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Stability and Structural Change of cAMP Receptor Protein at Low and High cAMP Concentrations  

GANG JONGBACK (Department of Chemistry)
CHUNG HYE-JIN (Department of Chemistry)
PARK GWI-GUN (Department of Food and Bioengineering, Kyungwon University)
PARK YOUNG-SEO (Department of Food and Bioengineering, Kyungwon University)
CHOI SEONG-JUN (Physiology Laboratory, College of Medicine, Pochon CHA University)
Publication Information
Journal of Microbiology and Biotechnology / v.15, no.6, 2005 , pp. 1392-1396 More about this Journal
Abstract
Proteolytic digestion and CD measurement of wild-type and mutant cyclic AMP receptor proteins (CRPs) were performed either in the presence or absence of cyclic nucleotide. Results indicated that transition of a structural change to the hinge region by the binding of cAMP to the anti site was required for the binding of cAMP to the syn site near the hinge region and, although the occupancy of cAMP in the anti site increased the protein stability, CRP adopted more a stable conformation by the binding of cAMP to the syn site.
Keywords
cAMP receptor protein; protein stability; melting temperature;
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