• Journal of Microbiology and Biotechnology
• /
• v.9 no.6
• /
• pp.764-772
• /
• 1999

To develop an enzyme-linked immunosorbent assay (ELISA) for detecting mold, we produced anti-mold polyclonal antibodies by immunizing extracellular polysaccharide (EPS) of Aspergillus flavus or Penicillium citrinum in rabbits subcutaneously. Using the purified antibody (Ab) and Ab-HRP conjugate, a sandwich ELISA for EPS was established. The standard curve of the ELISA showed the detection limit for P citrinum EPS to be $0.003{\;}\mu\textrm{g}/ml$. The cross-reactivities of the anti-P citrinum EPS Ab toward components of P citrinum such as EPS, liquid, and solid culture mycelium were 100, 10.5, and 0.58%, respectively, and those toward components of A. flavus such as EPS, liquid and solid culture mycelium, and spore were 300, 0.67, 0.29, and 0%, respectively. When the reactivities toward culture broths of 59 mold strains were tested by the sandwich ELISA, most of the Aspergillus (16 of 18) and Penicillium (14 of 16) strains along with one of the two Cladosporium strains gave positive signals in the culture broths even when diluted 1,000 fold, while the rest of species such as Fusarium, Absidia, Alternaria, and Candida gave negative signals. When the water extracts of 30 corn samples were analyzed by the sandwich ELISA, the EPS in the com could be detected in the concentration range of $0.1-1.6{\;}\mu\textrm{g}/g$.

• Journal of KIISE:Computer Systems and Theory
• /
• v.32 no.11_12
• /
• pp.705-716
• /
• 2005

There exist many feature point detection algorithms that developed in pattern recognition research . However, interactive applications for the pen-input displays such as Tablet PCs and LCD tablets have set different goals; reliable segmentation for different drawing styles and real-time on-the-fly fieature point defection. This paper presents a curvature estimation method crucial for segmenting freeHand pen input. It considers only local shape descriptors, thus, peforming a novel curvature estimation on-the-fly while drawing on a pen-input display This has been used for pen marking recognition to build a 3D sketch-based modeling application.

• Microbiology and Biotechnology Letters
• /
• v.22 no.4
• /
• pp.430-435
• /
• 1994

In order to develop an enzyme-linked immunosorbent assay (ELISA) for zearalenone (ZEA) in corn, we produced antisera by immunizing rabbits with ZEA-6'-carboxymethyloxime-BSA, purified polyclonal anti ZEA antibodies, and subsequently established a competitive indirect ELISA. The antibodies showed low cross-reactivity of 9.6~1.4% against ZEA analogues such as $\alpha$-zearalenol, $\beta$-zearalenol, $\alpha$-zearalanol, and $\beta$-zearalanol. From the standard curve of the ELISA for ZEA in corn, the detection range was found to be 0.3~1, 000 ng/ml. When artificially contaminated corns were assayed by the ELISA, the average recovery of ZEA spiked to 30~1, 000 ng/g was 109% (96~123%), although that of ZEA spiked to 10 ng/g was somewhat high (258%). The average coefficient of variation (CV) of the recovery was 18.0% (0.9~28.3%). When 9 corn samples naturally contaminated were assayed 3 times, the average CV of the determinitions was 27.7% (9.3~52.4%). Therefore, the ELISA was elucidated to be a practical tool for the detection of ZEA of 30 ng/g and more from corn.

• The Journal of Korean Medicine
• /
• v.18 no.1
• /
• pp.143-156
• /
• 1997

This report was conducted to quantify the pulse/respiration ratio and set up the normal range of wan-maeck(緩脈). In order to objectify the pulse diagnosis and use as basic clinical index of Cold-Hot diagnosis, we developed the hardware and software for detection and interpretation of pulse/respiration ratio, pulse/expiration ratio, pulse/respiration ratio, inspiration time, expiration time, respiration frequency, respiration time, duration of one pulse and pulse and pulse rate per minute, The results were as follows; pulse/respiration ratio is $4.30{\pm}1.03$ times, pulse/respiration ratio is $1.60{\pm}0.32$ times, pulse/respiration ratio is $2.37{\pm}0.75$ times, inspiration time is $1.35{\pm}0.20$ sec, expiration time is $1.89{\pm}0.39$ sec, respiration frequency is $17.16{\pm}3.49$ times/min, total respiration time is $3.63{\pm}0.71$ sec, duration of a pulse is $0.86{\pm}0.15$ sec, pulse rate is $71.51{\pm}12.30$ times/min.

• Journal of the Korean Chemical Society
• /
• v.43 no.4
• /
• pp.430-437
• /
• 1999

The determination of total chlorine residuals in drinking water by flow injection analysis(FIA) with iodometric UV detection was investigated. The pH of the acid stream, the concentration of the iodide ion,the length of the mixing and reaction coils, the injection sample size, and flowrate were optimized as parameters for determining total chlorine residuals by FIA method. lodide was selectively oxidized to iodine by hypochlorite at pH 8.3 Ethylenediamine as masking agent for masking interference ions from the sample was given the best efficency. Calibration curve presented linear range of 0.03-3 mg/L for hypochlorite ion with a correlation coefficient of 0.999 or better. The detection limit was found to be 0.007 mg/L for hypochlorite ion. Under these analytical conditions, total chlorine residuals in several tap water sampled in the city of Jeonju were analyzed.

• Bulletin of the Korean Chemical Society
• /
• v.24 no.1
• /
• pp.70-74
• /
• 2003

The heterogeneous bioluminescence immunoassay for digoxin was developed using photoprotein, native aequorin as a label and the site-directed immobilization technique based on avidin/biotin interaction. Aequorin is a bioluminescence protein, originally isolated from the jellyfish Aequoria Victoria and an attractive label in analytical applications because of sensitive detection due to virtually no background bioluminescent signal. Digoxin is a cardioactive drug, and its therapeutic level in serum is at low concentration with very narrow therapeutic index. The aequorin-digoxigenin conjugates were synthesized by the N-hydroxysuccinimide ester method and characterized in terms of bioluminescent residual activity. The resulting dose-response curve shows that the detection limit is $1.0\;{\times}\;10^{-10}\;M$ and a dynamic range is three orders of magnitude, which was obtained by $1.0\;{times}\;10^{-10}\;M$ conjugate and 0.9 μg/mL anti-digoxin antibody. Three structurally similar molecules to digoxin were examined for their cross-reactivity. None of these three compounds showed any crossreactivity with digoxin antibody employed in this study. Standard amounts of digoxin corresponding to the therapeutic range were spiked into the each serum solution. Study of the serum matrix effect indicated that correlation coefficient shows good agreement between luminescence light intensity between in buffer and in serum.

• Transactions of the Korean Society for Noise and Vibration Engineering
• /
• v.26 no.6_spc
• /
• pp.651-659
• /
• 2016

Condition monitoring (CM) encounters a large data problem due to sensors that measure vibration data with a continuous, and sometimes, high sampling rate. In this study, compressive sensing approaches for condition monitoring are proposed to demonstrate the efficiency in handling a large amount of data and to improve the damage detection capability of the current condition monitoring process. Compressive sensing is a novel sensing/sampling paradigm that takes much fewer samples compared to traditional sampling methods. For the experiments a built-in rotating system was used and all data were compressively sampled to obtain compressed data. Optimal signal features were then selected without the reconstruction process and were used to detect and classify damage. The experimental results show that the proposed method could improve the data processing speed and the accuracy of condition monitoring of rotating systems.

• Journal of the Korean Society of Hazard Mitigation
• /
• v.9 no.5
• /
• pp.9-13
• /
• 2009

Modal parameters including resonant frequencies and mode shapes are heavily utililized in most damage identification throries for structural health monitoring. However, extracting modal parameters from dynamic responses needs postprocessing which inevitably involves errors in curve-fitting resonants as well as transforming the domain of responses. In this paper, the applicability of a damage identification method based on free vibration responses to the in-sevice responses is experimentally verified. The experiment is performed via applying periodic and nonperiodic moving loads to a simply supported beam and displacement responses are measured. The moving load is simulated using steel balls and a downhill device. The damage identification results show that the in-service response may be applicable to identifying damage in the beam.

• The Transactions of The Korean Institute of Electrical Engineers
• /
• v.68 no.1
• /
• pp.102-107
• /
• 2019

This paper proposes a method to improve the mass spectral detection performance of the residual gas analyzer. By improving the mode estimation method for setting the threshold value and improving the additive noise elimination method, it is possible to detect mass spectrums having low peak values of the threshold level difficult to distinguish from noise. Ion signal blocks for each mass index with noise removed by the improved method are effective for eliminating invalid ion signals based on the linear and quadratic fittings. The mass spectrum can be obtained from the quadratic fitted curves for the reconstructed ion signal block using only the valid ion signals. In addition, the resolution of the mass spectrum can be improved by correcting the error caused by the shift of the spectral peak position. To verify the performance of the proposed method, computer simulations were performed using real ion signals obtained from the residual gas analysis system under development. The simulation results show that the proposed method is valid.

• Journal of Astronomy and Space Sciences
• /
• v.36 no.2
• /
• pp.97-103
• /
• 2019

An automated signal-acquisition method for the NASA's space geodesy satellite laser ranging (SGSLR) system is described as a selection of two system parameters with specified probabilities. These parameters are the correlation parameter: the minimum received pulse number for a signal-acquisition and the frame time: the minimum time for the correlation parameter. The probabilities specified are the signal-detection and false-acquisition probabilities to distinguish signals from background noise. The steps of parameter selection are finding the minimum set of values by fitting a curve and performing a graph-domain approximation. However, this selection method is inefficient, not only because of repetition of the entire process if any performance values change, such as the signal and noise count rate, but also because this method is dependent upon system specifications and environmental conditions. Moreover, computation is complicated and graph-domain approximation can introduce inaccuracy. In this study, a new method is proposed to select the parameters via a conditional equation derived from characteristics of the signal-detection and false-acquisition probabilities. The results show that this method yields better efficiency and robustness against changing performance values with simplicity and accuracy and can be easily applied to other satellite laser ranging (SLR) systems.