Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enzyme-Linked Immunosorbent Assay for Zearalenone in Corn

옥수수 중의 Zearalenone 분석을 위한 효소번역측정법

  • 손동화 (한국식품개발연구원 이화학연구부) ;
  • 한성민 (농업생물신소재연구센터, 서울대학교 농업생명과학대학) ;
  • 임선희 (농업생물신소재연구센터, 서울대학교 농업생명과학대학) ;
  • 이인원 (농업생물신소재연구센터, 서울대학교 농업생명과학대학) ;
  • 강신영 (충북대학교 수의학과)
  • Published : 1994.08.01
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Abstract

In order to develop an enzyme-linked immunosorbent assay (ELISA) for zearalenone (ZEA) in corn, we produced antisera by immunizing rabbits with ZEA-6'-carboxymethyloxime-BSA, purified polyclonal anti ZEA antibodies, and subsequently established a competitive indirect ELISA. The antibodies showed low cross-reactivity of 9.6~1.4% against ZEA analogues such as $\alpha$-zearalenol, $\beta$-zearalenol, $\alpha$-zearalanol, and $\beta$-zearalanol. From the standard curve of the ELISA for ZEA in corn, the detection range was found to be 0.3~1, 000 ng/ml. When artificially contaminated corns were assayed by the ELISA, the average recovery of ZEA spiked to 30~1, 000 ng/g was 109% (96~123%), although that of ZEA spiked to 10 ng/g was somewhat high (258%). The average coefficient of variation (CV) of the recovery was 18.0% (0.9~28.3%). When 9 corn samples naturally contaminated were assayed 3 times, the average CV of the determinitions was 27.7% (9.3~52.4%). Therefore, the ELISA was elucidated to be a practical tool for the detection of ZEA of 30 ng/g and more from corn.

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References

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