• 제목/요약/키워드: Cultivation region

검색결과 451건 처리시간 0.029초

Development of Protein Biomarkers for the Authentication of Organic Rice

  • Lee, Ju-Young;Lim, Jinkyu
    • Journal of Applied Biological Chemistry
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    • 제58권4호
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    • pp.355-361
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    • 2015
  • The rice protein profiles of Oryza sativa L (Koshihikari) grown under organic and conventional cultivation regimes were compared on 2-D gels to develop diagnostic marker proteins for organic rice. The selected proteins, differentially expressed between organic and conventional rice, were compared with the differentially expressed proteins of another organic and conventional rice pairing, produced at a different location. In the first comparison among conventional, no-chemical, and organic rice grown in the same region, Korea, 13 proteins exhibiting differential expression in organic and conventionally grown plants were selected. Eight of the 13 proteins were down-regulated, and the 5 remaining proteins were up-regulated from conventional to organic rice. The second comparison pairing from Kyungju, revealed 12 differentially expressed proteins, with 8 down-regulated and 4 up-regulated proteins. Ten of the differentially expressed proteins that overlapped between the two comparison sets could not be clustered into any functional group using a functional annotation clustering tool. Further comparisons using another set of conventional and organic rice, belonging to a different variety of Oryza sativa L and produced in Sanchung, revealed 8 differentially expressed proteins, 5 of which were down-regulated and 3 of which were upregulated in the organic rice. Overall, 3 differentially expressed proteins were commonly found in all three organic rice crops. These 3 proteins, along with other overlapping differentially expressed proteins, can provide a good starting point for the development of signature proteins that can be used for the authentication of organic rice with a follow-up studies with more comparison sets.

아무르 주의 사료작물 재배에 대한 일고 - FTA 체결에 대한 대비책의 일환 - (A Study on the Cultivation of Forage Crops in Amur Oblast - An Alternative Measure of Preparations for Conclusion of the FTA -)

  • 김종헌
    • 한국초지조사료학회지
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    • 제27권1호
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    • pp.67-76
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    • 2007
  • The conclusion of free Trade Agreement between South Korea and USA can become a serious threat to the Korean agriculture. On the strategic scheme the foodstuffs is the basis for protection of the state sovereignty. The Amur oblast of Russian Federation is region, which can supply South Korea cheap and good qualitative feeds. It means South Korea should create agricultural cooperation with the Amur oblast of Russian Federation with the purpose to begin organic agriculture in this area and it will help South Korea to counteract a quantitative attack on Korea from the side of american agricultural production. In the Amur oblast there are following fodder crops; Alfalfa, Smooth brome grass (gromus adonis L.), Siberian couch grass, Sudan pass, Colza rape (Brassica napus) and Amaranth (Avfranthus). In the same area also exist oatmeal, wheat, corn and barley, which is possible to use for feeds. All these above written cultures are cultivated organically, therefore it is possible to use them as organic forages in South Korea. It is very important to know system of crop rotation in the Amur oblast. There is a scheme of crop rotation, which is connected with increase and preservation of fertility of soil, maintenance of growth of productivity of agricultural cultures and reception of high-quality production. It is necessary to begin organic agriculture in a southern part of Amur oblast, where, in 2001 year, 97% of all sowing areas was concentrated. The acidity degree of soil in this part is not high ($ph5.1{\sim}5.5$), therefore does not require liming.

Expression of the Functional Recombinant Interleukin-16 in E. coli and Mammalian Cell Lines

  • Kim, Seon-Young;Lee, Chang-Hun;Kim, Kyung-Joo;Kim, Yeon-Soo
    • Journal of Microbiology and Biotechnology
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    • 제11권2호
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    • pp.234-241
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    • 2001
  • The C-terminal 393 bp region of the human interleukin-16 (IL-16) gene was cloned and expressed in E. coli along with mammalian cell lines. Recombinant IL-16 expressed from E. coli was 22 kDa on SDS-PAGE and showed 260% of chemoattractant activity at a concentration of $0.1\;{\mu}g/ml$. HeLa, COS, and Neuro-2a cells were transduced by recombinant retrovirus vector pLNC/IL-16/IRES/TK and the intracellular and secreted amounts of IL-16 produced by HeLa/IL-16/TK, COS/IL-16/TK, and Neuro-2a/IL-16/TK cells were determined by enzyme-linked immunosorbent assay (ELISA). HeLa/IL-16/TK $(1{\times}10^5)$ and COS/IL-16/TK $(1{\times}10^5)$ cells secreted 36.1 and 13.3 ng of IL-16 for 48 h, respectively. Forty-nine ng and 86.4 ng of IL-16 remained in the cell lysates of HeLa/IL-16/TK and COS/IL-16/TK. Intracellular and secreted amounts of IL-16 from Neuro-2a/IL-16/TK $(5{\times}10^5)$ cells during 24 h cultivation were 50 ng and 3.3 ng, respectively. Also, HeLa and COS cells wee stably transfected with mammalian expression vector pCRIII/IL-16. Both culture media and cell lysates prepared from HeLa/IL-16 cells and COS/IL-16 cells showed chemoattractant activity ranging from 190% to 460% as compared to the control experiment. Expression of the herpes simplex virus thymidine kinase (HSV0tk) gene in pLNC/IL-16/ IRES/TK bicistronic retroviral expression vector was verified by performing a genciclovir (GCV) sensitivity assay. Finally, IL-16 repressed Tat-transactivated human immunodeficiency virus type 1 long terminal repeat (HIV-1 LTR) promoter activity.

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Pichia pastoris에서 Aspergillus ficuum 유래 Acetyl Xylan Esterase 유전자의 과발현 (High-Level Expression of Aspergillus ficuum Acetyl Xylan Esterase Gene in Pichia pastoris,)

  • 임재명;김성구;박승문;남수완
    • 한국미생물·생명공학회지
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    • 제30권4호
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    • pp.305-311
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    • 2002
  • Aspergillus ficuum 유래 acetyl xylan esterase(AXEase) 유전자(AXE)를 Pichia pastoris에서 과발현ㆍ분비 생산하기 위해 AOXI promoter와 mating factor $\alpha$-1 분비신호서열 하류에 AXE를 연결한 염색체 삽입 발현계(pPICZ$\alpha$C-AXE, 4.6 kb)를 구축하였다. 이것을 SacI으로 절단한 뒤 P. pastoris의 염색체 DNA 5'AOX1 부위에 삽입시켰다. 형질전환된 P. pastoris 균주를 메탄을 배지에서 플라스크 회분배양한 결과, 배양 36시간 때의 건조균체농도는 6 g-DCW/1, AXEase 총 발현량은 77 unit/ml이었다. 최적화된 methanol과 histidine 공급방법을 채용한 유가배양시 균체농도는 97 g-DCW/1, AXEase 총발현량은 930 unit/m1로 크게 증가하였다. 효소활성의 90% 이상은 배양 상등액에 존재하였으며, 상등액 단백질의 80%이상이 AXEase 단백질(33.5 kDa)였다. 이러한 결과는 9.8 g/l의 AXEase 단백질을 배양 상등액으로 대량 분비ㆍ생산할 수 있음을 의미한다.

Long-Term Investigation of Regional Topographic Effects on Soil Chemical Properties and Heavy Metal Concentrations in Paddy Fields

  • Ahn, Byung-Koo;Kang, Seong-Soo;Shin, Jae-Yeon;Lee, Jin-Ho
    • 한국토양비료학회지
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    • 제45권5호
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    • pp.738-743
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    • 2012
  • Topographic conditions of agricultural fields work as a important factor to identify different soil properties. This study was conducted to investigate the selected soil chemical properties and the concentrations of heavy metals, Cd, Cr, Pb, Cu, Ni, and Zn, in the paddy fields of different topographic areas at four year intervals from 1999 to 2011. Three-hundred soil sampling sites in the paddy fields were selected from the different topographic areas that were local valley and fans, fluvio-marine deposits, alluvial plains, and diluvial terraces. The mean values of soil pH ranged 5.7~5.8 that were within optimal range for rice cultivation. The mean values of other properties such as soil organic matter (SOM) content, the concentrations of exchangeable cations, $K^+$, $Ca^{2+}$, and $Mg^{2+}$, and available silicate concentration were lower or close to the optimal values, but the mean concentrations of available phosphorus were exceeded the range of optimal value, $80{\sim}120mg\;kg^{-1}$, in many paddy fields. In particular, The concentrations of available phosphorus in the paddy fields of local valley and fans, alluvial plains, and fluvio-marine plains were mostly declined. However, in diluvial terrace areas, the phosphorus concentrations unexpectedly increased; furthermore, they were significantly higher than those in other topographic areas. The mean concentrations of 0.1 M HCl-extractable heavy metals, Cd, Cr, Pb, Cu, Ni, and Zn, in the paddy fields were slightly and gradually declined during the study years, but the Pb concentrations were not statistically changed. In addition, the concentrations of heavy metals were widely ranged depending on the different sampling sites. Nevertheless, the concentrations of heavy metals were significantly lower than the levels of Soil Contamination Warning Standard (SCWS) for agricultural lands (1-region) presented in Soil Environment Conservation Law (SECL).

테크노파크 조성사업의 성과와 정책과제 (Performance and Alternative Policies of Techno-Park Built-up Project in Korea)

  • 이철우;김명엽
    • 한국경제지리학회지
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    • 제12권1호
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    • pp.19-37
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    • 2009
  • 우리나라의 테크노파크 조성사업은 지역의 기술혁신촉진, 산업구조 고도화 등을 위한 기업지원 플랫폼으로서 시작되었다. 1997년 6개를 시범사업으로 시작된 테크노파크는 현재 전국 15개 시도에 17개가 지정되어 창업보육, 시험생산, 교육 훈련 등의 다양한 기업지원서비스를 제공하고 있다. 지역혁신사업 추진체계의 개편과 "산업기술단지지원에 관한 특례법" 개정, 2단계 테크노파크 조성사업 추진은 테크노파크가 지역의 기술혁신거점기능을 수행하고, 기술기업을 육성하는데 있어 핵심적인 정책 제도 사업적 기반으로 작용하고 있다. 앞으로 테크노파크가 지역에서 기술혁신거점기능을 강화하고 기술 기업육성의 중심기관으로 자리 잡기 위해서는 산 학 연 관과의 네트워크 활성화, 지역산업육성의 기획기능 강화, 지역 중소기업 지원의 단일창구 역할정립 등의 과제해결이 필요하다.

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파프리카 재배에서 계절별 광환경 조건과 증산량 예측에 근거한 관수공급 기준 제시 (Irrigation Criteria based on Estimated Transpiration and Seasonal Light Environmental Condition for Greenhouse Cultivation of Paprika)

  • 신종화;손정익
    • 생물환경조절학회지
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    • 제24권1호
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    • pp.1-7
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    • 2015
  • 파프리카는 수분에 민감한 작물이므로 작물의 생산성 향상을 위하여 적정 관수조절은 매우 중요하다. 광환경 조건은 시설재배에서 여러 환경 변수 중 조절이 용이하지 못하며, 지역 별, 계절 별 분포가 다르기 때문에 광환경 데이터를 이용한 증산과 관수의 추정이 필요하다. 본 연구에서는 파프리카의 정확한 증산 예측을 위하여 변형된 증산 추정식을 활용하였다. 또한 기상청의 광도자료를 활용하여 지역 별 증산량과 관수량을 비교하였다. 우리나라의 경우 여름철 하루 중 광도의 편차가 심하고 장마기간이 있으므로 봄, 가을에 비하여 증산량이 오히려 낮았다. 그리고 광주기가 길어지는 봄에 증산량이 가장 많았으므로, 이 시기의 데이터를 이용하여 관수시설 용량을 지역별로 제시할 수 있었다. 이러한 결과는 시설재배에서 관수설비 기준제시를 위한 자료 및 투입에너지 최적화에도 유용하게 활용될 것으로 판단된다.

오이의 배발생 현탁 배양세포로부터 제초제 저항성 형질전환 식물체 생산 (Production of Herbicide-resistant Transgenic Plants from Embryogenic Suspension Cultures of Cucumber)

  • 우제욱;정원중;최관삼;박효근;백남긴;유장렬
    • 식물조직배양학회지
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    • 제28권1호
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    • pp.53-58
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    • 2001
  • 제초제 저항성 오이 (Cucumis sativus L. cv Green angel)를 생산하기 위하여 배발생 현탁배양세포와 binary vector pGA-bar을 지닌 Agrobacterium tumefacians (LBA4404)를 공동배양하였다. 형질전환 벡터의 T-DNA부분에는 kanamycin에 저항성을 나타내는 neomycin phosphotrans ferase (npt II) 유전자와 phosphinothricin (PPT)에 저항성을 나타내는 phosphinothricin acetyltransferase (bar) 유전자를 지니고 있다. 48시간의 공동배양 후 배발생 캘러스는 20mg/L PPT가 함유된 성숙배지에서 배양하였다. 약 200개체의 형질전환 유식물체를 40mg/L PPT가 첨가된 호르몬이 없는 배지에서 생산하였다. 5개의 오이 형질전환 식물체의 염색체에 bar유전자가 도입되어 발현되는 것을 northern blot 분석을 통하여 확인하였다. 형질전환 오이 식물체가 토양에서 성숙되었다. 성숙한 오이 식물체는 PPT가 함유된 상업적 제초제 (Basta)를 일반적인 사용 농도 (3ml/L)처리시에도 저항성을 나타내며 생장하였다.

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Effects of growing degree days on growth and yield of maize depending on the sowing date

  • Jung, Gun Ho;Kim, Mi Jung;Son, Beom Young;Shin, Seong Hyu;Kim, Sung Kook;Lee, Jae Eun;Kim, Chung Guk;Kwon, Young Up;Shim, Kang Bo;Heu, Sunggi
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.324-324
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    • 2017
  • Total 15 different corn hybrids, Kwangpyeongok, Gangdaok, Yanganok, Singwangok, Jangdaok, Cheonganok, Cheongdaok, Andaok, Dapyeongok, Pyeongkangok, Pyeonganok, Daanok, Sunwon P3394, Gangilok, P3394, had been used to investigate the plant growth and yield of corn hybrids depends on the sowing date. Sowing dates were April 5th, June 25th, and July 5th and all experiments had three repeats. The growth of Gangdaok was the best. However, in the case of Kwangpyeongok, the growth was not the best to compare with Gangdaok, but the stem to ear height ratio was lower than Gangdaok, it may be better for the stable cultivation. Both of the growth and yield of Daanok was not good regardless of planting date, but the yield and ear shape of Pyeongkangok and Dapyeongok were good for fresh corn. The growth and yield of 15 different corn hybrids were variable depends on the planting date, however, the growth degree days (GDD) was the most important factor for the maturity of the corn. More than $1500^{\circ}C$ of GDD was enough to harvest mature corn hybrids in the central region of Korea. Besides the yield and growth, other characters such as sweetness and taste as a food should be investigated further for the fresh corn to be suggested.

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Overexpression of OsNAC17 enhances drought tolerance in rice

  • Kim, Tae Hwan;Kim, Ju-Kon
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
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    • pp.168-168
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    • 2017
  • Drought conditions during cultivation reduce agricultural production yield less than a theoretical maximum yield under normal condition. Plant specific NAC transcription factors in rice are known to play an essential roles in stress resistance transcriptional regulation. In this study, we report the rice (Oryza sativa L japonica) NAM, AFTF and CUC transcription factor OsNAC17, which is predominantly induced by abiotic stress in leaf, was contribute to the drought tolerance mediated reactive oxygen species (ROS) in transgenic rice plants. Constitutive (PGD1) promoter was introduced to overexpress OsNAC17 and produced the transgenic PDG1:OsNAC17. Overexpression of OsNAC17 throughout the whole plant improved drought resistance phenotype at the vegetative stage. Morphological characteristics such as grain yield, grain filling rate, and total grain weight improved by 22~64% over wild type plants under drought conditions during the reproductive stage. The improved drought tolerance in transgenic rice was involved in reducing stomatal density up to 15% than in wild type plants and in increasing reactive oxygen species-scavenging enzyme. DEG profiling experiment identified 119 up-regulated genes by more than twofold (P<0.01). These genes included UDP-glycosyltransferase family protein, similar to 2-alkenal reductase (NADPH-dependent oxireductase), similar to retinol dehydrogenase 12, Lipoxygenase, and NB-ARC domain containing protein related in cell death. Furthermore, OsNAC17 was act as a transcriptional activator, which has an activation domain in C-terminal region. These result demonstrate that the overexpression of OsNAC17 improve drought tolerance by regulating ROS scavenging enzymes and by reducing stomatal density

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