• Title/Summary/Keyword: Culture-negative

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A Comparision of the Sensitivities of Culture, Chlamydiazyme and Direct Immunofluorescence Staining for Chlamydia trachomatis in Non-gonococcal Urethritis (비임균성 뇨도염 환자에서 Chlamydia trachomatis 검출방법에 관한 연구 (배양법, 효소면역법 및 직접면역형광법의 비교 관찰))

  • Choi, Tae-Yeal;Kim, Choon-Won;Kim, Jung-Hwan
    • The Journal of the Korean Society for Microbiology
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    • v.21 no.3
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    • pp.393-397
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    • 1986
  • Chlamydia trachomatis is one of the most common cause in non-gonococcal urethritis. There are several diagnostic methods for Chlamydia trachomatis; culture method using McCoy cell, enzyme immunoassay and direct immunofluorescence staining etc. We have studied a sensitivities of culture, chlamydiazyme and direct immunofluorescence staining(DIF). 85 male patients previously conformed to non-gonococcal urethritis have been selected in this study. Three samples were concurrently collected in the same patient. First sample was used to inoculation in McCoy cell, 2'nd sample was used to Chlamydiazyme test and 3'rd sample was used to direct immunofluorescence staining method. The results are following. 1. All culture, Chlamydiazyme and DIF positive cases are 15/85(17.7%). 2. Culture and Chlamydiazyme positive but DIF negative cases absent. 3. Culture and DIF positive, but Chlamydiazyme negative cases are 2/85(2.4%). 4. Chlamydiazyme and DIF positive, but culture negative cases are 9/85(10.6%). 5. Culture positive, but Chlamydiazyme and DIF negative cases are 6/85(7.1%). 6. Chlamydiazyme positive, but culture and DIF negative cases are 7/85(8.2%). 7. DIF positive, but culture and Chlamydiazyme negative cases are 3/85(3.5%). 8. All culture, Chlamydiazyme and DIF negative cases are 43/85(50.1%). In summarized, anyone positive cases of culture, Chlamydiazymc and DIF are 42/85(49.9%).

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Results of Culture Test at the Time of Removal of Metal Implants Used for Ankle Fracture Management (족관절 골절 치료에 사용한 금속 내고정물 제거 수술 시 시행한 균 배양 검사의 결과)

  • Chung, Hyung-Jin;Bae, Su-Young;Yu, Jae-Ha
    • Journal of Korean Foot and Ankle Society
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    • v.18 no.2
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    • pp.68-71
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    • 2014
  • Purpose: The purpose of this study is to report the results of culture test at the time of removal of metal devices used for management of ankle fractures and for analysis of contributing factors. Materials and Methods: We reviewed medical records of 132 patients with lower tibia and ankle fracture who had their metal devices removed during the period from January 2010 to February 2014. Patients with clinical signs of infection were excluded. Culture test was performed by taking the granulation tissue around the metal device at the time of removal. We divided the subjects into two groups, culture positive and negative. We then performed a retrospective review of each medical record of multiple factors that might contribute to the culture results, including laboratory results, medical history, material and size of metal device, indwelling period, and whether or not it was open injury. Results: Among 132 cases, six were culture positive. Enterococcus was detected in two cases and the others were Staphylococcus. No significant difference in medical history of patients and laboratory results, including C-reactive protein level, was observed between the culture positive and negative group. Culture positive rate was 5.4% in titanium and 3.9% in stainless steel. In terms of metal size, culture positive rate was 5.1% in small plates, 6.7% in large plates, and culture negative in intramedullary nails. The average indwelling period of metal device was 61.5 weeks in the culture positive group, and 68.6 weeks in the negative group. Nine cases were open fractures and all were in the culture negative group. Conclusion: Whether or not the culture result was positive, there were no meaningful contributing factors. Presence of bacterium on the metal device could not be screened by any laboratory results or other factors.

The Effect of Job Stress and Job Exhaustion on Job Performance of Body Guards and Security Guards due to Organizational Culture

  • Kim, Sang-Jin
    • Journal of the Korea Society of Computer and Information
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    • v.25 no.7
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    • pp.193-202
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    • 2020
  • This study conducted a questionnaire survey of 320 security guards to investigate the effects of job stress and job exhaustion on job performance according to the bodyguard's organizational culture. The conclusions obtained through this study are as follows. First, in the organizational culture, the innovation culture was found to have a negative effect on the job stress at the .01 level. Second, organizational culture was found to have no direct effect on job performance. Third, among organizational cultures, market culture was found to have a statistically negative effect on job performance at the .01 level. Fourth, job stress was found to affect job performance statistically as a negative (-) at .001 level. Fifth, job exhaustion was found to affect job performance statistically as negative (-) at .001 level. Sixth, job stress was found to have a mediating effect as negative (-) at the .01 level statistically in relation to the innovation culture of organizational culture and job performance. Seventh, job exhaustion was found to have no statistically significant effect on the relationship between organizational culture and job performance.

Prediction of pathogen positive-culture results in acute poisoning patients with suspected aspiration (흡인이 의심되는 급성 중독환자에서 병원균 양성 배양 결과의 예측)

  • Baek Sungha;Park Sungwook
    • Journal of The Korean Society of Clinical Toxicology
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    • v.20 no.2
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    • pp.75-81
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    • 2022
  • Purpose: This study sought to compare the characteristics of patients with pathogen-positive and negative cultures, and to investigate factors predicting pathogen-positive culture results in patients of acute poisoning with suspected aspiration. Methods: Consecutive patients with acute poisoning admitted to an intensive care unit between January 2016 and December 2018 were retrospectively studied. Respiratory specimens were collected from the enrolled patients at the time of the suspected aspiration. We compared the characteristics of patients with pathogen-positive and negative culture results and analyzed the causative pathogens. Results: Among the 526 patients, 325 showed no clinical features that could be attributed to aspiration, and 201 patients had clinical features suggestive of aspiration. Of these, 113 patients had pathogen-positive culture, 61 were negative, and the specimens of 27 patients contained poor-quality sputum. In univariate analysis, patients with a positive culture showed a longer time to culture from ingestion (p=0.01), faster heart rate (p=0.01), and higher partial pressure of arterial oxygen to the fraction of inspired oxygen (PaO2/FiO2) (p=0.02) than patients with negative culture. Multivariate analysis demonstrated that PaO2/FiO2 (adjusted odd ratio, 1.005; 95% confidence interval [CI], 1.002-1.008; p=0.005) was a significant risk factor for pathogen-positive culture. The area under the receiver operating characteristic curve of PaO2/FiO2 was 0.591 (95% CI, 0.510-0.669, p=0.05). Gram-negative pathogens (GNPs) were predominant and at least one GNP was observed in 84 (73.3%) patients among those with pathogen positive culture. Conclusion: We failed to find any clinical factors associated with positive culture results. Antibiotics that cover GNPs could be considered when deciding the initial antibiotic regimen at the time of suspected aspiration.

A Case Study on Adaptability Factors in Family Life for Vietname Multi-cultural Families in Korea in Terms of the Inter-culturalism (문화상호주의적 관점에서 본 베트남 다문화가족의 가족생활적응 사례연구)

  • Shin, Yoo-Kyung;Chang, Jin-Kyung
    • Journal of Family Resource Management and Policy Review
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    • v.14 no.3
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    • pp.109-122
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    • 2010
  • The present study aims to investigate cultural similarities and differences that influence interracial couples (between Korean men and Vietnamese women) when adapting to Korean family culture in terms of interculturalism. The interviewees consisted of three generations. This study used in-depth interviews. Data was collected from January to August 2008. 15 families (45 people) were used for analysis. Results were as follows: (1) the patriarchal culture of the two nations had a positive influence on the adaptation of members during family interactions(2) the culture of filial piety is a value system that both nations believe is important. However, different rituals had a negative influence on the practice of filial piety (3) the community-oriented culture is regarded as important in the two nations. However, Vietnamese wives cannot lead a free community life because Koreans see them in negative terms (4) the Vietnamese traditional wedding custom called "NopJjeOh" had a negative influence on the adaptation to the Korean family culture (5) the preference for sons in Vietnam is not as high as in Korea. This difference had a negative influence on adaptation (6) clear differences between the daily lives of the two nations had a negative influence on adaptation to Korean family culture. In conclusion, Korea and Vietnam have many similarities in culture, which enable Vietnamese wives to adapt to Korean culture more easily than other foreign wives. If various programs are developed to promote the mutual understanding between both cultures based on these research findings, it would further contribute toward social integration in Korea.

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Cultural Backgrounds of Reviewers, Negative Emotions, and Review Helpulness: Based on Impression Management Theory (리뷰어의 문화적 배경, 부정적 감정표현, 그리고 리뷰유용성: 인상관리 이론을 중심으로)

  • Jungwon Lee;Ohsung Kim;Cheol Park
    • Information Systems Review
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    • v.26 no.2
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    • pp.59-81
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    • 2024
  • Online consumer reviews provide a variety of information from the customer perspective in terms of satisfaction and dissatisfaction. Negative emotional expression is a potential antecedent of review usefulness, and can also influence potential consumers' attitudes and decisions. In addition, because national culture provides a perspective from which individuals view the world and act, it is highly likely that differences in negative emotional expression will occur depending on culture. This study explores the relationship between national culture and negative emotional expression based on impression management theory and ultimately analyzes the impact on review usefulness. For empirical analysis, 16,076 reviews of 140 hotels located in Seoul were collected and analyzed using the PLS-SEM method. As a result of the analysis, it was found that power distance and masculinity culture dimensions had a positive effect on reviewers' negative emotional expressions, while uncertainty avoidance and long-term orientation had a negative effect. In addition, negative emotional expression was analyzed to have a positive effect on review usefulness even when review ratings were controlled.

The Relation of Organization Structure, Organization Culture and Organization Conflict of University Hospital Staff Members (대학병원 종사자의 조직구조, 조직문화에 따른 조직갈등의 관계에 대한연구)

  • Lee, Chang-Ho;Cho, Duk-Young;Kim, Yun-Jin
    • The Korean Journal of Health Service Management
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    • v.9 no.1
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    • pp.95-107
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    • 2015
  • The purpose of this study is to analyze the characteristics of hospital organization structure, organization culture, organization conflict and the interrelation of influential factor in organization conflict. The data for this study were collected through a self-administered survey with a structured delivered to 755 subjects including several nursing staff members, administration staff members and medical technicians at six hospitals. The structural equation model was used to examine the relation of organization structure, organization culture and organization conflict. The results of the analysis in this study were as follows. First, organization structure had a positive influence on organization conflict, especially in terms of complexity and centralization. But, formalization had a negative influence on organization conflict. Second, organization culture had a positive influence on organization conflict, especially in terms of group culture, developmental culture, and hierarchical culture. But, group culture had a negative influence on organization conflict. Third, organization structure had positive influences, especially on organization culture complexity, centralization, and formalization. But, centralization had negative influences too.

Exploring Impact of Positive/Negative Valence Order on Repeated Exposure to Suspenseful Stories

  • Chang Ui Chun
    • International Journal of Advanced Culture Technology
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    • v.11 no.3
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    • pp.182-189
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    • 2023
  • This study investigates the psychological effects of positive/negative valence order in repeated exposure to a suspenseful text. Specifically, the study seeks to understand how the order in which positive and negative elements are presented in a narrative impacts the experience of suspense, arousal, and enjoyment in readers or listeners. Using a suspenseful short story, participants were exposed to narratives with varying valence orders in a repeated-measures design. The study employed self-report questionnaires and psychophysiological measurements to capture participants' psychological responses. The results supported the hypothesis that repeated exposure impacts suspense, with negative valence enhancing suspense and arousal. Moreover, the order of valence influenced participants' psychological responses, indicating that positive valence can mitigate the impact of repeated exposure. However, the influence on enjoyment was not significant. Psychophysiological measures, specifically skin conductance level (SCL), revealed trends of habituation over repeated exposure. The findings underscore the significance of negative valence in heightening suspense and suggest directions for future research in exploring diverse factors that contribute to suspense in both fictional and real-life contexts.

Evaluation of Negative Results of BacT/Alert 3D Automated Blood Culture System

  • Kocoglu M. Esra;Bayram Aysen;Balcl Iclal
    • Journal of Microbiology
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    • v.43 no.3
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    • pp.257-259
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    • 2005
  • Although automated continuous-monitoring blood culture systems are both rapid and sensitive, false-positive and false-negative results still occur. The objective of this study, then, was to evaluate negative results occurring with BacT/Alert 3D blood culture systems. A total of 1032 samples were cultured with the BacT/Alert 3D automated blood culture system, using both aerobic (BPA) and anaerobic (BPN) media, and 128 of these samples yielded positive results. A total of 904 negative blood samples were then subcultured in $5\%$ sheep blood agar, eosin methylene blue, chocolate agar, and sabouraud-dextrose agar. Organisms growing on these subcultures were subsequently identified using both Vitek32 (bioMerieux, Durham, NC) and conventional methods. Twenty four $(2.6\%)$ of the 904 subcultures grew on the subculture media. The majority $(83.3\%)$ of these were determined to be gram-positive microorganisms. Fourteen $(58.3\%)$ were coagulase-negative staphylococci, two $(8.3\%)$ were Bacillus spp., one $(4.2\%)$ was Staphylococcus aureus, and one $(4.2\%)$ was identified as Enterococcus faecium. Streptococcus pneumoniae and Neisseria spp. were isolated together in two $(8.3\%)$ vials. Gram-negative microorganisms comprised $12.5\%$ of the subcultures, of which two $(8.3\%)$ were found to be Pseudomonas aeruginosa, and one $(4.2\%)$ was Pseudomonas fluorescens. The other isolate $(4.2\%)$ was identified as Candida albicans. We conclude that the subculture of negative results is valuable in the BacT/Alert 3D system, especially in situations in which only one set of blood cultures is taken.

Relationship among Types of Nursing Organizational Culture, Self-leadership and Burnout as Perceived by Perioperative Nurses (수술실 간호사가 지각하는 간호조직문화 유형, 셀프리더십 및 소진 간의 관계)

  • Im, Minkyung;Sung, Young-Hee;Jung, Junghee
    • Journal of Korean Academy of Nursing Administration
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    • v.23 no.2
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    • pp.170-180
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    • 2017
  • Purpose: This study was designed to assess the types of nursing organizational culture, and degree of self-leadership and burnout as perceived by perioperative nurses, and to identify correlations between these variables. Methods: Participants were 155 nurses from 3 tertiary hospitals in Seoul and data were analyzed using descriptive statistics, ANOVA, Scheffe, and Pearson correlation coefficient. Results: In the types of nursing organizational culture, the mean score for hierarchy-oriented culture was 3.57, and for self-leadership, 3.61 and for burnout, 2.87. In the relationship between the types of nursing organizational culture, self-leadership and burnout, relation-oriented culture (r=.24, p<.01), innovation-oriented culture (r=.23, p<.01) and task-oriented culture (r=.22, p<.01) had a slight positive correlation with self-leadership in that order. The innovation-oriented culture (r=-.29, p<.01) and relation-oriented culture (r=-.42, p<.01) among the types of nursing organizational culture showed a negative correlation with burnout while the hierarchy-oriented culture (r=.28, p<.01) showed a positive correlation with burnout. Self-leadership showed a negative correlation with burnout (r=-.42, p<.01). Conclusion: The results show that nurses in operating rooms have a high awareness of hierarchy-oriented culture that affects burnout in a negative way while having a low awareness of relation-oriented culture that has a positive influence on burnout.