• 제목/요약/키워드: D-loop Region

검색결과 124건 처리시간 0.025초

Evaluating genetic diversity and identifying priority conservation for seven Tibetan pig populations in China based on the mtDNA D-loop

  • Ge, Qianyun;Gao, Caixia;Cai, Yuan;Jiao, Ting;Quan, Jinqiang;Guo, Yongbo;Zheng, Wangshan;Zhao, Shengguo
    • Asian-Australasian Journal of Animal Sciences
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    • 제33권12호
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    • pp.1905-1911
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    • 2020
  • Objective: Tibetan pigs, an excellent species unique to China, face serious threats, which in turn affects the development and utilization of the outstanding advantages of plateau hypoxia adaptability and reduces their genetic diversity. Therefore, a discussion of measures to conserve this genetic resource is necessary. The method, based on genetic diversity, genetic divergence and total genetic contribution rate of population, reflects the priority conservation order and varies depending on the three different purposes of conservation. Methods: We analyzed mitochondrial DNA control region (D-loop) variation in 1,201 individuals from nine Tibetan pig populations across five provinces and downloaded 564 mtDNA D-loop sequences from three indigenous pig breeds in Qinghai, Sichuan, and Yunnan Provinces distributed near the Tibetan pigs. Results: We analyzed three different aspects: Changdu Tibetan pigs have the highest genetic diversity, and from the perspective of genetic diversity, the priority conservation is Changdu Tibetan pigs. Hezuo Tibetan pigs have the highest genetic contribution, so the priority conservation is Hezuo Tibetan pigs in the genetic contribution aspect. Rkaze Tibetan pigs were severely affected by indigenous pig breeds, so if considering from the perspective of introgression, the priority conservation is Rkaze Tibetan pigs. Conclusion: This study evaluated genetic diversity and comprehensively assessed conservation priority from three different aspects in nine Tibetan pig populations.

작은 루우프 안테나의 근거리장 영역내에 있는 다단 연쇄 접속 전송선로에 유기되는 부하 전류 계산 (Calculations of Load Current of Multi-Stage Cascaded Transmission Lines Excited by a Small Loop in the Near-Field Region)

  • 박영태;이상설
    • 대한전자공학회논문지
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    • 제27권6호
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    • pp.836-842
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    • 1990
  • The noise currents in the load of cascaded transmission lines excited by a nearly small loop are analyzed and the effective approxiamte method by which we can calculate these currents easily and the computation time can be significantly reduced is developed. Numerical results for n=3 are well agreed with those of Smith by the maximum difference of 3-4dB and show that the length of a segment of line regarded to be illuminated by uniform electric fields should be 1.3 times the distance from the loop to the point midway between the conductors.

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Backbone assignment of HMGB1 A-box and molecular interaction with Hoxc9DBD studied by paramagnetic probe

  • Choi, Ji Woong;Park, Sung Jean
    • 한국자기공명학회논문지
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    • 제25권2호
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    • pp.17-23
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    • 2021
  • High mobility group protein B1 (HMGB1) is a highly conserved, non-histone, chromatin associated nuclear protein encoded by HMGB1 gene. HMGB1 proteins may be general co-factors in Hox-mediated transcriptional activation that facilitate the access of Hox proteins to specific DNA targets. It is unclear that the exact binding interface of Hoxc9DBD and HMGB1. To identify the interface and binding affinity of Hoxc9DBD and HMGB1 A-box, the paramagnetic probe, MTSL was used in NMR titration experiment. It is attached to the N-terminal end of HMGB1 A-box by reaction with thiol groups. The backbone assignment of HMGB1 A-box was achieved with 3D NMR techinques. The 15N-labeled HMGB1 A-box was titrated with MTSL-labeled Hoxc9DBD respectively. Based on the chemical shift changes we can identify the interacting residues and further map out the binding sites on the protein structure. The NMR titration result showed that the binding interface of HMGB1 A-box is around loop-1 between helix-1 and helix-2. In addition, the additional contacts were found in N- and C-terminus. The N-terminal arm region of Hoxc9DBD is the major binding region and the loop between helix1 and helix2 is the minor binding region.

mtDNA D-loop 염기서열 변이를 이용한 토종오리의 계통 분류 (Phylogenetic Analysis using mtDNA D-loop Sequences in Korean Native Ducks)

  • 최누리;서동원;진선덕;술타나;허강녕;이준헌
    • 한국가금학회지
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    • 제41권4호
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    • pp.235-240
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    • 2014
  • 최근 국내 오리고기 산업이 점진적으로 증가하고 있으나, 국내에서 생산되고 있는 대부분의 오리 종자는 수입에 의존하고 있는 실정이다. 이러한 의존도를 낮추기 위해서 국립축산과학원에서는 토종오리 상용화 연구를 시작하였으며, 이에 토종오리 품종 및 개체 식별의 필요성이 대두되었다. 본 연구에서는 토종오리와 백색실용오리가 다른 야생오리 품종과 얼마나 연관되어 있는지를 알아보기 위해 미토콘드리아 DNA의 D-loop control 영역의 염기서열을 이용하여 계통분석을 수행하였다. 그 결과, 토종오리와 백색실용오리는 대부분의 야생오리 품종과는 구분이 잘 되지만, 청둥오리와는 분류가 되지 않는 것을 확인할 수 있었다. 또한, 토종오리와 백색실용오리 품종간의 분류 또한 이들 집단이 다수의 변이와 haplotype을 공유하고 있음을 확인할 수 있었다. 분석 결과, 11개의 염기 변이가 확인되었으며, 이 변이들은 8개 haplotype으로 구성되어 있었다. 이 중 토종오리에서만 확인된 haplotype 2를 제외한 3개의 haplotype(haplotype number 1, 3, 4)에서 토종오리와 백색실용오리가 동일한 haplotype을 가진 반면, haplotype 5, 6, 7, 8은 백색실용오리에서만 확인되었다. 이상의 결과를 바탕으로 D-loop control 영역은 토종오리와 야생오리의 품종 구분을 위한 기초 자료로 이용될 수 있을 것으로 판단된다.

선형전동기를 적용한 LCD 패널 자동반송용 순환궤도차량 시스템 개발 (Development of OHS System Driven by Linear Motor for Automatic Transfer of LCD Panels)

  • 김원곤;윤종보;박건우;황계호
    • 반도체디스플레이기술학회지
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    • 제7권3호
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    • pp.11-16
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    • 2008
  • The authors investigated an overhead shuttle (OHS) system for automatic transferring the liquid crystal display (LCD) panels. The constructed tracks of OHS system include the linear and curve regions and have been installed on the ceiling to transfer the cassette of LCD glass along the closed-loop and open-loop tracks. In this study, the OHS system was implemented by a proposed linear motor to solve encoder installation and the system cost problems of the long distance transfer system. In addition, we utilized a new algorithm of the position detection and a new control algorithm for driving linear motor. The newly developed control algorithm was demonstrated from both a computer simulation and an experimentation, indicating that the highly reliable and speedy transfer system can enhance the LCD panel productivity of commercial OHS system.

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분할 링 공진기를 이용한 소형 이중 대역 CPW-급전 슬롯 안테나 (Compact Dual-band CPW-fed Slot Antenna Using Split-Ring Resonator)

  • 여준호;박진택;이종익
    • 한국정보통신학회논문지
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    • 제19권11호
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    • pp.2526-2533
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    • 2015
  • 본 논문에서는 분할 링 공진기(SRR; split-ring resonator)를 이용한 소형 이중 대역 코플래너 도파관 급전 슬롯 안테나에 대한 설계 방법을 연구하였다. 제안된 슬롯 안테나의 이중 대역 동작을 위해 SRR 도체를 직사각형 슬롯 내에 장하하였다. 기존의 직사각형 슬롯에 SRR 도체가 삽입되면 직사각형 루프 영역과 직사각형 슬롯 영역으로 나누어지며, 루프와 슬롯에 의해 각각 주파수 대역이 발생한다. 2.45 GHz WLAN 대역과 3.40-5.35 GHz 대역에서 동작하는 이중 대역 슬롯 안테나를 FR4 기판 상에 $30mm{\times}30mm$ 크기로 제작하였다. 실험 결과, 전압 정재파비 < 2인 대역이 2.38-2.51 GHz, 3.32-5.38 GHz으로 원하는 이중대역에서 동작하는 것을 확인하였고, 이득은 2.45 GHZ에서 1.7 dBi이고 두 번째 대역에서는 2.4-3.2 dBi로 측정되었다.

Determination of Regulator Parameters and Transient Analysis of Modified Self-commutating CSI-fed IM Drive

  • Pandey, A.K.;Tripathi, S.M.
    • Journal of Electrical Engineering and Technology
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    • 제6권1호
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    • pp.48-58
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    • 2011
  • In this paper, an attempt has been made to design the current and speed proportional and integral (PI) regulators of self-commutating current source inverter-fed induction motor drive having capacitors at the machine end and to investigate the transient performance of the same for step changes in reference speed. The mathematical model of the complete drive system is developed in closed loop, and the characteristic equations of the systems are derived using perturbation about steady-state operating point in order to develop the characteristic equations. The D-partition technique is used for finding the stable region in the parametric plane. Frequency scanning technique is used to confirm the stability region. Final selection of the regulator parameters is done by comparing the transient response of the current and speed loops for step variations in reference. The performance of the drive is observed analytically through MATLAB simulation.

Mitochondrial DNA Polymorphism, Maternal Lineage and Correlations with Postnatal Growth of Japanese Black Beef Cattle to Yearling Age

  • Malau-Aduli, A.E.O.;Nishimura-Abe, A.;Niibayas, T.;Yasuda, Y.;Kojima, T.;Abe, S.;Oshima, K;Hasegawa, K.;Komatsu, M.
    • Asian-Australasian Journal of Animal Sciences
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    • 제17권11호
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    • pp.1484-1490
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    • 2004
  • Mitochondrial DNA haplotypes from the displacement-loop (D-loop) region (436 bp) were genotyped and sequenced in Japanese Black beef cattle raised in the same herd. Correlation coefficients between mitochondrial DNA haplotypes, maternal lineage, birth weight, preweaning average daily gain, weaning weight, post weaning average daily gain and yearling weight were computed. The objective was to study the relationship between maternal and postnatal growth traits and to investigate if postnatal growth of calves to yearling age could be accurately predicted from mitochondrial DNA haplotypes. Results of the phylogenetic analysis revealed 17 maternal lineages and four mitochondrial DNA haplotypes. There were strong, positive and highly significant (p<0.001) correlations among maternal traits ranging from 0.52 to 0.98. Similarly, among postnatal growth traits, most of the correlations were also strong, positive and highly significant (p<0.001); the highest correlation of 0.94 was between preweaning average daily gain and weaning weight. However, correlations between mitochondrial DNA haplotypes and postnatal growth traits were very low, mostly negative and non-significant (p>0.05) ranging from -0.05 to 0.1. Prediction of postnatal growth from mitochondrial DNA yielded very low $R^{2}$ values ranging from 0.002 to 0.019. It was concluded that mitochondrial DNA polymorphism has no significant association with postnatal growth from birth to yearling age, and by implication, nuclear rather than cytoplasmic DNA, accounts for most of the genetic variation observed in postnatal growth of Japanese Black cattle. Therefore, mitochondrial DNA genotyping at an early age has no bearing on the accurate prediction of the future growth performance of calves.

Xanthomonas celebensis 5S rRNA의 몇 가지 삼차상호작용 (Some Tertiary Interactions in 5S rRNA from Xanthomonas celebensis)

  • 조봉래;이영훈;최명언;박인원
    • 대한화학회지
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    • 제37권2호
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    • pp.237-243
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    • 1993
  • Xanthomonas celebensis 5S rRNA를 분리 정제하여 효소적 방법과 화학적 방법으로 그 일차구조 및 이차구조를 결정하였다. 이 5S rRNA는 119개의 누클레오티드로 구성되어 있으며 변형된 누클레오시드를 함유하고 있지 않다. 그리고 이 5S rRNA는 X. maltophilia 및 X. citri의 것처럼 5'-말단에 가외의 우리딘잔기를 하나 더 가지고 있다. 결정한 X. celebensis 5S rRNA의 이차구조는 본 연구진이 이미 결정한 두가지 Xanthomonas 종들의 것들과 매우 유사하며, 5개의 이중나선 줄기와 5개의 단일가닥 고리 그리고 2개의 내밀린 구조를 가지고 있다. X.celebensis 5S rRNA 분자 내의 삼차상호작용은 테옥시헥사머를 이용한 혼성체화법과 Fe(II)-EDTA를 사용하여 5S rRNA를 쪼개는 방법으로 분석하였다. 5S rRNA 상의 $U_{35}CCCAU_{40}$부분을 상보성을 가진 데옥시헥사머를 혼성체화한 다음에 고리 M, 구역 $I_1-C$, 고리 $H_2$에 있는 약간의 아데노신 잔기들이 피로탄산 이에틸에 대한 감수성을 가지게 되는 사실과, $Mg^{2+}$이 있는 조건에서 고리 M, 고리 $H_1$, 및 구역 $D-I_2에$ 있는 약간의 누클레오티드 잔기들이 Fe(II)-EDTA의 분열작용에 저항성을 나타내는 사실에서 고리 $H_1과$ 고리 $H_2$가 어떤 방법으로든 고리 M과 삼차상호작용을 하는 것으로 추측되며, 이 삼차작용에서 구역 $I_1-C$와 구역 $D-I_2$ 돌쩌귀로 작용하는 것으로 생각된다. 고리 $H_1$은 산성 조건(pH 5.5)에서 비표준형 염기쌍 A:C 들을 형성함으로써 3개의 염기로 이루어지는 고리를 형성한다는 것을 알았다.

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Genetic Diversity of mtDNA D-loop Polymorphisms in Laotian Native Fowl Populations

  • Kawabe, K.;Worawut, R.;Taura, S.;Shimogiri, T.;Nishida, T.;Okamoto, S.
    • Asian-Australasian Journal of Animal Sciences
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    • 제27권1호
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    • pp.19-23
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    • 2014
  • Here, we studied the genetic diversity of native fowls in Laos by analyzing a mitochondrial DNA (mtDNA) sequence polymorphism. A 546-bp fragment of the mtDNA D-loop region was sequenced in 129 chickens from the areas of Vientiane, Luang Prabang and Pakse. In total, 29 haplotypes were identified and formed five clades. Haplotype diversity and nucleotide diversity of the native fowls in Laos were $0.85536{\pm}0.0172$ and $0.010158{\pm}0.005555$, respectively. Although the Laotian native fowls were distributed across five clades, most of them were clustered in two main clades (A and B), which were originated in China. The other haplotypes were contained in clades D, F, and I, which originated from continental southeast Asia. These results suggest that multiple maternal lineages were involved in the origin of domestic chicken in Laos. Moreover, there appear to be at least two maternal lineages, one from China and the other from the southeast Asian continent.