• Title/Summary/Keyword: Cyanobacterium

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Draft Genome Database Construction from Four Strains (NIES-298, FCY-26, -27, and -28) of the Cyanobacterium Microcystis aeruginosa

  • Rhee, Jae-Sung;Choi, Beom-Soon;Han, Jeonghoon;Hwang, Soon-Jin;Choi, Ik-Young;Lee, Jae-Seong
    • Journal of Microbiology and Biotechnology
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    • v.22 no.9
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    • pp.1208-1213
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    • 2012
  • Microcystis aeruginosa is a cyanobacterium that can form harmful algal blooms (HABs) producing toxic secondary metabolites. We provide here draft genome information of four strains of this freshwater cyanobacterium that was obtained by the Next Generation Sequencing approach to provide a better understanding of molecular mechanisms at the physiological and ecological levels. After gene assembly, genes of each strain were identified and annotated, and a genome database and G-browser of M. aeruginosa were subsequently constructed. Such genome information resources will enable us to obtain useful information for molecular ecological studies with a better understanding of modulating mechanisms of environmental factors associated with blooming.

Interactive Effects of UV-B and Pesticides on Photosynthesis and Nitrogen Fixation of Anabaena doliolum

  • Chandrai, Lar;Vandana, Pandey
    • Journal of Microbiology and Biotechnology
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    • v.12 no.3
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    • pp.423-430
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    • 2002
  • The effects of UV-B and commercial grade pesticides (butachlor and carbofuran), individually and in combination, were studied on a variety of physiological processes of rice field cyanobacterium Anabaena doliolum. Butachlor was found to be $2-12\%$ more toxic than carbofuran and $4-24\%$ than UV-B on the growth, photosynthesis, lipid peroxidation, membrane permeability, and nitrogenase activity of the test cyanobacterium. Of the three photosynthesis inhibitors, the butachlor-induced inhibition of whole chain was approximately 3 and $21\%$ higher than carbofuran and UV-B, respectively. Although the interaction of the stress factors caused a significant inhibition (P<0.01), it was still less than the additive effect on the parameters investigated, except for PSI.

High Throughput Proteomic Approaches for the Dissection of Light Signal Transduction Pathways in Photosynthetic Cyanobacterium Synechocystis sp.PCC 6803

  • Chung Young-Ho;Park Young Mok
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2002.10a
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    • pp.203-205
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    • 2002
  • Light is an environmental signal that regulates photomovement and main energy source of photosynthesis in the cyanobacterium Synechocystis sp. PCC 6803 (Syn6803). Syn6803 is a popular model system for study of plant functional genomics. In this report, we adopted 2D gel based proteomics study to investigate proteins related with the light absorption and photo-protection in Syn6803. More than 700 proteins were detected on the SDS-gels stained with silver nitrate. Several proteins showing different expression level under various light conditions were identified with MALDI-TOF Mass spectrometry. As a comparison, we also conducted ICAT-based proteome study using WT and cphl (cyanobacterial phytochrome 1) mutant. A cphl deletion led to changes in the expression of proteins involved in translation, photosynthesis including photosystem and CO2 fixation, and cellular regulation. We are currently involved in TAP-tagging method to study protein-protein interactions in search for the molecular component involved in the light signal transduction of Syn6803 photomovement.

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Removal of Microcystis aeruginosa using Pine Needle Extracts (솔잎추출액을 이용한 Microcystis aeruginosa 제거 연구)

  • Choi, Hee-Jeong
    • Journal of Korean Society on Water Environment
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    • v.33 no.1
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    • pp.8-14
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    • 2017
  • Microcystis aeruginosa (M. aeruginosa) is a cyanobacterium species that can form harmful algal blooms in freshwater bodies worldwide. The use of pine needle extract (PNE) to control nuisance algae by allelopathic inhibition will be environmentally friendly and promising. PNE removed successfully upto 98% of M. aeruginosa at the following optimal conditions: pH 7, $25^{\circ}C$ of temperature, 100 rpm of mixing rate, 5 min of mixing time. These results was indicated that the amount of 1 g/L PNE was removed 1g dryweight/L of M. aeruginosa. The kinetic data showed substrate inhibition kinetics and maximum growth rate was obtained when the M. aeruginosa was grown in medium containing 0.5 g/L of initial concentration of PNE. Different substrate inhibition models were fitted to the kinetic data and found the Luong model was best. The model predicted kinetic parameters were in agreement with the experimental findings. The natural extract, PNE, can be a promising inhibition due to its high efficiency and low dose requirements.