• Title/Summary/Keyword: Cyclic Bending

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Tethered DNA shear dynamics in the flow gradient plane: application to double tethering

  • Lueth, Christopher A.;Shaqfeh, Eric S.G.
    • Korea-Australia Rheology Journal
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    • v.19 no.3
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    • pp.141-146
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    • 2007
  • We examine the wall contact of a $3\;{\mu}m$ tethered DNA chain's free end under shear with a focus on developing schemes for double-tethering in the application of making scaffolds for molecular wires. At this scale our results are found to be highly dependent on small length scale rigidity. Chain-end-wall contact frequency, mean fractional extension deficit upon contact, and standard deviation in extension upon contact are examined for scaling with dimensionless flow strength, Wi. Predictions made using a one dimensional approximation to the Smoluchowski equation for a dumbbell and three dimensional dumbbell simulations produce extension deficit, standard deviation, and frequency scaling exponents of -1/3, -1/3, and 2/3, respectively whereas more fine-grained Kratky-Porod (KP) simulations produce scaling exponents of -0.48, -0.42, and 0.76. The contact frequency scaling of 2/3 is derived from the known results regarding cyclic dynamics Analytical scaling predictions are in agreement with those previously proposed for ${\lambda}-DNA$. [Ladoux and Doyle, 2000, Doyle et al., 2000]. Our results suggest that the differences between the dumbbell and the KP model are associated with the addition of chain discretization and the correct bending potential in the latter. These scaling results will aide future exploration in double tethering of DNA to a surface.

A Study on Evaluation of Plastic Strain at Notch Tip of Weld HAZ in Steel (강 용접 열영향부 놋치 선단 의 소성 스트레인 평가에 관한 연구)

  • 김태영;임재규;정세희
    • Journal of Welding and Joining
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    • v.2 no.1
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    • pp.41-48
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    • 1984
  • Recrystallization technique was applied to analyze plastic strain at the notch tip of coarse grain HAZ in mild steel (SB 41) and high strength steel (SA 588). The notch tip of specimen was deformed by three point bending. Accumulated displacement (Crack Opening Displacement ${delta}t$) by the monotonic and cyclic loading under room temperature and hot strain embrittlement temperature ($250^{\circ}C$) was 0~1.0mm. Recrystallization heat treatment conditions were $650^{circ}C{ imes}3hr$ for SB 41 and $700^{circ}C{ imes}3hr$ for SA 588. The experimental results obtained were as follows ; 1) Distribution of the effective plastic strain at plastic zone was appeared by the function of crack opening displacement, and plastic zone or the effective plastic strain increased with crack opening displacement. 2) Plastic strain at notch tip of HAZ due to accumulated hot strain calculated as follows. .epsilon. over bar $_{p}$ = .epsilon. over bar $_{cr}$ (x/ $R_{x}$ ) $^{m}$ (m=0.25) 3) Work hardending ratio of notch tip for hot strain was linearly increased with .epsilon. over bar $_{max}$ and dependent upon the material types.s.

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Strain distribution between CFRP strip and concrete at strengthened RC beam against shear

  • Anil, Ozgur;Bulut, Nalan;Ayhan, Murat
    • Structural Engineering and Mechanics
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    • v.41 no.4
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    • pp.509-525
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    • 2012
  • In recent years, CFRP material usage in strengthening applications gradually became widespread. Especially, the studies on the strengthening of shear deficient reinforced concrete beams with CFRP strips are chosen as a subject to numerous experimental studies and research on this subject are increased rapidly. The most important variable, that is affected on the failure mode of CFRP strips and that is needed for determining the shear capacity of the strengthened reinforced concrete beams, is the strain distribution between CFRP strips and concrete. Numerous experimental studies are encountered in the literature about the determination of strain distribution between CFRP strips and concrete. However, these studies mainly focused on the CFRP strips under axial tension. There are very limited numbers of experimental and analytic studies examining the strain distribution between concrete and CFRP strips, which are under combined stresses due to the effects of shear force and bending moment. For this reason, existing experimental study in the literature is used as model for ANSYS finite element software. Nonlinear finite element analysis of RC beams strengthened against shear with CFRP strips under reverse cyclic loading is performed. The strain distributions between CFRP strips and concrete that is obtained from finite element analysis are compared with the results of experimental measurements. It is seen that the experimental results are consisted with the results derived from the finite element analysis and important findings on the strain distribution profile are reached by obtaining strain values of many points using finite element method.

A Study on the Plasma Nitriding Application for the Durability Improvement of the Exhaust Decoupler (배기계 디커플러의 내구 향상을 위한 플라즈마 질화에 관한 연구)

  • Hur, Deog-Jae;Kim, Sang-Sik;Chung, Tae-Jin;Kim, Do-Hoon
    • Transactions of the Korean Society of Automotive Engineers
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    • v.14 no.3
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    • pp.157-163
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    • 2006
  • This paper described the process of improving durability performance of the exhaust decoupler by the plasma nitriding. The properties of plasma nitriding treatment of AIS1304 stainless steel were tested using specimens before applying plasma nitriding to a mesh ring. In order to analyses the effect of plasma nitriding treatment on the mechanical properties, SEM(Scanning Electron Microscopes), roughness and hardness tester were used. Based on specimen plasma nitriding, we could find appropriate condition for application to the mesh ring of decoupler. To confirm the improved durability performance, we compared the number of cycles, which reaches to fracture, of the nitrided decoupler and that of the unnitrided decoupler by the bending cyclic test. In this test, the durability and wear resistance of the mesh ring are significantly improved by plasma nitriding treatment.

Experimental and numerical analysis of fatigue behaviour for tubular K-joints

  • Shao, Yong-Bo;Cao, Zhen-Bin
    • Structural Engineering and Mechanics
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    • v.19 no.6
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    • pp.639-652
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    • 2005
  • In this paper, a full-scale K-joint specimen was tested to failure under cyclic combined axial and in-plane bending loads. In the fatigue test, the crack developments were monitored step by step using the alternating current potential drop (ACPD) technique. Using Paris' law, stress intensity factor, which is a fracture parameter to be frequently used by many designers to predict the integrity and residual life of tubular joints, can be obtained from experimental test results of the crack growth rate. Furthermore, a scheme of automatic mesh generation for a cracked K-joint is introduced, and numerical analysis of stress intensity factor for the K-joint specimen has then been carried out. In the finite element analysis, J-integral method is used to estimate the stress intensity factors along the crack front. The numerical stress intensity factor results have been validated through comparing them with the experimental results. The comparison shows that the proposed numerical model can produce reasonably accurate stress intensity factor values. The effects of different crack shapes on the stress intensity factors have also been investigated, and it has been found that semi-ellipse is suitable and accurate to be adopted in numerical analysis for the stress intensity factor. Therefore, the proposed model in this paper is reliable to be used for estimating the stress intensity factor values of cracked tubular K-joints for design purposes.

Seismic experiment and analysis of rectangular bottom strengthened steel-concrete composite columns

  • Hui, Cun;Zhu, Yanzhi;Cao, Wanlin;Wang, Yuanqing
    • Steel and Composite Structures
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    • v.20 no.3
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    • pp.599-621
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    • 2016
  • In order to study the working mechanism of rectangular steel-concrete composite columns subjected to compression-bending load and further determine the seismic performance index, a bottom strengthened rectangular steel reinforced concrete (SRC) column with concealed steel plates and a bottom strengthened rectangular concrete filled steel tube (CFST) columns were proposed. Six column models with different configurations were tested under horizontal low cyclic loading. Based on the experiments, the load-bearing capacity, stiffness and degradation process, ductility, hysteretic energy dissipation capacity, and failure characteristics of the models were analyzed. The load-bearing capacity calculation formulas for a normal section and an oblique section of bottom strengthened rectangular steel-concrete composite columns were pesented and a finite element (FE) numerical simulation of the classical specimens was performed. The study shows that the load-bearing capacity, ductility, and seismic energy dissipation capacity of the bottom strengthened rectangular steel-concrete composite columns are significantly improved compared to the conventional rectangular steel-concrete composite columns and the results obtained from the calculation and the FE numerical simulation are in good agreement with those from the experiments. The rectangular steel-concrete composite column with bottom strengthened shows better seismic behavior and higher energy dissipation capacity under suitable constructional requirements and it can be applied to the structure design of high-rise buildings.

Binding Aspect of Cyclic AMP Receptor Protein to Symmetrically Synthetic 22-, 28- and 30-Base-Pair lac Promoters

  • Park, Sang-Ho;Lee, Tae-Woo;Hwang, Eun-Suk;Lee, Seung-Ki;Shin, Cha-Gyun;Lee, Bong-Jin
    • Journal of the Korean Magnetic Resonance Society
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    • v.1 no.1
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    • pp.31-44
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    • 1997
  • The effect of the binding of CRP to the symmetrically synthetic 22, 28, and 30 bp lac promoter was investigated by 1H NMR. The binding of cAMP*CRP to the 22 bp DNA did not bring about any changes in the chemical shift values, but did cause selective line broadening of imino proton resonances of specific base pairs. However, The binding of cAMP*CRP to the 28 and 30 bp DNA brought about large changes on the imino proton resonances that seems to be induced by DNA bending. We studied also the role of cAMP as an activator of DNA/CRP complex formation by gel mobility shift assay. Gel mobility shift assay revealed that the cAMP*CRP complex was not able to bind to the 22 bp DNA fragment, but was able to bind to the 28 bp DNA fragment of lac promoter region.

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Experimental study of a pretensioned connection for modular buildings

  • Yu, Yujie;Chen, Zhihua;Chen, Aoyi
    • Steel and Composite Structures
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    • v.31 no.3
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    • pp.217-232
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    • 2019
  • Modular steel buildings consist of prefabricated room-sized structural units that are manufactured offsite and installed onsite. The inter-module connections must fulfill the assembly construction requirements and soundly transfer the external loads. This work proposes an innovative assembled connection suitable for modular buildings with concrete-filled steel tube columns. The connection uses pretensioned strands and plugin bars to vertically connect the adjacent modular columns. The moment-transferring performance of this inter-module connection was studied through monotonic and cyclic loading tests. The results showed that because of the assembly construction, the connected sections were separated under lateral bending, and the prestressed inter-module connection performed as a weak semirigid connection. The moment strength at the early loading stage originated primarily from the contact bonding mechanism with the infilled concrete, and the postyield strength depended mainly on the tensioned strands. The connection displayed a self-centering-like behavior that the induced deformation was reversed during unloading. The energy dissipation originated primarily from frictional slipping of the plugin bars and steel strands. The moment transferring ability was closely related to the section dimension and the arrangements of the plugin bars and steel strands. A simplified strength calculation and evaluation method was also proposed, and the effectiveness was validated with the test data.

Seismic behavior of reinforced concrete column-steel beam joints with and without reinforced concrete slab

  • Tong Li;Jinjie Men;Huan Li;Liquan Xiong
    • Structural Engineering and Mechanics
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    • v.86 no.3
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    • pp.417-430
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    • 2023
  • As the key part in the reinforced concrete column-steel beam (RCS) frame, the beam-column joints are usually subjected the axial force, shear force and bending moment under seismic actions. With the aim to study the seismic behavior of RCS joints with and without RC slab, the quasi-static cyclic tests results, including hysteretic curves, slab crack development, failure mode, strain distributions, etc. were discussed in detail. It is shown that the composite action between steel beam and RC slab can significantly enhance the initial stiffness and loading capacity, but lead to a changing of the failure mode from beam flexural failure to the joint shear failure. Based on the analysis of shear failure mechanism, the calculation formula accounting for the influence of RC slab was proposed to estimate shear strength of RCS joint. In addition, the finite element model (FEM) was developed by ABAQUS and a series of parametric analysis model with RC slab was conducted to investigate the influence of the face plates thickness, slab reinforcement diameter, beam web strength and inner concrete strength on the shear strength of joints. Finally, the proposed formula in this paper is verified by the experiment and FEM parametric analysis results.

Influence of Implant Fixture-Abutment Connection and Abutment Design on Mechanical Strength (임플란트 고정체-지대주 연결부 및 지대주 디자인이 기계적 강도에 미치는 영향)

  • Chun, Mi-Hyun;Jeong, Chang-Mo;Jeon, Young-Chan;Eom, Tae-Gwan;Yoon, Ji-Hoon
    • Journal of Dental Rehabilitation and Applied Science
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    • v.24 no.3
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    • pp.269-281
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    • 2008
  • Fatigue or overload can result in mechanical problems of implant components. The mechanical strength in the implant system is dependent on several factors, such as screw and fixture diameters, material, and design of the fixture-abutment connection and abutment. In these factors, the last rules the strength and stability of the fixture-abutment assembly. There have been some previous reports on the mechanical strength of the fixture-abutment assembly with the compressive bending test or short-term cyclic loading test. However, it is restrictive to predict the long-term stability of the implant system with them. The purpose of this study was to evaluate the influence of the design of the fixture-abutment connection and abutment on the mechanical strength and failure mode by conducting the endurance limit test as well as the compressive bending strength test. Tests were performed according to a specified test(ISO/FDIS 14801) in 4 fixture-abutment assemblies of the Osstem implant system: an external butt joint with Cemented abutment (group BJT), an external butt joint with Safe abutment (group BJS), an internal conical joint with Solid abutment (group CJO), and an internal conical joint with ComOcta abutment (group CJT). The following conclusions were drawn within the limitation of this study. Compressive bending strengths were decreased in order of group BJS(1392.0N), group CJO(1261.8N), group BJT(1153.2N), and group CJT(1110.2N). There were no significant differences in compressive bending strengths between group BJT and group CJT(P>.05). Endurance limits were decreased in order of group CJO(600N), group CJT(453N), group BJS(360N), and group BJT(300N). 3. Compressive bending strengths were influenced by the connection and abutment design of the implant system, however endurance limits were affected more considerably by the connection design.