• 제목/요약/키워드: Cultures

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Hydrocarbon Uptake Modes에 따른 유류분해 미생물 혼합체의 원유분해능 (Effect of Hydrocarbon Uptake Modes on Oil Degradation Rate by Mixed Cultures of Petroleum Degraders)

  • 고성환;이홍금;김상진
    • KSBB Journal
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    • 제13권5호
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    • pp.606-614
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    • 1998
  • In this study, biodegradation rate of Arabian light crude oil by mixed cultures of selected petroleum-degraders was determined. Their modes of hydrocarbon uptake were then observed to determine whether there are differences in biodegradation rate by the mixed cultures. By the mixed cultures of petroleum-degraders having same modes of hydrocarbon uptake, such as strain US1 and K1 (using pseudo-solubilized hydrocarbons by a biosurfactants), K2-2 and P1(using hydrocarbons by direct contact), CL 180 and IC-10 (mixed type of uptake modes), the biodegradation rates of aliphatics in the crude oil were increased more than those by their pure cultures, about 40%, 25% and 20%, respectively. Biodegradation rate of strain KH3-2 (using only water- dissolved hydrocarbons) was increased by mixed cultures with strain K1, CL180 or IC-10 possessing high emulsifying activity. However, the biodegradation rate of the crude oil was decreased about 20%-40% by the mixed cultures of petroleum-degraders having different mode of hydrocarbon uptake, such as addition of strain US1 or K1 in the cultures of K2-2 or P1. Biosurfactants produced by US1 or K1 seems to enhance the emulsification of crude oil in aqueous phase but inhibit the attachment of K2-2 or P1 to crude oil. As same phenomena, the addition to Triton X-100 into the culture of strain US1, K1, CL180, IC-10 or KH3-2 increased the biodegradation rate, but the addition in the culture of strain K2-2 or P1 decreased the biodegradation rate. The mixed culture made of CL180, IC-10 and KH3-2 degraded 61.5% of aliphatics and 69% of aromatics in 3% (v/v) of Arabian light crude oil added.

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동아시아 상호 이해를 위한 문화 다양성 교육의 시론적 연구 -"인간관계와 생활습관" 문화를 중심으로- (A Study on East Asia Cultural Diversity in Geography Education: Centered on "Human Relationship and Living-Habit" Culture)

  • 김다원
    • 대한지리학회지
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    • 제46권1호
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    • pp.100-114
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    • 2011
  • 본 연구는 동아시아 지역이 유교 문화권으로 일컬어져 온 지역이지만 유교 문화권 안에서 발달했던 가족 중심의 문화, 전통 문화, 대인 관계 등의 인간관계와 생활습관 문화에서 차이가 있음을 전제하고, 앞으로 국가간 교류의 활성화와 원활한 의사소통, 인간 관계 형성을 위해서는 동아시아 지역의 문화 다양성을 인식하고 이해하는 교육으로의 전환이 필요함을 제시하였다. 그리고 지리수업에서 동아시아 문화 다양성 교육을 위한 내용을 구성하여 제시하였다. 교과와의 연계성을 높이기 위하여 2009개정 교육과정 지리 영역 단원과 단원별 성취 기준을 분석하였고 분석에 의거하여 초, 중, 고등학교 수준에 적합한 학습 내용을 구성하여 제시하였다.

발효된 물김치인 동치미에서 분리한 혼합 젖산균의 생리활성 증진에 대한 연구 (Studies on the Enhanced Physiological Activities of Mixed Lactic Acid Bacteria Isolated from Fermented Watery Kimchi, Dongchimi)

  • 최문섭;김동민;오계헌
    • KSBB Journal
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    • 제30권5호
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    • pp.245-252
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    • 2015
  • The aim of this study was to investigate the efficacy of enhanced physiological activities in cultures isolated from Korean fermented watery Kimchi, Dongchimi, of single lactic acid bacteria (LAB), and when these three are mixed LAB as probiotics. Using the BIOLOG system and 16S rRNA sequencing, the isolates were characterized, and identified and assigned to Leuconostoc mesenteroides DK-3, Leuconostoc dextranicum DK-6, and Lactobacillus curvatus DK-13, respectively. Growth rate and pH changes, production of organic acids as metabolites, and physiological activities of the single and mixed LAB cultures, were monitored and compared. In mixed LAB cultures after 72 h of incubation, the maximum concentrations of lactic acid and acetic acid were approximately 340.5 mM and 191.9 mM, respectively, and pH changed from 7.00 to 3.62. Mixed LAB cultures were able to eliminate 96.3% of nitrite. Activities of antioxidant and ${\beta}$-galactosidase were 60.3% and 16.8 units/mg, respectively. Significant antibacterial activity of the concentrated supernatants was demonstrated against several food-poisoning bacteria. Physiological activities obtained from the mixed LAB cultures have been shown to be considerably higher than those of single LAB cultures. In conclusion, these studies demonstrate that compared to the single cultures, all physiological activities in mixed LAB cultures are significantly enhanced.

1974년(年) 한국(韓國)에서 분리(分離)된 쌀모넬라 균(菌)에 관(關)한 보고(報告) (A report on the Salmonella cultures isolated in Korrea(1974))

  • 김영자;정경석;전남호;유영해
    • 대한미생물학회지
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    • 제10권1호
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    • pp.9-12
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    • 1975
  • 1974년도(年度) 1년간(年間) 각시도(各市道) 위생시험소(衛生試驗所)와 보건소(保健所) 등(等)에서 검사(檢査) 의뢰된 검체(檢體) 1504건중(件中)에서 717주(株)의 Salmonella 균속(菌屬)을 분리(分離) 동정(同定)하였다. 동정(同定)된 717주중(株中)에서 Salmonella Typhi가 698주(株)로 대부분을 차지하고 있었으며 그 외(外)의 Salmonella 균주(菌株)가 19주(株)이 었는데 이들을 seretype별(別)로 나누어 보면 다음과 같다. Salmonella Group A S. parathyphi A 7 주(株) Salmonella Group B S. paratyphi B 1 주(株) S. typhimurium 6 주(株) Salmonella Group C S. thompson 1 주(株) Salmonella Group D S. typhi 698 주(株) S. enteritidis 4 주(株) 항생물질(抗生物質)에 대(對)한 감수성시험(感受性試驗) 결과(結果) ampicillin에 대(對)한 감수성균(感愛性菌)이 99.6%이었고 chloramphenicol에 대(對)한 감수성균(感受性菌)이 93.4%이었으며 gentamycin에 대(對)하여서는 88.6%가 감수성균(感受性菌)이었고 streptomycin에 대(對)한 감수성균(感受性菌)이 49.7%이었으며 tetracycline에 대(對)하여서는 42.3%가 감수성균(感受性菌)이었다.

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LED (Light Emitting Diode)를 이용한 Nannochloropsis sp.의 성장 및 생화학적 조성 변화 (Changes in the Growth and Biochemical Composition of Nannochloropsis sp. Cultures Using Light-Emitting Diodes)

  • 박진철;권오남;홍성익;안희춘;배재현;박미선;박흠기
    • 한국수산과학회지
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    • 제46권3호
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    • pp.259-265
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    • 2013
  • The marine microalgae Nannochloropsis sp. was cultured under a metal halide lamp (MH) or light-emitting diodes (LEDs) of various wavelengths (blue, LB; red, LR; yellow, LY; green, LG; white, LW); changes in growth, total carotenoid, chlorophyll a, amino acid and fatty acid profiles were investigated. LB-exposed cultures exhibited the highest specific growth rate (SGR) (0.32), whereas LY-exposed cultures showed the lowest SGR (0.18). After cultivation for 9 days, the maximum dry cell weight (g/L) of LB-exposed cultures was significantly higher than that of those exposed to other light conditions (LB>MH>LW$${\geq_-}LG{\geq_-}$$LR>LY). The essential amino acid (EAA, %) contents of cultures exposed to LG, LB, LR and MH were higher than those exposed to LY and LW (P<0.05). Eicosapentaenoic acid and n-3 highly unsaturated fatty acid levels were significantly higher in MH-exposed cultures compared to those exposed to LY (P<0.05), with no marked difference compared to those exposed to LB, LG, LR and LW (P>0.05). The total carotenoid content was highest in LR-exposed cultures (18.0 mg/L), whereas MH showed the lowest (11.8 mg/L; P<0.05). Chlorophyll a content was highest in cultures exposed to LR compared to other light sources. These results suggest consistent differences in growth and biochemical composition after exposure to light of different wavelengths.

The Role of Enzymes Produced by White-Rot Fungus Irpex lacteus in the Decolorization of the Textile Industry Effluent

  • Shin, Kwang-Soo
    • Journal of Microbiology
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    • 제42권1호
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    • pp.37-41
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    • 2004
  • The textile industry wastewater has been decolorized efficiently by the white rot fungus, Irpex lacteus, without adding any chemicals. The degree of the decolorization of the dye effluent by shaking or stationary cultures is 59 and 93%, respectively, on the 8th day. The higher level of manganese-dependent peroxidase (MnP) and non-specific peroxidase (NsP) was detected in stationary cultures than in the cultures shaken. Laccase activities were equivalent in both cultures and its level was not affected significantly by the culture duration. Neither lignin peroxidase (LiP) nor Remazol Brilliant Blue R oxidase (RBBR ox) was detected in both cultures. The absorbance of the dye effluent was significantly decreased by the stationary culture filtrate of 7 days in the absence of Mn (II) and veratryl alcohol. In the stationary culture filtrate, three or more additional peroxidase bands were detected by the zymogram analysis.

성공적인 국제거래를 위한 인도상인과의 협상전략에 관한 연구 (Understanding negotiating dynamics across cultures by Indian businessmen)

  • 박양섭
    • 무역상무연구
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    • 제43권
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    • pp.457-480
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    • 2009
  • The objective of this study is to understand negotiating dynamics across cultures by Indian businessmen and suggest proper negotiating strategies to Korean businessmen when they make a deal with Indian firms. The study proposes, as a result, twelve countermeasures as effective negotiating strategies against negotiating cultures of Indian businessmen. Better understanding on business cultures of the country with which Korea makes international transactions leads more fruitful outcome in concluding the international agreement. So cross-cultural studies are very much required for successful negotiations. Recently many Korean firms want to try to penetrate into Indian market and do successful operation because India becomes a compulsory subject for most of Korean firms. To increase possibilities of success in India, such Korean firms may consider applying the suggested negotiating strategies to the Indian business fields.

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