• Journal of Microbiology and Biotechnology
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• v.18 no.5
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• pp.913-917
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• 2008

The cyclic undecapeptide, cyclosporin A (CyA), is one of the most commonly prescribed immunosuppressive drugs. It is generated nonribosomally from a multifunctional cyclosporin synthetase enzyme complex by the filamentous fungus Tolypocladium niveum. In order to maximize the production of CyA by wild-type T. niveum (ATCC 34921), each of three culture stages (sporulation culture, growth culture, and production culture) were sequentially optimized. Among the three potential sporulation media, the SSMA medium generated the highest numbers of T. niveum spores. The SSM and SM media were then selected as the optimal growth and production culture media, respectively. The addition of valine and fructose to the SM production medium was also determined to be crucial for CyA biosynthesis. In this optimized three-stage culture system, 3% of the spore inoculum generated the highest level of CyA productivity in a 15-day T. niveum production culture, thereby implying that the determination of an appropriate size of T. niveum spore inoculum plays a critical role in the maximization of CyA production.

• Journal of Families and Better Life
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• v.27 no.1
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• pp.107-125
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• 2009

This paper analyzes the life and culture contents of the primary Korean textbook for female marriage immigrants and investigates ways to improve the textbook organization in future. The study results are as follows: 1. The textbook must reflect the demands of female marriage immigrants as learners that need to adapt to Korean society. 2. The textbook needs to adopt a circulating disposition in organizing such life and culture contents that female marriage immigrants can obtain and utilize while living in diverse life environments 3. The textbook should also provide multi-cultural elements in order to help female marriage immigrants with the internalization of the complex identity between their home countries and Korea.

• Journal of Chest Surgery
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• v.23 no.4
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• pp.622-639
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• 1990

The use of aortic valve homograft has been developed since 1962 when Ross and Barratt - Boyes independently replaced a diseased aortic valve with an orthotopically inserted homograft valve. And also surgical treatment of complex congenital cardiac malformations utilizing homograft extracardiac conduit has been tried with better result than any other prosthetic material. The present study was undertaken to clarify the safety tissue viability, sterility, after following our protocol of procurement of heart, dissection of aortic and pulmonic homograft, sterilization, cryopreservation, thawing and dilution, and transplantation on experimental animal, sheep. Tissue viability of valve and great artery was assessed by tissue culture. Sterility was evaluated by bacterial and fungal culture. The method used was proven no deleterious effect on the integrity of the valve. Tissue culture of valve tissue before, and after cryopreservation process resulted that active fibroblast growth was observed from homograft sterilized with antibiotics. And culture of the transplanted homograft from sacrificed animal showed active fibroblast growth. Pathologic examination of implanted valve tissue from sacrificed sheep showed mild calcification and minor change, but there were moderate and severe calcification of wall of great arteries.

• Microbiology and Biotechnology Letters
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• v.21 no.2
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• pp.144-149
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• 1993

We have identified a T cell-activating material in the culture supernatant of Streptomyces species. The factor in microbial culture supernatant (MCS) induced thymocyte proliferation in a does dependent fashion and it could be detected by immunoblot analysis using anti-interleukin-1(IL-1) antibody. The factor in MCS was slightly larger(about 21 kd) in its molecular weight than IL-1 on SDS-PAGE. When 125I-MCS was covalently coupled with homo-bifunctional cross-linking agent, disuccinimidyl-propionate to IL-1 receptor(IL-1R) on mouse thymoma cell(EL-4) and immunoprecipitated with anti-IL-1R antibody the molecular weight of this complex of 110 kd was observed.

• International Journal of Computer Science & Network Security
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• v.21 no.12spc
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• pp.473-476
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• 2021

The article used a complex system of research methods: observation of the course of the educational process, analysis of literature on pedagogy, teaching methods, modern pedagogical technologies; analysis of the experience of using multimedia in the study of disciplines. The theoretical significance of the article: the definition of multimedia programs is given, the advantages and disadvantages of using multimedia programs are revealed, the basic principles of teaching using multimedia programs are revealed.

• The Journal of the Korea Contents Association
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• v.19 no.12
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• pp.151-163
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• 2019

The purpose of this study was to investigate the effects of safety culture on the safety consciousness and safety behaviors of manufacturing workers, and to suggest ways for manufacturing workers to understand the safety culture and improve safety consciousness and safety behavior. To achieve this research objective, out of 176 industrial complexes in Seoul and Gyeonggi Province registered with the Korea Industrial Complex Corporation, workers at 50 industrial complexes, including the Korea Export-Industrial Complex and Seoul's Onsuji, were subject to research. The implementation of this survey distributed 300 questionnaires through the mid-level managers of manufacturers and environment safety and health managers over a period of one month from August 1st to 31st, 2019, finally statisticalizing the data in 282 parts excluding 18 questionnaires deemed to have been unresponsive. First, the safety culture (safety climate, safety procedures) has a positive effect on the safety consciousness (recognition of importance, interest and participation inducement) of the manufacturing workers. Second, the safety culture (safety climate, safety procedures) has a positive effect on the safety behavior (safety planning, safety check) of manufacturing workers. Third, safety consciousness (recognition of importance, interest and inducement of participation) has a positive influence on the safety behavior (safety planning, safety check) of manufacturing workers. Fourth, the safety consciousness (recognition of importance, interest and inducement of participation) appears to have a partial mediating effect in relation to safety culture (safety climate, safety procedure) and safety behavior (safety planning, safety check) of manufacturing workers. The implication of this study is that although the industrial accidents have occurred in the manufacturing industry in recent years, the studies on the workers in the manufacturing industry are insufficient. However, this study is meaningful that it has suggested ways for manufacturing workers to understand the safety culture and improve the safety consciousness and safety behavior by analyzing the effects of safety culture on safety consciousness and safety behavior of manufacturing workers.

• Journal of the Korean Society for Library and Information Science
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• v.53 no.4
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• pp.23-50
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• 2019

Libraries have recently sought to diversify their roles to meet the changing needs of local residents, while faithfully playing the library's original roles. The creation of a complex cultural space and the provision of services based on it are one of the ways to expand its role. In this study, a surveyed and analyzed the awareness of the complex cultural space of the library and the preference and awareness of the program operated in the complex cultural space by conducting a survey on the users of the library currently operating the complex cultural space. As a result, first, it was found that they preferred information space, education space, and rest space in space preference. On the other hand, community space and experience space showed low preference. Second, they prefer educational programs, exhibition programs, and performance programs that utilize complex cultural spaces, but experience programs and community programs have low preference. Therefore, it is necessary to operate the program by providing exhibitions and performance spaces while providing information and education spaces to users. The librarian should also promote the fact that library can be only the space of information and education, but also the space where culture, healing, experience and communication take place. In addition, libraries should strive to improve user awareness of library space.

• The Journal of the Convergence on Culture Technology
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• v.9 no.1
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• pp.717-724
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• 2023

we are investigated the anti-inflammatory effects of Safflower and Mother wort Ethanol Complex Extracts(SEC) on lipopolysaccharide (LPS)-activated RAW 264.7 cells. The results demonstrated that pretreatment of SEC(500㎍/mL) significantly reduced NO production by suppressing iNOS protein expression in LPS-stimulated cells. Anti-inflammatory effects by Safflower and Mother wort Ethanol Complex Extracts were observed in the following. Safflower and Mother wort Ethanol Complex Extracts inhibited the translocation of NF-κB from the cytosol to the nucleus via the suppression of IκB-α phosphorylation and also inhibited LPS-stimulated NF-κB transcriptional activity. These findings suggest that Safflower and Mother wort Ethanol Complex Extracts exert anti-inflammatory actions and help to elucidate the mechanisms underlying the potential therapeutic values of Safflower and Mother wort Ethanol Complex Extracts. Therefore, Safflower and Mother wort Ethanol Complex Extracts could be regarded as a potential source of natural anti-inflammatory agents.

• Journal of Microbiology and Biotechnology
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• v.32 no.11
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• pp.1416-1426
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• 2022

The need to discover new types of antimicrobial agents has grown since the emergence of antibiotic-resistant pathogens that threaten human health. The world's oceans, comprising complex niches of biodiversity, are a promising environment from which to extract new antibiotics-like compounds. In this study, we newly isolated Pseudomonas sp. NIBR-H-19 from the gut of the sea roach Ligia exotica and present both phenotypes and genomic information consisting of 6,184,379 bp in a single chromosome possessing a total of 5,644 protein-coding genes. Genomic analysis of the isolated species revealed that numerous genes involved in antimicrobial secondary metabolites are predicted throughout the whole genome. Moreover, our analysis showed that among twenty-five pathogenic bacteria, the growth of three pathogens, including Staphylococcus aureus, Streptococcus hominis and Rhodococcus equi, was significantly inhibited by the culture of Pseudomonas sp. NIBR-H-19. The characterization of marine microorganisms with biochemical assays and genomics tools will help uncover the biosynthesis and action mechanism of antimicrobial metabolites for development as antagonistic probiotics against fish pathogens in an aquatic culture system.