• Title/Summary/Keyword: Culture solution

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Growth, Nutrient Status and Net Photosynthetic Rate of Pinus densiflora Seedlings in Various Levels of Aluminum Concentrations (알루미늄 농도(濃度)에 따른 소나무 묘목(苗木)의 생장(生長), 영양상태(營養狀態) 및 광합성속도(光合成速度)에 미치는 영향(影響))

  • Lee, Choong Hwa;Jin, Hyun O;Izuta, Takeshi
    • Journal of Korean Society of Forest Science
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    • v.88 no.2
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    • pp.249-254
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    • 1999
  • The effects of various levels of Al concentration on growth, nutrient status and net photosynthetic rate of 2-year-old Pinus densiflora Sieb. et Zucc. seedlings grown in a nutrient culture solution were investigated. Al concentrations were added as aluminum chloride($AlCl_3$) at 0(control), 10, 30 and 60ppm to the nutrient culture solution. The nutrient culture solution was maintained at pH 4.0 by adding HCl or NaOH solution. The seedlings were transplanted into the nutrient culture solution and grown in a greenhouse for 90 days from May 8 to August 6, 1996. The treatment above 10ppm of Al concentrations induced a significant reduction on the dry weight growth of the seedlings. The relative growth rate(RGR), net assimilation rate(NAR) and net photosynthetic rate of the seedlings were reduced with increasing of Al concentrations in the nutrient culture solutions. This result suggests that reductions in the RGR and NAR of the seedlings were mainly due to the inhibition of net photosynthesis. In addition, the increase of Al concentrations in a nutrient culture solution decreased the concentration of essential mineral elements such as Ca and Mg in the needle of the seedlings. However, the concentrations of Al of each plant organ increased in the treatment above 10ppm of Al concentrations in the nutrient culture solutions. This result suggests that the increased Al concentration in the belowground part resulted from the decreased concentration of essential mineral elements in the aboveground part of the seedlings.

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Effect of Chlorella sp. on Improving Antioxidant Activities and Growth Promotion in Organic Soybean Sprout Cultivation (클로렐라 처리에 의한 유기농 콩나물 생육촉진 및 항산화 능력 증진효과)

  • Kim, Min-Jeong;Shim, Chang-Ki;Kim, Yong-Ki;Hong, Sung-Jun;Park, Jong-Ho;Han, Eun-Jung;Jee, Hyeong-Jin;Lee, Sung-Buk;Kim, Seok-Cheol
    • Korean Journal of Organic Agriculture
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    • v.23 no.4
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    • pp.939-950
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    • 2015
  • The purpose of this study was to estimate the growth promoting effects and improvement of antioxidant activity of the soybean sprouts treated with Chlorella sp. culture solution. The soybean sprout treated with 0.1% and 0.2% Chlorella sp. culture solution was significantly increased the length (more than 43.0%), the thickness (more than 0.5~0.7 mm), fresh weight (more than 2.9~3.7 g) compared to non-treated control in vitro. In organic soybean sprouts farm, the 0.2% chlorella culture solution applied to mass culture of soybean sprout and the fresh weight of soybean sprouts increased by more than 25% and the yield was very high as 598.33% compared to untreated control. In addition of sensory test, there is no fishy odor and better crunchy texture and nutty flavor for the treatment soybean sprouts compared to untreated soybean sprouts. Particularly, free-radical scavenging activity (DPPH) and superoxide dismutase activity (SOD) of the soybean sprouts were significantly increased more than 26.1% and 40.4%, respectively by treated with 0.1% and 0.2% Chlorella culture solution. Consequently, the treatment of chlorella culture solution to grow soybean sprouts is also promoting quality and antioxidant activity as well as promoting the growth of sprouts. Therefore, chlorella is considered to be worth as functional materials for high-quality sprouts grown.

Priority Determination in Nutrient Solution Management in Perlite Culture (펄라이트재배에서의 급액관리의 priority 설정)

  • 김영식
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.5 no.1
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    • pp.7-12
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    • 2004
  • The steps related to the nutrient solution management were divided into 4 categories. The resistance for water movement in every steps was proposed. The priorities among the steps were set according to the cultural situation, which were used as the basis for the management in perlite bag culture. The enhancement of historical root development is especially critical, as well as how to supply essential water efficiently by minimizing the water movement distance to the root, which is the narrowest neck of a bottle in solution management.

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Effects of Compost Leachate and Concentrated Slurry on the Growth and Yield of Tomato(Lycopersicum esculentum Mill.) in Hydroponic Culture (퇴비단 여과액비와 농축액비를 이용한 양액재배가 토마토(Lycopersicum esculentum Mill.)의 생육 및 수량에 미치는 영향)

  • Ryoo, Jong-Won;Seo, Woon-Kab
    • Korean Journal of Organic Agriculture
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    • v.17 no.3
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    • pp.357-370
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    • 2009
  • This experiment was conducted to investigate the effects of compost leachate and concentrated slurry on growth of tomato in hydroponic culture. In process of composting, compost leachate was produced water was through a saturated compost heap. The concentrated slurry was produced by filtration and concentration by membrane process. Filtration of pig slurry was necessary to prevent the hose clogging in hydroponics culture. The treatments of this experiment were consisted of seven different liquid fertilizers; compost leachate(CL), concentrated pig slurry (CS), compost leachate+byproduct(CL+BP), concentrated pig slurry+byproduct(CS+BP), compost leachate 50%+nutrient solution50%(CL+NS), concentrated pig slurry 50%+nutrient solution50%(CS+NS) and nutrient solution(NS) for tomato based on nitrogen content. The chemical nutrient solution was the solution of National Horticulture Research Station for the growth of tomato. The concentration of nutrient solution was adjusted a range of $1.6{\sim}2.0 mS/cm$ in EC. 1. The compost leachate and concentrated pig slurry were low in phosphorus(P), calcium(Ca), magnesium(Mg), but rich in potassium(K). 2. Plant height, SPAD value of tomato was highest in the plot of CS+NS, intermediate in CL, CS+BP, and lowest in 100% concentrated pig slurry. 3. The tomato yield of compost leachate plot was 91% compared with inorganic nutrient solution. The compost leachate solution could be used as a nutrition solution of tomato in organic hydroponics. 4. The growth including plant height, SPAD value, fruit number, fruit weight and yield of tomato in the CL 50%+NS 50% was similar in the control. In conclusion, the mixture solution of 50% pig slurry and 50% nutrient solution could be used as a nutrition solution of tomato hydroponic culture.

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Effect of stem cell culture on thermal stability (줄기세포배양액이 열 안정성에 미치는 영향)

  • Moon, Ji-Sun
    • Journal of the Korean Applied Science and Technology
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    • v.38 no.1
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    • pp.186-195
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    • 2021
  • In this study, when stem cell culture solution is used as a cosmetic ingredient, one of the most prominent problems is that the ingredients generally have low thermal stability. Therefore, in this study, in order to find out how the stem cell culture medium is heated or preserved at high temperature, the effect of various effects of stem cells on the various effects of the stem cells was investigated. Investigated. As a result of the experiment, the wound healing assay confirmed that the cell migration increased after 6 hours, and after 24 hours, it was confirmed that the cell mobility was increased and cell division was promoted, thereby being concentrated. As a result of investigating the amount of transdermal water loss by preparing a cosmetic product containing stem cell culture solution, it was confirmed that the culture solution addition group showed an improvement rate of 31% compared to the non-added group, thereby helping in skin wound recovery. As a result of this, it is considered that this point should be considered when the stem cell culture medium is used as an active ingredient in cosmetics in the future.

Safety study on Genetic Toxicity of Cervi Pantotrichum Cornu Herbal acupuncture Solution(CPCHA) (유전독성시험에 의한 녹용약침의 안전성 연구)

  • Seo, Bu-Il;Byun, Boo-Hyeong
    • The Korea Journal of Herbology
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    • v.20 no.2
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    • pp.1-6
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    • 2005
  • Objectives : The purpose of this study is to investigate genetic toxicity of Cervi pantotrichum Cornu herbal acupuncture solution(CPCHA). Methods : In this study, a series of investigation have been carried out to analyze the effects of Cervi pantotrichum Cornu herball acupuncture solution(CPCHA) on colony forming ability of NIH3T3cells, Hela cells and adrenorectal coloncell for genetic toxicity test. Results and Conclusions : From the above results, it is suggested that Cervi pantotrichum Cornu herball acupuncture solution(CPCHA) was limited 0.5-10ug/ml by test. Cervi pantotrichum Cornu herball acupuncture solution(CPCHA) did not exert the protective role to the genetic toxicity in kinds of cell lines used in this study. From these results, Cervi pantotrichum Cornu herbal aqua-acupuncture solution needs further study to prove it's function in cell culture system.

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A Study on Accumulation of Ni in Salix alba and S. caprea by Hydroponic Culture in Ni Solution (수경재배에 의한 Salix alba와 S. caprea의 Ni축적에 관한 연구)

  • Lee, Chang-Heon
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.11 no.5
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    • pp.1-11
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    • 2008
  • This study was carried out to provide primary data set for cleaning of contamination site. By having hydroponic culture on Salix alba and S. caprea seedling treated with Ni solution, the result of Ni accumulation came out as followings : In a treatment of Ni to the Salix alba and S. caprea, Ni accumulation increased in its root, leaves, and stem, as Ni concentration became higher until $10.0{\mu}mol$. But in a $100.0{\mu}mol$ treatment, the seedlings died after 4 weeks. Ni accumulation in the Salix alba and S. caprea was the highest in its roots, second-highest in leaves, and the lowest in stems. In the case of $10.0{\mu}mol$ treatment of Ni solution, Ni accumulation in roots were above 500.0mg/L, and leaves were above 20.0mg/L. But it was lower than 13.0mg/L in stems. Ni accumulation in the plant increased more when nutrient solution containing Ni was weekly changed than just refilling the same amount of nutrient solution that evaporated Ni accumulation in Salix alba was higher than S. caprea when the nutrient solution had been refilled only.

Effects of Chlorella Culture Solution Using As Midium of Anaerobic Digestate on Early Growth of Italian Ryegrass (Lolium multiflorum L.) (혐기소화처리액을 배지로 이용한 클로렐라 배양액 시용이 이탈리안 라이그라스의 초기생육에 미치는 영향)

  • Seo, Un Kab;Lee, Jin Woong;Ryoo, Jong Won
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.36 no.4
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    • pp.393-401
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    • 2016
  • Anaerobic digestion is a collection of naturally occurring processes that convert organic matter and liquid residue, so-called digestate. The use of digestate biofertilizers is one of the important components of integrated nutrient management, as they are renewable sources of plant nutrients for sustainable agriculture. Seeds of Italian ryegrass (Lolium multiflorum L.) were germinated in different concentration of Chlorella in order to investigate it's the effect of Chlorella on growth parameters, seed germination and early growth. The experiment using plug tray was conducted at the green house placed in the Sangji University. The experiment consisted of nine treatments including different concentrations of Chlorella sp. culture solution and non-treated control. The germination percentage at the treatment with 25% Chlorella sp. culture solution was greater than that of control. The 50% concentration of Chlorella sp. culture solution was found to promote a better seedling growth in terms of shoot length, fresh weight and dry weight compared to the anaerobic digestate. Results showed that the best concentration of Chlorella culture solution was achieved by the 50% concentration of Chlorella culture solution treatment. As a conclusion, the application of Chlorella culture solution was found to be able to promote the germination and shoots growth of Italian ryegrass.

pH Affects the In vitro Formation of cis-9, trans-11 CLA and trans-11 Octadecenoic Acid by Ruminal Bacteria When Incubated with Oilseeds

  • Wang, J.H.;Song, M.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.12
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    • pp.1743-1748
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    • 2003
  • The effect of pH on the fermentation characteristics and the formation of cis-9, trans-11 conjugated linoleic acid (CLA) and trans-11 octadecenoic acid by mixed ruminal bacteria was examined in vitro when incubated with linseed or rapeseed. Concentrate (1%, w/v) with ground linseed (0.6%, w/v) or rapeseed (0.5%, w/v) was added to 600 ml mixed solution of strained rumen fluid with artificial saliva (1:1, v/v), and was incubated anaerobically for 12 h at $39^{\circ}C$. The pH of culture solution was maintained at level close to 4.5, 5.3, 6.1 and 6.9 with 30% $H_2SO_4$ or 30% NaOH solution. pH increment resulted in increases of ammonia and total volatile fatty acid (VFA) concentration in culture solutions containing both oilseeds. Fermentation did not proceeded at pH 4.5. Molar proportion of acetate decreased but that of propionate increased as pH increased when incubated with oilseeds. While the hydrogenating process was very slow at the pH range of 4.5 to 5.3, rapid hydrogenation was found from the culture solutions of pH 6.1 and 6.9 when incubated with linseed or rapeseed. As pH in culture solution of linseed or rapeseed increases proportions of oleic acid (cis-9 $C_{18:1}$) and trans-11 octadecenoic acid increased but those of linoleic acid and linolenic acid decreased. The CLA proportion increased with pH in culture solution containing rapeseed but CLA was mostly not detected from the incubation of linseed.

Microstructure and Mechanical Properties of Solution Treatment and Sr-Modification of Al-12%Si-1.5%Cu Alloy

  • Surin, Prayoon;Wong on, Jessada;Eidhed, Krittee
    • International Journal of Advanced Culture Technology
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    • v.3 no.2
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    • pp.132-137
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    • 2015
  • The purpose of this paper was to investigate the effects of solution treatment time and Sr-modification on the microstructure and property of the Al-Si piston alloy. It was found that as-cast microstructures of unmodified and Sr-modified Al-Si alloys consisted of a coarse acicular plate of eutectic Si, $Cu_3NiAl_6$ and $Mg_2Si$ phases in the ${\alpha}$-Al matrix but different in size and morphology. Both size and inter-particle spacing of Si particles were significantly changed by increasing of the solution treatment time. After a short solution treatment, the coarse acicular plate of the eutectic Si appears to be fragmented. Fully modified microstructure of Sr-modified alloy can reduce the solution treatment time to shorter compared to unmodified alloy. The maximum of a peak hardness value is found in the very short solution treatment of both Al-Si piston alloys. Compared to 10 h solution treatment, the solution treatment of 2-4 h is sufficient to achieve appropriate microstructures and hardness. The short solution treatment is very useful to increase the productivity and to reduce the manufacturing cost of the Al-Si piston alloys.