• Journal of Plant Biotechnology
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• v.38 no.4
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• pp.258-262
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• 2011

Embryogenic callus was induced from the cultures of immature embryos of Camellia japonica L. on Murashige & Skoog's (MS) solid medium supplemented with 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D), and then the embryogenic callus was proliferated on same medium for 4 weeks over. The embryogenic callus was sub-cultured on MS basal medium without 2,4-D to produce coyledonary stage of somatic embryo. The frequency (%) of somatic embryogenesis was 25.1%, and the majority of somatic embryos formed had a abnormal morphology with cupshaped cotyledon (48.3%), one cotyledon (12.6%), three cotyledons (9.4%), four cotyledons (1.9%), whereas was only normal morphology with two cotyledon (27.5%). When the somatic embryos with normal or abnormal cotyledons transfer to MS basal medium or $\frac{1}{2}$ MS medium with/or without plant growth regulators ($GA_3$, IBA) for regeneration, the frequency (%) of two-cotyledon embryos regenerated into plantlets was higher 11.1% than one cotyledon (0.0~8.3 %), three cotyledons (0.0~5.8%), four cotyledons (0.0%), cup-shaped (0.3~4.2%). These results demonstrated that the anomalous cotyledons of somatic embryos could caused to decrease the rate of plant regeneration.

• Geophysics and Geophysical Exploration
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• v.9 no.1
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• pp.44-49
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• 2006

In this study, the resolution capabilities of electrical resistivity tomography (ERT) in the monitoring of $CO_2$ injection are investigated. The pole-pole and bipole-bipole electrode configuration types are used between two uncased boreholes straddling the $CO_2$ plume. Forward responses for an initial pre-injection model and three models for subsequent stages of $CO_2$ injection are calculated for the two different electrode configuration types, noise is added and the theoretical data are inverted with both L1- and L2-norm optimisation. The results show that $CO_2$ volumes over a certain threshold can be detected with confidence. The L1-norm proved superior to the L2-norm in most instances. Normalisation of the inverted models with the pre-injection inverse model gives good images of the regions of changing resistivity, and an integrated measure of the total change in resistivity proves to be a valid measure of the total injected volume.

• Applied Biological Chemistry
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• v.43 no.3
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• pp.184-189
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• 2000

Callus was induced from the petioles of scented-geranium (Pelargonium graveolense) in MS medium containing various concentrations of plant growth regulators. The highest frequency of more than 70% of callus was induced in 2 mg/l NAA and 0.5 mg/l BAP or 2 mg/l 2,4-D and 0.5 mg/l BAP combined treatment, while not in 2,4-D, NAA or BAP alone. When the callus was transferred to the MS medium containing 0.05 mg/l NAA and 0.5 mg/l BAP, were highest intensity of shoot formation, 14 shoots/callus, was induced after 5 weeks. The highest rooting was observed on hormone-free rooting media from the regenerated shoots after 3 weeks, indicating that the regeneration from geranium callus might be possible by changing the hormone ratios. Peroxidase (POD) specific activities in callus induced from 2 mg/l NAA and 0.5 mg/l BAP were higher than those of 2 mg/l 2,4-D and 0.5 mg/l BAP callus during the entire culture periods. POD isozyme C3 was the main cathodic POD isozyme expressed in NAA and BAP callus, while C1 was the main in 2,4-D and BAP callus. However, anodic POD isozymes, A1, A2 and A3 were expressed with similar activities in both hormone combinations.

• Applied Biological Chemistry
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• v.30 no.1
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• pp.17-23
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• 1987

Soy milk prepared from soy protein concentrate was fermented with each of the following lactic acid bacteria: Lactobacillus acidophilus KFCC 12731, L. acidophilus AKU 1122, L. bulgaricus, L. casei, Leuconostoc mesenteroides and Streptococcus lactis. The effects of methionine supple mented to soy milk on the growth and acid production by each organism. were investigated. L-methionine reduced the acid production by two strains of L. acidophilus while it had no apparent. effects on the other test cultures. The inhibitory effects of L-methionine on L. acidophilus KFCC 12731 was greater than on L. acidophilus AKU 1122. The acid production by L. acidophilus KFCC 12731 was also reduced substantially by DL-methionine supplemented to soy milk while it was not affected by D-methionine. Supplementation of L-cysteine to soy milk resulted in slight reduction of acid production by L. acido philus KFCC 12731.

• 전기의세계
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• v.26 no.1
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• pp.77-81
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• 1977

This paper aims to develope a new algorithm to overcome the disadvantages of the conventional E.L.D system based on the B-Constants and Penalty-Factors scheme. The main features of this paper are that the Variabiable Decoupled Method usually employed in the Load-Flow studies is introduced to the E.L.D. algorithm developed by Sasson, using the Powell's Nonlinear Programming Scheme. Besides this, other minor refinements are made to reduce memory spaces and computing time. Case studies show that the method suggested here has the remarkable advantages of computing efficiency and memory requirements over Sasson's.

• Journal of Korean Society of Environmental Engineers
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• v.38 no.11
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• pp.618-626
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• 2016

The influence of ferric ion ($Fe^{+3}$) on bioelectrochemical anaerobic digestion for sewage sludge was explored at ambient temperature ($25^{\circ}C$). Before the addition of ferric ion, the removal of volatile solids (VS) was 65.9% and the specific methane production rate was 370 mL/L/d. After the addition of ferric ion (200 ppm) to feed sludge, the bioelectrochemical anaerobic digester was more stable in the state variables including pH, alkalinity, COD and VFAs, and the VS removal and specific methane production rate were increased to 69.8% and 396 mL/L/d, respectively. However, the methane content in biogas was slightly reduced by the addition of ferric ion, indicating that the activity of planktonic anaerobic bacteria (PAB) was more improved after the addition of ferric ion. The dominances of syntrophic bacteria (Cloacamonas) and hyrolytic bacteria (Saprospiraceae, Ottowia pentelensis) in the PAB were increased by the addition of ferric ion. The addition of ferric ion improved the performance of bioelectrochemical anaerobic digestion for sewage sludge at ambient temperature.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.66-66
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• 2018

국화과(Compositae) 다년생 초본인 산국속(Dendranthema)은 국내 약 13여종이 자생하는 것으로 알려져 있으며, 이 중 감국(D. indicum (L.) Des Moul.)과 산국(D. boreale (Makino) Ling ex Kitam.), 구절초(D. zawadskii var. latilobum (Maxim.) Kitam.)가 주로 차 또는 한약재 등의 원료로 이용되고 있다. 차로 이용되는 꽃은 산국이 감국에 비해 상대적으로 작아서 구분이 가능하지만 시중에는 건조된 형태로 가공 유통되므로 육안으로 구분이 쉽지 않고, 산국 유래 제품들은 국내에서 감국 또는 국화로 혼용해서 표기되어 유통되고 있어 그 기원을 명확히 정립할 필요가 있다. 이에 본 연구는 감국과 산국의 분자유전학적 판별을 위해 DNA 바코드 후보 유전자를 활용하여 염기서열분석으로 확보된 SNP 및 InDel 정보를 바탕으로 CAPS 마커를 개발하고자 수행되었다. 감국과 산국 모두 trnL-trnF intergenic spacer 구간에서 약 1kb의 PCR 산물이 확인되었고, 이들 염기서열에서 분석한 2 SNP 및 3 InDel을 대상으로 CAPS 마커 개발을 위한 제한효소 사이트를 탐색하였다. Gap을 포함한 774bp (감국/산국=A/G) 위치의 SNP에서 BstUI(GC^GC)처리로 CAPS 마커로 전환 가능함이 확인되었고, 이에 감국과 산국의 PCR 산물에 제한효소를 처리한 결과, 제한효소 인식 사이트가 존재하는 산국에서 두 개의 DNA 단편이 확인되었다. 위 결과는 다양한 형태로 가공 유통되는 감국과 산국의 판별을 위한 마커로 활용될 수 있으며, 본 연구에 활용된 기술은 추후 건강기능식품 개발을 위한 원료표준화 확립 연구에 유용할 것으로 판단된다.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2014.11a
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• pp.302-303
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• 2014

유도결합플라즈마 보조 D.C.마그네트론 스퍼터링법을 이용하여 STS316L 위에 Ti-Cr-N 박막의 증착하였으며 크롬과 질소의 함량에 따른 부식특성 변화를 관찰하였다. Ti-Cr-N 박막을 증착시켰을 때는 미처리 STS316L에 비해 더 우수한 부식특성을 보였으며 크롬타겟의 인가전원이 100W 일 때와 질소비가 0.3일 때 가장 우수한 내부식 특성을 나타내었다.

• Childhood Kidney Diseases
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• v.6 no.2
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• pp.188-197
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• 2002

Purpose : Nowadays, drinks, foods and snacks have frequently been intensified with calcium and the insights into the importance of calcium-intake in general has developed in Korea. In this decade, we found the numbers of children who was visited to our hospital for evaluation of hematuria defined with hypercalciuria were increased. So we tried to compare the mean levels of serum calcium, alkaline phosphate, sodium, potassium, chloride, BUN, creatinine, bicarbonate and urinary pH who visited our hospital in 1991, 1992 with in 2000, 2001. Materials and methods : Between January 1991 to December 1992, and between January 2000 to December 2001, each 366 children and 488 children, aged 1 month to 15 years, who presented in our hospital for tonsilectomy and adenoidectomy or for inguinal herniorrhaphy were enrolled in the study, The children in the study were checked the level of serum calcium, alkaline phosphate, sodium, potassium, chloride, BUN, creatinine, bicarbonate and urinary pH with the machine which was corrected the similar levels of practical chemical levels in serum. We compared each mean levels in 1991s' group with in 2001s' group totally and separately through the age and sex. We used t-test to analysis data. Results : The levels of serum calcium, alkaline phosphate, creatinine, sodium, potassium, and urinary pH of 2001s' group were significantly higher than the levels of 1991s' group(P<0.05). The each level was $9.91{\pm}0.50\;mg/dL,\;248.58{\pm}94.98\;U/L,\;0.61{\pm}0.14\;mg/dL,\;138.64{\pm}2.22\;mM/L,\;4.35{\pm}0.40\;mM/L,\;6.18{\pm}0.86$ in 2001s' and $9.13{\pm}0.68;mg/dL,\;198.26{\pm}79.34\;U/L,\;0.433{\pm}0.18\;mg/dL,\;137.86{\pm}2.67\;mM/L,\;4.22{\pm}0.36\;mM/L,\;5.83{\pm}0.95$ in 1991s'. And the levels of serum bicarbonate, $23.64{\pm}2.57\;mM/L$ in 2001s' was significantly lower than the 1991s', $24.60{\pm}2.23\;mM/L$(P<0.05). The similar results were detected each age and sex group. Conclusion : The levels of serum calcium increase in this decade. The results will be used as a basic data for the national health plan in the years to come.

• Journal of Mushroom
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• v.19 no.4
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• pp.300-309
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• 2021

This study was conducted to develop food and medicinal products containing useful components of Lentinula edodes in Codonopsis lanceolata and Platycodon grandiflorus for use as herbal medicine. We manufactured C. lanceolata (FCLM) and P. grandiflorus (FPLM) extract fermented with L. edodes mycelium. The effect of the two fermented products on proximate composition, free sugar, organic acid, 𝛽-glucan, ergothioneine, ergosterol, and vitamin D₂ levels, and 3T3-L1 preadipocyte cell growth were studied. The proximate composition analysis results showed that the crude fiber and crude fat content in FCLM was higher than that in FPLM, and the crude protein and soluble nitrogen content in FPLM was higher than that in FCLM. Free sugar analysis detected arabinose, glucose, and sucrose in both FCLM and FPLM, and the total free sugar content was high in FPLM. The organic acid content was lower in FCLM and FPLM compared to C. lanceolata and P. grandiflorus before fermentation. The 𝛽-glucan content was higher than that of L. edodes used as a control in both fermented products, FCLM and FPLM. The content of ergothioneine, an antioxidant, was higher in FCLM than in FPLM. Ergosterol content was highest in L. edodes which was used as a control, and the two fermented products showed similar content. Vitamin D₂ was detected only in FCLM and FPLM, and FPLM (0.58±0.01 mg%) showed a higher vitamin D₂ content than FCLM (0.47±0.01). FCLM and FPLM showed a higher level of cell viability for 3T3-L1 pre-adipocytes compared to non-fermented C. lanceolata and P. grandiflorus. In addition, FCLM and FPLM inhibited 3T3-L1 preadipocyte differentiation more than C. lanceolata and P. grandiflorus before fermentation, which may exert an anti-obesity effect.