• 제목/요약/키워드: Culture Fusion

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The Presence in Embryo Extract of a Myotrophic Protein That Affects Proliferation and Fusion of Chick Embryonic Myoblasts in Culture (배양 계배 근원세포의 분화에 미치는 계배 추출물내 Myotrophic Protein의 영향)

  • 유병제;이창호;곽규봉;정진하;하두봉
    • The Korean Journal of Zoology
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    • v.31 no.3
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    • pp.207-217
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    • 1988
  • A myotrophic protein that seemed to he eseentiai for the hision of chick embryonic myoblasts in culture was isolated from chick embryo extrad and was found to be identical or at least similar to the iron-transporting protein, transferrin. Embryo extract seemed to contain, in addition to this myotrophic protein, a heat stable protein that inhibits the fusion of myoblasts. Iron seemed to he necessary for myoblasts to fuse and it was supposed that the role of the myotrophic protein m myoblast fusion is to supply iron to the cell. The numher of the myotrophic protein receptors on myoblast surface membrane decreased immediately after the start of myoblast fusion, supposedly due to the decreased need of iron after the fusion once commenced. It was estimated that endocytosis of myotrophic protein took about 10 minutes and one recycling about 2 hours. The accumulation of iron in myoblasts continued linearly with cultre time and endocytosis of the myotrophic protein occured at a constant rate.

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Improvement of Plants by Biotechnology (세포공학을 이용한 식물개량)

  • 윤의수
    • Korean Journal of Plant Resources
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    • v.3 no.1
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    • pp.1-30
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    • 1990
  • The traditional plant imprownent methods consisted of pure line selection, cross breeding, heterosis breeding, polyploid breeding, mutati-onbreeding, ect.Biotechmoiogy is divided into gene spliclng , monocle-nal antibodies , protein engineering , agricultural research, and microbiological engineering. Of these , high plants deal with agricultural research, and the importent part of which is tissue culture and celLculture , Tissue .culture and cell culture are again divided into embryoculture, test tube fertilization, anther and pollen culture, somatichybridization , transformation, recombination, recombinant DNA moleculehybrid plasmid, ect For these haploid production, protoplast culture,protoplast fusion, selection and propagation, ect. , the technical sett-lement is needed.

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A Study on the Modern Fashion Reflected Art of the Tang Dynasty (당대(唐代) 예술(藝術)이 반영(反映)된 현대(現代)패션에 관(關)한 연구(硏究) - 1998년(年)부터 2000년(年) Collection을 중심(中心)으로 -)

  • Kan, Ho-Sup;Suh, Yun-Hee
    • Journal of Fashion Business
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    • v.4 no.4
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    • pp.55-70
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    • 2000
  • The purpose of this thesis is to suggest the new orientalism fashion through the comprehensive approach to analyze the art of the Tang dynasty, China. Tang dynasty has high culture, economy, polity and art. Also the character of Tang's culture is very international. Therefore that point is coincide with fusion culture in the early 2000's. As a method of accomplishing this research, the documents and fashion magazines related to the art and fashion were examined. Through analyzing the art in the Tang's related to fusion culture, reach a conclusion that the modern fashion has four characters. 1. The cause of high culture and economy, the trend of fashion was decorative 2. The cause of realism, the trend of fashion were modern and simple. 3. The cause of rhythmical line, the trend of fashion had natural drape. 4. The cause of globalism, the trend of fashion mixed east with west This research keep going for showing new orientalism and coinciding with 21th century's trend.

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Production of Cloned Embryos by Nuclei Transfer and Electronic Cell Fusion from Bovine Fetal Fibroblasts (Bovine Fetal Fibroblasts를 이용한 핵이식 및 세포융합에 관한 연구)

  • 이병천;박종임;조종기;김기연;신수정;용환율;황우석
    • Journal of Embryo Transfer
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    • v.14 no.2
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    • pp.107-111
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    • 1999
  • The present study was performed to evaluate the best electric fusion condition in nuclear transfer, Korean Native Cattle fibroblasts were used as nucleic donors. Oocytes from slaughterhouse were matured in vitro for 22 h and enucleated. Each individual cells were transferred into enucleated ocytes and reconstructed embryo were placed into the fusion chamber. In experiment 1, pulse were performed by altering pulse duration at 1. 75kv/cm, 1 time. When pulse duration is 30$mutextrm{s}$, fusion and development rates is higher than other conditions. In experiment 2, the effect of different pulse number were studied at the pulse duration 30$mutextrm{s}$ and the same pulse intensity. When pulse number was one, fusion rates were higher than other conditions. The fused embryos were moved to culture medium and assessed their development to blastocyst. These results showed that best fusion condition was 30$mutextrm{s}$ and one time. And the fibroblasts derived from Han Woo can be reprogrammed by nuclear transplantation and develop subsequently in vitro.

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A Study on Costume Culture Interchange Resulting from Political Factors (정치적 요인에 의한 복식문화교류에 관한 연구)

  • Yu Ju-Ri;Kim Jeong-Mee
    • Journal of the Korean Society of Clothing and Textiles
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    • v.30 no.3 s.151
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    • pp.458-469
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    • 2006
  • The purpose of this study was to prove that interchange is a primary factor in costume changes through case analysis in costume culture interchange, and further, to assist in gaining understanding of costume changes of the present and the future, and therefore, in this study, cases analysis was conducted focusing on interchange resulting from political factors. In order to present theories that are able to analyse costume culture interchange, culture, cultural exchange, and costume cultures were examined, and based on the results, a costume culture interchange process model and its three steps, propagation, selection, and reinterpretation, were presented. The results of case study on costume culture interchange resulting from political factors based on the costume culture interchange process model presented are as follows. Interchanges that politically uses the possibility of expressing costume as symbols are in most cases aggressive and semi aggressive. In order to exhibit superiority of themselves, the aggressors forced their national costumes onto the receiving culture. Rather than a simple introduction of their costumes, it was an attempt to introduce they values and their ways of life. The conclusion that can be reached through such result is that interchange is an important driving force for changes in costume culture. All interchanges were not greatly affected by methods of propagation and acceptance, and resulted in fusion. Fusion is a process in which existing costume and foreign costume come together constructively to for a new costume culture therefore change in costume style is inevitable.

Effects of Oviductal Fluid, Culture Media and Zona Pellucida Removal on the Development of Porcine Embryos by Nuclear Transfer

  • Zhang, Y.H.;Song, E.S.;Kim, E.S.;Cong, P.Q.;Lee, S.H.;Lee, J.W.;Yi, Y.J.;Park, Chang-Sik
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.7
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    • pp.962-968
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    • 2009
  • The aim of this study was to compare the effects of oviductal fluid, porcine zygote medium (PZM)-3, PZM-4 and PZM-5, and modified PZM-5 culture media, and determine the effects of zona pellucida (ZP) removal on the development of nuclear transfer (NT) embryos. There were no significant differences in the rates of fusion and cleavage among the five different oviductal fluid concentrations. However, the rates of blastocyst formation and the cell numbers per blastocyst were high in the embryos at the 14 and 28 $\mu{g}$/ml concentrations of oviductal fluid compared to the 0, 56 and 100 $\mu{g}$/ml concentrations. The rates of cleavage and blastocyst formation, and the cell numbers per blastocyst were higher in the PZM-3, PZM-5 and modified PZM-5 media than in the PZM-4 medium. However, there were no significant differences in the fusion rates of oocytes among the four culture media. The cell numbers per blastocyst in the embryos without ZP were significantly greater than those with ZP. However, there were no significant differences in the rates of fusion, cleavage and blastocyst formation between the embryos with and without ZP. In conclusion, we improved blastocyst development and the quality of NT embryos by replacing PVA with 3 mg/ml of BSA in PZM-5 medium and supplementing the PZM-5 medium with 14 $\mu{g}$/ml oviductal fluid. The NT embryos produced by the zona-free NT method had a high rate of blastocyst formation in the modified PZM-5 medium.

A Study on The Plastic Characteristic of Furniture Design Using Cyberspace (사이버공간을 이용한 가구디자인의 조형적 특성에 관한 연구)

  • Kim, Gun-Soo
    • Journal of the Korea Furniture Society
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    • v.21 no.4
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    • pp.326-337
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    • 2010
  • The invention and development of digital computer networks since the post-modern culture and creating new ideas, and in nearly all areas since the industrial revolution has had the greatest impact. A new paradigm for the 21st century, the Internet and ubiquitous, and mobile devices are being created by the new culture is being created. Rapidly being replaced by digital and analog, and 'Digilog' fusion concept has appeared. In addition, the development of computer cyberspace culture, art, design and structure are changing. In the field of furniture design, changing design processes and innovative design philosophy that change is now occurring. These symptoms experienced at the same time, almost every country on Earth is a phenomenon. Since the 20th century, computer network design based on the relationship between digital and cyber culture espoused by philosophers studied society and organic modernism and organic design of digital-based differences are confirmed through the case. Using digital technology, modeling case study of a furniture designer in cyber space and the creation of human capabilities and the fusion of computing power will be studied in modern furniture design.

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A Study on the Remediation of Game Culture in Video Media (영상미디어의 게임문화 재매개 양상 연구)

  • Lee, Dong-Eun
    • Journal of Korea Game Society
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    • v.21 no.6
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    • pp.97-110
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    • 2021
  • The purpose of this study is to analyze how video media such as movies and dramas, which aim for one-way and viewing-oriented content, remediates the attributes of games, and to study the influence and convergence of games and video media. There were two main types of game culture convergence patterns of video media. The first is the passive fusion of the content aspect of the game, and the second is the active fusion of the system aspect of the game. Such research is significant as a basic study that reveals that games affect various video media and proves that game media can function as a representative media leading popular culture.

Isolation, Culture, and Fusion of Nicotiana Protoplasts (원형질체 분리, 배양 및 Nicotiana 종간 세포융합에 관한 연구)

  • 윤경은;김준철;최상수;손세호
    • Journal of the Korean Society of Tobacco Science
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    • v.1 no.2
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    • pp.138-149
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    • 1979
  • For the preliminary study on tobacco cell fusion as one of new breeding techniques, the conditions that would be most effective in isolation, fusion, and culture of tobacco protoplasts were examined ; 1. The enzyme solution of 0.5% macerozyme and 2% cellulase( or meicellase) was the most economic and efficient in isolating protoplasts from tobacco leaves. 2. The proper incubation period of tobacco leaves in cell wall digesting solution was 4 hours. 3. As an osmotic stabilizer, sorbitol or mannitol solutions were employed. The concentration of 0.5~0.7 M of either hexitol gave satisfying results as the osmotic stabilizer. 4. The calcium concentration appeared to be an important factor in protoplast fusion. The adhesion of protoplasts was enhanced by enrichment of calcium ion in PEG solution. The highest frequency of protoplast fusion was obtained when tobacco protoplasts were incubated in PEG solution. containing 9mM CaCl2. 5. Cell divisions of the isolated protoplasts were continued and have generated colonies when they were grown on B-5 medium at 28$^{\circ}C$.

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