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Separation and Purification of Angiotensin I-converting Enzyme Inhibitory peptide from Mackerel (고등어 유래 항고혈압 peptide의 분리 정제)

  • DO Jeong-Ryong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.2
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    • pp.153-157
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    • 2000
  • Hydrolysate which inhibit the Angiotensin I-converting enzyme (ACE) was prepared from mackerel muscle by pretense. The ACE inhibitory activity of mackerel muscle hvdrolysate (MMH) was $967 {\mu}g of IC_(50)$. ACE inhibitory peptides were isolated by ultrafiltration, gel permeation column chromatography (GPC), reversed phase column chromatography(RPC), reversed phasehigh performance liquid chromatography (RP-HPLC), and gel permeation high performance liquid chromatography (GP-HPLC) from the MMH. The amino acid sequence of the ACE inhibitory peptides was Tyr-Val-Ala. The $IC_(50)$ of this peptide for ACE from rabbit lung was $1.4 {\mu}M$.

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The case study on interview mise-en-scène of documentary film (다큐멘터리 <노무현입니다> - 인터뷰 미쟝센 사례 연구)

  • Lee, Changjae;Sim, Jihyun
    • Trans-
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    • v.7
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    • pp.81-101
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    • 2019
  • Interviews are so important and meaningful that it is the heart of the documentary. In narrative film, it is focused as the story discourse, whereas documentary is strong in disclosure, which consists of narration and interview. In a TV documentary, interviews use conventional shots repeatedly, whereas in a documentary film that targets a relatively large screen, the interviewer's Mise-en-scene should consider the aesthetic depth. , which released in 2017 and used the audience for the third time in documentary film history, tries to make 40% of the volume of the main volume equal to the same angle. In a TV documentary, it is not uncommon for an interview cut to exceed 30 seconds, and if it exceeds 1 minute, immersion is significantly lowered, so the a mount and compression of the interview disclosure is important for both the director and the audience. In the case of the interview sequence of , it was the key to communicate the sub-plot with a relatively focused with long discourse, which is at least 8 minutes and 30 seconds to a maximum of 11 minutes, in a way that allows the audience to communicate. Furthermore, it was produced after the death of the protagonist Roh Moo Hyun, with the limitation that the voice of the protagonist was excluded, and the voice of Roh Moo Hyun was indirectly reproduced through the surrounding characters. In this study, it covers the meaning and scope of interviews in the production of documentary, and the aesthetic applications of the Mise-en-scene.

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OFDM Communication System Using the Additive Control Tone for PAPR Reduction (PAPR 저감을 위하여 부가 Control 톤을 이용하는 OFDM 통신 시스템)

  • Kim Jin-Kwan;Lee Ill-Jin;Ryu Heung-Gyoon
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.16 no.12 s.103
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    • pp.1229-1238
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    • 2005
  • OFDM(Orthogonal Frequency Division Multiplexing) communications system is very attractive for the high data rate wireless transmission. However, it may be distorted in the nonlinear HPA(High Power Amplifier) since OFDM signal has hish PAPR(Peak-to-Average Power Ratio). In this paper, a new method using control tone is studied for reducing the PAPR and we call it PCT(PAPR Control Tone) method. This proposed PCT method is to assign control tones for PAPR reduction at the predefined sub-carriers. After IFFT(Inverse Fast Fourier Transform) and PAPR calculation, the OFDM data signal of the lowest PAPR is selected to transmit. Unlike the conventional method, it can cut down the computational complexity because it does not require the transmission and demodulation process of side information about the phase rotation. Furthermore, if this method is made up in parallel configuration, it can solve the time delay problem so that it can be processed in real time processing. This proposed method is compared with the conventional selected mapping(SLM) technique. We find out the PAPR reduction performance and BER when the number of control tone is 6 and nonlinear HPA is considered.

A Study on the Development of Polycube Teaching-Learning Materials for Mathematically Gifted Elementary School Students (초등 수학 영재를 위한 폴리큐브 교수.학습 자료 개발 연구)

  • Park, Ji-Young;Song, Sang-Hun
    • School Mathematics
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    • v.12 no.3
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    • pp.353-370
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    • 2010
  • The purpose of this study was threefold: (1) to select the components of spatial ability that could be associated with the implementation of a polycube task, embody the selected components of spatial ability as learning elements and develop the prototype of polycube teaching-learning materials applicable to gifted education, (2) to make a close analysis of the development process of the teaching-learning materials to ensure the applicability of the prototype, (3) to give some suggestions on the development of teaching-learning materials geared toward mathematically gifted classes. The findings of the study were as follows: As for the first purpose of the study, relevant literature was reviewed to make an accurate definition of spatial ability, on which there wasn't yet any clear-cut explanation, and to find out what made up spatial ability. After 13 components of spatial ability that were linked to a polycube task were selected, the prototype of teaching-learning materials for gifted education in mathematics was developed by including nine components in consideration of children's grade and level. Concerning the second purpose of the study, materials for teachers and students were separately developed based on the prototype, and the materials were modified and finalized in light of when selected students exerted their spatial ability well or didn't in case of utilizing the developed materials in class. And then the materials were finalized after being finetuned two times by regulating the learning type, sequence and degree of learning difficulty. Regarding the third purpose of the study, the polycube task performed in this study might not be generalizable, but there are seven suggestions on the development process of teaching-learning materials.

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Purification and Characterization of Antioxidative Peptides from Enzymatic Hydrolysate of Cod Teiset Protein (대구고니 단백질의 효소적 가수분해물로부터 항산화성 펩타이드의 분리${\cdot}$정제 및 특성)

  • KIM Se-Kwon;CHOI Yong-Ri;PARK Pyo-Jam;CHOI Jeoung-Ho;MOON Sung-Hoon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.3
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    • pp.198-204
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    • 2000
  • In order to utilize by-products which would normally be discarded in marine processing plants, cod teiset protein was hydrolyzed and antioxidative actiTity of the hydrolysate was investigated. AntioxidatiTe peptide was isolated using ultrafiltration membrane, ion-exchange chromatography on a SP-Sephadex C-25 column, gel filtration on a Sephadex G-15 column, high performance liquid chromatography on an ODS column, and capillary electrophoresis chromatography. Antioxidative activities of the cod teiset hydrolysate were compared with ${\alpha}-tocopherol$, one of the commercial antioxidant. The hydrolysate passed through a membrane with molecular weight cut-off (MWCO) 1 kDa was shown the strongest antioxidative activity, and the activity was higher $10{\%}$ as compared with ${\alpha}-tocopherol$. In addition, the peptide isolated by ion-exchange chromatography, gel filtration, and HPLC, respectively, was higher $53{\%}$ as compared with ${\alpha}-tocopherol$, and the amino acid sequence was Ser-Asn-Pro-Glu-Trp-Ser-Trp-Asn.

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Structural Analysis of the Danyang Area, Danyang Coalfield, Korea (단양지역의 지질구조)

  • Kim, Jeong Hwan;Koh, Hee Jae
    • Economic and Environmental Geology
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    • v.25 no.1
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    • pp.61-72
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    • 1992
  • The Danyang area consists of the thrust and folded sedimentary rocks of Paleozoic and Mesozoic Era. The area is bounded by major tectonic units which are the Gagdong Thrust to the west and the Okdong Fault to the east. According to the structural analyses, the area is affected by polyphase deformation. This study establishes deformational sequence in the area. Mylonite zone along the Okdong Fault corresponds to the first generation of structures ($D_1$). $D_1$-structures are discrete shear zone in the Jangsan Formation and bedding parallel extensional deformation in the Cambro-Ordovician sequences. $D_2$-structures were formed prior to the sedimentation of the Jurassic Bansong Group, which are the NW-trending fold and linear structures. After sedimentation of the Bansong Group, the area is strongly affected by the Daebo Orogeny which produces NE-trending thrusts, folds and linear structures. Earlier structures were tightened and rotated toward NE. Some thrust faults did not propagate into the Bansong Group. It is suggested either the Bansong Group acted as a decoupling horizon or rest on unconformably on the thrust faults. The area is weakly affected by $D_4$-event of which structures are E-W trending folds and faults. The Jugryeong Fault clearly cut the earlier folds and thrust faults. The rocks within the fault zone were sliced and rotated during the strike-slip movements. Block rotation and transpressional features can be commonly observed.

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A Study on the hair fashion feeling - Objecting to capital area university women students - (헤어 패션 감각(感覺)에 관(關)한 연구(硏究) - 수도권(首都圈) 대학(大學) 여학생(女學生)을 대상(對象)으로 -)

  • An, Hyeon-Kyeong;Cho, Kyu-Hwa
    • Journal of Fashion Business
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    • v.9 no.4
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    • pp.59-78
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    • 2005
  • This study aims to know the deferences of hair fashion feeling group in accordance with hair styling activities, general characteristics, life styles objecting to capital area university women students and aid to hair fashion design. So the results are as belows. 1. Frequency Analysis of Categories A. Hair fashion feeling - Natural, sexy, romantic pretty, sophisticate, ethnic are 90% in total hair fashion feeling variables in sequence of frequency, so it can be said these are in vogue. B. Hair styling activities - The objections visit the hair salon once 1-2months, spend about 42,000 won a month, perform cut & wave perm to sentimental reasens & hair style changes, determine the hair style well coordinated in her image and managed easily. In her home, they manage her hair style 12 minutes a day, spend 17,000 won to buy hair aids, do hair blow dry or pin or pony tail mainly in the morning, scarcely use the hair styling aids but if use, essence or wax mainly. And the degree of interest to hair style is high. C. General Characteristics - The objections's average age is 21.1, residence is seoul kangnam 23.3%, seoul kangbook 18.4%, other capital areas 58.4%, the degree of education is university students 94.9%, graduated student 5.1%, marriage is married 96%, unmarried 2.8%, family who live with is married are mainly man & woman and living with father & mother in low in man's, unmarried are mainly live alone & nuclear family, personal expenses a month is 300,000 won in average, income of home is 4,000,000 won a month. D. Life style - The objections are not in interest of physical exercises but if are, do yoga & health, like drama & comedies program, watch TV or meet friend in leisure time, like balad & dance music, fashion magazine, meet friend in cafe or college. 2. Relationship of hair fashion feeling & other variables Using the $x^2$-test, level p<0.05, Hair styling activities(frequency of hair salon coming in and out, ordinary time representing hair style, preferred hair styling aids, the amount of hair style interest), General Characteristics(age), Life style(leisure time) variables are meaningful.

Microarray analysis of gene expression in raw cells treated with scolopendrae corpus herbal-acupuncture solution (蜈蚣(오공) 약침액(藥鍼液)이 LPS로 처리된 RAW 세포주(細胞柱)의 유전자(遺傳子) 발현(發顯)에 미치는 영향(影響))

  • Bae, Eun-Hee;Lee, Kyung-Min;Lee, Bong-Hyo;Lim, Seong-Chul;Jung, Tae-Young;Seo, Jung-Chul
    • Korean Journal of Acupuncture
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    • v.23 no.3
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    • pp.133-160
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    • 2006
  • Objectives : Scolopendrae Corpus has a broad array of clinical applications in Korean medicine, including treatment of inflammatory conditions such as arthritis. To explore the global gene expression profiles in human Raw cell lines treated with Scolopendrae Corpus herbal-acupuncture solution (SCHAS), cDNA microarray analysis was performed. Methods : The Raw 264.7 cells were treated with lipopolysaccharide (LPS), SCHAS, or both. The primary data was normalized by the total spots of intensity between two groups, and then normalized by the intensity ratio of reference genes such as housekeeping genes in both groups. The expression ratio was converted to log2 ratio. Normalized spot intensities were calculated into gene expression ratios between the control and treatment groups. Greater than 2 fold changes between two groups were considered to be of significance. Results : Of the 8 K genes profiled in this study, with a cut-off level of two-fold change in the expression, 20 genes (BCL2-related protein A1, MARCKS-like 1, etc.) were upregulated and 5 genes (activated RNA polymerase II transcription cofactor 4, calcium binding atopy-related autoantigen 1, etc.) downregulated following LPS treatment. 139 genes (kell blood group precursor (McLeod phenotype), ribosomal protein S7, etc.) were upregulated and 42 genes (anterior gradient 2 homolog (xenopus laevis), phosphodiesterase 8B, etc.) were downregulated following SCHAS treatment. And 10 genes (yeast saccharomyces cerevisiae intergeneic sequence 4-1, mitogen-activated protein kinase 1, etc.) were upregulated and 8 genes (spermatid perinuclear RNA binding protein, nuclear receptor binding protein 2, etc.) were downregulated following co-stimulation of SCHAS and LPS. Discussions : It is thought that microarrays will play an ever-growing role in the advance of our understanding of the pharmacological actions of SCHAS in the treatment of arthritis. But further studies are required to concretely prove the effectiveness of SCHAS.

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Spatio-Temporal Variation of Polymetallic Mineralization in the Wooseok Deposit (우석광상 다금속 광화작용의 시공간적 특성변화)

  • Im, Heonkyung;Shin, Dongbok;Jeong, Junyeong;Lee, Moontaek
    • Economic and Environmental Geology
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    • v.51 no.6
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    • pp.493-507
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    • 2018
  • The Wooseok deposit in Jecheon belongs to the Hwanggangri Mineralized Distict of the northeastern Ogcheon Metamorphic Belt. Its geology consists mostly of limestone of the Choseon Supergroup and the Cretaceous Muamsa granite intruded at the eastern area of the deposit. The deposit shows vertical occurrence of skarn and hydrothermal vein ores with W-Mo-Fe and Cu-Pb-Zn mineralization and skarn is developed only at lower levels of the deposit. Skarn minerals are replaced or cut by ore minerals in paragenetic sequence of magnetite-hematite, molybdenite-scheelite-wollframite, and higher abundances of pyrrhotite-chalcopyrite-pyrite-sphalerite-galena. Garnet has chemical compositions of $Ad_{65.9-97.8}Gr_{0.3-32.0}Pyr_{0.9-3.0}$, corresponding to andradite series, and pyroxene compositions are $Hd_{4.5-49.7}Di_{42.3-93.9}Jo_{0.5-7.9}$, prevailing in diopside compositions, both of which suggest oxidized conditions of skarnization. On the FeS-MnS-CdS ternary diagram, FeS contents of sphalerite in vein ores decrease with increasing MnS contents from bottom to top levels, possibly relating to W mineralization in deep and Pb-Zn mineralization in shallow level. Sulfur isotope values of sulfide minerals range from 5.1 to 6.8‰, reflecting magmatic sulfur affected by host rocks. W-Mo skarn and Pb-Zn vein mineralization in the Wooseok deposit were established by spatio-temporal variation of decreasing temperature and oxygen fugacity with increasing sulfur fugacity from bottom to top levels.

Etiological Properties and Coat Protein Gen Analysis of Potato Virus Y Occuring in Potatoes of Korea (우리나라 감자에 발생하는 PVY의 병원학적 특성 및 외피단백질 유전자 분석)

  • ;Richard M. Bostock
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 1995.06b
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    • pp.77-96
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    • 1995
  • To obtain basic informations for the improvement of seed potato production in Korea, some etiological properties of potato virus Y(PVY) distributed in the major seed potato production area(Daekwanryeong) were characterized, and the nucleotide and amino acid sequences of the coat protein gene of the PVY strains isolated were analyzed. PVY strains in Daekwonryeong, an alpine area, were identified to be two strains, PVYo and PVYN by symptoms of indicator plants, and their distribution in potato fields was similar. Major symptom on potato varieties by PVY was grouped as either mosaic alone or mosaic accompanied with veinal necrosis in the lower leaves. The symptom occurrence of the two symptoms was similar with Irish Cobbler, but Superior showed a higher rate of mosaic symptom than the other. The PVY strain which was isolated from potato cv. Superior showing typical mosaic symptoms produced symptoms of PVY-O on the indicator plants of Chenopodium amaranticolor, Nicotiana tabacum cv. Xanthi nc and Physalis floridana, but no symptom o Capsicum annum cv. Ace. Moreover, results from the enzyme-linked immunosorbent assay with monoclonal and polyclonal antibodies showed that the isolated PVY reacts strongly with PYV-O antibodies but does not react specifically with PVY-T antibodies. The purified virus particles were flexious with a size of 730$\times$11nm. On the basis of the above characteristics, the strain was identified to be a PVY-O and named as of PVY-K strain. The flight of vector aphids was observed in late May, however, the first occurrence of infected plants was in mid June with the bait plants surrounded with PVY-infected potato plants and early July with the bait plants surrounded with PVY-free potato plants. PVY infection rates by counting symptoms on bait plants (White Burley) were 1.1% with the field surrounded with PVY-free potato plants and 13.7% the fields surrounded with PVY-infected potato plants, showing the effect of infection pressure. The propagated PVY-K strain on tobacco(N. sylvestris) was purified, and the RNA of the virus was extracted by the method of phenol extraction. The size of PVY-K RNA was measured to be 9, 500 nucleotides on agarose gel electrophoresis. The double-stranded cDNAs of PVY-K coat protein(CP) gene derived by the method of polymerase chain reaction were transformed into the competent cells of E. coli JM 109, and 2 clones(pYK6 and pYK17) among 11 clones were confirmed to contain the full-length cDNA. Purified plasmids from pYK17 were cut with Sph I and Xba I were deleted with exonuclease III and were used for sequencing analysis. The PVY-K CP gene was comprised of 801 nucleotides when counted from the clevage site of CAG(Gln)-GCA(Ala) to the stop codon of TGA and encoded 267 amino acids. The molecular weight of the encoded polypeptides was calculated to be 34, 630 daltons. The base composition of the CP gene was 33.3% of adenine, 25.2% of guanine, 20.1% of cytosine and 21.4% of uracil. The polypeptide encoded by PVY-K CP gene was comprised of 22 alanines, 20 threonines, 19 glutamic acids and 18 glycines in order. The homology of nucleotide sequence of PVY-K CP gene with those of PVY-O(Japan), PVY-T(Japan), PVY-TH(Japan), PVYN(the Netherlands), and PVYN(France) was represented as 97.3%, 88.9%, 89.3%, 89.6% and 98.5%, respectively. The amino acid sequence homology of the polypeptide encoded by PVY-K CP gene with those encoded by viruses was represented as 97.4%, 92.5%, 92.9%, 92.9%, and 98.5%, respectively.

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