• Journal of Biosystems Engineering
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• v.20 no.1
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• pp.73-86
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• 1995

The automatic control system in the greenhouse have to be developed to the direction of considering various factors the variables such as condition of the cultivation and greenhouse, the properties and types of products. Therefore, it is more important to set up variables appropriately than the problems of automatic control system itself, and the automatic control system which satisfy these problems should be simplified in the aspect of operation. In addition, even the individual automations are not perfect yet, so more studies are required for the development of comprehensive automatic system in korea. This study was carried out to automatize environment control systems for greenhouse, especially from most intensive labor requiring parts such as watering, irrigating liquefied fertilizer, spraying chemicals, mixing and ventilation system, etc. The results are summarized as follows. 1. Control type tensiometer was expected to be desirable in the automation of watering system, therefore, a new tensiometer was designed and developed through this study. 2. The chemical spraying system developed through this study was found to be excellent in the aspect of operation. 3. When pulse type water discharge meter was used in the mixing of liquefied fertilizer and chemical solution, the error of mixing were range $\pm$0.1~0.15%. 4. The water level switch of electrod type used for controlling water level was found to be affective in both control performance and operation cost. 5. The water and level control system can be omitted if each tank size are standardized in accordance with greenhouse size, therefore, the installation cost might be significantly reduced. 6. The developed general controller was excellent in hardware parts, but still remained to be improved in software parts.

• Journal of The Korean Society of Grassland and Forage Science
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• v.4 no.2
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• pp.158-163
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• 1983

The purpose of this experiment was to find out fundamental data for the cultivation of sorghum-sudangrass hybrid. Leaf area index and dry matter accumulation were investigated with 3-levels of nitrogen application,15, 25, 35kg per 10a during the period of initial growth from the 8th leaf emergence to the late blooming stage. The results obtained were as follows: Leaf emergence increased as growth progressed and in the same pattern at all N-levels. After the heading stage, the value was 15 at all N-levels. Leaf area index (LAI) increased as growth progressed and rapidly till the booting stage. At 15kg N-level the values were larger till the ear formation stage, and at 35tg N-level the values were larger than other N-levels after the booting stage. At the late blooming stage LAI reached 8.13 at 35kg N-level. The value of dry matter accumulation was the highest at 35kg N-level at the late blooming stage, and as was the same in the value of crop growth rate (CGR). There was a tendency that before the booting stage the enlargement of leaf area contributed to dry matter accumulation and after the booting stage did the increase of net assimilation rate (NAR) .

• The Korean Journal of Malacology
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• v.27 no.3
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• pp.205-211
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• 2011

This study presents physiological rates of oxygen consumption, ammonia excretion, feeding rates, O/N ratio and assimilation efficiency of the blood cockle, Scapharca subcrenata, determined from specimens collected in Yeoja bay on the south coast of Korea. Physiological parameters were measured monthly under static, laboratory controlled conditions with ambient conditions, and measurements were performed seasonally in order to estimate scope for growth and its probable sources of variation. Oxygen consumption and ammonia excretion rates have been increased as temperature increased with the highest value of August, 2008. Feeding rate was the highest during April whereas the lowest was during August which is a period of gametogenesis with minimum biomass of phytoplankton around sampling area. Assimilation efficiency was not significantly different seasonally and O/N ratio decreased during July to August. The scope for growth was negative during high temperature months(July to August), reflecting the high temperature and low feeding rate, and had its highest positive values during spring and autumn. Data on the physiological parameters and scope for growth of Scapharca subcrenata obtained in this study will be used to assess the carrying capacity for blood cockle cultivation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.29 no.3
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• pp.218-226
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• 1984

This study was conducted to establish fundamental of cultivation system in the southern warm region of Korea by investigation of variation of yield component and yield under the different nitrogen fertilizing levels. And that levels in this experiment were 10, 15, 20 and 25 Kg/10a. Optimum fertilizing level of nitrogen was 20 kg/10a in Seokwang variety and 25 kg/ 10a in Dongjin variety. On the optimum fertilizing level (Seokwang; 20 kg/10a, Dongjin; 15 kg/10a), the correlation coefficient between No. of panicles per unit area and the rate of ripened grains were showed negative correlation (Seokwang; r =-0.6023$\^$*/, Dongjin; r=-0.858l$\^$**/). In the primary, secondary branches and spikelets, the degeneration ratio was increased significantly under the level of N=25 kg/10a in Seokwang, N$\geq$20 kg/10a in Dongjin. In Dongjin, the rate of ripened grains was decreased with increase in nitrogen fertilizing levels and that caused to decrease the yield.

• Journal of Fisheries and Marine Sciences Education
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• v.28 no.2
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• pp.384-390
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• 2016

The purpose of this paper is to improve the availability of underwater sound by the fundamental data on the hearing ability of Redlip croaker Pseudosciaena polyactis, which is cultured according to the cultivation technology, recently. The auditory thresholds of Redlip croaker were determined at 6 frequencies from 80Hz to 800Hz by heartbeat conditioning method using pure tones coupled with a delayed electric shock. The audible range of the Redlip croaker extended from 80Hz to 800Hz with the best sensitive frequency range including little difference in hearing ability from 80Hz to 500Hz. In addition, the auditory thresholds over 800Hz increased rapidly. The mean auditory thresholds of the Redlip croaker at the test frequencies from 80Hz to 800Hz were 90.7dB, 93.4dB, 92.9dB, 94.4dB, 95.5dB and 108dB, respectively. Auditory masking for the redlip croaker was measured using masking stimuli with the spectrum level range of about 66, 71, 75dB (0dB re $1{\mu}Pa/{\sqrt{Hz}}$). According to white noise level, the auditory thresholds increased as compared with thresholds in a quiet background noise. The Auditory masking by the white noise spectrum level was stared over about 70dB within 80~500Hz. Critical ratio ranged from minimum 20.7dB to maximum 25.5dB at test frequencies of 80Hz~500Hz.

• Journal of Environmental Science International
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• v.31 no.3
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• pp.265-274
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• 2022

Lipoxygenase is an enzyme, mainly produced by plants, capable of converting unsaturated fatty acids to fatty acids. It has vast application potential in the food, pharmaceutical and agricultural industries. The aim of this study was to isolate novel lipoxygenase-producing bacteria from the environment and to investigate the lipoxygenase enzymatic properties for industrial production. The strain, NC1, isolated from cultivation soils, was identified as Bacillus subtilis based on the phenotypic characteristics and 16S rRNA gene sequencing. This strain formed a pink color around the colony when cultured on indamine dye formation plates. The production of lipoxygenase by B. subtilis NC1 was influenced by the composition of the medium and linoleic acid concentrations. The optimum temperature and pH for lipoxygenase activity was determined to be 40 ℃ and pH 6, respectively. The enzyme showed relatively high stability at temperatures ranging from 20-50 ℃ and acid-neutral regions. In addition, the lipoxygenase produced by B. subtilis NC1 was able to degrade commercially available oils including sunflower seed oil and Perilla oil. In this study, a useful indigenous bacterium was isolated, and the fundamental physicochemical data of bacterial lipoxygenase giving it industrial potential are presented.

• Korean Journal of Ecology and Environment
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• v.49 no.2
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• pp.89-101
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• 2016

To elucidate occurrence patterns of $C_4$ plants in agroecosystems, we surveyed flora inside fields and embankments in 222 sites in farming lands in summer and fall, 2013. We also observed Kranz anatomy and analyzed carbon stable isotope to classify photosynthesis types. Our study observed total 54 species of $C_4$ plants in 33 genera and 9 families of vascular plants besides cultivated crops occurring in farming fields. The most common species was Portulaca oleracea L. followed by Digitaria ciliaris and Echinochloa crusgalli (L.) P. Beauv. var. crusgalli among the $C_4$ plants. The ratios of $C_3$ and $C_4$ species number to total number of species tended to maintain constant regardless of survey times and habitat types. The proportion of $C_4$ species to the total number of species were more influenced by the number of $C_3$ species than those of other types in the surveyed sites. Sizes of cultivation areas did not affect the proportions of $C_4$ plants. Increasing number of simultaneous weeding methods, especially with cutting method, appeared to decrease the number of $C_3$ plants. On the other hand, as the number of cultivated crops increased, both $C_3$ and $C_4$ plants increased but the proportions of $C_4$ species remained. We interpret our results that proportion of $C_4$ species in agroecosystems, appear to be dependent on the number of $C_3$ species, which in turn, appear to be affected by cultivation methods.

• Journal of the Korean Wood Science and Technology
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• v.39 no.1
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• pp.68-74
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• 2011

Cauliflower mushroom ($Sparassis$ $crispa$) is being cultivated as a functional mushroom since the mushroom contains larger amount of ${\beta}$-glucan than other edible or medicinal mushrooms. In this study, as a fundamental study for the cultivation process of cauliflower mushroom, we assayed the mycelial growth and the productivity of the mushroom cultivated on the sawdust-based medium made of larch ($Larix$ $kaempferi$) by 4 kinds of medium densities and 6 kinds of particle sizes. Low densities of sawdust-based media (0.68~0.72 g/$cm^3$) showed high variations in mycelial growth at early stage on sawdust-based medium. The coefficient of variance for the mycelial growth decreased from higher than 40% at the 3rd week to lower than 10% at the 7th week. High density of sawdust-based medium (0.80 g/$cm^3$) showed relatively lower variation in the mycelial growth at early stage with less than 30% of coefficient of variance, the high density of sawdust-based medium was thought to be quite stable compared to the lower densities of sawdust-based medium. From the viewpoint of mushroom productivity especially for the goods (excluding bottom of fruiting body), 0.76 g/$cm^3$ was better than any other densities; the return rates of fruiting body from each medium were 12.2, 13.6, 13.1, and 12.0% for 0.80, 0.76, 0.72, and 0.68 g/$cm^3$, respectively. By the way, it took about 40 days for harvesting from primordium formation with the sawdust-based medium excluded the particles less than 1 mm, while it took about 70 days with the medium including the particles less than 1 mm. The yield from the sawdust-based medium with the particles less than 1 mm was also quite less than any other sawdust-based medium especially for the goods. Therefore, we recommend that the sawdust-based medium with larch for the cultivation of cauliflower mushroom be prepared as 0.76 g/$cm^3$ in medium density with excluding the particles less than 1 mm.

• Journal of Wetlands Research
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• v.11 no.3
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• pp.49-59
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• 2009

Seasonal changes of the growth characteristics and biomass of Scirpus fluviatilis, a aquatic emergent vascular plant, were investigated to reveal the phenology and the population dynamics and to provide the fundamental resources for the restoration counterplan of the wetland vegetation in the littoral zone of the Upo wetland, Changnyeong-gun, Gyeongsangnam-do, Korea from March 2006 to November 2006. Scirpus fluviatilis was distributed commonly in Upo, Mokpo, Sajipo, Jokjibyeol, and Topyeongcheon upstream and downstream of Upo wetland, and the density was highest in Mokpo. Distribution range for the water depth was 9~49cm, and the highest shoot density in 26~49cm, and the mean shoot density was $119/m^2$, and the mean shoot length was 122.3cm on May 28. The number of the tuber was $104.5/0.25m^2$, and the living tubers were 84.2%. The mean fresh biomass of the living tubers was 3.0g, and those of 1~4g was most as 57.9%. Germination rates of the living tubers was 43.8%, and the maximum rate was in 7~9g and more than 10g. In the pot cultivation, the shoot density of the germinated tubers and the dormant tubers were highest as 13.5 and 9.7, respectively in early August. In the field study, the shoot density had few change before typhoon damage, while the density increased abruptly in November after flooding accompanied with the typhoon 'Ewiniar'. The shoot length in the pot cultivation and in the field study were 100~116cm and 60~170cm, respectively in the growth-end. Biomass allocation rates into the stem, leaf, flower, and underground parts were 8.9%, 6.6%, 0%, and 84.5%, respectively in the pot cultivation of the germinated tubers, and those of the dormant tubers were 7.1%, 7.1%, 0%, and 85.8%, respectively. The tuber number increased to 1.4~4.1 times by the growth-end, so it is concluded that Scirpus fluviatilis is mostly propagated by the vegetative reproduction.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.6
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• pp.880-884
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• 2001

Rumen microbiology research has undergone several evolutionary steps: the isolation and nutritional characterization of readily cultivated microbes; followed by the cloning and sequence analysis of individual genes relevant to key digestive processes; through to the use of small subunit ribosomal RNA (SSU rRNA) sequences for a cultivation-independent examination of microbial diversity. Our knowledge of rumen microbiology has expanded as a result, but the translation of this information into productive alterations of ruminal function has been rather limited. For instance, the cloning and characterization of cellulase genes in Escherichia coli has yielded some valuable information about this complex enzyme system in ruminal bacteria. SSU rRNA analyses have also confirmed that a considerable amount of the microbial diversity in the rumen is not represented in existing culture collections. However, we still have little idea of whether the key, and potentially rate-limiting, gene products and (or) microbial interactions have been identified. Technologies allowing high throughput nucleotide and protein sequence analysis have led to the emergence of two new fields of investigation, genomics and proteomics. Both disciplines can be further subdivided into functional and comparative lines of investigation. The massive accumulation of microbial DNA and protein sequence data, including complete genome sequences, is revolutionizing the way we examine microbial physiology and diversity. We describe here some examples of our use of genomics- and proteomics-based methods, to analyze the cellulase system of Ruminococcus flavefaciens FD-1 and explore the genome of Ruminococcus albus 8. At Illinois, we are using bacterial artificial chromosome (BAC) vectors to create libraries containing large (>75 kbases), contiguous segments of DNA from R. flavefaciens FD-1. Considering that every bacterium is not a candidate for whole genome sequencing, BAC libraries offer an attractive, alternative method to perform physical and functional analyses of a bacterium's genome. Our first plan is to use these BAC clones to determine whether or not cellulases and accessory genes in R. flavefaciens exist in clusters of orthologous genes (COGs). Proteomics is also being used to complement the BAC library/DNA sequencing approach. Proteins differentially expressed in response to carbon source are being identified by 2-D SDS-PAGE, followed by in-gel-digests and peptide mass mapping by MALDI-TOF Mass Spectrometry, as well as peptide sequencing by Edman degradation. At Ohio State, we have used a combination of functional proteomics, mutational analysis and differential display RT-PCR to obtain evidence suggesting that in addition to a cellulosome-like mechanism, R. albus 8 possesses other mechanisms for adhesion to plant surfaces. Genome walking on either side of these differentially expressed transcripts has also resulted in two interesting observations: i) a relatively large number of genes with no matches in the current databases and; ii) the identification of genes with a high level of sequence identity to those identified, until now, in the archaebacteria. Genomics and proteomics will also accelerate our understanding of microbial interactions, and allow a greater degree of in situ analyses in the future. The challenge is to utilize genomics and proteomics to improve our fundamental understanding of microbial physiology, diversity and ecology, and overcome constraints to ruminal function.