• 중환자간호학회지
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• 제16권1호
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• pp.15-27
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• 2023

Purpose : The purpose of this study is to identify the factors affecting the degree of job embeddedness by examining role conflict among intensive care unit nurses, the nursing organizational culture, nurse-physician collaboration, degree of job embeddedness, to understand the relationship between these variables. Method : Participants are 148 nurses from two hospitals in the intensive care unit. Collected data are analyzed using independent t-tests, ANOVA, Scheffé test, Pearson correlations, and multiple regressions using the SPSS 25.0 program. Results : The factors that significantly influenced the participants' job embeddedness are role conflict (𝛽=-.19, p =.015), innovation-oriented culture (𝛽=.26, p =.003), and nurse-physician collaboration (𝛽=.24, p =.002). The total explanatory power of these factors for job embeddedness is 44.5% (F=15.06, p =.001). Conclusion : This study identifies role conflict among intensive care unit nurses, innovation-oriented culture, and nurse-physician collaboration as important factors affecting job embeddedness.

• 한국환경보건학회지
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• 제23권4호
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• pp.73-81
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• 1997

Diphtheria was the world-spread, respiratory infectious disease in the past. But after developing the diphtheria toxoid, diphtheria vaccine, composed of diphtheria toxoid, is generalized, so prevalence of diphtheria is sharply dropped. But diphtheria is come back in NIS(New Independent States) in 1990s. It is believed that recurrence of diphtheria is mainly due to shortage of protective diphtheria antibody titer in adults. And it is related to 70% prevalence in adults, not in children. Considering this changing environment, this study was designed to research the diphtheria antibody titer of whole population and existence of titer difference by sex. Also it was studied whether ELISA has fitness as determination method of diphtheria antibody titer compared with neutralization method on microcell culture. This study was done to 277 samples of three hospitals in Seoul area, and sera was tested by neutralization method on microcell culture and ELISA method. The results of this study were as follows 1) Of this studied population, 31.05% had an antibody titer below the protective level (<0.01 IU/ml), 40.79% had a titer of a relative degree of protection (0.01-0.09 IU/ml) and 28.16% had a reliable degree of protection ($\geq$0.1 IU/ml). Therefore, 68.95% had a basic protective antibody titer level. 2) 20-40 age group showed the lowest diphtheria antibody titer among the studied population. 3) GMT of diphtheria antibody titer was the highest in the children. But, after child period, GMT was fallen. Standard deviation value was the lowest in older group. 4) Protective level of diphtheria antibody titer of male was slightly higher than female (70.94% vs 66.66%). But this difference was not statistically significant. 5) Diphtheria antibody titer by ELISA method was wholly higher than by neutralization method on microcell culture. Compared the two results by ELISA and neutralization, the regression coefficient was 0.38. And in titer, which was obtained by ELISA method, false-positive results was abundant.

• Journal of Microbiology and Biotechnology
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• 제15권1호
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• pp.66-71
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• 2005

Doxorubicin is a clinically important anticancer polyketide compound that is typically produced by Streptomyces peucetius var. caesius. To improve doxorubicin productivity by S. peucetius, a doxorubicin pathway-specific regulatory gene, dnrI, was cloned into a high-copy-number plasmid containing a catechol promoter system. The S. peucetius containing the recombinant plasmid exhibited approximately 9.5-fold higher doxorubicin productivity compared with the wild-type S. peucetius. The doxorubicin productivity by this recombinant S. peucetius strain was further improved through the optimization of culture media composition. Based on the Fractional Factorial Design (FFD), cornstarch, $K_2HPO_4$, and $MgSO_4$ were identified to be the key factors influencing doxorubicin productivity. The Response Surface Method (RSM) results based on 20 independent culture conditions with varying amounts of key factors predicted the highest theoretical doxorubicin productivity of 11.1 mg/l with corn starch of 46.33 g/l, $K_2HPO_4$ of 4.63 g/l, and $MgSO_4$ of 9.26 g/l. The doxorubicin productivity of the recombinant S. peucetius strain with the RSM-based optimized culture condition was experimentally verified to be 11.46 mg/l, which was approximately 30.8-fold higher productivity compared with the wild-type S. peucetius without culture media optimization.

• 한국식생활문화학회지
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• 제34권2호
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• pp.93-111
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• 2019

This paper provides a comprehensive description of previous studies of making and developing kimchi in terms of the origins of kimchi and the changes in the history of kimchi, along with newly discovered data, including the collection of works in Joseon Dynasty as well as old cookbooks discovered after the year 2000, and latest research in related disciplines, e.g., ancient history studies, archeology, and linguistics. Because new ruins and relics, such as the Liao-ho Civilization in the Northeast Asia continent, which was closely related to Dongyi tribes, have been discovered and studied, it is important to determine how to reflect the outcome of archeological studies on the origin of preserved vegetables. In addition, to describe the background and changes in the independent formation of making Korean kimchi chronologically, they were divided into the following: development from jjanji to singunji; formation of the basis for seokbakji dressed with fermented fish sauce; settlement of seokbakji culture for dressing seokbakji with fermented fish sauce and spices, including chili varieties when they were introduced; and establishment of the method for making whole cabbage kimchi, which is currently typical kimchi made by adding the seokbakji as the stuffing of the cabbage kimchi, to examine the time and specific details of the change.

• International Journal of Advanced Culture Technology
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• 제5권1호
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• pp.76-82
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• 2017

An image hash, a discriminative and robust summary of an image, should be robust against quality-preserving signal processing steps, while being pairwise independent for perceptually different inputs. In order to improve the hash matching performance, this paper proposes a probabilistic dissimilarity matching. Instead of extracting the binary hash from the query image, we compute the probability that the intermediate hash vector of the query image belongs to each quantization bin, which is referred to as soft quantization binning. The probability is used as a weight in comparing the binary hash of the query with that stored in a database. A performance evaluation over sets of image distortions shows that the proposed probabilistic matching method effectively improves the hash matching performance as compared with the conventional Hamming distance.

• 수완나부미
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• 제13권1호
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• pp.65-87
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• 2021

This study attempted to investigate the effects of collectivism as an individual cultural value and leadership practices on employees' organizational commitment of six commercial banks in Vietnam. The study used collectivism and the implementation of five leadership practices as the independent variables, the three components of organizational commitment as the dependent variables and employees' personal factors served as controlling variables. The study utilized a quantitative method of research with questionnaires as the main data collection instrument. The findings of the study have revealed that the individual cultural value of collectivism positively, but to a marginal degree, affected the level of employees' organizational commitment. Among five leadership practices, only the implementation of encourage the heart, positively, but to a marginal degree, influenced employees' affective and normative commitment. The leadership practice of challenge the process had a negative impact on the employees' affective commitment. The personal factors of employees did not have an impact on organizational commitment. The findings and conclusions of this study may be used by the leaders to create the favorable working environment, improve the relationship between leaders and followers and to increase organizational commitment.

• International Journal of Advanced Culture Technology
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• 제10권3호
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• pp.393-396
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• 2022

Recently, rapid climate change has had a significant impact on the ecosystem of honeybees. In addition, the problem of Vespa Hornets invasion of colonies has a fatal impact on the bee ecosystem, independent of climate change. Especially in late summer. This study relates to a method for preventing Vespa Hornets attack. In this study, we developed a Vespa Hornets sound detection device was developed by collecting and analyzing the sound of a Vespa Hornets and applying IoT technology. The developed device detects the sound of a Vespa Hornets when Vespa Hornets appears around the hive of the bees and sends a signal to automatically close the door of the beehive. The device that receives the signal drives the motor that controls the honeycomb door to close the beehive door. The Vespa Hornets sound detection device operates until no Vespa Hornets sound is detected. The system developed by us is expected to be installed in the beehives of actual beekeeping farms to dramatically reduce the damage caused by by Vespa Hornets.

• 비교교육연구
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• 제27권1호
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• pp.171-197
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• 2017

중국의 독립학원은 민간의 교육투자를 인정하여 투자자 개인이나 기업, 사회의 지원 자금 활용을 용이하게 하고, 학교의 학사 운영, 학생 입학전형, 예산의 집행 및 관리, 별도의 법인 설립 및 학력 인정 등에 대해서 국가 주도로 설립된 공립대학과 다르게 사립대학인 민판학교 방식으로 자율적인 운영을 보장하는 등 중국내의 고등교육기관의 다양성을 갖추고자 하는 목적으로 설립되었다. 하지만 1990년대 초반 이후 중국 고등교육의 새로운 방식으로서 나타나게 된 독립학원은 지난 30여년의 시간 동안 양적인 성장을 이루었음에도 아직 교육의 질적 측면에서의 성장은 미흡한 상황이다. 중국의 독립학원이 관심을 받는 이유는, 독립학원이 모체 대학(母體)의 시설과 교수, 개설된 전공에서 지원을 받고 대학의 명성을 공유하는 등 대학 자체의 발전을 위한 노력보다는 학생 모집에 중점을 두는 방식으로 운영한다는 것이다. 따라서 본 연구는 중국의 독특한 고등교육기관인 독립학원의 현황과 관련한 정책을 분석하고자 하였다. 연구 목적을 달성하기 위해 다차원 교육정책분석 모형을 활용하여, 규범적 차원으로 중국의 독립학원 정책의 영리성 문제와 중국인의 차서격국(差序格局) 중심의 ?시(關係) 문화를, 구조적 차원으로 독립학원 관련 정부기관 구조, 국유민판의 운영구조를, 구성적 차원으로 중국의 독립학원 이해관계자의 속성을, 기술적 차원으로 독립학원 정책과 법제화 과정을 살펴보고자 하였다. 분석 결과를 통해 중국의 독립학원 정책의 방향성에 대한 시사점을 제시하고자 한다.

• Journal of Microbiology
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• 제42권1호
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• pp.9-13
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• 2004

Culture-independent approaches, based on 16S rDNA sequences, are extensively used in modern microbial ecology. Sequencing of the clone library generated from environmental DNA has advantages over fingerprint-based methods, such as denaturing gradient gel electrophoresis, as it provides precise identification and quantification of the phylotypes present in samples. However, to date, no method exists for comparing multiple bacterial community structures using clone library sequences. In this study, an automated method to achieve this has been developed, by applying pair wise alignment, hierarchical clustering and principle component analysis. The method has been demonstrated to be successful in comparing samples from various environments. The program, named CommCluster, was written in JAVA, and is now freely available, at http://chunlab.snu.ac.kr/commcluster/.

• Journal of Microbiology
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• 제44권2호
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• pp.155-161
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• 2006

Comparative analysis of microbial communities in a sequencing batch reactor which performed enhanced biological phosphorus removal (EBPR) was carried out using a cultivation-based technique and 16S rRNA gene clone libraries. A standard PCR protocol and a modified PCR protocol with low PCR cycle was applied to the two clone libraries of the 16S rRNA gene sequences obtained from EBPR sludge, respectively, and the resulting 424 clones were analyzed using restriction fragment length polymorphisms (RFLPs) on 16S rRNA gene inserts. Comparison of two clone libraries showed that the modified PCR protocol decreased the incidence of distinct fragment patterns from about 63 % (137 of 217) in the standard PCR method to about 34 % (70 of 207) under the modified protocol, suggesting that just a low level of PCR cycling (5 cycles after 15 cycles) can significantly reduce the formation of chimeric DNA in the final PCR products. Phylogenetic analysis of 81 groups with distinct RFLP patterns that were obtained using the modified PCR method revealed that the clones were affiliated with at least 11 phyla or classes of the domain Bacteria. However, the analyses of 327 colonies, which were grouped into just 41 distinct types by RFLP analysis, showed that they could be classified into five major bacterial lineages: ${\alpha},\;{\beta},\;{\gamma}-$ Proteobacteria, Actinobacteria, and the phylum Bacteroidetes, which indicated that the microbial community yielded from the cultivation-based method was still much simpler than that yielded from the PCR-based molecular method. In this study, the discrepancy observed between the communities obtained from PCR-based and cultivation-based methods seems to result from low culturabilities of bacteria or PCR bias even though modified culture and PCR methods were used. Therefore, continuous development of PCR protocol and cultivation techniques is needed to reduce this discrepancy.