• 대한기계학회:학술대회논문집
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• 대한기계학회 2001년도 추계학술대회논문집A
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• pp.772-777
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• 2001

The gas-assisted injection molding process is often perceived to be unpredictable, because of the extreme sensitivity of the gas. Since a slight change in design or process parameters can significantly change the resulting gas penetration, few designers and molders have the level of experience with the new gas-assisted injection molding process required for the development of new parts. This paper is concerned with the unified molding for a thick cosmetic chest by using gas-assisted injection molding. CAE analysis was carried out to design the part and the gas channel without inducing sink marks. And based on the part weight measurement, the processing parameters to control gas penetration percentage were chosen through the method of design of experiments. A thick cosmetic chest was successfully produced using the gas assist technology. The sink mark issue associated with the conventional injection molded parts was resolved. Weight savings and cycle-time reduction were also achieved.

• Journal of Microbiology and Biotechnology
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• 제13권6호
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• pp.912-918
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• 2003

Mitotic centromere-associated kinesin (MCAK), which is a novel kinesin with a central motor domain, is believed to playa role in mitotic segregation of chromosome during the M phase of the cell cycle. In the present study, it is shown that a rabbit polyclonal antibody has been produced using the N-terminal region (187 aa) of human MCAK expressed in E. coli as the antigen. To express the N-terminal region in E. coli, the MCAK cDNA fragment encoding N-terminal 187 aa was obtained by PCR and was then inserted into the pET 3d expression vector. Molecular mass of the N-terminal region overexpressed in the presence of IPTG was 23.2 kDa on SDS-PAGE, and the protein was insoluble and mainly localized in the inclusion body that could be easily purified from the other cellular proteins. The N-terminal region was purified by electro-elution from the gel after the inclusion body was resolved on the SDS-PAGE. The antiserum obtained after tertiary immunization with the purified protein specifically recognized HsMCAK when subjected to Western blot analysis, and showed a fluctuation of the protein level during the cell cycle of human Jurkat T cells. Synchronization of the cell-cycle progression required for recovery of cells at a specific stage of the cell cycle was performed by either hydroxyurea or nocadazole, and subsequent release from each blocking at 2, 4, and 7 h. Northern and Western analyses revealed that both mRNA and protein of HsMCAK reached a maximum level in the S phase and declined to a basal level in the G1 phase. These results indicate that a polyclonal antibody raised against the N-terminal region (187 aa) of HsMCAK, overexpressed in E. coli, specifically detects HsMCAK (81 kDa), and it can analyze the differential expression of HsMCAK protein during the cell cycle.

• 한국자동차공학회논문집
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• 제6권2호
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• pp.212-219
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• 1998

Unburned hydrocarbon is a key contributor to both the fuel economy and emissions of automotive engine. Cyclic variation of HC emission is of importance, especially during throttle transients. The real time measurement of hydrocarbon is particularly important to obtain a better understanding of the mechanisms for combustion and emissions, especially during cold start and throttle transient condition. This paper reports the cycle resolved measurement technique of unburned hydrocarbons to quantify rapid changes of in-cylinder concentration in the vicinity of spark plug by using the Fast Response Flame Ionization Detector(FRFID). While this instrument actually measures fuel concentration, its results can be indicative of the AFR behaviour. In order to understand the rapid change of hydrocarbons with cylinder pressure, it is necessary to study the response time delay of the system, including the time associated with gas transportation to FID. And signal from FRFID is correlated with cylinder pressure data to relate changes in mixture preparation to the classic analysis, such as indicated mean effective(IMEF) and ignition delay, etc.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제16권3호
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• pp.1063-1075
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• 2022

In recent years, container techniques have been broadly applied to cloud computing systems to maximize their efficiency, flexibility, and economic feasibility. Concurrently, studies have also been conducted to ensure the security of cloud computing. Among these studies, moving-target defense techniques using the high agility and flexibility of cloud-computing systems are gaining attention. Moving-target defense (MTD) is a technique that prevents various security threats in advance by proactively changing the main attributes of the protected target to confuse the attacker. However, an analysis of existing MTD techniques revealed that, although they are capable of deceiving attackers, MTD techniques have practical limitations when applied to an actual cloud-computing system. These limitations include resource wastage, management complexity caused by additional function implementation and system introduction, and a potential increase in attack complexity. Accordingly, this paper proposes a software-defined MTD system that can flexibly apply and manage existing and future MTD techniques. The proposed software-defined MTD system is designed to correctly define a valid mutation range and cycle for each moving-target technique and monitor system-resource status in a software-defined manner. Consequently, the proposed method can flexibly reflect the requirements of each MTD technique without any additional hardware by using a software-defined approach. Moreover, the increased attack complexity can be resolved by applying multiple MTD techniques.

• Journal of Microbiology and Biotechnology
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• 제33권1호
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• pp.114-122
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• 2023

A family of signal transduction pathways known as wingless type (Wnt) signaling pathways is essential to developmental processes like cell division and proliferation. Mutation in Wnt signaling results in a variety of diseases, including cancers of the breast, colon, and skin, metabolic disease, and neurodegenerative disease; thus, the Wnt signaling pathways have been attractive targets for disease treatment. However, the complicatedness and large involveness of the pathway often hampers pinpointing the specific targets of the metabolic process. In our current study, we investigated the differential metabolic regulation by the overexpression of the Wnt signaling pathway in a timely-resolved manner by applying high-throughput and un-targeted metabolite profiling. We have detected and annotated 321 metabolite peaks from a total of 36 human embryonic kidney (HEK) 293 cells using GC-TOF MS and LC-Orbitrap MS. The un-targeted metabolomic analysis identified the radical reprogramming of a range of central carbon/nitrogen metabolism pathways, including glycolysis, TCA cycle, and glutaminolysis, and fatty acid pathways. The investigation, combined with targeted mRNA profiles, elucidated an explicit understanding of activated fatty acid metabolism (β-oxidation and biosynthesis). The findings proposed detailed mechanistic biochemical dynamics in response to Wnt-driven metabolic changes, which may help design precise therapeutic targets for Wnt-related diseases.

• 한국정보시스템학회지:정보시스템연구
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• 제16권4호
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• pp.131-155
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• 2007

The purpose of this thesis is to study on the philosophical analysis model and its methodological application of information systems research evaluation from critical realist perspective. Fist of all, I examine ontological epistemological methodological assertions of critical realism. Because the philosophy of critical realism is an opportunity for information systems study. I examine Dobson and Mutch's critical realist perspective on actors-structure model. I suggest a critical realist actors-praxis-structure model. This model provides the potential for a new approach to social investigations in its provision of an ontology for the analytical separation of structure and agency. Of most importance might be the incorporation of non-humans into the analysis of social interaction and of technology into the elaboration of structures. I also examine Tsoukas's critical realistic meta-theory of management. I suggest a critical realist IS management model. This model elucidate the nature of management and delineate the scope of applicability of various perspectives on management. The causal powers of management reside in the real domain and, taken together, their logics are contradictory, the effects of their contradictory composition are contingent upon prevailing contingencies. I analyze Carlsson's theory of design knowledge. His framework builds on that the aim of IS design science research is to develop practical knowledge for the design and realization of different classes of IS initiatives, where IS are viewed as socio-technical systems and not just IT artefacts. The framework proposes that the output of IS design science research is practical IS design knowledge in the form of field-tested and grounded technological rules. The IS design knowledge is developed through an IS design science research cycle. In conclusion, I think that IS actors-praxis-structure model, meta-theoretical IS management model, and IS design knowledge model according to critical realistic approach are very useful for IS research evaluation. Nevertheless, important problems are left not resolved.

• 방사성폐기물학회지
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• 제9권4호
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• pp.207-217
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• 2011

UV-Vis 분광광도법과 시간분해 레이저 유도 형광분광법(TRLFS)을 이용하여 흄산의 모사 리간드로 사용한 2,6-Dihydroxybenzoate(DHB)와 U(VI)의 착물형성반응을 조사하였다. U(VI)-DHB 착물 고유의 전하이동 흡수 스펙트럼을 분석한 결과, 착물형성반응은 우라늄-리간드 비가 1:1 또는 1:2 착물을 형성하는 이중 평형반응이며, 산도에 따라 착물종의 분포가 변한다는 것을 밝혔다. 계산된 착물형성상수 (log $K_1$ and log $K_2$)는 $12.4{\pm}0.1$과 $11.4{\pm}0.1$이다. 이에 더하여, TRLFS 방법으로 조사한 결과, DHB는 U(VI) 화학종들의 형광 소광제(quencher)로서 역할을 한다는 것을 확인하였다. 특히, 확인된 U(VI) 화학종 모두(${UO_2}^{2+}$, $(UO_2)_2{(OH)_2}^{2+}$과 $(UO_2)_3(OH)_5{^+})$에서 정적 (static) 및 동적 (dynamic) 소광작용이 공존하는 것으로 관찰되었다. 시간분해 형광 스펙트럼으로부터 리간드 농도에 따른 U(VI) 화학종의 형광세기와 형광수명을 측정하였으며, Stern-Volmer 식을 이용하여 분석하였다. 결정된 정적소광계수(KS)는 ${UO_2}^{2+}$, $(UO_2)_2{(OH)_2}^{2+}$ 과 $(UO_2)_3(OH)_5+$에 대하여 각각 $4.2{\pm}0.1$, $4.3{\pm}0.1$ 과 $4.34{\pm}0.08$이다. Stern-Volmer 식을 이용한 분석 결과, 단일 또는 이중 배위자 구조(mono- and bi-dentate)의 U(VI)-DHB 착물이 모두 정적소광효과에 관여하는 바닥상태 착물임을 확인하였다.

• 자원환경지질
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• 제53권5호
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• pp.553-564
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• 2020

울릉도에서 폭발성 및 분류성 분출 간의 전환은 돔형성 분출을 나타내는 나리분석층과 알봉조면안산암(용암돔)에서 관찰되며, 유동성 마그마, 상승률 및 탈기작용과 같은 요인과 연관된다. 그러나 이들에 대한 해석은 요인들 간의 상호작용이 복잡할 뿐만 아니라 분출과정에서의 거동을 예측하기 어렵기 때문에 지금까지 해결되지 않았다. 이 논문은 나리 칼데라에서 알봉 용암돔 형성과정에서 인지되는 폭발성 및 분류성 분출에 초점을 두었다. 시료는 분석층과 용암돔에서 시간에 따라 채취하였으며, 분출 단계 및 활동 양식과 연계되어 있다. 이 시료들에서 석기 장석미정의 조직은 면적 개수밀도, 평균 미정크기, 결정 종횡비, 석기 결정률 등을 포함하며 정량적으로 분석되었다. 이러한 미정의 조직은 천부에서 분출전 및 분출동시 마그마 과정이 분출 동안 거동변화를 제어하면서 진행되었다는 것을 보여준다. 폭발성 및 분류성 분출 간의 전환은 주로 화도 내 마그마 상승의 역학관계에 의해 일어났으며, 탈기작용과 결정작용이 피드백 메카니즘을 통해 일어남으로서 분류성 및 폭발성 분출의 한 윤회를 초래했다.

• 방사성폐기물학회지
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• 제16권4호
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• pp.397-410
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• 2018

아메리슘(Am)은 사용후핵연료의 장기 방사성 독성에 크게 영향을 주기 때문에 고준위 방사성 폐기물 처분의 장기 안전성 평가에 필수적으로 고려되어야 할 원소이다. 분광학적 방법을 이용한 일부 악티나이드 원소의 화학반응 연구가 활발히 진행되고 있는 반면, 아메리슘에 대한 연구는 아직까지 미비한 상황이다. 이 연구에서는 고순도의 시료를 필요로 하는 화학반응 연구를 위하여 $^{241}Am$ 시료를 정제한 후, 액체섬광계수기와 감마선 및 알파선 스펙트럼을 이용하여 정량과 정성분석을 하였다. 액체 광도파 모세관 셀을 이용한 고감도의 UV-Vis 흡수 분광학과 시간분해 레이저 형광 분광학을 이용하여 Am(III) 가수분해물과 옥살레이트(oxalate, Ox) 착물반응을 조사하였다. 산성조건에서 $Am^{3+}$은 503 nm에서 최대 흡수봉우리를 보이며, 몰흡광계수는 $424{\pm}8cm^{-1}{\cdot}M^{-1}$임을 확인하였다. 중성 이상의 pH 조건에서 형성되는 $Am(OH)_3(s)$ 콜로이드 입자에서는 506-507 nm 파장에서 최대 흡수봉우리가 관측되었다. ${Am(Ox)_3}^{3-}$ 착물은 $Am^{3+}$에 비교하여 흡수 및 발광스펙트럼이 각각 4와 5 nm정도 장파장으로 이동하였고 몰흡광계수와 발광세기도 크게 증가하였다. ${Am(Ox)_3}^{3-}$의 발광수명은 23에서 56ns으로 증가하였고 이는 Am(III)의 내부권에 결합하고 있던 약 여섯 개의 물분자가 옥살레이트의 카르복실기로 치환되었음을 의미한다. 이 결과로부터 ${Am(Ox)_3}^{3-}$은 각 옥살레이트 리간드가 두 자리 결합(bidentate)을 하고 있다는 것을 제안하였다.