• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.39-39
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• 2005

Large scale mammalian cell culture has become, over the past two decades, the preferred method to produce therapeutic monoclonal antibodies. In this presentation, I will introduce disposable bioreactor system and analyze key factors and points for consideration during mammalian cell culture process development. Example will be provided highlighting the selection of master cell, culture media and environmental factors based on productivity and product quality.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.7 no.1
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• pp.161-165
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• 1969

Since Guha and Maheshwari's works on the induction of haploid plant from the cultured anther of Datura innoxia an her culture has become a big topic among geneticists and breeders. Presen paper is the summarized report on the anther culture method based on the author's recent researches.

• Food Science and Biotechnology
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• v.17 no.4
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• pp.892-894
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• 2008

During dongchimi fermentation at 5 and $25^{\circ}C$, the pH lowered slowly and reached 4.03 at $5^{\circ}C$ after 30 days, whereas it lowered dramatically and reached 3.59 at $25^{\circ}C$ after 2 days. The predominant bacteria were Leuconostoc (Leu.) mesenteroides at $25^{\circ}C$ until day 2 which changed into Lactobacillus (Lb.) plantarum at day 3, analyzed by a culture dependent method with partial 16S rRNA gene sequencing, whereas Leu. mesenteroides occupied predominantly at $5^{\circ}C$ until day 7. In a culture-independent method using a polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) with partial 16S rRNA gene sequencing, Lb. algidus was predominant at $5^{\circ}C$ until day 7 and Lb. plantarum occupied predominantly at $25^{\circ}C$ until day 3, which is different from the results of the culture based method, indicating the both methods need to be combined for accuracy. Based on the culture-dependent method, Leu. mesenteroides might be responsible for the early and mid-phase of dongchimi fermentation.

• Management & Information Systems Review
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• v.1
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• pp.133-165
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• 1997

In the situation of Korean company today it is considered that the necessity of developing a corporate culture is not only more important than ever, but also indispensable to the organization management in the future. Focused on the development of corporate culture, the purpose of this study is to find out the influence of the direction of corporate culture development on its own strength. The method of this study is the empirical method based on theoretical one with the previous bibliographical studies. It is thought that in viewpoints of the culture pragmatists, the development of corporate culture means the intentional and systematic creation of culture. And, the direction of corporate culture development is thought as the management philosophy a corporate desires, or the desirous direction of corporate culture to express the management policy. According to the results of this study, it is found that the progressive direction of the corporate culture development has the most intensive influence on the various sides. Therefore, the management should research and develop the progressive value of culture more deeply, to get the effective development of corporate culture.

• The Research Journal of the Costume Culture
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• v.5 no.1
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• pp.151-164
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• 1997

This study pursues the problems of plan photogrammetry which is widely used in somatotyping at present, and find out a method which can improve accuracy of measurement on the basis of principles and mechanisms of photography-the basic foundation of the photographic analysis methods. As a result, this study proposes a new method which is based on the reference point method and perspective coordinate system. And the test measurement was operated to compare the measurement accuracy of the proposed method and the method based on reference grid screen method and perpendicular coordinate system which is commonly used at present. The result of this test measurement showed that the proposed method has higher accuracy. Two reasons can be pointed out for the improvement of measuring accuracy. The first reason is that the proposed perspective coordinate system reduces the perspective distortion of photography. And second reason is that measuring points can be closely placed to the scale and coordinate reference plan of measurement by the proposed reference point method which make possible to place measuring object (or person) at the center of scale and coordinate reference plan by utilizing reference points of measurement in the three dimensional space not on screen.

• Korean Institute of Interior Design Journal
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• no.13
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• pp.102-110
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• 1997

We have lost our true characteristics without being grown and development of our traditional beauty in state of drawing into a whirlpool as the environment of western cultrue. So, to be existed for self-sown capability of our traditional culture, we'll have to keep up with the unique and creative Korean traditional furniture design through the synchloization between 'Traditionalism in our already westernized life' and 'Korean identity in our already westernized life' and 'Korean identity in worldwide culture' based on independent position by correct view of worth, as for the escape from attitude of receive on western culture in recklessnese. Hereupon, first of all, after reviewof fornuclation backround and characteristics in traditional furniture to be established by an ancestor, based on this and extract the conceptual language, it was presented past published works as for the approaching method toward physical form aspect by use of pattern of modern access method. Secondly, it was the symbolic approaching from method based on traditional and cultural context. Gradually, the embodiment of traditional image, in today's features of our furniture loosing the particular cultural identify is very important aspect, and beside above mentioned two methods, it should be investigated as for the diversified approaching study. Thereafter, we'll have to establish the positionof our modern furniture in global wordwide, for seek the beauty being interrelated form past historical communications, under the circumstance of original and future-oriented value.

• International Journal of Advanced Culture Technology
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• v.1 no.2
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• pp.1-6
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• 2013

In this paper, we evaluate performance of existing similarity measurement metric and propose a novel method using user's preferences information entropy to reduce MAE in memory-based collaborative recommender systems. The proposed method applies a similarity of individual inclination to traditional similarity measurement methods. We experiment on various similarity metrics under different conditions, which include an amount of data and significance weighting from n/10 to n/60, to verify the proposed method. As a result, we confirm the proposed method is robust and efficient from the viewpoint of a sparse data set, applying existing various similarity measurement methods and Significance Weighting.

• International Journal of Advanced Culture Technology
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• v.10 no.4
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• pp.361-368
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• 2022

This study seeks to determine the research trend of late adolescents by utilizing big data. Also, seek for research trends related to activity participation, treatment, and mediation to provide academic implications. For this process, gathered 1.000 academic papers and used TF-IDF analysis method, and the topic modeling based on co-occurrence word network analysis method LDA (Latent Dirichlet Allocation) to analyze. In conclusion this study conducted analysis of activity participation, treatment, and mediation of late adolescents by TF-IDF analysis method, co-occurrence word network analysis method, and topic modeling analysis based on LDA(Latent Dirichlet Allocation). The results were proposed through visualization, and carries significance as this study analyzed activity, treatment, mediation factors of late adolescents, and provides new analysis methods to figure out the basic materials of activity participation trends, treatment, and mediation of late adolescents.

• International Journal of Internet, Broadcasting and Communication
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• v.7 no.1
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• pp.36-41
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• 2015

This paper is proposed a wavelet-based MCDT(Mask Coefficient Differential and Threshold) method of image registration of Multi-images contaminated with visible image and infrared image. The method for ensure reliability of the image registration is to the increase statistical corelation as getting the common feature points between two images. The method of threshold the wavelet coefficients using derivatives of the wavelet coefficients of the detail subbands was proposed to effectively registration images with distortion. And it can define that the edge map. Particularly, in order to increase statistical corelation the method of the normalized mutual information. as similarity measure common feature between two images was selected. The proposed method is totally verified by comparing with the several other multi-image and the proposed image registration.

• International Journal of Advanced Culture Technology
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• v.7 no.2
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• pp.34-41
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• 2019

The purpose of this study was to examine the meaning and structure of the experience of female college students. For this purpose, 22 female college students, regardless of age, participated in the interview three times in total. Interview data were processed through the analysis and interpretation process using the phenomenological research method, Giorgi method. As a result, 34 semantic units were derived, then divided into 14 subcomponents, and then divided into 6 categories. As a result of analysis, the spiritual value of female college students was composed of "family", "friendly person", "professional person", "empathy", "reflection" and "trust". Based on the above meaning, the structure of the spiritual value of female college students can conclude that they were a continuation of life that forms a strong sense of value and empathy and trust with patience with family and friends. Based on this, intervention on spiritual well-being of female college students suggests that intervention to form values based on empathy and trust based on family and close friends is implemented.