• Journal of the Korean Chemical Society
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• v.53 no.6
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• pp.811-813
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• 2009

• Journal of the Korean Chemical Society
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• v.61 no.3
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• pp.122-124
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• 2017

• Journal of the Korean Chemical Society
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• v.59 no.4
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• pp.341-343
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• 2015

• Applied Biological Chemistry
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• v.48 no.3
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• pp.228-232
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• 2005

A yeast strain producing phytase, isolated from a mash of Korean traditional Yakju, was identified as a strain of Saccharomyces cerevisiae and designated as Saccharomyces cerevisiae CY strain. Phytase was produced by CY strain both intracellularly and extracellularly. Total phytase activity by the shaking culture was about two times higher than that of the static culture. The portion of extracellular phytase to total phytase activity ranged between 23 and 49 percent, depending on the glucose concentration in the culture medium. Phytase production was reached at approximately 1 U/ml as total phytase activity and the maximum intracellular phytase activity was 0.17-0.19 U/mg-DCW at late logarithmic growth phase. The optimum reaction pH and temperature of intracellular phytase were 3.5 and $40^{\circ}C$, respectively. Over 95% of the phytate was degraded by growing cells after 36 hours yeast cell culture and about 90% of total phytate was effectively degraded by suspending the whole cell with the biomass of 0.4 mg-DCW/ml-reaction solution after 12 hours degradation reaction.

• Microbiology and Biotechnology Letters
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• v.19 no.3
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• pp.215-221
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• 1991

An autonomous replication sequence (ARS) derived from Cephalosporium acremonium ATCC 20339 was cloned in Sarchuromyces cerevisiae SHY 3 using YIp5 as a cloning vector. A new recombinant plasmid, designated pCY-2, which contained a 3.7 kb BamHI fragment of C. acrenzonium DNA showed the highest stability among the 40 recombinant plasmids composed of the YIp5 2nd ARS of C. ucremoniztm. Also, Southern hybridization and transformation of E, cull with DNA purified from yeast transformants verified that pCY-2 autonomously replicates in yeasts. Transformation efficiency and plasmid stability of pCY-2 in yeast were higher than those ol YRp 7 containing ARS which originated from yeast. Detailed studies by subcloning revealed that two ARSs existed within 2.6 kb of the insert, which is a novel discovery. However, it was concluded that these two ARSs were ligated during the gene manipulation in vitro.

• Asian Journal of Turfgrass Science
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• v.25 no.2
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• pp.147-152
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• 2011

This study was conducted to evaluate the growth characteristics of creeping bentgrass cultivars for fairway of golf course at Yeoju area in Korea. At germination and coverage rate of creeping bentgrass, 'Shark' and 'CY-2' were excellent, 'L-93', 'Alpha', 'T-1 was in order', respectively. Visual qualities of 'Shark' and 'CY-2' also were excellent. Especially, 'Shark', 'T-1', and 'CY-2' showed excellent visual quality in summer days, the critical times for the bentgrass fairway quality. 'Shark', 'CY-2' and 'T-1' were excellent in Chlorophyll contents throughout the evaluation period. Chlorophyll content of 'T-1' was maintained very high in summer. 'CY-2' and 'Shark' showed the best root growth at the beginning of the study and contained longer and hairy roots. Which might make these two cultivars' water absorption easier than other's. NDVI (normalized difference vegetation index)of 'Shark', 'L-93' and 'CY-2' was excellent, respectively. 'T-1' showed the highest density and 'Shark', 'CY-2', 'Alpha', 'L-93' was followed by. The density of 'T-1' was rather increased in summer season, while those of other cultivars were decreased. Three diseases such as anthracnose, brown patch, and dollar spot, were appeared during the evaluation period. 'T-1' and 'L-93' were very sensitive to anthracnose which occurred at the beginning days of the study. The most susceptible cultivars to brown patch were 'Alpha' and 'L-93'. 'T-1' was the least resistant to dollar spot which occurred at the same time with brown patch.

• Journal of Food Hygiene and Safety
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• v.5 no.3
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• pp.111-120
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• 1990

Effects of royal jelly(RJ) on the immune system in normal and cyclophosphamide(CY)-treated mice were investigated. The results were as following: 1. Body weight, spleen weight, thymus weight, WBC, cell-mediated immunity (CMI, contact hypersensitivity to DNFB), humoral immunity (HI, Hemagglutinin-, Hemolysin-titer) were increased or decreased dependent on the day of administration of RJ in normal mice. But it showed no effect on liver weight and RBC. 2. Combined treatment with RJ in CY-treated mice on the day which RJ showed the increasing activities in normal mice inhibited the decrease of survival rate, body weight, spleen weight, WBC and CMI caused by CY, but no effect on the decrease of thymus weight and HI induced by CY.

• Journal of Korean Port Research
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• v.12 no.1
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• pp.35-46
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• 1998

The CY can be said to function in various respect as a buffer zone between the maritime and overland inflow-outflow of container. The amount of storage area needed requires a very critical appraisal at pre-operational stage. A container terminal should be designed to handle and store containers in the most efficient and economic way possible. In order to achieve this aim it is necessary to figure out or forecast numbers and types of containers to be handled, CY area required, and internal handling systems to be adopted. This paper aims to calculate the CY area required for each container handling system in Mokpo New Port. The CY area required are directly dependent on the equipment being used and the storage demand. And also the CY area required depends on the dwell time. Furthermore, containers need to be segregated by destination, weight, class, FCL(full container load), LCL(less than container load), direction of travel, and sometimes by type and often by shipping line or service. Thus the full use of a storage area is not always possible as major unbalances and fluctuations in these flow occuring all the time. The calculating CY area must therefore be taken into account in terms of these operational factors. For solving such problem, all these factors have been applied to estimation of CY area in Mokpo New Port. The CY area required in Mokpo New Port was summarized in the conclusion section.

• Journal of Korea Society of Industrial Information Systems
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• v.15 no.5
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• pp.29-35
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• 2010

In this paper, We implemented discriminating system for various surround illuminants using photo sensor. To discriminate surround illuminants of display device we implemented discriminating system using photo sensor which has output of Ye and Cy. Experimental results shows that we could discriminate surround illuminants by output voltage ratio (Ye/Cy) of photo sensor in the variable luminous intensity of surround illuminants.

• Journal of Microbiology and Biotechnology
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• v.20 no.7
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• pp.1086-1091
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• 2010

This study explored the use of gellan gum as an immobilization matrix for the production of cyclosporin A (CyA) by immobilized spores and mycelia of Tolypocladium inflatum MTCC 557. Different carriers, such as gellan gum, sodium alginate, celite beads, and silica, were tested as immobilization carriers, along with the role of the carrier concentration, biomass weight, number of spore-inoculated beads, and repeated utilization of the immobilized fungus. The maximum CyA production was 274 mg/l when using gellan gum [1% (w/v)], and a mycelial weight of 7.5% (w/v) supported the maximum production of CyA. Additionally, the addition of a combination of $_L$-valine (6 g/l) and $_L$-leucine (5 g/l) after 48 h of fermentation produced 1,338 mg/l of CyA when using gellan gum. The immobilized mycelia beads were found to remain stable for four repetitive cycles, indicating their potential for semicontinuous CyA production.