• Title/Summary/Keyword: Culture collection

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A study on expressive characteristics of graffiti in the Moschino collection by Jeremy Scott (제레미 스캇의 모스키노 컬렉션에 나타난 그라피티 표현 특징에 관한 연구)

  • Bae, Jiye;Kim, Yanghee
    • The Research Journal of the Costume Culture
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    • v.30 no.1
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    • pp.33-49
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    • 2022
  • The purpose of this study is to analyze the expressive elements and techniques of graffiti appearing in the Moschino collection by Jeremy Scott. A theoretical examination of graffiti art and Moschino's creative designer Jeremy Scott was conducted using previous studies and publications. Accordingly, keywords about the expressive elements and techniques of graffiti and Moschino were identified, as follows: expressive elements of 'message (slogan)', 'symbolized letters or forms', 'logo and brand symbol', 'graffiti (scribbles)', 'child-like elements', 'daily element's and expressive techniques of 'using primary colors (color contrast)', 'deformation', 'distortion', 'exaggeration', 'illusion (trompe l'oeil)', 'collage (repetition)', 'simplification (flattening)', and 'borrowing heterogeneous objects'. These keywords were then used to analyze Moschino's collection, comprising seven years of Moschino's collection photographs officially recorded in the fashion magazine Vogue, ranging from the 2014 F/W to 2020 F/W collections. A total of 761 photos were initially collected, from which 561 were selected by the researcher. Expressive characteristics of graffiti in Moschino's collection were analyzed, and identified in the following categories: 'child-like playfulness', 'commercial satire', 'using daily elements', and 'borrowing non-representative techniques'. Accordingly, it was confirmed that expressive characteristics of graffiti were found in the Moschino collection by Jeremy Scott. This study anticipates the possibility of various interpretations from which fashion that communicates closely with contemporary art can be understood.

Molecular and Cultural Characterization of Colletotrichum spp. Causing Bitter Rot of Apples in Korea

  • Lee, Dong-Hyuk;Kim, Dae-Ho;Jeon, Young-Ah;Uhm, Jae-Youl;Hong, Seung-Beom
    • The Plant Pathology Journal
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    • v.23 no.2
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    • pp.37-44
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    • 2007
  • Colletotrichum contains many important pathogens which cause economically significant diseases of crops like pepper, strawberry, tomato and apple. Forty four isolates were collected to characterize the diversity of Colletotrichum causing apple anthracnose in various regions of Korea. They were analyzed by random amplified polymorphic DNA (RAPD), internal transcribed spacer (ITS) of rDNA and partial $\beta$-tubulin gene DNA sequence, and culture characteristics on PDA and PDA-Benomyl. From the results of molecular analyses, 31 strains belonged to Colletotrichum gloeosporioides, ribosomal DNA group (RG) 4 of Moriwaki et al. (2002), 8 strains belonged to C. acutatum, A2 group of Talhinhas et al. (2005) and 5 strains to C. acutatum, A3 group of Talhinhas et al. (2005). Most isolates of C. gloeosporioides RG4 grew faster on PDA than strains of C. acutatum, A2 and A3 groups and most RG4 strains were sensitive to Benomyl. However, a few strains of RG4 grew slower and were resistant to Benomyl. On the basis of molecular characteristics, apple isolates of C. acutatum were clearly differentiated from red pepper isolates of the species, but apple isolates of C. gloeosporioides were not.

Contribution to the Checklist of Soil-inhabiting Fungi in Korea

  • Lee, Seon-Ju;Hong, Seung-Beom;Kim, Chang-Yung
    • Mycobiology
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    • v.31 no.1
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    • pp.9-18
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    • 2003
  • A total of 82 genera and 271 species of soil-inhabiting fungi including saprobic, nematode-trapping, and arbuscular mycorrhizal but plant pathogenic fungi published hitherto in South Korea are listed with the information on geographic location, habitat, vegetation when available, and relevant literatures.

Relationship characteristics of intermedia and fashion show in the Chanel collection (샤넬 컬렉션에 나타난 인터미디어와 패션쇼의 관계적 특성)

  • Lee, Seulah;Lee, Younhee
    • The Research Journal of the Costume Culture
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    • v.24 no.3
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    • pp.367-384
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    • 2016
  • The aim of this paper is to examine relationship characteristics and the relevance there of in the context of the Chanel collection, between the contemporary fashion show viewed from the artistic side and intermedia on interaction. This study analyzed the relationship characteristics between intermedia and fashion shows by focusing on Chanel fashion shows from 2010 S/S to 2016 F/W as actual case. The results are as follow: First, participational accessibility indicates the experience of a designer and audience in active participation changing into passive participation. Second, audience immersion is represented by the perception of being involved by the audience in the place and acting. Third, conceptual symbolism is sending of messages through the designer's concept manifesting in the specific features of the design. Finally, expandability of media explains trans-boundaries in experimental attempts. It concludes that fashion show is a space where the audience can have visual as well as new knowledge and experience through multi-sensory channels. The Chanel collection which is considered a huge art project at the fashion show, showed a relationship between philosophy and the direction through season concepts and brands. The Chanel collection has brought a new experience through the convergence of communications and cultural media, such as music and art according to traditional brand image.

Status of Molecular Biotechnology Research Based on Tissue Culture of Soybean (콩 조직배양 기술에 기반한 생명공학 연구 동향)

  • Seo, Mi-Suk;Cho, Chuloh;Choi, Man-Soo;Chun, JaeBuhm;Jin, Mina;Kim, Dool-Yi
    • Korean Journal of Plant Resources
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    • v.33 no.5
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    • pp.536-549
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    • 2020
  • Soybean (Glycine max (L.) Merrill) is one of the most important crops of the world. With the completion of the soybean genome sequence, the Korean soybean core collection consisted of 430 accessions with genetic and phenotypic diversity was constructed in recent year. The availability of genome sequences and core collection will result in the crop improvement by molecular breeding using the various accessions and genome editing approaches. Efficient tissue culture techniques, such as haploid production, protoplast culture and plant regeneration from various organs are essential for the successful molecular biological approach and crop improvement. However, soybean is still considered to be recalcitrant in tissue culture because of the low frequency of regeneration and limitation of available responsive cultivars. In this study, we discuss the recent studies of tissue culture technology and methodology for efficient tissue culture to genetic improvement and application of molecular biotechnology in soybean.

Screening for Cold-Active Protease-Producing Bacteria from the Culture Collection of Polar Microorganisms and Characterization of Proteolytic Activities (남북극 유래 저온성 박테리아 Culture Collection에서 저온활성 프로테아제 생산균주의 스크리닝과 효소 특성)

  • Kim, Doc-Kyu;Park, Ha-Ju;Lee, Yung-Mi;Hong, Soon-Gyu;Lee, Hong-Kum;Yim, Joung-Han
    • Korean Journal of Microbiology
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    • v.46 no.1
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    • pp.73-79
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    • 2010
  • The Korea Polar Research Institute (KOPRI) has assembled a culture collection of cold-adapted bacterial strains from both the Arctic and Antarctic. To identify excellent protease-producers among the proteolytic bacterial collection (874 strains), 78 strains were selected in advance according to their relative activities and were subsequently re-examined for their extracellular protease activity on $0.1{\times}$ ZoBell plates supplemented with 1% skim milk at various temperatures. This rapid and direct screening method permitted the selection of a small group of 15 cold-adapted bacterial strains, belonging to either the genus Pseudoalteromonas (13 strains) or Flavobacterium (2 strains), that showed proteolytic activities at temperatures ranging between $5-15^{\circ}C$. The cold-active proteases from these strains were classified into four categories (serine protease, aspartic protease, cysteine protease, and metalloprotease) according to the extent of enzymatic inhibition by a class-specific protease inhibitor. Since highly active and/or cold-adapted proteases have the potential for industrial or commercial enzyme development, the protease-producing bacteria selected in this work will be studied as a valuable natural source of new proteases. Our results also highlight the relevance of the Antarctic for the isolation of protease-producing bacteria active at low temperatures.

Kitsch Features Expressed in Contemporary Men's Collection (현대 남성복 컬렉션에 나타난 키치(Kitsch) 특성)

  • Yoo, Hyunseo;Yoon, Jung-A;Lee, Younhee
    • Journal of the Korea Fashion and Costume Design Association
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    • v.17 no.2
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    • pp.11-25
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    • 2015
  • This study analyzed features in contemporary men's collection to provide data for characteristics of kitsch in men's collection. The research method was to see background of the concept of kitsch through the domestic and international publications, the Internet, paper. Five designers' collections that have characteristics of kitsch were selected. The range of collection is from the 2000 S/S to 2011 F/W in Men's collection. The results are as follows: kitsch characteristics could be classified by amusements, inappropriateness, satire. First, exaggerative amusements in men's collection appeared exaggerated form, large size, which is ridiculous at the same time. Infantile amusements represents the image of a pure concentric matching accessories such as toys to the children express. sensual amusements reveals the sexual instincts and desires and people can feel satisfaction through sensual amusements. Second, inappropriateness is characteristic of kitsch to escape from normal life. Clothing does not fit the body and destroyed the design or design of this feature appears. It looks clumsy and strange. Inappropriateness characteristic in men's collection can be divided into inadequate performance and sexual inappropriateness. Third, satire can be divided into resistant satire, imitative satire, and popular satire. Resistance satire with anti-social tendencies through their clothing is achieved by expressing their needs. It was affected by sub-culture like Hippie or Punk. Imitative satire is to imitate other culture, customs and race. It is to resolve complaints through the consumption of things in disparities of wealth and materialism in the society. Popular satire is to express emotions that are free using materials that can recycling like can, bottle or paper.

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Humic Substances Act as Electron Acceptor and Redox Mediator for Microbial Dissimilatory Azoreduction by Shewanella decolorationis S12

  • Hong, Yi-Guo;Guo, Jun;Xu, Zhi-Cheng;Xu, Mei-Ying;Sun, Guo-Ping
    • Journal of Microbiology and Biotechnology
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    • v.17 no.3
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    • pp.428-437
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    • 2007
  • The potential for humic substances to serve as terminal electron acceptors in microbial respiration and the effects of humic substances on microbial azoreduction were investigated. The dissimilatory azoreducing microorganism Shewanella decolorationis S12 was able to conserve energy to support growth from electron transport to humics coupled to the oxidation of various organic substances or $H_2$. Batch experiments suggested that when the concentration of anthraquinone-2-sulfonate (AQS), a humics analog, was lower than 3 mmol/l, azoreduction of strain S12 was accelerated under anaerobic condition. However, there was obvious inhibition to azoreduction when the concentration of the AQS was higher than 5 mmol/l. Another humics analog, anthraquinone-2-sulfonate (AQDS), could still prominently accelerate azoreduction, even when the concentration was up to 12 mmol/l, but the rate of acceleration gradually decreased with the increasing concentration of the AQDS. Toxic experiments revealed that AQS can inhibit growth of strain S12 if the concentration past a critical one, but AQDS had no effect on the metabolism and growth of strain S12 although the concentration was up to 20 mmol/l. These results demonstrated that a low concentration of humic substances not only could serve as the terminal electron acceptors for conserving energy for growth, but also act as redox mediator shuttling electrons for the anaerobic azoreduction by S. decolorationis S12. However, a high concentration of humic substances could inhibit the bacterial azoreduction, resulting on the one hand from the toxic effect on cell metabolism and growth, and on the other hand from competion with azo dyes for electrons as electron acceptor.

Alternative Splicing of Breast Cancer Associated Gene BRCA1 from Breast Cancer Cell Line

  • Lixia, Miao;Zhijian, Cao;Chao, Shen;Chaojiang, Gu;Congyi, Zheng
    • BMB Reports
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    • v.40 no.1
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    • pp.15-21
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    • 2007
  • Breast cancer is the most common malignancy among women, and mutations in the BRCA1 gene produce increased susceptibility to these malignancies in certain families. In this study, the forward 1-13 exons of breast cancer associated gene BRCA1 were cloned from breast cancer cell line ZR-75-30 by RT-PCR method. Sequence analysis showed that nine BRCA1 splice forms were isolated and characterized, compared with wild-type BRCA1 gene, five splice forms of which were novel. These splice isoforms were produced from the molecular mechanism of 5' and 3' alternative splicing. All these splice forms deleting exon 11b and the locations of alternative splicing were focused on two parts:one was exons 2 and 3, and the other was exons 9 and 10. These splice forms accorded with GT-AG rule. Most these BRCA1 splice variants still kept the original reading frame. Western blot analysis indicated that some BRCA1 splice variants were expressed in ZR-75-30 cell line at the protein level. In addition, we confirmed the presence of these new transcripts of BRCA1 gene in MDA-MB-435S, K562, Hela, HLA, HIC, H9, Jurkat and human fetus samples by RT-PCR analysis. These results suggested that breast cancer associated gene BRCA1 may have unexpectedly a large number of splice variants. We hypothesized that alternative splicing of BRCA1 possibly plays a major role in the tumorigenesis of breast and/or ovarian cancer. Thus, the identification of cancer-specific splice forms will provide a novel source for the discovery of diagnostic or prognostic biomarkers and tumor antigens suitable as targets for therapeutic intervention.