• 한국농림기상학회지
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• 제9권2호
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• pp.109-120
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• 2007

한국 산악 경관에서의 물과 탄소의 순환을 이해하고 예측하기 위해서는 물과 탄소의 유역 내 이동 경로, 저류, 체류시간 및 상호작용에 대한 정보가 선행되어야 한다. 이와 관련하여 본 논문에서는 HydroKorea 및 CarboKorea 연구에서 사용하고 있는 연구 방법들과 현재까지의 주요 결과를 소개한다. 유역 내 다양한 수문순환 과정을 이해하기 위해 지하수위 변동, end-member mixing model, 교차상관 분석, 대기 기원의 천연방사성 동위원소를 이용하였다. 광릉 산림 유역에서는 지표유출의 기여도가 상대적으로 높았고, 강수량과 강수강도 및 패턴의 변화가 물의 유출경로와 체류 시간에 영향을 주었다. 특히, 몬순으로 인한 강수형태와 유역 내 수문과정의 변화가 탄소 순환에 영향을 미쳤는데, 지속적인 강우의 유입이 산림토양의 표층에 분포하는 고농도의 용존유기탄소의 유출을 증가시켰다. 본 연구를 통하여 시도된 수문순환과정에 대한 정량적인 규명은 기후 변화가 수자원 관리와 산림유역 탄소순환에 미치는 영향을 예측하기 위한 과학적 방법론을 확립하는데 기여할 것으로 기대된다.

• 한국수소및신에너지학회논문집
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• 제13권3호
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• pp.181-189
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• 2002

A series of multicomponent $Zr_{0.8}Ti_{0.2}Mn_{0.4}V_{0.6}Ni_{1-x}Fe_{x}$ (x=0.00, 0.08, 0.15, 0.22, and 0.30) alloys are prepared and their oystal structure and P-C-T curves are examined. The electrochemical properties of these allqys such as activation conditions, discharge capacity, cycling performance are also investigated. $Zr_{0.8}Ti_{0.2}Mn_{0.4}V_{0.6}Ni_{1-x}Fe_{x}$ (x=0.00, 0.08, 0.15, 0.22 and 0.30) have the C14 Laves phase hexagonal structure. The electrode was activated by the hot-charging treatment. The best activation conditions were the current density 120 mA/g and the hot-charging time 12h at $80^{\circ}C$ in the case of the alloy with x=0.00. The discharge capacity increased rapidly until the fourth cycle and then decreased. The discharge capacity increased again from the 13th cycle, arriving at 234 mAh/g at the 50th cycle. The discharge capacily just after activation decreases with the increase in the amount of the substituted Fe but the cycling performance is improved. The discharge capacity after activation of the alloy with x=0.00 is 157 mAh/g at the current density 120 mA/g. $Zr_{0.8}Ti_{0.2}Mn_{0.4}V_{0.6}Ni_{0.85}Fe_{0.15}$ is a good composition with a medium quantity of discharge capacities and a good cycling performance. The ICP analysis of the electrolyte for these electrodes after 50 charge-discharge cycles shows that the concentrations of V and Zr are relatively high. Another series of multicomponent $Zr_{0.8}Ti_{0.2}Mn_{0.4}V_{0.6}Ni_{0.85}M_{0.15}$ (M = Fe, Co, Cu, Mo and Al) alloys are prepared. They also have the C14 Laves phase hexagonal structure. The alloys with M = Co and Fe have relatively larger hydrogen storage capacities. The discharge capacities just after activation are relatively large in the case of the alloys with M = Al and Cu. They are 212 and 170 mAh/g, respectivety, at the current density 120mA/g. The $Zr_{0.8}Ti_{0.2}Mn_{0.4}V_{0.6}Ni_{0.85}Co_{0.15}$ alloy is the best one with a relatively large discharge capacity and a good cycling performance.

• 한국수소및신에너지학회논문집
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• 제34권2호
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• pp.178-186
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• 2023

Three activation methods (constant voltage, current cycling, and hydrogen pumping) were applied to investigate the effects on the performance of the membrane electrode assembly (MEA) loaded with PtCo/C catalyst. The current cycling protocol took the shortest time to activate the MEA, while the performance after activation was the worst among the all activation methods. The constant voltage method took a moderate activation time and exhibited the best performance after activation. The hydrogen pumping protocol took the longest time to activate the MEA with moderate performance after activation. According to the distribution of relaxation time analysis, the improved performance after the activation mainly comes from the decrease of charge transfer resistance rather than the ionic resistance in the cathode catalyst layer, which suggests that the existence of water on the electrode is the key factor for activation.

• International Journal of Fuzzy Logic and Intelligent Systems
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• 제5권4호
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• pp.360-366
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• 2005

In the past, when there were few vehicles on the road, the T.O.D.(Time of Day) traffic signal worked very well. The T.O.D. signal operates on a preset signal cycling which cycles on the basis of the average number of average passenger cars in the memory device of an electric signal unit. Now days, with increasing many vehicles on restricted roads, the conventional traffic light creates startup-delay time and end-lag-time. The conventional traffic light loses the function of optimal cycle. And so, $30-45\%$ of conventional traffic cycle is not matched to the present traffic cycle. In this paper we proposes electro sensitive traffic light using fuzzy look up table method which will reduce the average vehicle waiting time and improve average vehicle speed. Computer simulation results prove that reducing the average vehicle waiting time which proposed considering passing vehicle length for optimal traffic cycle is better than fixed signal method which doesn't consider vehicle length.

• International Journal of Fuzzy Logic and Intelligent Systems
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• 제3권1호
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• pp.66-71
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• 2003

In the past, when there were few vehicles on the road, the T.O.D.(Time of Day) traffic signal worked very well. The T.O.D. signal operates on a preset signal cycling which cycles on the basis of the average number of average passenger cars in the memory device of an electric signal unit. Today, with increasing traffic and congested roads, the conventional traffic light creates startup-delay time and end lag time so that thirty to forty-five percent efficiency in traffic handling is lost, as well as adding to fuel costs. To solve this problem, this paper proposes a new concept of optimal green time algorithm, which reduces average vehicle waiting time while improving average vehicle speed using fuzzy rules and neural networks. Through computer simulation, this method has been proven to be much more efficient than fixed time interval signals. Fuzzy Neural Network will consistanly improve average waiting time, vehicle speed, and fuel consumption.

• 대한치과보철학회지
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• 제38권3호
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• pp.336-347
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• 2000

Statement of problem. Luster loss in esthetic anterior ceromer restoration can occur and can be related with rough surface texture. Understanding durability of surface finishing methods like polishing and surface coating have critical importance. Purpose. This study evaluated the effect of tooth brushing and thermal cycling on surface luster of 3 ceromer systems (Artglass, Targis, Sculpture) treated with different surface finishing methods. Material and methods. Seventy-two samples were prepared: 12 for control group Z100, 12 for Artglass, 24 for Targis, and 24 for Sculpture. Half of the Targis and Sculpture were polished according to the manufacturer's recommendation. The rest of the samples were coated with staining and glazing solution for Targis and Sculpture, respectively. All specimens were subjected to 10,000 cycles between $5^{\circ}C\;and\;55^{\circ}C$ with 30 seconds dwell time. Tooth brushing abrasion tests were performed in a customized tooth brushing machine with 500g back and forth for 20,000 cycle. Luster comparisons were based on grading after direct observation, and light reflection area was measured with Image analysis software. Results. All materials showed an decrease in luster grade after thermal cycling and tooth brushing. The post-tooth brushing results revealed that the glazed Sculpture had greater mean luster grade than did any other groups. While, the stained Targis group showed greatest changes after tooth brushing (p < 0.05), polished Targis and Sculpture did not show significant changes. However, glazed Sculpture showed discretely fallen out glaze resin. Conclusion. From the results of this study, all of the ceromer specimens were much glossy than control composite group after tooth brushing. coatings used for Targis and Sculpture had not durability for long term use.

• Bulletin of the Korean Chemical Society
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• 제32권2호
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• pp.599-604
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• 2011

In typical proteomic analysis, trypsin digestion is one of the most time-consuming steps. Conventional proteomic sample preparation methods use an overnight trypsin digestion method. In this study, we compared high-pressure cycling technology (PCT) during enzyme digestion for proteome analysis to the conventional method. We examined the effect of PCT on enzyme activity at temperatures of 25, 37, and $50^{\circ}C$. Although a fast digestion (1 h) was used for the standard protein mixture analysis, the PCT-assisted method with urea showed better results for protein sequence coverage and the number of peptides identified compared with the conventional method. There was no significant difference between temperatures for PCT-assisted digestion; however, we selected PCT-assisted digestion with urea at $25^{\circ}C$ as an optimized method for fast enzyme digestion, based on peptide carbamylation at these conditions. The optimized method was used for stem cell proteome analysis. We identified 233, 264 and 137 proteins using the conventional method with urea at $37^{\circ}C$ for 16h, the PCT-assisted digestion with urea at $25^{\circ}C$ for 1 h, and the non-PCT-assisted digestion with urea at $25^{\circ}C$ for 1 h, respectively. A comparison of these results suggests that PCT enhanced the enzyme digestion by permitting better access to cleavage sites on the proteins.

• International Journal of Advanced Culture Technology
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• 제7권1호
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• pp.231-236
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• 2019

Background/Objectives: This paper proposes a dual mode feedback-controlled cycling system for children with spastic cerebral palsy to rehabilitate upper extremities. Repetitive upper limb exercise in this therapy aims to both reduce and analyze the abnormal torque patterns of arm movements in three- dimensional space. Methods/Statistical analysis: We designed an exercycle robot which consists of a BLDC motor, a torque sensor, a bevel gear and bearings. Mechanical structures are customized for children of age between 7~13 years old and induces reaching and pulling task in a symmetric circulation. The shafts and external frames were designed and printed using 3D printer. While the child performs active/passive exercise, angular position, angular velocity, and relative torque of the pedal shaft are measured and displayed in real time. Findings: Experiment was designed to observe the features of a cerebral palsy child's exercise. Two children with bilateral spastic cerebral palsy participated in the experiment and conducted an active exercise at normal speed for 3 sets, 15 seconds for each. As the pedal reached 90 degrees and 270 degrees, the subject showed minimum torque, in which the child showed difficulty in the pulling task of the cycle. The passive exercise assisted the child to maintain a relatively constant torque while visually observing the movement patterns. Using two types of exercise enabled the child to overcome the abnormal torque measured in the active data by performing the passive exercise. Thus, this system has advantage not only in allowing the child to perform the difficult task, which may contribute in improving the muscle strength and endurance and reducing the spasticity but also provide customizable system according to the child's motion characteristic. Improvements/Applications: Further study is needed to observe how passive exercise influences the movement characteristics of an active motion and how customized experiment settings can optimize the effect of pediatric rehabilitation for spastic cerebral palsy.

• Journal of Microbiology and Biotechnology
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• 제31권10호
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• pp.1430-1437
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• 2021

Cronobacter sakazakii is an opportunistic pathogenic bacterium found in powdered infant formula and is fatal to neonates. Antibiotic resistance has emerged owing to overuse of antibiotics. Therefore, demand for high-yield bacteriophages as an alternative to antibiotics has increased. Accordingly, we developed a modified mass-production method for bacteriophages by introducing a two-stage self-cycling (TSSC) process, which yielded high-concentration bacteriophage solutions by replenishing the nutritional medium at the beginning of each process, without additional challenge. pH of the culture medium was monitored in real-time during C. sakazakii growth and bacteriophage CS01 propagation, and the changes in various parameters were assessed. The pH of the culture medium dropped to 5.8 when the host bacteria reached the early log phase (OD₅₄₀ = 0.3). After challenge, it decreased to 4.65 and then recovered to 4.94; therefore, we set the optimum pH to challenge the phage at 5.8 and that to harvest the phage at 4.94. We then compared phage production during the TSSC process in jar-type bioreactors and the batch culture process in shaker flasks. In the same volume of LB medium, the concentration of the phage titer solution obtained with the TSSC process was 24 times higher than that obtained with the batch culture process. Moreover, we stably obtained high concentrations of bacteriophage solutions for three cycles with the TSSC process. Overall, this modified TSSC process could simplify large-scale production of bacteriophage CS01 and reduce the unit cost of phage titer solution. These results could contribute to curing infants infected with antibiotic-resistant C. sakazakii.

• Asian-Australasian Journal of Animal Sciences
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• 제22권6호
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• pp.774-780
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• 2009

The objectives of this study were to test the efficacy of induction of estrus and determine the timing of ovulation in relation to preovulatory LH and estrogen surges in cycling Murrah buffaloes subjected to Heatsynch protocol (GnRH-$PGF_2{\alpha}$-Estradiol benzoate). In experiment 1, the buffaloes (n = 10) were treated with Heatsynch protocol and observed for estrus and ovulation. In experiment 2 and 3, 30 cycling Murrah buffaloes were used to investigate the efficacy of Heatsynch protocol in terms of conception rates in summer (experiment 2) and winter (experiment 3) seasons. Fixed time A.I. was performed in all the buffaloes at 48 and 60 h post-estradiol benzoate (EB) injection. All buffaloes responded to the Heatsynch protocol with expression of estrus for which ovulations were induced in 8 buffaloes (80%). Mean time interval from the EB injection to ovulation was 50.0${\pm}$2.0 h (range 44.0 to 60.0 h). The interval from the end of LH surge to ovulation was 18.5${\pm}$2.47 h (range 8 to 26 h). The interval from end of estrogen surge to ovulation was 26.75 ${\pm}$2.07 h (range 22 to 36 h). Mean LH peak after EB injection occurred at 20.81${\pm}$1.61 h (range 14 to 28 h) and mean estrogen peak after EB injection occurred at 9.62${\pm}$1.03 h (range 7 to 16 h). Hence, the mean estrogen peak preceded the mean LH peak by 11 h. It was observed that the percentage of conceptions to total number of estruses for control buffaloes was 18 and 30 in summer and winter, respectively, whereas it increased to 26 and 40 in Heatsynch treated buffaloes in respective seasons. The results suggest the possibility of using Heatsynch treatment followed by fixed time A.I. in buffaloes for fertility improvement, especially since the incidence of silent heat in buffaloes is very high.