non-coordinated resource allocation scheme, the average SINR of the D2D communication link are decreased by 0 dB. In addition, the proposed scheme can enhance the cell throughput up to 8 % compared with the inter-cell non-coordinated resource allocation scheme.