• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.15 no.1
• /
• pp.37-50
• /
• 1989

The in Vitro chemosensitivity of fibroblast cell strains was determined using a semiautomated tetrazolium-based colorimetric assay(MTT assay) to 16 cosmetic materials. This assay is useful method to evaluate toxic effects of the chemicals. From assay results, we determined that the preservatives are more toxic than moisteurizers. The chemicals in the same group have a different toxicity. That is, in preservatives, Germall -115 is more toxic than Danisol -M, -p, and in surfactant sodium laurel sulfate than Myrj 52, and in moisteurizers, 1, 3-butylene glycol is more safe than the others. When the results from this assay for preservatives were compared with patch test results, good correlation was observed. Forthemore, this assay method can be used together with Patch test for the evaluation of the chemical toxicity, particularly in cosmetic field.

• Toxicological Research
• /
• v.6 no.1
• /
• pp.29-40
• /
• 1990

Cytotoxic effects of cytosine arabinoside and vinblastine on cultured fibroblasts were determined by colorimetric assays of neutral red (NR) and tetrazolium MTT, and by mutagenicity tests . Cytosine arabinoside and vinblastine were highly toxic by showing that concentrations of NR-50 and MTT-50 of two drugs were lower than 100 ${\mu}$M. At mid-point cytotoxicityvalue of two drugs, frequencies of micronuclei and SCEs were very high and chromosome showed structural abnormalities. The sizes of micronuclei formed by vinblastine were larger than those induced by cytosine arabinoside. These results suggest that cytosine arabinoside and vinblastine have highy mutagenic and severe cytotoxic effects on the cultured mouse fibroblasts.

• Environmental Analysis Health and Toxicology
• /
• v.11 no.3_4
• /
• pp.65-68
• /
• 1996

Selaginellae Herba has been used as folk medicine for antineoplastics, coagulants, antidotes and invigorants. To find an in vitro screening method for liver protective effect of Selaginellae Herba in benzo(a)pyrene intoxicated injury were examined in primary cultured rat hepatocytes. Using MTT assay, herba concentration showed dose dependently viability. The lowest concentration of benzo(a)pyrene giving cytotoxicity revealed around 50gM. The hepatoprotective effect of Selaginellae Herba in both water and chloroform extracts was also increased dose dependently.

• Korean Journal of Plant Resources
• /
• v.11 no.2
• /
• pp.168-172
• /
• 1998

The morphlogical characteristics and cytotoxic acitivity for14 local varieties fo Codonopsis lanceolata collected from Korea were examined to get basic data for breeding and selection of good cultivars. Plant height ranged from 197.2 cm to 253.2 com, that from Beakunsan(No.11) was the highest and and that form Samcheok(No.5) was the lowest. Generally, plant height of those form northern area of Korea tends to be shorter than those from Southern area. Leaf width and length of that form Husando (No. 12) was the largest While that form chejudo(No.4) was the smallest and those from other areas showed no significant difference. As to the number of branches, that form Yanggu(No.1) was theleast and those from other areas did not show Chejudo(No.14) were the heaviest. Among 14 varieties tested, Heongseong(No.3) and Huksando(No.6) exhibited strong cytotoxicity , which showed 45.0 an d62.0$\mu\textrm{g}$. $m\ell$ respectively.

• Archives of Pharmacal Research
• /
• v.24 no.4
• /
• pp.300-306
• /
• 2001

A novel and two known bioactive mono-tetrahydrofuran (THF) annonaceous acetogenins, annomocherin (1), annonacin (2) and annomontacin (3), have been isolated from the fractionated ethanolic extracts of the seeds of Annona cherimolia, guided by the brine shrimp lethality test (BST). Their structures were elucidated on the basis of spectroscopic and chemical methods. All compounds have a relative stereochemistry of threo/trans/threo for the mono-THF ring with two flanking hydroxyls. Compound 1 has a double bond at C-23/ 24 of aliphatic chain. Compound 1 was isolated from natural sources for the first time, and was named annomocherin. Two known Compounds 2 and 3 which have never been isolated from this species before, were obtained. Compound 1 exhibited potent and selective cytotoxicities against the breast carcinoma (MCF-7) and kidney carcinoma (A-498) cell lines with 100 to 1,000 times the potency of adriamycin. In brine shrimp lethality test (BST), 1-3 exhibited cytotoxicity.

• Journal of the Korean institute of surface engineering
• /
• v.39 no.1
• /
• pp.43-48
• /
• 2006

The oxidation behavior and Biocompatibility of pure Ti and Ti-8Ta-3Nb alloy were studied in dry air atmosphere. The specimens showed that Ti-8Ta-3Nb alloy had higher oxidation resistance than pure Ti at $650^{\circ}C$. Cytotoxicity test also revealed that moderate oxidation treatment lower cell toxicity and Ti-8Ta-3Nb alloy showed better results compared with pure Ti. The weight gains during the oxidation increase rapidly at temperature above $600^{\circ}C$.

• 한국생물공학회:학술대회논문집
• /
• 2001.11a
• /
• pp.151-162
• /
• 2001

Korea mountain ginseng known as oriental miracle drug is an important medicinal plant. The effect of mountain ginseng adventitious roots extract has been described. The valuable root of mountain ginseng contained several kinds of ginsenosides that have been confirmed to have many active functions for the human body. However, the study of mountain ginseng has a limit because the price of wild ginseng is very expensive and rare. The mountain ginseng adventitious roots were derived from mountain ginseng callus that were induced from mountain ginseng roots. Adventitious roots were separated from callus and grown in solid media(Murachige and stoog media). It was cultured in a 20L bioreactor. After culturing for 40days, adventitious roots were harvested. Afterwards the harvested mountain ginseng adventitious roots were dryed and extracted. We examined the effect on melanogenesis of mountain ginseng adventitious roots extrac. Here, we report the inhibitory effect of melanin biosynthesis on the adventitious roots extract of In vitro test. Also, we assessed the safety of adventitious roots extract. In vitro, cytotoxicity of adventitious roots extract was assessed in mouse fibroblast using two method: The neutral red uptake assay and the MTT assay. In vivo, the allergic and irritant were patch tested in 30 patients. Consequently, extract of mountain ginseng adventitious roots have inhibitory effect on melanin biosynnthesis in B-16 melanoma cell test, tyrosinase inhibitory test and DOPA auto-oxidation test. There were decreased 86%(0.5% concentration), 45%(1% concentration) and 61%(1% concentration), respectively

• Bulletin of the Korean Chemical Society
• /
• v.32 no.7
• /
• pp.2397-2404
• /
• 2011

Variable selection k nearest neighbor QSAR modeling approach was applied to a data set of 80 3-arylisoquinolines exhibiting cytotoxicity against human lung tumor cell line (A-549). All compounds were characterized with molecular topology descriptors calculated with the MolconnZ program. Seven compounds were randomly selected from the original dataset and used as an external validation set. The remaining subset of 73 compounds was divided into multiple training (56 to 61 compounds) and test (17 to 12 compounds) sets using a chemical diversity sampling method developed in this group. Highly predictive models characterized by the leave-one out cross-validated $R^2$ ($q^2$) values greater than 0.8 for the training sets and $R^2$ values greater than 0.7 for the test sets have been obtained. The robustness of models was confirmed by the Y-randomization test: all models built using training sets with randomly shuffled activities were characterized by low $q^2{\leq}0.26$ and $R^2{\leq}0.22$ for training and test sets, respectively. Twelve best models (with the highest values of both $q^2$ and $R^2$) predicted the activities of the external validation set of seven compounds with $R^2$ ranging from 0.71 to 0.93.

• Journal of Technologic Dentistry
• /
• v.27 no.1
• /
• pp.19-25
• /
• 2005

Ti6Al4V alloy have been mainly used as implant materials. Ti-6Al-4V alloy instead of pure Ti is being widely used as biomaterials has some characteristics such as high fatigue strength, tensile strength. But it has been reported recently that vanadium component expresses cytotoxicity and carcinogenicity and aluminium component is related with dementia of Alzheimer type. In order to overcome their detrimental effects, $\beta$-phase stabilizer Nb was chosen in the present study, in addition Ta and Hf were added to Ti-40wt.%Nb alloy to improve its mechanical properties. This paper was described the influence of heat treatment of Ti-40Nb alloys with 2wt%Ta, 2wt%Hf on the mechanical properties. Specimens of Ti alloys were melted in vacuum arc furnace and homogenized at 1050$^{\circ}C$ for 24 hr. and then were aged after solution heat treat at $\alpha+\beta$ and $\beta$ regions. The mechanical properties of Ti alloys were analysed by hardness test, tensile test, elongation test and SEM test. The results can be summarized as follows: 1. The mechanical properties Ti-40wt.%Nb were improved when 2wt.% Ta and 2wt.%Hf were added. 2. The higher tensile strength value and elongation at solution heat treat was higher than solution heat treat and then were aged.

• Korean Journal of Oriental Medicine
• /
• v.15 no.3
• /
• pp.99-105
• /
• 2009

In this research, the genotoxic effects of Pinelliae Rhizoma (PR) extracts, one of famous herbal agents in Korean medicine were evaluated using the mouse micronucleus test. PR extracts was administered once a day for 2 continuous days by oral gavage to male ICR mice at doses of 2000, 1000, and 500 mg/kg. Cyclophosphamide was used as a known genotoxic agent in a positive control. The appearance of a micronucleus is used as an index for genotoxic potential. No PR extracts treatment-related abnormal clinical signs, body weight changes and mortalities were detected. Significant (p<0.01) increases of the numbers of polychromatic erythrocytes contain micronucleus in prepared bone marrow cells were detected in CPA and PR extracts 2000 mg/kg treated groups as compared with intact control, respectively. The results of intraperitoneal dose mouse bone marrow cell micronucleus test of PR extracts were positive in the present study. It is considered that there were no problems from cytotoxicity of PR extracts tested in this study because the polychromatic erythrocyte ratio was detected as > 0.42 in all tested groups.