• Korean Journal of Medicinal Crop Science
• /
• v.6 no.1
• /
• pp.62-69
• /
• 1998

There was no significant difference in length and width of leaf and number of leaves per plant between tissue-cultured plants and conventionally propagated ones but chlorophyll content increased in tissue-cultured ones. Percent of sprouting from planted root segments significantly increased in tissue-cultured plants, resulting in yield increase of more than 200% per 10a. Root thickness of tissue-cultured plants at the time of planting influenced percent of sprouting and yield. Plants with root diameter ranging from 3 to 6mm gave good yield. When virus infection was monitored with N. tabacum and C. amaranticolor as indicator plants, 100% infection occurred in vegetatively propagated plants and introduced plants from China. whereas plants obtained from apical meristem showed 0% and 40% to 45% infection in vitro plantlets and 1 year old plants in vivo, respectively. Tobamovirus and unidentified virus particles were detected in electron microscopy.

• Horticultural Science & Technology
• /
• v.30 no.2
• /
• pp.201-207
• /
• 2012

This study was conducted to investigate the effect of supporting material and ionic strength of the MS medium on growth of strawberry plantlets cultured in vitro for the rapid mass production. Explants of $Fragaria$ $ananassa$ 'Houkouwase' in vitro were planted in the agar or Tosilee (commercial plug medium) medium as the supporting material and supplied with 1/4 MS, 1/2 MS or basal (as the control) MS medium in an autotrophic micropropagation. Plant height and root length were significantly greater when they were cultured in 1/2 MS medium as compared to those grown in the agar medium. Also, shoot fresh and dry weights, and leaf area in the 1/2 MS medium were greater than in 1/4 MS or basal MS medium. When plantlets were cultured in Tosilee medium and fed with the basal MS medium, plant height, root length, shoot fresh and dry weights, root fresh and dry weights, and leaf area were promoted and greater than those in plantlets cultured in the agar medium.

• Journal of Plant Biotechnology
• /
• v.5 no.2
• /
• pp.115-119
• /
• 2003

To determine the appropriate concentrations of nutrients and growth regulators for shoot proliferation and root initiation, several rose hybrid tea cultivars were cultured. Cultured shoot tips and lateral buds from different cultivars proliferated multiple shoots on Murashige and Skoog (MS) medium supplemented with 0 to 4 mg/L BA and 0 to 0.05 mg/L NAA. The ability of the explants to proliferate shoots and initiate roots was affected by genotype, the nodal position of explant, the strength of MS basal medium and growth regulators used. The buds nearest the apex exhibited the slowest rate of development. Most cultivars had the highest shoot proliferation when cultured on MS medium with 2 mg/L BA and 0.01 mg/L NAA, but the degree varied by cultivars. Root development was enhanced by lowering the concentration of MS salts.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.22 no.6
• /
• pp.1482-1486
• /
• 2008

The antihypertensive effects of the Wild Mountain Ginseng Cultured Root Extract (WMGCE) were investigated in spontaneously hypertensive rats (SHR). Daily oral administration of the WMGCE (100 and 200 mg/kg) exhibited a significant decrease in blood pressure in SHR rats during for 8 weeks. The systolic blood pressure was dose- and time-dependently decreased significantly from the second weeks (p < 0.05) to the end of WMGCE treatment in SHR. The WMGCE decreased the plasma levels of sodium, potassium, chlorides, urea and osmolarity in SHR rats but no statistically significant change was observed. Furthermore, no significant changes were noted on heart weight, heart rate and diameter of aorta after WMGCE treatment in SHR. Our results suggest that daily oral administration of WMGCE at the dose of 100 and 200 mg/kg for 8 weeks exhibited antihypertensive activities.

• Korean Journal of Pharmacognosy
• /
• v.34 no.2 s.133
• /
• pp.179-184
• /
• 2003

We have investigated the effect of MeOH extract of the adventious root culture of Panax ginseng on hyperlipidemic rats induced by fat-rich diet. We also analyzed and compared the content ginsenoisdes of the cultured ginsengs by HPLC. After oral administration of the MeOH extract to hyperlipidemic rats for four weeks, the variables including body weight, cholesterol, HDL, LDL, and triglyceride levels in serum were measured. One of the cultured ginseng roots (CBN3) decreased cholesterol and LDL-cholesterol and increased HDL-cholesterol levels in serum. HPLC analysis demonstrated that CBN3 contains more $Rb_1$ and Rd ginsenosides than any other cultured ginsengs and cultivated ginseng. These results suggest that CBN3 with high contents of $Rb_1$ and Rd may be useful in lowering blood cholesterol.

• Journal of Plant Biotechnology
• /
• v.43 no.1
• /
• pp.125-131
• /
• 2016

Pueraria lobata is a perennial legume plant that produces a variety of isoflavones, such as puerarin, daidzin and daidzein. These are metabolized to equol via dihydrodaidzein and tetrahydrodaidzein by the bacterial fermentation of natural isoflavone sources in the human intestines. In this study, we described the growth and accumulation of isoflavone in the hairy root of the Korean wild arrowroot according to the culture period, as well as dihydrodaidzein biosynthesis in hairy root extracts fermented with Pediococcus pentosaceus. Daily proliferation was best in DY1 cultured for 1 week. DY1 showed significant differences in daily production of puerarin and daidzin+daidzein, as compared to DJ7; furthermore, both were best in DY1 cultured for 1 week. The hairy root extract was fermented successfully with P. pentosaceus with confirmed production of dihydrodaidzein, an equol precursor formed by biotransformation. The results indicated that the growth of hairy roots and isoflavone accumulation in the hairy roots is best 1 week after culture. These results are expected to contribute to the mass production of hairy root and isoflavones as equol precursors from the Korean wild arrowroot and provide a basis for equol production by biotransformation in vitro.

• Korean Journal of Organic Agriculture
• /
• v.6 no.1
• /
• pp.151-160
• /
• 1997

Azospirillum sp., photosynthetic bacteria(Rhodopseudomonas sp.) and Pseudomonas sp. were separated and screened from soil and soilless culture, and identificated. The antifungal activities against root-infected pathogens and plant growth promoting effects of the cultured solution of the starins(5.0$\times$105 cells/$m\ell$) in the peatmoss compost on the early growth of cucumber and tomato seedling were investigated. Azospirillum sp. and Pseudomonas sp. showed a antifungal activities against Fusarium sp., Pythium sp. and Rhizoctonia sp in thed ranges of 51.0% to 72.0% on potato dextrose agar medium, however photosynthetic bacteria had not antifungal activities. When cultured solution of Azospirillum sp., photosynthetic bacterial and Pseudomonas sp. were bacterialized by mixing with peatmoss compost, early growth of cucumber and tomato in terms of plant height, number of leaves, leaf area, root length, fresh anf dry weight of leaf, stem and root were promoted, especially photosynthetic bacteria had a the best plant growth promting activities.

• Journal of Life Science
• /
• v.15 no.4 s.71
• /
• pp.673-677
• /
• 2005

This experiment was conducted to clarify the effect of pyroligneous liquor on endogenous substances that were GA (gibberelic acid)-like substances, starch, and protein of Neofinetia falcata cultured in vitro. When seedlings of Neofinetia falcata were treated with several concentrations (0, 0.5, 1.0, 2.0, 3.0 ml/L) of pyroligneous liquor, the growth of seedlings was enhanced in 1.0ml/L pyroligneous liquor added media. The activity of GA-like substances was lower in the leaf, but higher in the and root than that of the control. The content of starch in 1.0ml/L pyroligneous liquor was lower in the leaf, but higher in the root than that of the control. And the content of protein in 1.0ml/L pyroligneous liquor was also lower in the leaf, but higher in the root than that of the control.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.37 no.12
• /
• pp.1654-1659
• /
• 2008

The aim of this study was to compare the fermentation characteristics of tissue cultured mountain ginseng, extruded tissue cultured mountain ginseng, and root hair of red ginseng. Also, pH, acidity, brix, reducing sugar, total sugar, and alcohol were analyzed. The extrusion conditions were barrel temperature of 110 and $140^{\circ}C$ and moisture content of 25 and 35%. Fermentation temperature was $27^{\circ}C$ for 15 days and the cultivation was fixed with Saccharomyces cerevisiae, Aspergillus usamii, and Rhizopus japonicus. The results showed that pH, brix, reducing sugar content, and total sugar content of fermented broths were decreased after 5 days and then maintained steadily for the following 10 days. Acidity of final fermented broths were 1.12% (root hair of red ginseng), 1.19% (tissue cultured mountain ginseng), and $0.97{\sim}1.02%$ (extruded tissue cultured mountain ginseng), respectively. Alcohol content of final fermented broths were 3.82% (root hair of red ginseng), 0.91% (tissue cultured mountain ginseng), and $1.86{\sim}2.18%$ (extruded tissue cultured mountain ginseng). The fermentation efficiency of extruded tissue cultured mountain ginseng (barrel temperature $140^{\circ}C$, moisture content 25%) were the highest. In conclusion, the fermentation efficiency was increased by extrusion process.

• YAKHAK HOEJI
• /
• v.27 no.2
• /
• pp.109-116
• /
• 1983

The effect of protein constituents of six-year old fresh Panax ginseng root on chick embryonic brain, spinal cord and skeletal muscle dissociation cultures was studied. The protein constituents showed the enhancing effect on cultured brain, spinal cord and skeletal muscle cells. The neurite formation from brain and spinal cord cells and the outgrowth of neurite seemed to be enhanced by almost all of the protein constituents employed for this study. The maturation of skeletal muscle cells was stimulated by the protein constituents. This enhancing effect of the protein constituents was more vivid when brain, spinal cord and skeletal muscle cells were cultured with a medium which did not contain chick embryonic extracts known as an essential component for primary cell culture. The protein fraction having molecular weight range of 1,000 to 5,000 out of all the protein fractions employed for this study showed the most stimulatory effect on cultured brain, spinal cord and skeletal muscle cells.