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Isolation and Characterization of Starch-hydrolyzing Pseudoalteromonas sp. A-3 from the Coastal Sea Water of Daecheon, Republic of Korea (대한민국 대천 해안에서 분리한 전분 분해능을 갖는 Pseudoalteromonas sp. A-3 균주의 특징 및 동정)

  • Chi, Won-Jae;Park, Da-Yeon;Jeong, Sung-Cheol;Chang, Yong-Keun;Hong, Soon-Kwang
    • Microbiology and Biotechnology Letters
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    • v.39 no.4
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    • pp.317-323
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    • 2011
  • Strain A-3, an amylase-producing bacteria, was isolated from coastal seawater near Daecheon in the Republic of Korea. It was seen to possess a single polar flagella and grow well, on ASW-YP agar plates, at temperatures of between $20-37^{\circ}C$. However, it grew more slowly at the temperatures of $15^{\circ}C$ and $40^{\circ}C$. Similarly, it was observed to grow abundantly, in an Artificial Sea Water-Yeast extract-Peptone (ASW-YP) liquid medium, in a pH range of 6-9, but not grow at pHs of 4-5 and a pH of 10. Strain A-3 was noted as being close to Pseudoalteromonas phenolica O-$BC30^T$, Pseudoalteromonas luteoviolacea $NCIMB1893^T$, Pseudoalteromonas rubra $ATCC29570^T$, and Pseudoalteromonas byunsanensis $FR1199^T$, with 98.30%, 97.86%, 97.78%, and 97.25% similarities respectively, in its 16S rRNA sequence. A phylogenetic tree revealed that strain A-3 and P. phenolica O-$BC30^T$ belong to a clade. However, strain A-3 differed from P. phenolica O-$BC30^T$ in relation to a number of physiological characteristics. Strain A-3 exhibited no growth above 5% NaCl concentrations, no utilization of D-glucose, D-mannose, D-maltose, or D-melibose, and no lipase (C-14) activity. All of these properties strongly indicate that strain A-3 is distant from P. phenolica O-$BC30^T$ and thus led us to name it Pseudoalteromonas sp. A-3. Pseudoalteromonas sp. A-3 produces ${\alpha}$-amylase throughout growth. Maximal amylase activities of 144.48 U/mL and 149.20 U/mL were seen at pH 7.0 and $37^{\circ}C$, respectively. Pseudoalteromonas sp. A-3's high, stable production of ${\alpha}$-amylase in addition to its biochemical features, such as alkalitolerance, suggest that it is a good candidate for industrial applications.

Effect of Dietary Copper Sources (Cupric Sulfate and Cupric Methionate) and Concentrations on Performance and Fecal Characteristics in Growing Pigs

  • Huang, Y.;Zhou, T.X.;Lee, J.H.;Jang, H.D.;Park, J.C.;Kim, I.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.23 no.6
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    • pp.757-761
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    • 2010
  • This study was conducted to assess the effects of organic and inorganic copper on performance in growing pigs. A total of 100 pigs, average age 63 d and initial body weight 21.46${\pm}$1.13 kg, were assigned to five treatment groups. Dietary treatments included i) CON (basal diet, 0 ppm Cu), ii) T1 (basal diet with 67 ppm Cu as cupric sulfate, $CuSO_4$), iii) T2 (basal diet with 134 ppm Cu as $CuSO_4$), iv) T3 (basal diet with 67 ppm Cu as cupric methionate, CuMet) and v) T4 (basal diet with 134 ppm Cu as CuMet). Throughout the entire experimental period, ADG (average daily gain), ADFI (average daily feed intake) and G/F (gain: feed) ratios showed no significant differences. The dry matter digestibility was improved in the T1, T2, T3, and T4 treatments (p<0.05), as compared with CON. Nitrogen digestibility was improved in the T3 treatment group as compared with CON (p<0.05). As compared with the T1 treatment group, fecal pH values were improved in the CON, T3, and T4 treatment groups (p<0.05). Fecal Cu concentrations were significantly lower in the CON, T3, and T4 treatment groups than in T1 and T2 (p<0.05). The incidence of diarrhea was reduced when the pigs were fed on the T2, T3, and T4 diets as compared with CON. In conclusion, diets supplemented with 67 or 134 ppm Cu as CuMet may prove effective in improving nutrient digestibility and fecal pH value in growing pigs, and fecal Cu concentrations may be reduced by CuMet supplementation.

Cyclic Deformation Behaviors under Isothermal and Thermomechanical Fatigue Conditions in Nb and Mo Added 15Cr Ferritic Stainless Steel (Nb 및 Mo 첨가 페라이트계 스테인리스강의 등온 저주기 및 열기계적 피로에 따른 변형거동)

  • Jung, Jae Gyu;Oh, Seung Taik;Choi, Won Doo;Lee, Doo Hwan;Lim, Jong Dae;Oh, Yong Jun
    • Korean Journal of Metals and Materials
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    • v.47 no.11
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    • pp.707-715
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    • 2009
  • This paper deals with cyclic stress and strain responses during isothermal low cycle fatigue (LCF) and thermo-mechanical fatigue (TMF) loadings on Nb and Mo containing 15Cr stainless steel, which is used for exhaust manifolds in automobiles. The test temperatures ($T_{i}$) of the isothermal LCF were 600 and $800^{\circ}C$. The minimum temperature of the TMF test was $100^{\circ}C$ and the maximum temperaures ($T_{p}$) were varied between 500 and $800^{\circ}C$. In both loading conditions, weak cyclic softening is observed at $T_{i}=T_{p}=800^{\circ}C$, but the transition to strong cyclic hardening is completed with the temperature decrease below $T_i=600{\sim}700^{\circ}C$ for LCF and $T_{p}=500{\sim}600^{\circ}C$ for TMF. The stress-strain hysteresis loops in the TMF loading show a significant stress relaxation during compressive (heating) half cycle at $T_{p}>500^{\circ}C$, which develops tensile mean stress during cycling. Due to the stress relaxation, the TMF test sample reveals much lower dislocation density than the isothermally fatigued sample at the same temperature with $T_{p}$. A detailed correlation between fatigue microstructure and cycling deformation behavior is discussed.

Cross-Correlation Distribution of a p-ary m-Sequence Family Constructed by Decimation (Decimation에 의해 생성된 p-진 m-시퀀스 군의 상호 상관 값의 분포)

  • Seo, Eun-Young;Kim, Young-Sik;No, Jong-Seon;Shin, Dong-Joon
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.33 no.9C
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    • pp.669-675
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    • 2008
  • For an odd prime p, n=4k and $d=((p^2k+1)/2)^2$, there are $(p^{2k}+1)/2$ distinct decimated sequences, s(dt+1), $0{\leq}l<(p^{2k}+1)/2$, of a p-ary m-sequence, s(t) of period $p^n-1$. In this paper, it is shown that the cross-correlation function between s(t) and s(dt+l) takes the values in $\{-1,-1{\pm}\sqrt{p^n},-1+2\sqrt{p^n}\}$ and their, cross-correlation distribution is also derived.

Cloning and Expression of D-amino Acid Oxidise from Trigonopsis variabilis for Cephalosporin C Biotransformation (Cephalosporin C의 생변환을 위한 Trigonopsis variabilis의 D-amino Acid Oxidase 유전자의 클로닝 및 발현)

  • 이진형;정태완
    • KSBB Journal
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    • v.10 no.3
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    • pp.264-270
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    • 1995
  • Trigonopsis variabilis is a strong producer of D-amino acid oxidase that can transform cephalosporin C(ceph C) to ${\alpha}$-keto-adipyl-7-aminocephalosporanic acid(AKA-7ACA). Polymerase chain reaction (PCR) was applied to isolate the D-AAO gene from T. variabilis. To clone the PCR fragment, four different methods were examined using enzymatic reactions of Taq DNA polymerase, Klenow, T4 DNA polymerase I, Alkaline phosphatase Calf Intestinal, and T4 kinase. Ligation of phosphorylated blunt-end PCR fragment and dephosphorylated blunt-end of pUC18 plasmid yielded the best cloning efficiency One of recombinant E. coli transformants showed D-AAO activity against ceph C in both cell extracts and permeabilized cells.

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Structural dependences of the extinction in an 1.55 $1.55{\mu}m$ InGaAsP/InGaAsP multiple-quantum-well electro-absorption modulator (1.55 $1.55{\mu}m$ InGaAsP/InGaAsP MQW 광흡수 변조기에서 구조변수가 소광특성에 미치는 영향)

  • 민영선;심종인;어영선
    • Korean Journal of Optics and Photonics
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    • v.12 no.1
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    • pp.40-47
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    • 2001
  • The structural dependence of the performance of an 1.55 $1.55{\mu}m$ InGaAsPIInGaAsP MQW electro-absorption modulator for highspeed digital fiber communication was systematically investigated. The effects of n-doped SCH region length $t_n$ as well as the general structure parameters including quantum well number $N_w$, well-thickness $t_w$, detuning wavelength $\Delta\lambda$, and device length L were thoroughly analyzed. Thereby, a high-pelfoIDlance electro-absorption modulator with device length L of $100{\mu}m$ was successfully designed. The designed structure showed excellent characteristics that have residual loss less than -1.5 dB, operational voltage from 0 V to -2V, and extinction ratios of -2.92 dB at $V_{\alpha}$=-1 V and -10 dB at $V_{\alpha}$=-2V.X>=-2V.

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Study on the Determination of Crude Protein Requirement for Maintenance of fattening Black Goat(Capra hircus coreanae) (비육기 흑염소의 유지를 위한 조단백질 요구량 결정에 관한 연구)

  • Yun, Yeong Sik;Jang, Se Young;Seong, Hye Jin;Tang, Yu Jiao;Ding, Yu Ling;Park, Jae Hyun;Moon, Sang Ho
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.2
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    • pp.176-182
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    • 2017
  • This study was conducted to evaluate the protein requirement for maintenance of fattening Korean black goat (Capra hircus coreanae). Six male goats with average initial body weight (BW) of $31.78{\pm}4.54kg$ and an average age of 8 months were used in this study. The experiment had a replicated duplicated $3{\times}3$ Latin square design for balancing carryover effects. In the course of the experiment, each of Black goats were fed three diets that were formulated to contain T1 (13%), T2 (16%) and T3 (19%) levels of crude protein (CP). A 14-day diet adjustment period was followed by a 5-day collection period. Dry matter intake (DMI) of groups fed diets with T2 was 966.67g/d which was higher than group fed diets with T1 and T3 were 925.14g/d and 936.08g/d each. Average daily gains (ADG) of black goats were the highest in T2(167.13g/d) But, there was no significant difference. Dietary protein levels affected the apparent digestibility of CP (p<0.05). A significant difference was found in CP intake among treatments and goats receiving T3, T2, and T1 recorded 181.23, 154.57, and 128.78g CP/d, respectively. This was excepted because CP intake is proportional to CP content of diet, which from highest to lowest was as follows: T3 (19%) > T2 (16%) > T1 (13%). Intercept of the regression equation between CP intake and CP balance indicated that maintenance CP requirement was 1.63g/BW0.75.

Influence of Pre-operative Fasting Time on Blood Glucose in Older Patients (수술 전 금식이 노인환자의 혈당에 미치는 영향)

  • Hong, Mi-Suk;Yoon, Hae-Sang
    • Journal of Korean Academy of Nursing
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    • v.41 no.2
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    • pp.157-164
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    • 2011
  • Purpose: This study was performed to identify changes in blood glucose at preoperative fasting time in surgical patients over 60 yr. Methods: Data collection was performed from July, 2008 through July, 2009. Participants consisted of 80 nondiabetic surgical patients. Blood glucose was checked from 3 to 5 times. The 5 times were 2-hr fasting on the pre-operative day (T1, n=80), 8 hr (T2, n=80), 10 hr (T3, n=17), 12 hr (T4, n=34) and 14 hr fasting on the day of the operation (T5, n=29). Results: Of the patients, 27.5% had a blood glucose level of less than 79 mg/dL at T2; 17.6% at T3; 32.4% at T4; and 17.2% at T5. Mean blood glucose levels were 93.8 mg/dL at T1; 88.4 mg/dL at T2; 91.7 mg/dL at T3; 87.4 mg/dL at T4: and 94.1 mg/dL at T5. Blood glucose was the lowest at T2 (p<.001). Conclusion: As 17.6-32.4% of the patients showed the blood glucose level of less than 79 mg/dL at 8-14 hr pre-operative fasting, the authors recommend that surgical patients p>60 yr-of-age be observed for hypoglycemia during pre-operative fasting of more than 10 hr and that surgical patients >60 yr-of-age with risks for hypoglycemia be scheduled for operation within 10 hr preoperative fasting.

NOTE ON THE OPERATOR ${\hat{P}}$ ON Lp(∂D)

  • Choi, Ki Seong
    • Journal of the Chungcheong Mathematical Society
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    • v.21 no.2
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    • pp.269-278
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    • 2008
  • Let ${\partial}D$ be the boundary of the open unit disk D in the complex plane and $L^p({\partial}D)$ the class of all complex, Lebesgue measurable function f for which $\{\frac{1}{2\pi}{\int}_{-\pi}^{\pi}{\mid}f(\theta){\mid}^pd\theta\}^{1/p}<{\infty}$. Let P be the orthogonal projection from $L^p({\partial}D)$ onto ${\cap}_{n<0}$ ker $a_n$. For $f{\in}L^1({\partial}D)$, ${\hat{f}}(z)=\frac{1}{2\pi}{\int}_{-\pi}^{\pi}P_r(t-\theta)f(\theta)d{\theta}$ is the harmonic extension of f. Let ${\hat{P}}$ be the composition of P with the harmonic extension. In this paper, we will show that if $1, then ${\hat{P}}:L^p({\partial}D){\rightarrow}H^p(D)$ is bounded. In particular, we will show that ${\hat{P}}$ is unbounded on $L^{\infty}({\partial}D)$.

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Effective Reservoir Management Methods using Nutrients Leaching Characteristic Analysis: Case Study of the Hongdong Reservoir (홍동저수지 퇴적토의 유기물 용출 특성 분석을 통한 효율적인 저수지 관리 방안 연구)

  • Lee, Young-Shin;Shin, Sang-Hee
    • The Journal of Engineering Geology
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    • v.23 no.2
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    • pp.95-104
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    • 2013
  • This study proposes an efficient management plan for improving the water quality of agricultural reservoirs. Hongdong reservoir is located in an area that includes farmland, forest, residential housing, and livestock farms. The levels of pollutants in Hongdong reservoir were investigated with the aim of improving the water quality in the reservoir. The potential concentrations of total nitrogen (T-N) and total phosphorus (T-P) in Hongdong reservoir were 1.06-3.67 and 0.52-1.12 $g/m^2-d$, respectively. An analysis of leaching characteristics was performed under anaerobic conditions for 72 hours at $20^{\circ}C$. The concentrations of T-P and $PO_4$-P were measured by general leaching for a set period. In the case of T-P, the leaching was measured by irregular leaching due to denitrification of nitrite nitrogen ($NO_2$-N) and nitrate nitrogen ($NO_3$-N). A very small amount of $NH_3$-N on nitrogen ($N_2$) was measured due to the low pH in the column, as a consequence of the anaerobic conditions.