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Antifungal Activity of Methanolic of Centella asiatica and Andrographis panicuiata

  • Singh, Pratibha;Singh, U.P.;Singh, J.S.
    • Mycobiology
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    • 제28권4호
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    • pp.185-189
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    • 2000
  • The antifungal activity of methanolic extracts of Centella asiatica and Andrographis paniculata leaves was observed against fourteen fungi, viz., Alternaria alternata, A. brassicae, A. brassicicola, A. solani, A. tenuissima, Cercospora blumae, Curvularia lunata, C. penniseti, and Drechslera monoceras, D. oryzae, D. turitica, Fusarium albizziae and F. udum. Different concentrations of the methanolic extract (1000, 2000, 3000, 5000, 7000, 10000ppm) were used. The effect of mixed leaf extract (1500 ppm of C. asiatica + 1500 ppm of A. paniculata) and its 1:2 ad 1:4 dilutions were also studied. The individual extracts of both the plants showed significant inhibitory effect on spore germination of all the fungi tested. F. udum, F. albizzae, D. oryzae, D. turtica, and D. monoceras were particularly sensitive to these extracts. In general, the extract of C. asiatica showed a higher inhibitory effect in all concentrations against all the fungi as compared to A. paniculata, except for A. brassicae A. solani, D. oryzae, D. penniseti and Curvularia sp. The inhibitory effect of extracts increased when they were used in combination with or without dilutions against A. brassicicola, A. solani A. brassicae, A. alternata, A. tenussima, C. blumae, C. lunata, C. penniseti and Curvularia species. Higher efficacy of active ingredient of these extracts under field condition is envisaged against plant pathogens.

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Development of Aerosol Retrieval Algorithm Over Ocean Using FY-1C/1D Data

  • Xiuqing, Hu;Naimeng, Lu;Hong, Qiu
    • 대한원격탐사학회:학술대회논문집
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    • 대한원격탐사학회 2003년도 Proceedings of ACRS 2003 ISRS
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    • pp.1255-1257
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    • 2003
  • This study proposes a single-channel satellite remote sensing algorithm for retrieving aerosol optical thickness over global ocean using FY-1C/1D data. An efficient lookup table (LUT)method is adopted in this algorithm to generate apparent reflectance in channel 1 and channel 2 of FY-1C/1D over ocean. The algorithm scale the apparent reflectance in cloud-free conditions to aerosol optical thickness using a state-of-art radiative transfer model 6S with input of the relative spectral response of channel 1 and 2 of FY-1C/1D. Monthly mean composite maps of the aerosol optical thickness have been obtained from FY-1C/1D global area coverage data between 2001 and 2003. Aerosol optical thickness maps can show the major aerosol source which are located off the west coast of northern and southern Africa, Arabian Sea and India Ocean. These result is very similar to other satellite sensors such as AVHRR and MODIS in the location area of heavy aerosol optical thickness over global ocean. The algorithm have been used to FY-1D operational performance and it is the first operational aerosol remote sensing product in China.

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쌀가루의 제분방법에 따른 증편의 노화도 특성 (Retrogradation Characteristics of Jeungpyun by Different Milling Method of Rice Flour)

  • 김영인;금준석;이상효;이현유
    • 한국식품과학회지
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    • 제27권6호
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    • pp.834-838
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    • 1995
  • 각기 다른 제분방법에 따라 제조한 쌀가루를 이용하여 증편을 제조한 후 노화도 특성을 조사하였다. 증편의 노화도는 DSC측정에서는 전체적으로 W-C, W-P, D-M, D-J제품 순으로 낮게 나타났고, diastase법에서는 W-C, D-M, D-J, W-P순으로 낮게 나타났다 따라서 DSC측정이나 diastase법에 의한 측정 모두 습식제품 중에서는 W-C제품이 W-P제품보다 크고, 건식제품중에서는 D-M제품이 D-J제품 보다 크게 나타나 유사한 경향을 보여주었다. 이는 각 제분 방법에서 쌀가루의 입자크기 및 Amylogram의 setback값이 감소할수록 또는 전분 손상도가 증가할수록 증편의 노화도는 감소하는 것으로 나타났다.

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Mainchain NMR Assignments and secondary structure prediction of the C-terminal domain of BldD, a developmental transcriptional regulator from Streptomyces coelicolor A3(2)

  • Kim, Jeong-Mok;Won, Hyung-Sik;Kang, Sa-Ouk
    • 한국자기공명학회논문지
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    • 제17권1호
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    • pp.59-66
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    • 2013
  • BldD, a developmental transcription factor from Streptomyces coelicolor, is a homodimeric, DNA-binding protein with 167 amino acids in each subunit. Each monomer consists of two structurally distinct domains, the N-terminal domain (BldD-NTD) responsible for DNA-binding and dimerization and the C-terminal domain (BldD-CTD). In contrast to the BldD-NTD, of which crystal structure has been solved, the BldD-CTD has been characterized neither in structure nor in function. Thus, in terms of structural genomics, structural study of the BldD-CTD has been conducted in solution, and in the present work, mainchain NMR assignments of the recombinant BldD-CTD (residues 80-167 of BldD) could be achieved by a series of heteronuclear multidimensional NMR experiments on a [$^{13}C/^{15}N$]-enriched protein sample. Finally, the secondary structure prediction by CSI and TALOS+ analysis using the assigned chemical shifts data identified a ${\beta}-{\alpha}-{\alpha}-{\beta}-{\alpha}-{\alpha}-{\alpha}$ topology of the domain. The results will provide the most fundamental data for more detailed approach to the atomic structure of the BldD-CTD, which would be essential for entire understanding of the molecular function of BldD.

Streptomyces sp.에 의한 포도당 이성화효소의 생성 (Formation of D-Glucose Isomerase by Streptomyces sp.)

  • Rhee, In-Koo;Seu, Jung-Hwn
    • 한국미생물·생명공학회지
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    • 제8권3호
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    • pp.173-180
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    • 1980
  • Streptomyces sp. strain K-17의 포도당 이성화효소의 강력한 분비를 위해서는 inducer로서 D-xylose를 필요로 하고 있다. 그런데 D-xylose를 가하지 않고 1.0% glucose를 가한 배지에서 배양한 이성화효소 역가가 낮은 균체를 모아서 이것을 다시 0.05M인 산 완충액 (PH7.0)에 현탁시켜 0.5 % xylose를 가하여 호기적으로 해주었을 때 효소의 induction pattern을 조사한 결과 효소활성이 10시간까지는 처리 시간에 따라 직선상으로 증가하고 이에 비례해서 D-xylose의 양이 감소했으나 cell mass에 있어서는 거의 변동이 없었다. 이때 효소단백의 합성이 일어나고 있지만, 전 RNA함량에 있어서는 오히려 감소하였다. 이와 같이 질소원을 가하지 않는 non-growth phase에서도 효소단백의 합성이 일어나는 것은 세포내에 축적되어 있는 단백질의 turn-over에 의한다는 것을 starvation 실험에서 알 수 있었다. D-xylose 이외에 D-ribose, L-arabinose, D-glucose, D-mannose, citrate, succinate 및 tartrate는 inducer로서의 효과가 없었다. 효소의 induction시, D-glucose를 가했을 경우catabolite repression 이 일어났으며 succinate 나 citrate 에 의해서도 강하게 효소생성이 억제되었다. 이와 같은 현상은 growth phase에서도 마찬가지 결과를 나타내었다. Induction시, $Ba^{2+}$, $Mg^{2+}$$Co^{2+}$가 효소생성을 발전시켰으며, C $u^{2+}$, C$d^{2+}$, A $g^{+}$ 및 H $g^{2+}$ 와 같은 중금속이 효소생성을 저해하였고, mitomycin C 몇 penicillin G는 효소생성에 영향을 주지 못하였으나, actinomycin D, streptomycin, chlora-mphenicol 및 tetra cycline등에 의해 강하게 저해되었다. 또 p-CMB 및 uncoupler 인 arsenate와 2.4-DNP에 의해서도 효소생성이 저해되었다.

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Alditol Acetates의 분자구조 (The Structures of Alditol Acetates)

  • 박영자;박명희;신정미
    • 대한화학회지
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    • 제34권6호
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    • pp.517-526
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    • 1990
  • D-glucitol hexaacetate 와 xylitol pentaacetate의 결정구조를 단결정 X-선 회절법으로 연구하였다. D-glucitol hexaacetate 는 공간군 P2$_1$, 단위세포 상수는 a = 10.275 (2), b = 8.363 (1), c = 12.560 (5) $\AA$, $\beta$= 95.97 $(2)^{\circ}$; Z = 2이고, xylitol pentaacetate는 공간군이 P2$_1$/C이고, a = 18.126 (1), b = 11.422 (2), c = 8.649 (1) $\AA$, $\beta$ = 95.03 $(1)^{\circ}$이며 Z = 4이다. 회절세기는 diffractomerer로 얻었고, 분자구조는 직접법으로 밝혔으며, 최소자승법으로 정밀화하였다. 두 분자에 결쳐 결합의 평균길이는 C($sp^3)-C(sp^3$)가 1.514 (10), C($sp^3)-O: 1.444 (6),\; C(sp^2)-O: 1.347 (9),\; C(sp^2)=O: 1.197 (6),\; C(sp^2)-C(sp^3): 1.479(9){\AA}$이고, 평균 결합각도는 C($sp^3)-C(sp^3)-C(sp^3): 114.6 (17),\; O-C(sp^3)-C(sp^3): 109.4 (23),\; C(sp^2)-O-C(sp^3): 117.4 (6),\; O=C(sp^2)-O: 122.6 (6),\; C(sp^3)-C(sp^2)-O: 111.8 (7), C(sp^3)-C(sp^2)=O: 125.5 (4)${\circ}$이다. 두 분자내의 탄소 사슬들은 extended zigzag chain으로 되어 있어 D-glucitol과 xylitol 분자들의 conformation과는 다르다. Acetate group들의 각 원자들은 모두 한 평편상에 놓여있다.

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Bacillus subtilis C4와 B. cereus D8에 의한 유채의 생육증대 및 무름병과 균핵병 방제효과 (Effect of Bacillus subtilis C4 and B. cereus D8 on Plant Growth of Canola and Controlling Activity Against Soft Rot and Stem Rot)

  • 이재은;이서현;박경수;박진우;박경석
    • 농약과학회지
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    • 제13권4호
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    • pp.275-282
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    • 2009
  • 선발된 PGPR 균주인 Bacillus subtilis C4와 Bacillus cereus D8 균주의 유채에 대한 생육촉진 및 무름병균인 Erwinia carotovora와 균핵병균인 Sclerotinia sclerotiorum에 대한 방제효과를 검정하기 위하여 실내검정과 온실검정을 실시하였다. 실내검정 결과, C4와 D8균주처리에 의하여 유채의 생육이 40.3%~74% 증가하였으며 무름병이 대조구에 비하여 80% 감소하였다. 실내검정에서 C4와 D8균주를 종자에 처리하였을 때 뿌리가 크게 신장되었다. 주요 식물병원균 Sclerotinia sclerotiorum, Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum, Phytophthora capsici, Colletotrichum acutatum에 대하여 항균활성시험을 수행한 결과 두 균주 중 C4균주는 모든 병원성 곰팡이에 대하여 항균활성을 나타내었다. 온실검정에서 C4와D8균주처리는 대조구에 비하여 유채의 초장, 엽폭 및 엽장을 19.5%~24.9%, 11.3%~15.3%, 14.1%~20.7% 각각 증가시켰으며 균핵병균인 Sclerotinia sclerotiorum에 대한 억제효과가 우수하였다. 이와 같은 결과를 볼 때 C4, D8 균주 처리는 유채의 생육을 촉진시키며 유채에 저항성을 유도하므로 친환경생물방제에 적용할 수 있을 것으로 생각된다.

The Development of C&DH subsystem in the D-SAT System

  • Zenaida Awalludin Sitti;Mat Yusoff Norhan
    • 대한원격탐사학회:학술대회논문집
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    • 대한원격탐사학회 2004년도 Proceedings of ISRS 2004
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    • pp.39-41
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    • 2004
  • This paper demonstrates the in-house development of the subsystem C&DH in a D-SAT satellite project of Astronautic Technology (M) Sdn. Bhd. (ATSB). D-SAT is a non-imaging satellite. It will carry a dosimeter as the payload for scientific objectives. Since one of the D-SAT project establishment intended objectives is to demonstrate the research and development skill of the ATSB engineers on the satellite system building, most of the subsystems of the D-SAT are developed in-house including the C&DH subsystem. C&DH subsystem plays an important role for managing data handling tasks and general operation of the satellite system. The C&DH development includes the research and study on the hardware and software design, the design of both the hardware and software, the development of the software system, the integration between the software and the hardware system and the implementation of the subsystem into the satellite system. This program allows ATSB to accomplish the engineers with expertise not only in the C&DH subsystem but other subsystems too besides developing knowledge in the management of a space project that covers not only on the technical issues but issues relating to the economic, political and legal issues.

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일부 도시지역 영아의 예방접종율에 미치는 요인에 관한 조사연구 (A Study on the Factors Which Influenced Vaccination Rate of Infants in a City)

  • 배미승
    • Journal of Preventive Medicine and Public Health
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    • 제16권1호
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    • pp.89-97
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    • 1983
  • From January to March in 1979 and 1982, the vaccination rate of B.C.G., D.P.T and Sabin with 392 infants who were registered at M.C.H. room in a Health Center in Seoul were as follows: 1 There were no specific relations between the sex and the vaccination for B.C.G., D.P.T. and Polio. 2. In 1982, the younger the mother's age was, the higher the vaccination rate for B.C.G. was. For the D.P.T. and Polio the rate of above 35 year group was the highest but the completion rate of the vaccination and the regular vaccination rate were the highest in the age of 30-34 year group. 3. In 1982. the higher the educational levels of tile mother were, the higher the vaccination rates for B.C.G., D.P.T. and Polio were. 4. The vaccination rate for B.C.G. within a month after birth was the highest in Salaried laborer group. The completion rate of the vaccination and regular vaccination rate for D.P.T. add Polio were also the highest in salaried laborer group. 5. In 1982, the rates of all vaccination for B.C.G. were tile highest in the first child. The completion rate of the vaccination and regular vaccination rate for D.P.T and Polio were also the highest in the first child in 1982.

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음향방출법에 의한 SM45C 고주파 열처리 강의 기계적 특성 평가 (The Analysis of Mechanical Properties of the High Frequency Induction Hardening SM45C Steel by Acoustic Emission)

  • 이장규
    • 한국기계기술학회지
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    • 제13권2호
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    • pp.93-100
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    • 2011
  • This study deals with the high frequency induction hardening (HF at $850^{\circ}C$, 120kHz & 50kW condition) SM45C steel. (1) The HF specimen, which was tempered at $150^{\circ}C$, did not show any tempering effect. A brittle fracture occurred at rounded area of the tensile specimen. AE (acoustic emission) amplitude distribution showed between 45dB and 60dB. (2) A slip and fracture occurred at the hole area of the HF specimen which was tempered at $300^{\circ}C$. As they pass the yield point, the AE energy is increased intermittently and AE amplitude distribution exists between 70dB and 85dB. In addition, after imposing the maximum tensile load, AE signals showed high amplitude and energy distribution. The AE amplitude showed between 45dB and 70dB. (3) A brittle fracture occurred at HF specimen which was tempered at $450^{\circ}C$ as if it is torn in the direction of $45^{\circ}$ on parallel area over the both sides of the tensile specimen, which lead to several peak appeared in AE energy. It was found that the AE amplitude was relatively low and the AE energy was high.