• 한국작물학회지
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• 제62권3호
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• pp.166-171
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• 2017

본 실험은 적미의 재배에 있어서 고품질 고수량을 위한 적정이앙시기를 구명하기 위해 네 품종의 적미를 재료로 2015~2016년에 밀양에서 실시하였다. 1. 수량구성요소 중 이앙시기 변화에 따라 가장 크게 달라지는 요인은 등숙률이었고 6월 20일 이후 이앙하면 등숙률이 낮아져 현미수량이 하락하였다. 2. 6월 20일에 이앙했을 때 폴리페놀 함량과 현미수량을 종합한 폴리페놀 생산량이 2,341~3,349 g/10a로 가장 높았다. 3. 출수 후 30일간 평균온도가 낮을 수록 폴리페놀 함량은 증가하였으나 일정 수준 이하로 온도가 떨어지면 현미수량이 감소하였기 때문에 $22{\sim}23^{\circ}C$ 정도가 고품질 고수량 적미를 생산하기에 가장 적정한 온도였다. 4. 출수 후 30일간 평균온도가 $22{\sim}23^{\circ}C$가 되는 시점을 최적 출수기로 하고 출수소요적산온도를 $1,660^{\circ}C$로 하여 계산한 최적 이앙시기는 밀양시가 6월 18일~6월 24일 이었다. 경상남도 지역은 6월 중순에서 7월 초순, 경상북도 지역은 6월 초순에서 6월 중하순이 최적 이앙기였다.

• 원예과학기술지
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• 제18권1호
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• pp.9-13
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• 2000

플러그묘를 이용하여 불량 기상환경을 회피하며 양파 수급의 안정을 위해 중부지방에서의 양파 생산 가능성을 검토하였다. 정식시기와 육묘일수 실험의 경우 '패총황'을 공시하여 3월 15일과 4월 15일에 162공 트레이에 30, 60, 90일간 육묘후 정식하였으며 적정 육묘 용기 선택은 cell 크기별로 '패총황'을 90일간 육묘해서 3월 15일에 정식하였고 춘파재배시 적품종을 선발코자 '패총황'외 조 중 만생계 6품종을 공시하여 90일간 육묘후 3월 15일에 정식하였다. 플러그묘의 정식시기에 따른 육묘일수는 3월 15일 정식한 60일 묘가 107.2ton/ha로 가장 수량이 많았는데 정식시기에 따라서는 수량에는 유의차가 없고 육묘일수에 따라 차이를 보였다. 육묘용기로는 128공에서 125.0ton/ha로 수량이 가장 많았으며 400공의 경우 미성숙 상태로 수확이 되어 적합치 않은 것으로 사료되며 육묘시 단위 면적당 육묘본수를 고려한다면 200공이나 288공까지도 가능할 것으로 보인다. 품종에 따른 수량은 중생종인 '천주황'이 130.5ton/ha로 가장 많았으나 중부지방의 기후적 여건을 감안할 경우 다소 수량이 적지만 숙기가 빨라 6월 중순 이전에 수확할 수 있는 '용봉황' 등의 조생종 계통이 유리할 것을 사료된다.

• Journal of Microbiology and Biotechnology
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• 제12권6호
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• pp.989-992
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• 2002

Sweet persimmon (Diospyros kaki Thunb.) is widely cultivated in the southern part of Korea and its cultivation is increasing. However, anthracnose disease caused by Colletotricuhum species is one of the major hinderances to the cultivation and production of sweet persimmon. Therefore, in the current study, PCR was used to specifically detect Colletotrichum spp., based on the sequences of the ITS II regions in the rDNA. Using the sequence data, CO-1 was designated to detect Colletotrichum together the with ITS 4 primer. The result showed that a single segment of ca. 500 bp was observed only in Colletotrichum, but not in any other fungal and bacterial isolates. The annealing temperatures and template DNA quantites were also investigated to identify optimal conditions for detection. Using these species-specific primers, a unique band was obtained at annealing temperatures ranging from $55^{\circ}C\;and\;61^{\circ}C$ and template DNA levels from 10 pg- $10{\mu}g$.

• 농촌계획
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• 제20권4호
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• pp.45-54
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• 2014

The main purpose of this study is to provide the back-from-city farmers with the information about the melon cultivation technology by surveying 268 farm houses in the major melon producing districts such as Seongju and Chilgok. For the purpose, this study classifies the essential technologies that the melon experts think as most important into 6 categories: size of plastic film house, covering film, varieties of oriental melon, lagging cover, ventilation method and ways to reduce repeated-cultivation damage. The result of the study shows that the back-from-city farmers should consider the following items when they choose to cultivate oriental melons. For the size of plastic film house, the ventilation method and the covering film of plastic film house, it is better to choose the latest technology. Even though it may require larger initial investment, the latest technology can increase the production and lower the cost. In case of variety, it is better to choose popular or the most widely grown ones rather than the new ones. The lagging cover should be selected in consideration of climate conditions such as average temperature and humidity, transplant time and harvest time of the farming region.

• 한국토양비료학회지
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• 제44권4호
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• pp.534-538
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• 2011

One of the problems for cultivating crops in the mountainous highland is soil erosion and nutrients runoff. Alternative cropping ways were searched to reduce soil erosion and to ensure farm income in the mountainous highland agricultural region. Three edible wild plants including goatsbeard, Korean thistle, and aster, were selected to test as alternative crops to reduce soil erosion in mountain agriculture of highland area. In the first year, the soil losses from the alternative cropping were 26 to 63 percents of the soil loss from summer radish cultivated by conservation tillage with contour and plastic film mulching. The relative soil losses in the second year ranged from 2.8 to 5.5 percents in comparison with radish cultivation. Rapid surface coverage contributed to successive soil loss protection by these alternative crops. Farm net profit of these crops was greater than that of radish. Monitoring of yields of Korean thistle or aster for further experiments, however, might be necessary for economic cultivation due to yield reduction caused by consecutive production.

• Mycobiology
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• 제47권1호
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• pp.76-86
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• 2019

Scab disease caused by Venturia nashicola is of agroeconomic importance in cultivation of Asian pear. However, little is known about the degree of genetic diversity in the populations of this pathogen. In this study, we collected 55 isolates from pear scab lesions in 13 major cultivation areas in Korea and examined the diversity using sequences of internal transcribed spacer (ITS) region, ${\beta}$-tubulin (TUB2), and translation elongation factor-$1{\alpha}$ ($TEF-1{\alpha}$) genes as molecular markers. Despite a low level of overall sequence variation, we found three distinctive subgroups from phylogenetic analysis of combined ITS, TUB2, and $TEF-1{\alpha}$ sequences. Among the three subgroups, subgroup 1 (60% of isolates collected) was predominant compared to subgroup 2 (23.6%) or subgroup 3 (16.4%) and was distributed throughout Korea. To understand the genetic diversity among the subgroups, RAPD analysis was performed. The isolates yielded highly diverse amplicon patterns and none of the defined subgroups within the dendrogram were supported by bootstrap values greater than 30%. Moreover, there is no significant correlation between the geographical distribution and the subgroups defined by molecular phylogeny. Our data suggest a low level of genetic diversification among the populations of V. nashicola in Korea.

• 한국농공학회논문집
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• 제62권3호
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• pp.51-61
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• 2020

Drought events are not clear when those start and end compared with other natural disasters. Because drought events have different timing and severity of damage depending on the region, various studies are being conducted using satellite images to identify regional drought occurrence differences. In this study, we investigated the applicability of drought assessment using the Evaporative Stress Index (ESI) based on Moderate Resolution Imaging Spectroradiometer (MODIS) satellite images. The ESI is an indicator of agricultural drought that describes anomalies in actual and reference evapotranspiration (ET) ratios that are retrieved using remotely sensed inputs of Land Surface Temperature (LST) and Leaf Area Index (LAI). However, these approaches have a limited spatial resolution when mapping detailed vegetation stress caused by drought, and drought hazard in the actual crop cultivation areas due to the small crop cultivation in South Korea. For these reasons, the development of a drought index that provides detailed higher resolution ESI, a 500 m resolution image is essential to improve the country's drought monitoring capabilities. The newly calculated ESI was verified through the existing 5 km resolution ESI and historical records for drought impacts. This study evaluates the performance of the recently developed 500 m resolution ESI for severe and extreme drought events that occurred in South Korea in 2001, 2009, 2014, and 2017. As a result, the two ES Is showed high correlation and tendency using Receiver Operating Characteristics (ROC) analysis. In addition, it will provide the necessary information on the spatial resolution to evaluate regional drought hazard assessment and and the small-scale cultivation area across South Korea.

• Journal of Ecology and Environment
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• 제29권3호
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• pp.227-235
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• 2006

강원도 평창군 일대에서 화전 후 묵밭의 식생 천이에 따른 식생 구조의 발달 과정과 종다양성의 변화를 조사하였다. 방치 후 경과 년 수에 따라 최상층부의 키는 초기에 빠르게 증가하고 후기에 안정화하는 경향을 나타내었다. 10, 20, 50 및 80 년차 묵밭에서 최상층부 식생의 키는 각각 4, 8, 18 및 18 m이었다. 교목층, 아교목층, 관목층 및 초본층의 4층 구조를 갖춘 완전한 형태의 숲으로 발달하기까지는 방치 후 약 35년이 경과해야 하는 것으로 추정되었다. 방치 후 경과 년 수에 따라 식생치 Ivc는 로그 함수적으로 그리고 식생치 Hcl은 직선적으로 증가하는 경향이 있었다. 묵밭의 식생천이에서 종다양성은 천이 중기인 $10{\sim}20$년차에서 최고로 증가한 다음 이후 점차 감소하는 경향을 나타내었다. 본 조사지에서 종다양성은 균등도보다 종풍부도와 매우 밀접한 상관을 나타내었다. 대부분의 목본은 개망초-쑥 단계인 $2{\sim}6$년차에 정착이 이루어져 시간 경과에 따라 이들의 우점도가 변화함으로써 Egler (1954)의 초기 종조성 모델을 따르는 것으로 확인되었다.

• 생약학회지
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• 제43권3호
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• pp.231-238
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• 2012

Menthae herba (MH) extracts exhibit anti-inflammatory effects. The purpose of this study was to determine whether the anti-inflammatory effects of MH extracts vary according to the cultivation regions. We performed a comparative analysis of MH extracts by evaluating the production of inflammatory mediators in RAW 264.7 murine macrophage cells and HaCaT human keratinocyte cells. MH extracts obtained from different cultivation regions in Korea and China significantly reduced the production of nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$), and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) in RAW 264.7 cells stimulated with lipopolysaccharide (LPS). No differences in these inhibitory activities were observed between MH extracts. In HaCaT cells stimulated with TNF-${\alpha}$ and interferon-${\gamma}$ (IFN-${\gamma}$), MH extracts did not inhibit the production of macrophage-derived chemokine (MDC/CCL22), but most extracts reduced the production of the regulated on activation normal T-cell expression and secreted (RANTES/CCL5). We used clustering tree analysis of the MH extracts according to the chromatographic pattern and anti-inflammatory potency of MH extracts. We observed differences in the chromatographic pattern of MH extracts but no difference in anti-inflammatory potency. Our findings suggest that MH extracts from different regions do not show any differences in their pharmacological potency in that MH extracts are used as therapeutic agents to treat inflammatory disorders.

• Journal of Applied Biological Chemistry
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• 제62권2호
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• pp.129-135
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• 2019

Salvia miltiorrhiza Radix is cultivated in Korea and China and is traditionally used to treat cardiovascular diseases. In this study, we developed and validated a quantitative analysis method for S. miltiorrhiza Radix using high-performance liquid chromatography (HPLC). Identification was performed using ultra performance liquid chromatography-tandem mass spectrometry. For quantitative analysis, we used seven marker compounds. Separation conditions for HPLC were optimized using an ODS column with gradient conditions of 1% formic acid in distilled water and 1% formic acid in acetonitrile, with a flow rate of 0.8 mL/min and a detection wavelength of 280 nm. This method showed good linearity ($R^2=0.9998$), precision (relative standard deviation ${\leq}3.3%$), accuracy (recovery of 94.16-102.89%), limit of detection ($7.53{\mu}g/mL$), and limit of quantification ($23.71{\mu}g/mL$). This approach successfully quantified marker compounds in S. miltiorrhiza Radix. The individual marker compounds were identified by comparing the molecular masses and retention times with does standard compounds. Marker compound contents of S. miltiorrhiza Radix were investigated with different cultivation regions. Seven marker compounds were detected and quantified in all samples. Among them, salvianolic acid B showed the highest contents and it ranged from 4.13 to 7.15%. The salvianolic acid B content (7.15%) of marker compound was the highest in Bonghwa, and the tanshinone IIA content (1.90%) was the highest in Pohang. The results of marker compounds and developed method were intended to provide a favorable reference for the study of S. miltiorrhiza Radix from different regions of Korea.