• Journal of Microbiology and Biotechnology
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• v.15 no.6
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• pp.1392-1396
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• 2005

Proteolytic digestion and CD measurement of wild-type and mutant cyclic AMP receptor proteins (CRPs) were performed either in the presence or absence of cyclic nucleotide. Results indicated that transition of a structural change to the hinge region by the binding of cAMP to the anti site was required for the binding of cAMP to the syn site near the hinge region and, although the occupancy of cAMP in the anti site increased the protein stability, CRP adopted more a stable conformation by the binding of cAMP to the syn site.

• Journal of the Korean Ceramic Society
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• v.29 no.10
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• pp.806-814
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• 1992

The aim of this study was to investigate the change in compressive strength, freacture behavior and degradation of piezoelectric properties with compressive cyclic loading in Pb(Zr, Ti)O3 of tetragonal, morphotropic phase boundary and rhombohedral composition. The highest compressive strength was found in rhombohedral composition. After poling treatment the strength increased by 8.4% and 6.5% in tetragonal and morphotropic phase boundary compositions respectively while changed little in rhombohedral. The increase of compressive strength after poling treatment is believed to be due to the internal stress around grain boundary by domain alginment toward electric field direction in the microstructures having tetragonality and the occurrence of domain switching to the direction perpendicular to electrical field during fracture. Fracture mode relatively change from transgranular to intergranular was observed in the large grain sized tetragonal and morphotropic phase boundary compositions before and after poling but the transgranular fracture mode always remained in the rhombohedral composition. From the X-ray diffractometer analysis the domains parallel to the electric field direction is known to undergo rearrangement during the cyclic loading into random direction that is responsible for the degradation of piezoelectric property.

• The Korean Journal of Zoology
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• v.33 no.4
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• pp.446-453
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• 1990

In order to investigate the retinoic acid indticed-differentiation of F9 teratocarcinoma stem cell, we have analyzed the change of cell morphology and laminin expression after exposure to retinoic add and cyclic AMP. It is shown that undifferentiated F9 stem cells grow as closely packed colonies, and it is difficult to distinguish cell-cell boundaries. After retinoic add and dibutyryl cyclic AMP treatment, F9 cells assume a flat morphology characterized by perinuclear granules and arrest growth. According to Northern blot analysis, laminin expression was increased markedly after retinoic acid treatment. Laminin Bi gene expression was increased at least 30-fold and laminin B2 gene expression was increased approximately 20-fold during differentiation process. Employing immunofluoresence analysis, it was proved that the synthesis of laminin protein was low level in F9 stem cell whereas it became high level in retinoic acid treated F9 cell and the laminin protein was largely accumulated in the cell surface. Our results suggest that induction of laminin Bi and B2 genes m F9 cells is retinoic acid-mediated control, and morphological change and differentiation of F9 cells might be associated with laminin gene expression.

• Journal of the Korean Geosynthetics Society
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• v.10 no.1
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• pp.19-28
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• 2011

This paper presents the results of an investigation into the effect of cyclic freezing-thawing on the compressive strength characteristics of decomposed granite soils. A plane strain compression (PSC) tests were performed on a series of test specimens with different freezing-thawing cycles and fine contents to investigate the change in compressive strength under the process of freezing-thawing cycles. Also performed were scanning electron microscope (SEM) tests to investigate the change in structural rearrangement from a micro-scale point of view. The test results showed that the soil particles tend to conglomerate when subject to cycles of freezing and thawing, and that the soil with less fines exhibited decreased shear strength due to the cyclic freezing-thawing while the soils with a larger fine content showed the opposite trend.

• Geomechanics and Engineering
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• v.24 no.6
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• pp.519-529
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• 2021

In coal mining activities, the abutment stress of the coal has to undergo cyclic loading and unloading, affecting the strength and seepage characteristics of coal; additionally, it can cause dynamic disasters, posing a major challenge for the safety of coal mine production. To improve the understanding of the dynamic disaster mechanism of gas outburst and rock burst coupling, triaxial devices are applied to axial pressure cyclic loading-unloading tests under different axial stress peaks and different pore pressures. The existing empirical formula is use to perform a non-linear regression fitting on the relationship between stress and permeability, and the damage rate of permeability is introduced to analyze the change in permeability. The results show that the permeability curve obtained had "memory", and the peak stress was lower than the conventional loading path. The permeability curve and the volume strain curve show a clear symmetrical relationship, being the former in the form of a negative power function. Owing to the influence of irreversible deformation, the permeability difference and the damage of permeability mainly occur in the initial stage of loading-unloading, and both decrease as the number of cycles of loading-unloading increase. At the end of the first cycle and the second cycle, the permeability decreased in the range of 5.777 - 8.421 % and 4.311-8.713 %, respectively. The permeability decreases with an increase in the axial stress peak, and the damage rate shows the opposite trend. Under the same conditions, the permeability of methane is always lower than that of helium, and it shows a V-shape change trend with increasing methane pressures, and the permeability of the specimen was 3 MPa > 1 MPa > 2 MPa.

• Proceedings of the Korea Concrete Institute Conference
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• 2002.10a
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• pp.99-104
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• 2002

This paper presents an analytical prediction of the fatigue damage of reinforced concrete bridge columns subjected to cyclic load. Material nonlinearity is taken into account by comprising tensile, compressive and shear models of cracked concrete and a model of reinforcing steel. The smeared crack approach is incorporated. In boundary plane at which each member with different thickness is connected, local discontinuity in deformation due to the abrupt change in their stiffness can be taken into account by introducing interface element. The effect of number of load reversals with the same displacement amplitude has been also taken into account to model the reinforcing steel and concrete. The proposed numerical method for fatigue damage of reinforced concrete bridge columns subjected to cyclic load is verified by comparison with reliable experimental results.

• Corrosion Science and Technology
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• v.10 no.3
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• pp.95-100
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• 2011

Protective coatings play an important role in the protection of metallic structures against corrosive environment. The main function of anticorrosive coating is to prevent the materials from corrosive agents, such as water, oxygen and ions. In the study, the corrosion protection properties of urethane and epoxy coating systems were evaluated using EIS methods exposed to the corrosion acceleration test such as Norsok M501, Prohesion and hygrothermal cyclic test. AFM analysis of the coating systems was carried out to monitor the change of roughness of coatings. Urethane coating system was more stable than the epoxy coating under given cyclic conditions. Water uptake into the urethane coatings was less than that into the epoxy coating. The urethane coating system showed better corrosion protection than epoxy coating system based on the changes of the impedance modulus at low frequency region with exposure time. Consequently, the corrosion protection properties of the epoxy and urethane coatings was well correspond with their surface roughness changes and water uptakes.

• Journal of Ocean Engineering and Technology
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• v.8 no.1
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• pp.154-164
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• 1994

In this paper, fatigue testing was carried out under the change of aging time(0.5,1.5.10hrs) by electric heating method which is one of the useful method in the application of Robot's actuator. Fatigue degradation behaviors such as cyclic deformation property, amount of deformation, decrease in recovery and variation of transformation temperature for each specimen were examined closely, and the effect of aging time condition was studied on their fatigue degradation behaviors.

• Structural Engineering and Mechanics
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• v.55 no.5
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• pp.913-929
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• 2015

The ratcheting and strain cyclic behaviour of joined conical-cylindrical shells under uniaxial strain controlled, uniaxial and multiaxial stress controlled cyclic loading are investigated in the paper. The elasto-plastic deformation of the structure is simulated using Chaboche non-linear kinematic hardening model in finite element package ANSYS 13.0. The stress-strain response near the joint of conical and cylindrical shell portions is discussed in detail. The effects of strain amplitude, mean stress, stress amplitude and temperature on ratcheting are investigated. Under strain symmetric cycling, the stress amplitude increases with the increase in imposed strain amplitude. Under imposed uniaxial/multiaxial stress cycling, ratcheting strain increases with the increasing mean/amplitude values of stress and temperature. The abrupt change in geometry at the joint results in local plastic deformation inducing large strain variations in the vicinity of the joint. The forcing frequency corresponding to peak axial ratcheting strain amplitude is significantly smaller than the frequency of first linear elastic axial vibration mode. The strains predicted from quasi static analysis are significantly smaller as compared to the peak strains from dynamic analysis.

• Journal of the Korean Society for Heat Treatment
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• v.3 no.2
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• pp.37-44
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• 1990

The effect of the microstructural change on the near threshold fatigue crack growth rate in SM40C steel has been studied using the ${\Delta}K$ decreasing method. Below the total strain amplitude of 0.56%, cyclic softening occured, whereas above this value cyclic hardening occurred in the pearlitic lamellar structure. However, in the spherodized structure the cyclic hardening solely occurred. The crack growth rate in the near-threshold region was decreased with increasing prior austenite grain size and this was due to surface roughness. The crack growth rate of the spherodized structure was lower than that of the pearlite lamellar structure and the ${\Delta}K_{th}$ of the former was higher than that of the latter. It was understood that the crack propagates preferentially through the ferrite phase. The intergranular facets in the near-threshold region appeared in the spherodized structure.