• 제목/요약/키워드: Culture characterization

검색결과 928건 처리시간 0.022초

영지버섯 백색 변이주의 형태적 특성 및 최적 배양조건 (Morphological Characterization and Culture Conditions of A White Mutant of Ganoderma Iucidum)

  • 조수묵;서건식;유승헌;유익동;신관철
    • 한국미생물·생명공학회지
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    • 제21권6호
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    • pp.520-526
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    • 1993
  • A morphologically different form of Ganoderma lucidum was isolated from a cultivator's farm, and its optimum growth conditions were determined. A major difference in their morphology was color of fruit bodies. Fruit bodies of the mutant were white wherase those of normal Ganoderma lucidum were red. Spores of the mutant were global and mycelia were thin. Mycelial growth of this white mutant was favorable on potato sucrose agar medium, and optimum pH of the medium was 5.5.

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문화위성계정 체계 (System of Culture Satellite Account)

  • 김지영
    • Communications for Statistical Applications and Methods
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    • 제17권6호
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    • pp.879-889
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    • 2010
  • 본 논문은 국민계정의 문화부문 위성계정인 문화위성계정의 체계와 우리나라가 문화위성계정을 작성하기 위해 필요한 사항 등을 내용으로 한다. 문화위성계정은 각기 개별적 기능을 갖는 5개 부문으로 구성하도록 권고되고 있다. 동 권고안에 의하면 제 1부문부터 제 4부문까지는 각각 해당부문의 공급과 수요측면의 통계를 수록하도록 되어 있고, 제 5부문은 참고사항 모듈(documentation module)로 편제하도록 권고되고 있다. 문화위성계정의 제 1부문은 문화활동 개요 모듈(macro-information module), 제 2부문은 문화생산량 모듈(quantity/volume output module), 제 3부문은 특성 모듈(characterization module), 제 4부문은 목표분석 모듈(targeted analysis/analytical module)로 구성된다.

In Vitro Mycorrhization and Morphological Characterization of Xanthoconium affine with Pinus densiflora

  • Wang, Eun-Jin;Jang, Yeongseon;Ka, Kang-Hyeon
    • 한국균학회지
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    • 제45권4호
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    • pp.319-327
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    • 2017
  • In this study, we investigated the culture conditions of four ectomycorrhizal fungi, namely, Amanita spissacea NIFoS 2719, Pisolithus arhizus NIFoS 2784, Suillus spraguei NIFoS 2848, and Xanthoconium affine NIFoS 2716, in solid and liquid culture media. In addition, we attempted to induce in vitro mycorrhization of the fungi with Pinus densiflora. Prior to liquid culture, we determined the optimal culture conditions for each species in solid media. The results revealed that all species examined are capable of growth in potato dextrose agar (PDA), malt extract agar (MEA), and modified Melin-Norkran's medium (MMN), although their preferred growth media were different. Liquid culture experiments showed that inorganic nitrogen did not enhance the mycelial growth of all four species. Therefore, we used MMN-based liquid inocula to promote the growth of ectomycorrhizal fungi in our symbiosis culture system. Mycorrhization was observed in Xanthoconium affine NIFoS 2716. Morphological analysis revealed that fungi-inoculated roots of P. densiflora form simple and dichotomous lateral roots with dense mycelia. In addition, inoculation with X. affine NIFoS 2716 promoted root and shoot developments.

Cultural characterization of probiotic Lactobacillus sakei BK19

  • Yang , Byung Gyoo;Song , Choon Bok;Yeo , In Kyu;Lee , Kyoung Jun;Park , Geun Tae;Lee, Sang Hyeon;Son, Hong Joo;Heo, Moon Soo
    • 한국어병학회지
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    • 제16권2호
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    • pp.119-123
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    • 2003
  • We have selected an valuable pmbiotic strain; Lactobacillus sakei BK19 which has wide antagonic spectrum against fish pathogens . Present study investigated cultural characterization of L. sakei BK19 including pH tolerance , susceptibility of antibacterial agents and growth pattern with different environment such as nutritions, temperature and salinity. L. sakei BK19 showed Significantly higher resistance at low pH(around pH 4) environment and relative high antibiotic tolerance . In the study of optimal culture condition, maltose and saccharose provided the optimal nutritional culture condition while lactose and mannitol were unable to supply its carbon source for the fermentation of L. sakei BK19. Moreover. L. sakei BK19 showed good growth at the temperature range of 15 to $45^{o}C$ und the NaCl concentration of 0 to 7%. Hence, this particular probiotic strain may be benificial both in seawater and fresh weter conditions.

In vitro culture of chicken embryonic stem cell-like cells

  • Bo Ram Lee;Hyeon Yang
    • 한국동물생명공학회지
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    • 제38권1호
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    • pp.26-31
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    • 2023
  • Chicken embryonic stem (ES) cells have great potential and provide a powerful tool to investigate embryonic development and to manipulate genetic modification in a genome. However, very limited studies are available on the functional characterization and robust expansion of chicken ES cells compared to other species. Here, we have developed a method to generate chicken embryonic stem cell-like cells under pluripotent culture conditions. The chicken embryonic stem cell-like cells were cultivated long-term over several passages of culture without loss of pluripotency in vitro and had the specific expression of key stem cell markers. Furthermore, they showed severe changes in morphology and a significant reduction in pluripotent genes after siRNA-mediated NANOG knockdown. Collectively, these results demonstrate the efficient generation of chicken embryonic stem cell-like cells from EGK stage X blastoderm-derived singularized cells and will facilitate their potential use for various purposes, such as biobanking genetic materials and understanding stemness in the fields of animal biotechnology.

Molecular and Cultural Characterization of Colletotrichum spp. Causing Bitter Rot of Apples in Korea

  • Lee, Dong-Hyuk;Kim, Dae-Ho;Jeon, Young-Ah;Uhm, Jae-Youl;Hong, Seung-Beom
    • The Plant Pathology Journal
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    • 제23권2호
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    • pp.37-44
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    • 2007
  • Colletotrichum contains many important pathogens which cause economically significant diseases of crops like pepper, strawberry, tomato and apple. Forty four isolates were collected to characterize the diversity of Colletotrichum causing apple anthracnose in various regions of Korea. They were analyzed by random amplified polymorphic DNA (RAPD), internal transcribed spacer (ITS) of rDNA and partial $\beta$-tubulin gene DNA sequence, and culture characteristics on PDA and PDA-Benomyl. From the results of molecular analyses, 31 strains belonged to Colletotrichum gloeosporioides, ribosomal DNA group (RG) 4 of Moriwaki et al. (2002), 8 strains belonged to C. acutatum, A2 group of Talhinhas et al. (2005) and 5 strains to C. acutatum, A3 group of Talhinhas et al. (2005). Most isolates of C. gloeosporioides RG4 grew faster on PDA than strains of C. acutatum, A2 and A3 groups and most RG4 strains were sensitive to Benomyl. However, a few strains of RG4 grew slower and were resistant to Benomyl. On the basis of molecular characteristics, apple isolates of C. acutatum were clearly differentiated from red pepper isolates of the species, but apple isolates of C. gloeosporioides were not.