• 한국축산식품학회지
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• 제38권4호
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• pp.829-834
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• 2018

The objective of this study was to determine the minimum enrichment time for different types of food matrix (pork, beef, and fresh-cut lettuce) in an effort to improve Escherichia coli detection efficiency. Fresh pork (20 g), beef (20 g), and fresh-cut lettuce (20 g) were inoculated at 1, 2, and 3 Log CFU/g of Escherichia coli. Samples were enriched in filter bags for 3 or 5 h at $44.5^{\circ}C$, depending on sample type. E. coli cell counts in the samples were enriched in E. coli (EC) broth at 3 or 5 h. One milliliter of the enriched culture medium was used for DNA extraction, and PCR assays were performed using primers specific for uidA gene. To detect E. coli (uidA) in the samples, a 3-4 Log CFU/mL cell concentration was required. However, E. coli was detected at 1 Log CFU/g in fresh pork, beef, and fresh-cut lettuce after 5, 5, and 3-h enrichment, respectively. In conclusion, 5-h enrichment for fresh meats and 3-h enrichment for fresh-cut lettuce in EC broth at $44.5^{\circ}C$, and PCR analysis using uidA gene-specific primers were appropriate to detect E. coli rapidly in food samples.

• Asian Pacific Journal of Cancer Prevention
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• 제15권1호
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• pp.363-368
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• 2014

Determination of the cause of malignant pleural effusions is important for treatment and management, especially in cases of unknown primaries. There are limited biomarkers available for prediction of the cause of malignant pleural effusion in clinical practice. Hence, we evaluated pleural levels of five tumor biomarkers (CEA, AFP, CA125, CA153 and CA199) in predicting the cause of malignant pleural effusion in a retrospective study. Kruskal-Wallis or Mann-Whitney U tests were carried out to compare levels of tumor markers in pleural effusion among different forms of neoplasia - lung squamous cell carcinoma, adenocarcinoma, or small cell carcinoma, mesothelioma, breast cancer, lymphoma/leukemia and miscellaneous. Receiver operator characteristic analysis was performed to evaluate sensitivity and specificity of biomarkers. The Kruskal-Wallis test showed significant differences in levels of pleural effusion CEA (P<0.01), AFP (P<0.01), CA153 (P<0.01) and CA199 (P<0.01), but not CA125 (P>0.05), among the seven groups. Receiver operator characteristic analysis showed that, compared with other four tumor markers, CA153 was the best biomarker in diagnosing malignant pleural effusions of lung adenocarcinoma (area under curve (AUC): 0.838 (95%confidence interval: 0.787, 0.888); cut-off value: 10.2U/ml; sensitivity: 73.2% (64.4-80.8)%, specificity: 85.2% (77.8-90.8)%), lung squamous cell carcinoma (AUC: 0.716 (0.652, 0.780); cut-off value: 14.2U/ml; sensitivity: 57.6% (50.7-64.3)%, specificity: 91.2% (76.3-98.0)%), and small-cell lung cancer (AUC: 0.812 (0.740, 0.884); cut-off value: 9.7U/ml; sensitivity: 61.5% (55.0-67.8)%, specificity: 94.1% (71.2-99.0)%); CEA was the best biomarker in diagnosing MPEs of mesothelioma (AUC: 0.726 (0.593, 0.858); cut-off value: 1.43ng/ml; sensitivity: 83.7% (78.3-88.2)%, specificity: 61.1% (35.8-82.6)%) and lymphoma/leukemia (AUC: 0.923 (0.872, 0.974); cut-off value: 1.71ng/ml; sensitivity: 82.8% (77.4-87.3)%, specificity: 92.3% (63.9-98.7)%). Thus CA153 and CEA appear to be good biomarkers in diagnosing different causes of malignant pleural effusion. Our findings implied that the two tumor markers may improve the diagnosis and treatment for effusions of unknown primaries.

• 한국콘텐츠학회논문지
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• 제11권4호
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• pp.414-427
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• 2011

본 연구는 컷오프 수준에 따라 활성화 셀(cell) 수와 래더(ladder) 수 등의 통계량의 변화를 살펴보고, 컷오프 수준의 변화에 따른 가치 맵이 어떻게 변화하는지를 제시하며, 주어진 조건(100명의 응답자에게 최대 5개의 중요한 속성을 선택하도록 하여 가치사슬을 만든 후 이를 이용하여 가치 맵을 그렸을 경우)하에서 정보손실을 최소화하면서 설명력이 있는 최적의 컷오프 수준 결정과 이에 따른 가치 맵을 표현하고자 한다.

• 한국해양바이오학회지
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• 제7권2호
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• pp.52-57
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• 2015

The purpose of this study was to investigate a relationship between ultraviolet radiation and initial cell density (ICD) of microalgae using a floating marine photobioreactor (PBR). To examine the effect of ultraviolet (UV) radiation in sunlight on biomass productivity as a function of ICD, 0.5-L floating PBRs covered with or without UV cut-off film were placed in an outdoor rectangular tank containing 200 L of water. At the lower ICDs, 0.01 and 0.05 g/L, biomass productivities in the PBRs without UV cut-off film decreased by $278{\pm}21%$ and $222{\pm}3%$ compared with those with the film, respectively. In contrast, the presence of UV cut-off film did not have a significant effect on biomass productivities at the higher ICDs, 0.25 and 1.25 g/L. When the differences in biomass productivity made by the UV cut-off film were plotted against the sum of cell projection area per light receiving area of the PBR, the results revealed that the inhibitory effect of UV on biomass productivity can be negligible when the sum of cell projection area is equal to the light receiving area of the PBR. These results show that photoinhibition caused by UV radiation could be eliminated via operating the PBR with a proper ICD.

• 유체기계공업학회:학술대회논문집
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• 유체기계공업학회 2001년도 유체기계 연구개발 발표회 논문집
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• pp.419-424
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• 2001

TCV(Temperature control valve by pressure compensation) controls temperature constantly, when it is sending steam or high temperature water to heating device of heat exchanger. For designing TCV, the ratio of piston and hole diameters is one of the important design parameters. Numerical analysis is carried out to elucidate the flow characteristics in the TCV with different port areas of cold and hot waters, using the k-$\epsilon$ turbulence model and Cartesian cut-cell method. Numerical results show that the exit flow rate is mainly affected by pressure distribution in the piston.

• 한국CDE학회논문집
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• 제13권6호
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• pp.450-458
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• 2008

Recently, Cartesian grid approach has been popular to generate grid meshes for complex geometries in CFD (Computational Fluid Dynamics) because it is based on the non-body-fitted technique. This paper presents a method of an octree generation and boundary cell clipping using section curves for fast octree generation and elimination of redundant intersections between boundary cells and triangles from 3D triangular mesh. The proposed octree generation method uses 2D Scan-Converting line algorithm, and the clipping is done by parameterization of vertices from section curves. Experimental results provide octree generation time as well as Cut-cell clipping time of several models. The result shows that the proposed octree generation is fast and has linear relationship between grid generation time and the number of cut-cells.

• 대한토목학회논문집
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• 제29권2B호
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• pp.121-129
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• 2009

본 연구는 효율적이며 정확한 격자생성기법인 분할격자기법을 이용하여 댐붕괴 흐름을 수치모의한다. 분할격자기법은 부분적으로 비구조격자를 사용하지만, 대부분의 흐름영역을 균일한 크기의 Cartesian 격자로 이산화한다. HLLC Riemann 근사해법과 TVD-WAF기법의 유한체적기법을 적용하여 흐름률을 계산하고 분할격자의 영역을 위한 수치모형을 구성한다. 수치모형을 검증하기 위하여 이상적인 하도에서의 정상류, 불균일하도에 의해 형성되는 정상류 및 사각형수조의 자유진동흐름을 모의하여 해석해와 비교하였다. 마지막으로, 실험수로에서 발생한 댐붕괴파의 흐름을 모의하여 관측값과 비교하여 정확하고 안정된 결과를 확인하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권3호
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• pp.1803-1806
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• 2013

Purpose: Carbohydrate antigen (CA) 242 is inversely related to prognosis in many cancers. However, few data regarding CA 242 in esophageal cancer (EC) are available. The aim of this study was to determine the prognostic value of CA 242 and propose an optimum cut-off point in predicting survival difference in patients with esophageal squamous cell carcinoma (ESCC). Methods: A retrospective analysis was conducted of 192 cases. A receiver operating characteristic (ROC) curve for survival prediction was plotted to verify the optimum cuf-off point. Univariate and multivariate analyses were performed to evaluate prognostic parameters for survival. Results: The positive rate for CA 242 was 7.3% (14/192). The ROC curve for survival prediction gave an optimum cut-off of 2.15 (U/ml). Patients with CA 242 ${\leq}$ 2.15 U/ml had significantly better 5-year survival than patients with CA 242 >2.15 U/ml (45.4% versus 22.6%; P=0.003). Multivariate analysis showed that differentiation (P=0.033), CA 242 (P=0.017), T grade (P=0.004) and N staging (P<0.001) were independent prognostic factors. Conclusions: Preoperative CA 242 is a predictive factor for long-term survival in ESCC, especially in nodal-negative patients. We conclude that 2.15 U/ml may be the optimum cuf-off point for CA 242 in predicting survival in ESCC.

• Nuclear Engineering and Technology
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• 제53권4호
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• pp.1157-1166
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• 2021

In this study, a numerical sensitivity analysis was performed to determine the fire suppression time for a large number of water mist nozzles in a large fire compartment. Fire simulations were performed using FDS (Fire dynamics simulator) 6.5.2 under the same condition as the test scenario 5 of the International Maritime Organization (IMO) 1165 test protocol. The sensitivities of input parameters including cell size, extinguishing coefficient (EC), droplets per second (DPS), and peak heat release rate (HRR) of fuel were investigated in terms of the normalized HRR and temperature distribution in the compartment. A new method of determining the fire suppression time using FDS simulation was developed, based on the concept of the cut-off time by cut-off value (COV) of the heat release rate per unit volume (HRRPUV) and the cooling time by the HRR cooling time criteria value (CTCV). In addition, a method was developed to determine the average EC value for the simulation input, using the cooling time and cut-off time.

• 한국수자원학회논문집
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• 제44권7호
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• pp.511-522
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• 2011

적응적 메쉬세분화기법과 분할격자기법을 적용한 2차원 천수방정식모형을 활용하여 구조물을 고려한 극한 홍수 실험을 모의하였다. 본 연구에 사용된모형은 두 격자생성 기법을함께 사용함으로서 복잡한 경계를보다 적은 격자로 효율적으로 표현하는 것이 가능하며, 동적 적응 메쉬세분화기법을 사용하여 흐름이 빠르게 변하는 영역에서 정확도를 유지하면서도 효율적으로 계산하는 것이 가능하다. HLLC 리만근사해법과 MUSCL 기법을 적용하여 시공간상에서 2차정도를 유지하며, 댐붕괴파와 같은 불연속적인 흐름을 정확하게 모의할 수 있다. 모형의 검증을 위해 IMPACT 프로젝트에서 수행한 도시지역 극한홍수실험을 모의하였다. 실험결과와 모의결과가 양호하게 일치하는 것을 확인하였으며, 천이류 현상과 함께 구조물에 의한 홍수파 전달 양상이 의미있는 수준으로 모의된 것을 확인하였다. 또한 분할격자기법의 사용으로 모델의 격자 민감도가 향상되었다. 본 모델은 댐붕괴와 같이 내수침수현상이 지배적이지 않은 도시범람을 모의하는데 활용할 수 있을 것으로 기대된다.